Rmu_sc0004944.1_g000004

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004944.1
N/A
Physical Location & Seq
Forward (+)
12835 .. 13296
462 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 462 bp
atggatcttgatattcttagttggaattgcagaggaatctgcaatgatgctacgatgagggcgctgaaggacttgtgtgctcagaatcacccacagattgtgtttttatgtgaaacaaagatcagcaagttggttgacttcgaaagcctacgtcgtgcgttaggttttgctcatgtagaggaggttttgagtgctggccaagccgatggtttggggcttttctggagcgatgatgtaaagattcaaattggtaccaaatcggcacatcacattgatgtagtggttgtgggggatactggggctccttcatggtgtctgatgggattttatgggtatgcccgtactggagagagagataagtcatggcaattgctgcgtgatctttccgacatggattctctgccttgggtggtgttaggggattttaatgagatcctgaatagcggggagaatatttcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

153

Amino Acids

17.01

Weight (kDa)

4.71

Isoelectric Point (pI)

25.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 6 - 150 3.4e-14 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 251
AciI CCGC 1 cut(s) 444
AclWI GGATC 2 cut(s) 12, 427
AcoI YGGCCR 1 cut(s) 196
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 2 cut(s) 253, 343
AgsI TTSAA 1 cut(s) 245
Alw21I GWGCWC 1 cut(s) 82
AlwI GGATC 2 cut(s) 12, 427
AoxI GGCC 1 cut(s) 196
ApeKI GCWGC 1 cut(s) 373
Asp718I GGTACC 1 cut(s) 251
AspLEI GCGC 1 cut(s) 64
AsuHPI GGTGA 1 cut(s) 80
AsuII TTCGAA 1 cut(s) 141
BalI TGGCCA 1 cut(s) 198
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 1 cut(s) 304
Bbv12I GWGCWC 1 cut(s) 82
BbvI GCAGC 1 cut(s) 360
BccI CCATC 2 cut(s) 200, 313
BciVI GTATCC 1 cut(s) 286
BfoI RGCGCY 1 cut(s) 65
BfuI GTATCC 1 cut(s) 286
BisI GCNGC 1 cut(s) 374
BlsI GCNGC 1 cut(s) 375
BmiI GGNNCC 2 cut(s) 253, 303
BmrI ACTGGG 1 cut(s) 306
BmsI GCATC 1 cut(s) 37
BmuI ACTGGG 1 cut(s) 306
BpmI CTGGAG 2 cut(s) 244, 366
Bpu14I TTCGAA 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 404
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bse1I ACTGG 2 cut(s) 301, 349
Bse3DI GCAATG 1 cut(s) 49
BseDI CCNNGG 1 cut(s) 404
BseMI GCAATG 1 cut(s) 49
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 2 cut(s) 301, 349
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 1 cut(s) 360
BshFI GGCC 1 cut(s) 198
BshNI GGYRCC 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 82
BsnI GGCC 1 cut(s) 198
Bsp119I TTCGAA 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 82, 304
Bsp143I GATC 4 cut(s) 4, 120, 379, 432
BspACI CCGC 1 cut(s) 444
BspANI GGCC 1 cut(s) 198
BspCNI CTCAG 1 cut(s) 94
BspLI GGNNCC 2 cut(s) 253, 303
BspPI GGATC 2 cut(s) 12, 427
BspT104I TTCGAA 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 49
BsrI ACTGG 2 cut(s) 301, 349
BssECI CCNNGG 1 cut(s) 404
BssMI GATC 4 cut(s) 4, 120, 379, 432
BssT1I CCWWGG 1 cut(s) 404
BstAPI GCANNNNNTGC 1 cut(s) 373
BstBI TTCGAA 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 2 cut(s) 17, 81
BstH2I RGCGCY 1 cut(s) 65
BstHHI GCGC 1 cut(s) 64
BstKTI GATC 4 cut(s) 7, 123, 382, 435
BstMBI GATC 4 cut(s) 4, 120, 379, 432
BstMWI GCNNNNNNNGC 2 cut(s) 200, 373
BstV1I GCAGC 1 cut(s) 360
BstX2I RGATCY 2 cut(s) 4, 432
BstXI CCANNNNNNTGG 1 cut(s) 206
BstYI RGATCY 2 cut(s) 4, 432
BsuI GTATCC 1 cut(s) 286
BsuRI GGCC 1 cut(s) 198
BtgZI GCGATG 1 cut(s) 243
