Rmu_sc0005137.1_g000008

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005137.1
N/A
Physical Location & Seq
Reverse (-)
21254 .. 22246
993 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 720 bp
ctgcagcccaaaaagttgagcccacgtgcagcaagccgccaagctgccaagcccaagccgccaagtctctctgccacgttagcactggacctgccacgtcagcactttggtcaacggtcagtcaacgcggtcaacacttttctggccactttccggctacttttccggccaacttctgtccacttatccgtcagctacagtacttttccggcatctacagtacttttcctgccactttcatccatcagctacagtagcttccggcgacattccggcaactacagtacttccggcgacatttttccggccaacttttcggtcaagatttccgacatcttttcaagaaatttaatagtttttcgtggtagcccaaaacaagctaaagccataattctcatgataaggcacatgaatgacgcgctccaaaatgaatacctcaatgaggaagacccaagaaggctatgggtagaacttgagcagcgctttggcaacgttcgtgactccctgcttcctgatttagaagtgcgatggcatagcctccgcttttgtgatttcaagtctgtgcttgattataactcgggagctcttcgtatcaagtctctgatggagttttgtggccaagccataactgatacgatgttgatcgagaagactctctccaccttccccgtctttgcactgatgatttcaaagaattatcggattgatgtaaatgcataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

239

Amino Acids

27.14

Weight (kDa)

