Rmu_sc0004928.1_g000028

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004928.1
N/A
Physical Location & Seq
Reverse (-)
102979 .. 104960
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 906 bp
atgcatagaaatgcgtgtaatcagctgccgaaggaggtaaaggccgagtattacagcagggatccgatgaggaaagttaagttctttagtagctttaatctattcgagttagctaattggagagacagtttgtatgttgacatggctccagagcctcttgatcctcaaaaactgcctatggtttgcaggaccacatcggtacccagtgaccaagcactcctgcccccaaaagaggagaccgaacagccgccatgttgtcgagaacactactggatagcaattctaagtttaccaccctacccactactgcttgaccaccttataaggtggcattcattgcttagccactactatccaccatgccctgagcctgaactcaccatgggcaccggcaaacattttgatgttgcttttcgcaccatcctacttcaggaccacatcgggtcggggatggatggaaccaatcagaagtgcaaatcacgcaagcgaagcgaccgcaccgaaacgggattcccaattcaataccaaaacaaatggattgatgttcctcctatgcctaaagctctagtggtgaacgtttgggatttcttacgttgctgctccggcctcgatcaagcggtgacggcgcggcgcggctttccggagagaggtcaggggtcgtgcggaggagtttgcggtcgggtggagtcgccggcgacacgaaaaagtgcagacgttgctgctccggcctcgatcaagcggcgacggcgcggcgcggctttccggagagaggtcaggggtcgtgcggaggagtttgtggtcgggtggagtcgccggcgacgggttttgcagtgctgcatagaacctgtatttgcaggtgaggtggctacccagaaactggcaaccctaacctcctccgacctctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

301

Amino Acids

34.25

Weight (kDa)

9.16

Isoelectric Point (pI)

