Rmu_sc0005129.1_g000018

APO protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005129.1
N/A
Physical Location & Seq
Reverse (-)
56337 .. 58851
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 831 bp
atgcagtttgagcatggaaagcttcgtaaagggggaagtacatgcagaaccatctactctgtcagtaggaagcccagtgtagaaccaactttcaggaagcgagatgcatctccccaaaatgaggatcttccagctgtactacctaagcaaaagaaaaaaccatatcccattcccttaaagaagattaagcaagctccaaaggtgaacaaaaaacttgcagaaaagggtatagaaaagccgctcaaccctccaaaaaatggattacttgaccctgatctaattcctgttgctcatcaagtactgcatgcctggaaagctttgattaaaggccttgcacagctgttgcatgttattctagtatatggttgcctcgattggattgatggggttttgttctttattgattataccatagtaatgcaaaccaatgataattctaatacagatcgatgtggtgatagttttacgatgtgtatattctgttttcctacttcctcagtcattccacttcatgatcaccctggcatgactgtttttagcaaagttctgtttggttctttgcatgtgacaacttatgattgggtggagccttcccacgaaagcaagggatcaaattactttccaggagatgagatgactcgaattttctggaaatcaatcaaggataagcttattctcccctttttggatttggtcattaagtactttgaccttggtcttcctcaccgtgataccactgatgataaagttaccgttgaaagtgcaaaggctactcttaagaggattagaatggggctgctccagctagaagtattccggtgtggatcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

276

Amino Acids

31.29

Weight (kDa)

9.24

Isoelectric Point (pI)