9.95

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
Acc36I ACCTGC 1 cut(s) 99
AccII CGCG 2 cut(s) 128, 419
AciI CCGC 4 cut(s) 37, 59, 128, 541
AclI AACGTT 1 cut(s) 492
AcoI YGGCCR 4 cut(s) 144, 167, 306, 616
AcsI RAATTY 1 cut(s) 346
AcvI CACGTG 1 cut(s) 26
AfaI GTAC 3 cut(s) 202, 222, 286
AfeI AGCGCT 1 cut(s) 482
AfiI CCNNNNNNNGG 2 cut(s) 153, 442
AgsI TTSAA 3 cut(s) 342, 556, 690
AjiI CACGTC 1 cut(s) 98
AluBI AGCT 6 cut(s) 44, 195, 249, 258, 380, 584
AluI AGCT 6 cut(s) 44, 195, 249, 258, 380, 584
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 2 cut(s) 71, 603
Ama87I CYCGRG 1 cut(s) 577
Aor51HI AGCGCT 1 cut(s) 482
AoxI GGCC 4 cut(s) 144, 167, 306, 616
ApeKI GCWGC 4 cut(s) 4, 29, 44, 478
ApoI RAATTY 1 cut(s) 346
AspLEI GCGC 2 cut(s) 421, 483
AspS9I GGNCC 1 cut(s) 88
AvaI CYCGRG 1 cut(s) 577
AvaII GGWCC 1 cut(s) 88
BalI TGGCCA 2 cut(s) 146, 618
BanII GRGCYC 2 cut(s) 23, 586
BbrPI CACGTG 1 cut(s) 26
BbsI GAAGAC 2 cut(s) 453, 656
Bbv12I GWGCWC 1 cut(s) 586
BbvI GCAGC 4 cut(s) 16, 31, 41, 490
BccI CCATC 3 cut(s) 251, 522, 598
BcoDI GTCTC 2 cut(s) 71, 603
BfmI CTRYAG 5 cut(s) 2, 196, 216, 250, 280
BfoI RGCGCY 1 cut(s) 484
BfuAI ACCTGC 1 cut(s) 99
BisI GCNGC 6 cut(s) 5, 30, 37, 45, 59, 479
BlsI GCNGC 6 cut(s) 6, 31, 38, 46, 60, 480
BmcAI AGTACT 3 cut(s) 202, 222, 286
Bme18I GGWCC 1 cut(s) 88
BmeT110I CYCGRG 1 cut(s) 577
BmgBI CACGTC 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 88
BmsI GCATC 1 cut(s) 221
BpiI GAAGAC 2 cut(s) 453, 656
BpuEI CTTGAG 1 cut(s) 494
BsaAI YACGTR 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 641
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 442
Bse1I ACTGG 1 cut(s) 90
Bse8I GATNNNNATC 1 cut(s) 641
BseGI GGATG 1 cut(s) 239
BseJI GATNNNNATC 1 cut(s) 641
BseLI CCNNNNNNNGG 2 cut(s) 153, 442
BseNI ACTGG 1 cut(s) 90
BseXI GCAGC 4 cut(s) 16, 31, 41, 490
BsgI GTGCAG 1 cut(s) 48
Bsh1236I CGCG 2 cut(s) 128, 419
BshFI GGCC 4 cut(s) 146, 169, 308, 618
BsiHKAI GWGCWC 1 cut(s) 586
BsiHKCI CYCGRG 1 cut(s) 577
BsiSI CCGG 7 cut(s) 154, 166, 209, 262, 273, 291, 305
BslI CCNNNNNNNGG 2 cut(s) 153, 442
BsmAI GTCTC 2 cut(s) 71, 603
BsnI GGCC 4 cut(s) 146, 169, 308, 618
BsoBI CYCGRG 1 cut(s) 577
Bsp1286I GDGCHC 2 cut(s) 23, 586
Bsp143I GATC 1 cut(s) 642
BspACI CCGC 4 cut(s) 37, 59, 128, 541
BspANI GGCC 4 cut(s) 146, 169, 308, 618
BspFNI CGCG 2 cut(s) 128, 419
BspHI TCATGA 1 cut(s) 396
BspMAI CTGCAG 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 99
BspQI GCTCTTC 1 cut(s) 591
BsrI ACTGG 1 cut(s) 90
BssMI GATC 1 cut(s) 642
Bst4CI ACNGT 5 cut(s) 117, 200, 220, 254, 284
Bst6I CTCTTC 1 cut(s) 591
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 34
BstENI CCTNNNNNAGG 1 cut(s) 440
BstF5I GGATG 1 cut(s) 239
BstFNI CGCG 2 cut(s) 128, 419
BstH2I RGCGCY 1 cut(s) 484
BstHHI GCGC 2 cut(s) 421, 483
BstKTI GATC 1 cut(s) 645
BstMAI GTCTC 2 cut(s) 71, 603
BstMBI GATC 1 cut(s) 642
BstMWI GCNNNNNNNGC 4 cut(s) 58, 80, 100, 255
BstSFI CTRYAG 5 cut(s) 2, 196, 216, 250, 280
BstUI CGCG 2 cut(s) 128, 419
BstV1I GCAGC 4 cut(s) 16, 31, 41, 490
BstV2I GAAGAC 2 cut(s) 453, 656
BsuRI GGCC 4 cut(s) 146, 169, 308, 618
BtgZI GCGATG 1 cut(s) 541
BtrI CACGTC 1 cut(s) 98
BtsCI GGATG 1 cut(s) 239
BtsIMutI CAGTG 2 cut(s) 83, 677
BveI ACCTGC 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 34
CciI TCATGA 1 cut(s) 396
CfoI GCGC 2 cut(s) 421, 483
Cfr13I GGNCC 1 cut(s) 88
CseI GACGC 1 cut(s) 425
Csp6I GTAC 3 cut(s) 201, 221, 285
CviAII CATG 2 cut(s) 397, 409
CviQI GTAC 3 cut(s) 201, 221, 285
DpnI GATC 1 cut(s) 644
DpnII GATC 1 cut(s) 642