70.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 111 - 198 3.5e-11 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 323
AarI CACCTGC 1 cut(s) 845
Acc36I ACCTGC 1 cut(s) 845
Acc65I GGTACC 1 cut(s) 199
AccB1I GGYRCC 2 cut(s) 199, 386
AccB7I CCANNNNNTGG 1 cut(s) 877
AccII CGCG 4 cut(s) 628, 633, 750, 755
AccIII TCCGGA 2 cut(s) 640, 762
AclI AACGTT 1 cut(s) 576
AclWI GGATC 3 cut(s) 56, 69, 155
AcuI CTGAAG 1 cut(s) 413
AfaI GTAC 1 cut(s) 201
AfiI CCNNNNNNNGG 6 cut(s) 232, 430, 647, 769, 820, 877
AgsI TTSAA 1 cut(s) 521
AluBI AGCT 4 cut(s) 25, 93, 113, 563
AluI AGCT 4 cut(s) 25, 93, 113, 563
Alw26I GTCTC 2 cut(s) 117, 230
AlwI GGATC 3 cut(s) 56, 69, 155
AlwNI CAGNNNCTG 1 cut(s) 877
Aor13HI TCCGGA 2 cut(s) 640, 762
AoxI GGCC 3 cut(s) 42, 604, 726
ApeKI GCWGC 4 cut(s) 25, 597, 719, 834
Asp718I GGTACC 1 cut(s) 199
AspLEI GCGC 4 cut(s) 628, 633, 750, 755
AspS9I GGNCC 2 cut(s) 189, 433
AsuHPI GGTGA 4 cut(s) 370, 583, 631, 869
AvaII GGWCC 2 cut(s) 189, 433
BaeGI GKGCMC 1 cut(s) 389
BaeI ACNNNNGTAYC 2 cut(s) 183, 216
BamHI GGATCC 1 cut(s) 61
BanI GGYRCC 2 cut(s) 199, 386
BbvI GCAGC 4 cut(s) 12, 584, 706, 821
BccI CCATC 3 cut(s) 428, 445, 449
BceAI ACGGC 2 cut(s) 639, 761
BcoDI GTCTC 2 cut(s) 117, 230
BfaI CTAG 1 cut(s) 566
BfuAI ACCTGC 1 cut(s) 845
BlpI GCTNAGC 1 cut(s) 341
Bme18I GGWCC 2 cut(s) 189, 433
BmgT120I GGNCC 2 cut(s) 189, 433
BmiI GGNNCC 5 cut(s) 63, 147, 201, 388, 460
BmrI ACTGGG 1 cut(s) 198
BmuI ACTGGG 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 132
Bpu10I CCTNAGC 1 cut(s) 366
Bpu1102I GCTNAGC 1 cut(s) 341
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 381
BsaWI WCCGGW 2 cut(s) 640, 762
BsaXI ACNNNNNCTCC 2 cut(s) 112, 142
Bsc4I CCNNNNNNNGG 6 cut(s) 232, 430, 647, 769, 820, 877
Bse118I RCCGGY 3 cut(s) 389, 691, 813
Bse1I ACTGG 3 cut(s) 204, 275, 882
Bse3DI GCAATG 1 cut(s) 335
BseAI TCCGGA 2 cut(s) 640, 762
BseDI CCNNGG 1 cut(s) 381
BseGI GGATG 3 cut(s) 420, 456, 460
BseLI CCNNNNNNNGG 6 cut(s) 232, 430, 647, 769, 820, 877
BseMI GCAATG 1 cut(s) 335
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 3 cut(s) 204, 275, 882
BseRI GAGGAG 4 cut(s) 248, 681, 803, 883
BseSI GKGCMC 1 cut(s) 389
BseXI GCAGC 4 cut(s) 12, 584, 706, 821
BsgI GTGCAG 1 cut(s) 729
Bsh1236I CGCG 4 cut(s) 628, 633, 750, 755
Bsh1285I CGRYCG 2 cut(s) 496, 679
BshFI GGCC 3 cut(s) 44, 606, 728
BshNI GGYRCC 2 cut(s) 199, 386
BsiEI CGRYCG 2 cut(s) 496, 679
BsiSI CCGG 7 cut(s) 390, 603, 641, 692, 725, 763, 814
BslI CCNNNNNNNGG 6 cut(s) 232, 430, 647, 769, 820, 877
BsmAI GTCTC 2 cut(s) 117, 230
BsmI GAATGC 1 cut(s) 331
BsnI GGCC 3 cut(s) 44, 606, 728
Bso31I GGTCTC 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 389
Bsp13I TCCGGA 2 cut(s) 640, 762
Bsp143I GATC 4 cut(s) 61, 160, 610, 732
Bsp1720I GCTNAGC 1 cut(s) 341
Bsp19I CCATGG 1 cut(s) 381
BspANI GGCC 3 cut(s) 44, 606, 728
BspCNI CTCAG 1 cut(s) 358
BspEI TCCGGA 2 cut(s) 640, 762
BspFNI CGCG 4 cut(s) 628, 633, 750, 755