25.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 30 - 124 4.9e-35 APO RNA-binding
PCO_ADO PF07847 153 - 204 1.1e-23 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 257
AccBSI CCGCTC 1 cut(s) 241
AciI CCGC 1 cut(s) 239
AclWI GGATC 3 cut(s) 132, 616, 819
AcsI RAATTY 1 cut(s) 642
AfaI GTAC 4 cut(s) 40, 138, 300, 704
AfiI CCNNNNNNNGG 3 cut(s) 121, 257, 685
AflII CTTAAG 1 cut(s) 776
AgsI TTSAA 1 cut(s) 758
AjnI CCWGG 3 cut(s) 308, 520, 622
AluBI AGCT 7 cut(s) 22, 134, 194, 317, 340, 670, 805
AluI AGCT 7 cut(s) 22, 134, 194, 317, 340, 670, 805
AlwI GGATC 3 cut(s) 132, 616, 819
AoxI GGCC 1 cut(s) 328
ApeKI GCWGC 1 cut(s) 796
ApoI RAATTY 1 cut(s) 642
AsuHPI GGTGA 4 cut(s) 214, 467, 509, 716
BamHI GGATCC 1 cut(s) 824
BbsI GAAGAC 1 cut(s) 710
BbvI GCAGC 1 cut(s) 783
BccI CCATC 2 cut(s) 59, 377
BciT130I CCWGG 3 cut(s) 310, 522, 624
BclI TGATCA 1 cut(s) 514
BfaI CTAG 2 cut(s) 356, 806
BfrI CTTAAG 1 cut(s) 776
BisI GCNGC 2 cut(s) 239, 797
BlsI GCNGC 2 cut(s) 240, 798
BmcAI AGTACT 2 cut(s) 300, 704
Bme1390I CCNGG 3 cut(s) 310, 522, 624
BmiI GGNNCC 2 cut(s) 588, 826
BmrFI CCNGG 3 cut(s) 310, 522, 624
BmrI ACTGGG 1 cut(s) 69
BmsI GCATC 2 cut(s) 94, 116
BmuI ACTGGG 1 cut(s) 69
BoxI GACNNNNGTC 1 cut(s) 714
BpiI GAAGAC 1 cut(s) 710
BpmI CTGGAG 1 cut(s) 785
Bpu10I CCTNAGC 1 cut(s) 144
Bsa29I ATCGAT 1 cut(s) 448
BsaJI CCNNGG 2 cut(s) 520, 712
BsaWI WCCGGW 1 cut(s) 816
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 257, 685
Bse1I ACTGG 1 cut(s) 75
BseBI CCWGG 3 cut(s) 310, 522, 624
BseCI ATCGAT 1 cut(s) 448
BseDI CCNNGG 2 cut(s) 520, 712
BseLI CCNNNNNNNGG 3 cut(s) 121, 257, 685
BseMII CTCAG 1 cut(s) 510
BseNI ACTGG 1 cut(s) 75
BseXI GCAGC 1 cut(s) 783
BshFI GGCC 1 cut(s) 330
BshVI ATCGAT 1 cut(s) 448
BsiSI CCGG 1 cut(s) 817
BslI CCNNNNNNNGG 3 cut(s) 121, 257, 685
BsnI GGCC 1 cut(s) 330
Bsp143I GATC 6 cut(s) 124, 274, 445, 514, 608, 824
BspACI CCGC 1 cut(s) 239
BspANI GGCC 1 cut(s) 330
BspCNI CTCAG 1 cut(s) 509
BspDI ATCGAT 1 cut(s) 448
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 2 cut(s) 588, 826
BspPI GGATC 3 cut(s) 132, 616, 819
BspTI CTTAAG 1 cut(s) 776
BsrBI CCGCTC 1 cut(s) 241
BsrI ACTGG 1 cut(s) 75
BssECI CCNNGG 2 cut(s) 520, 712
BssMI GATC 6 cut(s) 124, 274, 445, 514, 608, 824
BssT1I CCWWGG 1 cut(s) 712
Bst2UI CCWGG 3 cut(s) 310, 522, 624
Bst4CI ACNGT 3 cut(s) 532, 728, 754
BstAFI CTTAAG 1 cut(s) 776
BstC8I GCNNGC 2 cut(s) 192, 306
BstDEI CTNAG 2 cut(s) 144, 496
BstKTI GATC 6 cut(s) 127, 277, 448, 517, 611, 827
BstMBI GATC 6 cut(s) 124, 274, 445, 514, 608, 824
BstMWI GCNNNNNNNGC 4 cut(s) 10, 19, 314, 802
BstNI CCWGG 3 cut(s) 310, 522, 624
BstNSI RCATGY 4 cut(s) 45, 308, 350, 566
BstPAI GACNNNNGTC 1 cut(s) 714
BstSCI CCNGG 3 cut(s) 308, 520, 622
BstV1I GCAGC 1 cut(s) 783
BstV2I GAAGAC 1 cut(s) 710
BstX2I RGATCY 2 cut(s) 124, 824
BstYI RGATCY 2 cut(s) 124, 824
Bsu15I ATCGAT 1 cut(s) 448
BsuRI GGCC 1 cut(s) 330
BsuTUI ATCGAT 1 cut(s) 448
BtsIMutI CAGTG 2 cut(s) 82, 735
Cac8I GCNNGC 2 cut(s) 192, 306
CciI TCATGA 1 cut(s) 511
ClaI ATCGAT 1 cut(s) 448
Csp6I GTAC 4 cut(s) 39, 137, 299, 703
CviAII CATG 7 cut(s) 14, 42, 305, 347, 512, 526, 563
CviQI GTAC 4 cut(s) 39, 137, 299, 703
DdeI CTNAG 2 cut(s) 144, 496
DpnI GATC 6 cut(s) 126, 276, 447, 516, 610, 826
DpnII GATC 6 cut(s) 124, 274, 445, 514, 608, 824
Eco130I CCWWGG 1 cut(s) 712
Eco147I AGGCCT 1 cut(s) 330
EcoRII CCWGG 3 cut(s) 308, 520, 622
EcoT14I CCWWGG 1 cut(s) 712
EcoT22I ATGCAT 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 712
FaeI CATG 7 cut(s) 17, 45, 308, 350, 515, 529, 566