Cac8I GCNNGC 1 cut(s) 196
CfoI GCGC 1 cut(s) 64
Csp6I GTAC 2 cut(s) 252, 342
CviAII CATG 4 cut(s) 173, 309, 363, 391
CviJI RGCY 5 cut(s) 147, 198, 203, 217, 302
CviKI_1 RGCY 5 cut(s) 147, 198, 203, 217, 302
CviQI GTAC 2 cut(s) 252, 342
DdeI CTNAG 2 cut(s) 17, 81
DpnI GATC 4 cut(s) 6, 122, 381, 434
DpnII GATC 4 cut(s) 4, 120, 379, 432
EaeI YGGCCR 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 86
EcoT14I CCWWGG 1 cut(s) 404
EcoT38I GRGCYC 1 cut(s) 304
ErhI CCWWGG 1 cut(s) 404
FaeI CATG 4 cut(s) 176, 312, 366, 394
FaiI YATR 7 cut(s) 109, 174, 310, 330, 336, 364, 392
FatI CATG 4 cut(s) 172, 308, 362, 390
FauI CCCGC 1 cut(s) 437
Fnu4HI GCNGC 1 cut(s) 374
FriOI GRGCYC 1 cut(s) 304
Fsp4HI GCNGC 1 cut(s) 374
GlaI GCGC 1 cut(s) 63
GluI GCNGC 1 cut(s) 374
GsuI CTGGAG 2 cut(s) 244, 366
HaeII RGCGCY 1 cut(s) 65
HaeIII GGCC 1 cut(s) 198
HhaI GCGC 1 cut(s) 64
Hin1II CATG 4 cut(s) 176, 312, 366, 394
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 4 cut(s) 36, 85, 241, 395
HphI GGTGA 1 cut(s) 80
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 3 cut(s) 84, 318, 388
Hpy188III TCNNGA 4 cut(s) 8, 223, 436, 459
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 1 cut(s) 156
HpyAV CCTTC 2 cut(s) 61, 315
HpyCH4IV ACGT 1 cut(s) 151
HpyCH4V TGCA 2 cut(s) 30, 42
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 373
HpyF3I CTNAG 2 cut(s) 17, 81
HpySE526I ACGT 1 cut(s) 151
Hsp92II CATG 4 cut(s) 176, 312, 366, 394
HspAI GCGC 1 cut(s) 62
KpnI GGTACC 1 cut(s) 255
Kzo9I GATC 4 cut(s) 4, 120, 379, 432
LmnI GCTCC 2 cut(s) 225, 307
LpnPI CCDG 5 cut(s) 180, 208, 282, 330, 449
Lsp1109I GCAGC 1 cut(s) 360
LweI GCATC 1 cut(s) 37
MaeII ACGT 1 cut(s) 151
MalI GATC 4 cut(s) 6, 122, 381, 434
MboI GATC 4 cut(s) 4, 120, 379, 432
MfeI CAATTG 1 cut(s) 368
MflI RGATCY 2 cut(s) 4, 432
MhlI GDGCHC 2 cut(s) 82, 304
MlsI TGGCCA 1 cut(s) 198
MluCI AATT 3 cut(s) 25, 246, 368
MluNI TGGCCA 1 cut(s) 198
MmeI TCCRAC 1 cut(s) 411
MnlI CCTC 4 cut(s) 26, 51, 172, 175
Mox20I TGGCCA 1 cut(s) 198
MscI TGGCCA 1 cut(s) 198
MseI TTAA 1 cut(s) 426
MslI CAYNNNNRTG 1 cut(s) 273
Msp20I TGGCCA 1 cut(s) 198
MunI CAATTG 1 cut(s) 368
MwoI GCNNNNNNNGC 2 cut(s) 200, 373
NdeII GATC 4 cut(s) 4, 120, 379, 432
NlaIII CATG 4 cut(s) 176, 312, 366, 394
NlaIV GGNNCC 2 cut(s) 253, 303
NspV TTCGAA 1 cut(s) 141
PfeI GAWTC 4 cut(s) 36, 85, 241, 395
PkrI GCNGC 1 cut(s) 375
PspN4I GGNNCC 2 cut(s) 253, 303
PsuI RGATCY 2 cut(s) 4, 432
RsaI GTAC 2 cut(s) 253, 343
RsaNI GTAC 2 cut(s) 252, 342
RseI CAYNNNNRTG 1 cut(s) 273
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 1 cut(s) 374
Sau3AI GATC 4 cut(s) 4, 120, 379, 432
SduI GDGCHC 2 cut(s) 82, 304
SetI ASST 3 cut(s) 154, 166, 186
SfaNI GCATC 1 cut(s) 37
SfuI TTCGAA 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 273
Sse9I AATT 3 cut(s) 25, 246, 368
SsiI CCGC 1 cut(s) 444
SspI AATATT 1 cut(s) 454
StyI CCWWGG 1 cut(s) 404
TaiI ACGT 1 cut(s) 154
TaqI TCGA 1 cut(s) 141
TasI AATT 3 cut(s) 25, 246, 368
TfiI GAWTC 4 cut(s) 36, 85, 241, 395
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TseI GCWGC 1 cut(s) 373
TspDTI ATGAA 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.