BspLI GGNNCC 5 cut(s) 63, 147, 201, 388, 460
BspMI ACCTGC 1 cut(s) 845
BspPI GGATC 3 cut(s) 56, 69, 155
BspT107I GGYRCC 2 cut(s) 199, 386
BspTNI GGTCTC 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 335
BsrFI RCCGGY 3 cut(s) 389, 691, 813
BsrI ACTGG 3 cut(s) 204, 275, 882
BssAI RCCGGY 3 cut(s) 389, 691, 813
BssECI CCNNGG 1 cut(s) 381
BssMI GATC 4 cut(s) 61, 160, 610, 732
BssT1I CCWWGG 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 128
BstAPI GCANNNNNTGC 2 cut(s) 337, 716
BstC8I GCNNGC 3 cut(s) 485, 693, 815
BstDEI CTNAG 3 cut(s) 284, 341, 366
BstDSI CCRYGG 1 cut(s) 381
BstENI CCTNNNNNAGG 1 cut(s) 428
BstF5I GGATG 3 cut(s) 420, 456, 460
BstFNI CGCG 4 cut(s) 628, 633, 750, 755
BstHHI GCGC 4 cut(s) 628, 633, 750, 755
BstKTI GATC 4 cut(s) 64, 163, 613, 735
BstMAI GTCTC 2 cut(s) 117, 230
BstMBI GATC 4 cut(s) 61, 160, 610, 732
BstMCI CGRYCG 2 cut(s) 496, 679
BstMWI GCNNNNNNNGC 8 cut(s) 337, 480, 489, 603, 623, 716, 725, 745
BstSLI GKGCMC 1 cut(s) 389
BstUI CGCG 4 cut(s) 628, 633, 750, 755
BstV1I GCAGC 4 cut(s) 12, 584, 706, 821
BstX2I RGATCY 1 cut(s) 61
BstYI RGATCY 1 cut(s) 61
BsuRI GGCC 3 cut(s) 44, 606, 728
BtgI CCRYGG 1 cut(s) 381
BtsCI GGATG 3 cut(s) 420, 456, 460
BtsI GCAGTG 1 cut(s) 836
BtsIMutI CAGTG 2 cut(s) 211, 836
BveI ACCTGC 1 cut(s) 845
Cac8I GCNNGC 3 cut(s) 485, 693, 815
CaiI CAGNNNCTG 1 cut(s) 877
CfoI GCGC 4 cut(s) 628, 633, 750, 755
Cfr10I RCCGGY 3 cut(s) 389, 691, 813
Cfr13I GGNCC 2 cut(s) 189, 433
Csp6I GTAC 1 cut(s) 200
CviAII CATG 4 cut(s) 142, 252, 360, 382
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 3 cut(s) 284, 341, 366
DpnI GATC 4 cut(s) 63, 162, 612, 734
DpnII GATC 4 cut(s) 61, 160, 610, 732
Eco130I CCWWGG 1 cut(s) 381
Eco31I GGTCTC 1 cut(s) 230
Eco47I GGWCC 2 cut(s) 189, 433
Eco57I CTGAAG 1 cut(s) 413
EcoNI CCTNNNNNAGG 1 cut(s) 428
EcoT14I CCWWGG 1 cut(s) 381
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 4 cut(s) 145, 255, 363, 385
FatI CATG 4 cut(s) 141, 251, 359, 381
FokI GGATG 3 cut(s) 407, 463, 467
FspBI CTAG 1 cut(s) 566
GlaI GCGC 4 cut(s) 627, 632, 749, 754
GsuI CTGGAG 1 cut(s) 132
HaeIII GGCC 3 cut(s) 44, 606, 728
HapII CCGG 7 cut(s) 390, 603, 641, 692, 725, 763, 814
HhaI GCGC 4 cut(s) 628, 633, 750, 755
Hin1II CATG 4 cut(s) 145, 255, 363, 385
Hin6I GCGC 4 cut(s) 626, 631, 748, 753
HinP1I GCGC 4 cut(s) 626, 631, 748, 753
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 3 cut(s) 510, 686, 808
HpaII CCGG 7 cut(s) 390, 603, 641, 692, 725, 763, 814
HphI GGTGA 4 cut(s) 370, 583, 631, 869
Hpy166II GTNNAC 3 cut(s) 139, 290, 574
Hpy188I TCNGA 3 cut(s) 66, 468, 898
Hpy188III TCNNGA 6 cut(s) 149, 158, 260, 431, 641, 763
Hpy8I GTNNAC 3 cut(s) 139, 290, 574
Hpy99I CGWCG 2 cut(s) 747, 822
HpyAV CCTTC 1 cut(s) 25
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4IV ACGT 3 cut(s) 576, 592, 714
HpyCH4V TGCA 7 cut(s) 4, 186, 474, 710, 829, 837, 854