FatI CATG 7 cut(s) 13, 41, 304, 346, 511, 525, 562
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 2 cut(s) 239, 797
Fsp4HI GCNGC 2 cut(s) 239, 797
FspBI CTAG 2 cut(s) 356, 806
GluI GCNGC 2 cut(s) 239, 797
GsuI CTGGAG 1 cut(s) 785
HaeIII GGCC 1 cut(s) 330
HapII CCGG 1 cut(s) 817
Hin1II CATG 7 cut(s) 17, 45, 308, 350, 515, 529, 566
HindIII AAGCTT 3 cut(s) 20, 315, 668
HinfI GANTC 1 cut(s) 637
HpaII CCGG 1 cut(s) 817
HphI GGTGA 4 cut(s) 214, 467, 509, 716
Hpy166II GTNNAC 1 cut(s) 205
Hpy188III TCNNGA 3 cut(s) 94, 512, 649
Hpy8I GTNNAC 1 cut(s) 205
HpyAV CCTTC 1 cut(s) 600
HpyCH4III ACNGT 3 cut(s) 532, 728, 754
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 19, 314, 802
HpyF3I CTNAG 2 cut(s) 144, 496
Hsp92II CATG 7 cut(s) 17, 45, 308, 350, 515, 529, 566
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 6 cut(s) 124, 274, 445, 514, 608, 824
LmnI GCTCC 3 cut(s) 199, 586, 804
Lsp1109I GCAGC 1 cut(s) 783
LweI GCATC 2 cut(s) 94, 116
MaeI CTAG 2 cut(s) 356, 806
MaeIII GTNAC 2 cut(s) 565, 748
MalI GATC 6 cut(s) 126, 276, 447, 516, 610, 826
MbiI CCGCTC 1 cut(s) 241
MboI GATC 6 cut(s) 124, 274, 445, 514, 608, 824
MboII GAAGA 3 cut(s) 119, 193, 710
MflI RGATCY 2 cut(s) 124, 824
MluCI AATT 4 cut(s) 279, 433, 613, 642
MlyI GAGTC 1 cut(s) 631
MnlI CCTC 6 cut(s) 115, 258, 380, 505, 732, 774
Mph1103I ATGCAT 1 cut(s) 109
MseI TTAA 5 cut(s) 176, 186, 324, 699, 777
MslI CAYNNNNRTG 1 cut(s) 416
MspA1I CMGCKG 2 cut(s) 134, 340
MspCI CTTAAG 1 cut(s) 776
MspI CCGG 1 cut(s) 817
MspR9I CCNGG 3 cut(s) 310, 522, 624
MvaI CCWGG 3 cut(s) 310, 522, 624
MwoI GCNNNNNNNGC 4 cut(s) 10, 19, 314, 802
NdeII GATC 6 cut(s) 124, 274, 445, 514, 608, 824
NlaIII CATG 7 cut(s) 17, 45, 308, 350, 515, 529, 566
NlaIV GGNNCC 2 cut(s) 588, 826
NmuCI GTSAC 1 cut(s) 565
NsiI ATGCAT 1 cut(s) 109
NspI RCATGY 4 cut(s) 45, 308, 350, 566
PaeI GCATGC 1 cut(s) 308
PagI TCATGA 1 cut(s) 511
PceI AGGCCT 1 cut(s) 330
PflMI CCANNNNNTGG 1 cut(s) 257
PfoI TCCNGGA 1 cut(s) 622
PkrI GCNGC 2 cut(s) 240, 798
PleI GAGTC 1 cut(s) 631
PpsI GAGTC 1 cut(s) 631
PshAI GACNNNNGTC 1 cut(s) 714
Psp6I CCWGG 3 cut(s) 308, 520, 622
PspGI CCWGG 3 cut(s) 308, 520, 622
PspN4I GGNNCC 2 cut(s) 588, 826
PsuI RGATCY 2 cut(s) 124, 824
PvuII CAGCTG 2 cut(s) 134, 340
RsaI GTAC 4 cut(s) 40, 138, 300, 704
RsaNI GTAC 4 cut(s) 39, 137, 299, 703
RseI CAYNNNNRTG 1 cut(s) 416
SaqAI TTAA 5 cut(s) 176, 186, 324, 699, 777
SatI GCNGC 2 cut(s) 239, 797
Sau3AI GATC 6 cut(s) 124, 274, 445, 514, 608, 824
ScaI AGTACT 2 cut(s) 300, 704
SchI GAGTC 1 cut(s) 631
ScrFI CCNGG 3 cut(s) 310, 522, 624
SfaNI GCATC 2 cut(s) 94, 116
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 1 cut(s) 776
SmoI CTYRAG 1 cut(s) 776
SphI GCATGC 1 cut(s) 308
Sse9I AATT 4 cut(s) 279, 433, 613, 642
SseBI AGGCCT 1 cut(s) 330
SsiI CCGC 1 cut(s) 239
SspMI CTAG 2 cut(s) 356, 806
StuI AGGCCT 1 cut(s) 330
StyD4I CCNGG 3 cut(s) 308, 520, 622
StyI CCWWGG 1 cut(s) 712
TaaI ACNGT 3 cut(s) 532, 728, 754
TaqI TCGA 3 cut(s) 372, 448, 640
TasI AATT 4 cut(s) 279, 433, 613, 642
TatI WGTACW 4 cut(s) 38, 136, 298, 702
TauI GCSGC 1 cut(s) 241
Tru1I TTAA 5 cut(s) 176, 186, 324, 699, 777
Tru9I TTAA 5 cut(s) 176, 186, 324, 699, 777
TscAI CASTG 2 cut(s) 82, 742
TseFI GTSAC 1 cut(s) 565
TseI GCWGC 1 cut(s) 796
Tsp45I GTSAC 1 cut(s) 565
TspDTI ATGAA 1 cut(s) 500
TspRI CASTG 2 cut(s) 82, 742
Van91I CCANNNNNTGG 1 cut(s) 257
Vha464I CTTAAG 1 cut(s) 776
XapI RAATTY 1 cut(s) 642
XceI RCATGY 4 cut(s) 45, 308, 350, 566
XspI CTAG 2 cut(s) 356, 806
ZrmI AGTACT 2 cut(s) 300, 704
Zsp2I ATGCAT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.