HpyF10VI GCNNNNNNNGC 8 cut(s) 337, 480, 489, 603, 623, 716, 725, 745
HpyF3I CTNAG 3 cut(s) 284, 341, 366
HpySE526I ACGT 3 cut(s) 576, 592, 714
Hsp92II CATG 4 cut(s) 145, 255, 363, 385
HspAI GCGC 4 cut(s) 626, 631, 748, 753
Kpn2I TCCGGA 2 cut(s) 640, 762
KpnI GGTACC 1 cut(s) 203
KroI GCCGGC 2 cut(s) 691, 813
KroNI GCCGGC 2 cut(s) 693, 815
Kzo9I GATC 4 cut(s) 61, 160, 610, 732
LmnI GCTCC 3 cut(s) 151, 605, 727
Lsp1109I GCAGC 4 cut(s) 12, 584, 706, 821
MaeI CTAG 1 cut(s) 566
MaeII ACGT 3 cut(s) 576, 592, 714
MaeIII GTNAC 2 cut(s) 206, 619
MalI GATC 4 cut(s) 63, 162, 612, 734
MboI GATC 4 cut(s) 61, 160, 610, 732
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 1 cut(s) 389
MluCI AATT 3 cut(s) 115, 279, 516
MlyI GAGTC 2 cut(s) 695, 817
Mph1103I ATGCAT 1 cut(s) 6
MreI CGCCGGCG 2 cut(s) 691, 813
MroI TCCGGA 2 cut(s) 640, 762
MroNI GCCGGC 2 cut(s) 691, 813
MseI TTAA 2 cut(s) 78, 96
MslI CAYNNNNRTG 2 cut(s) 9, 402
MspA1I CMGCKG 1 cut(s) 25
MspI CCGG 7 cut(s) 390, 603, 641, 692, 725, 763, 814
MteI GCGCNGCGC 2 cut(s) 629, 751
Mva1269I GAATGC 1 cut(s) 331
MvnI CGCG 4 cut(s) 628, 633, 750, 755
MwoI GCNNNNNNNGC 8 cut(s) 337, 480, 489, 603, 623, 716, 725, 745
NaeI GCCGGC 2 cut(s) 693, 815
NcoI CCATGG 1 cut(s) 381
NdeII GATC 4 cut(s) 61, 160, 610, 732
NgoMIV GCCGGC 2 cut(s) 691, 813
NlaIII CATG 4 cut(s) 145, 255, 363, 385
NlaIV GGNNCC 5 cut(s) 63, 147, 201, 388, 460
NmeAIII GCCGAG 1 cut(s) 70
NmuCI GTSAC 2 cut(s) 206, 619
NsiI ATGCAT 1 cut(s) 6
PaqCI CACCTGC 1 cut(s) 845
PctI GAATGC 1 cut(s) 331
PdiI GCCGGC 2 cut(s) 693, 815
PfeI GAWTC 1 cut(s) 510
PflMI CCANNNNNTGG 1 cut(s) 877
PleI GAGTC 2 cut(s) 694, 816
PpsI GAGTC 2 cut(s) 694, 816
PsiI TTATAA 1 cut(s) 323
Psp1406I AACGTT 1 cut(s) 576
PspN4I GGNNCC 5 cut(s) 63, 147, 201, 388, 460
PspPI GGNCC 2 cut(s) 189, 433
PstNI CAGNNNCTG 1 cut(s) 877
PsuI RGATCY 1 cut(s) 61
PvuII CAGCTG 1 cut(s) 25
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
RseI CAYNNNNRTG 2 cut(s) 9, 402
SaqAI TTAA 2 cut(s) 78, 96
Sau3AI GATC 4 cut(s) 61, 160, 610, 732
Sau96I GGNCC 2 cut(s) 189, 433
SchI GAGTC 2 cut(s) 695, 817
SduI GDGCHC 1 cut(s) 389
SgrAI CRCCGGYG 2 cut(s) 691, 813
SinI GGWCC 2 cut(s) 189, 433
SmiMI CAYNNNNRTG 2 cut(s) 9, 402
Sse9I AATT 3 cut(s) 115, 279, 516
SspMI CTAG 1 cut(s) 566
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 1 cut(s) 128
TaiI ACGT 3 cut(s) 579, 595, 717
TaqI TCGA 4 cut(s) 105, 259, 609, 731
TaqII GACCGA 1 cut(s) 254
TasI AATT 3 cut(s) 115, 279, 516
TauI GCSGC 6 cut(s) 250, 631, 636, 742, 753, 758
TfiI GAWTC 1 cut(s) 510
Tru1I TTAA 2 cut(s) 78, 96
Tru9I TTAA 2 cut(s) 78, 96
TscAI CASTG 2 cut(s) 211, 836
TseFI GTSAC 2 cut(s) 206, 619
TseI GCWGC 4 cut(s) 25, 597, 719, 834
Tsp45I GTSAC 2 cut(s) 206, 619
TspDTI ATGAA 1 cut(s) 324
TspRI CASTG 2 cut(s) 211, 836
Van91I CCANNNNNTGG 1 cut(s) 877
VpaK11BI GGWCC 2 cut(s) 189, 433
XagI CCTNNNNNAGG 1 cut(s) 428
XspI CTAG 1 cut(s) 566
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.