EaeI YGGCCR 4 cut(s) 144, 167, 306, 616
Eam1104I CTCTTC 1 cut(s) 591
EarI CTCTTC 1 cut(s) 591
Ecl136II GAGCTC 1 cut(s) 584
Eco24I GRGCYC 2 cut(s) 23, 586
Eco47I GGWCC 1 cut(s) 88
Eco47III AGCGCT 1 cut(s) 482
Eco53kI GAGCTC 1 cut(s) 584
Eco72I CACGTG 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 577
EcoICRI GAGCTC 1 cut(s) 584
EcoNI CCTNNNNNAGG 1 cut(s) 440
EcoT22I ATGCAT 1 cut(s) 718
EcoT38I GRGCYC 2 cut(s) 23, 586
FaeI CATG 2 cut(s) 400, 412
FaiI YATR 8 cut(s) 389, 398, 410, 463, 534, 573, 626, 718
FatI CATG 2 cut(s) 396, 408
Fnu4HI GCNGC 6 cut(s) 5, 30, 37, 45, 59, 479
FokI GGATG 1 cut(s) 226
FriOI GRGCYC 2 cut(s) 23, 586
Fsp4HI GCNGC 6 cut(s) 5, 30, 37, 45, 59, 479
GlaI GCGC 2 cut(s) 420, 482
GluI GCNGC 6 cut(s) 5, 30, 37, 45, 59, 479
HaeII RGCGCY 1 cut(s) 484
HaeIII GGCC 4 cut(s) 146, 169, 308, 618
HapII CCGG 7 cut(s) 154, 166, 209, 262, 273, 291, 305
HgaI GACGC 1 cut(s) 425
HhaI GCGC 2 cut(s) 421, 483
Hin1II CATG 2 cut(s) 400, 412
Hin6I GCGC 2 cut(s) 419, 481
HinP1I GCGC 2 cut(s) 419, 481
HincII GTYRAC 3 cut(s) 113, 124, 133
HindII GTYRAC 3 cut(s) 113, 124, 133
HinfI GANTC 2 cut(s) 500, 652
HpaII CCGG 7 cut(s) 154, 166, 209, 262, 273, 291, 305
Hpy166II GTNNAC 4 cut(s) 113, 124, 133, 181
Hpy188I TCNGA 3 cut(s) 331, 603, 702
Hpy188III TCNNGA 7 cut(s) 322, 342, 397, 497, 512, 579, 646
Hpy8I GTNNAC 4 cut(s) 113, 124, 133, 181
HpyAV CCTTC 2 cut(s) 450, 673
HpyCH4III ACNGT 5 cut(s) 117, 200, 220, 254, 284
HpyCH4IV ACGT 4 cut(s) 25, 77, 97, 492
HpyCH4V TGCA 4 cut(s) 4, 29, 677, 716
HpyF10VI GCNNNNNNNGC 4 cut(s) 58, 80, 100, 255
HpySE526I ACGT 4 cut(s) 25, 77, 97, 492
Hsp92II CATG 2 cut(s) 400, 412
HspAI GCGC 2 cut(s) 419, 481
Kzo9I GATC 1 cut(s) 642
LguI GCTCTTC 1 cut(s) 591
LmnI GCTCC 2 cut(s) 426, 581
Lsp1109I GCAGC 4 cut(s) 16, 31, 41, 490
LweI GCATC 1 cut(s) 221
MaeII ACGT 4 cut(s) 25, 77, 97, 492
MaeIII GTNAC 1 cut(s) 497
MalI GATC 1 cut(s) 644
MboI GATC 1 cut(s) 642
MboII GAAGA 3 cut(s) 458, 578, 661
MhlI GDGCHC 2 cut(s) 23, 586
MlsI TGGCCA 2 cut(s) 146, 618
MluCI AATT 3 cut(s) 346, 390, 694
MluNI TGGCCA 2 cut(s) 146, 618
MlyI GAGTC 2 cut(s) 494, 646
MmeI TCCRAC 1 cut(s) 354
MnlI CCTC 3 cut(s) 436, 446, 548
Mox20I TGGCCA 2 cut(s) 146, 618
Mph1103I ATGCAT 1 cut(s) 718
MscI TGGCCA 2 cut(s) 146, 618
MseI TTAA 1 cut(s) 350
MslI CAYNNNNRTG 1 cut(s) 411
Msp20I TGGCCA 2 cut(s) 146, 618
MspI CCGG 7 cut(s) 154, 166, 209, 262, 273, 291, 305
MvnI CGCG 2 cut(s) 128, 419
MwoI GCNNNNNNNGC 4 cut(s) 58, 80, 100, 255
NdeII GATC 1 cut(s) 642
NlaIII CATG 2 cut(s) 400, 412
NmuCI GTSAC 1 cut(s) 497
NsiI ATGCAT 1 cut(s) 718
PagI TCATGA 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 591
PkrI GCNGC 6 cut(s) 6, 31, 38, 46, 60, 480
PleI GAGTC 2 cut(s) 494, 646
PmaCI CACGTG 1 cut(s) 26
PmlI CACGTG 1 cut(s) 26
PpsI GAGTC 2 cut(s) 494, 646
Ppu21I YACGTR 1 cut(s) 26
PsiI TTATAA 1 cut(s) 573
Psp124BI GAGCTC 1 cut(s) 586
Psp1406I AACGTT 1 cut(s) 492
PspCI CACGTG 1 cut(s) 26
PspPI GGNCC 1 cut(s) 88
PstI CTGCAG 1 cut(s) 6
RsaI GTAC 3 cut(s) 202, 222, 286
RsaNI GTAC 3 cut(s) 201, 221, 285
RseI CAYNNNNRTG 1 cut(s) 411
SacI GAGCTC 1 cut(s) 586
SapI GCTCTTC 1 cut(s) 591
SaqAI TTAA 1 cut(s) 350
SatI GCNGC 6 cut(s) 5, 30, 37, 45, 59, 479
Sau3AI GATC 1 cut(s) 642
Sau96I GGNCC 1 cut(s) 88
ScaI AGTACT 3 cut(s) 202, 222, 286
SchI GAGTC 2 cut(s) 494, 646
SduI GDGCHC 2 cut(s) 23, 586
SfaNI GCATC 1 cut(s) 221
SfcI CTRYAG 5 cut(s) 2, 196, 216, 250, 280
SinI GGWCC 1 cut(s) 88
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 3 cut(s) 346, 390, 694
SsiI CCGC 4 cut(s) 37, 59, 128, 541
SstI GAGCTC 1 cut(s) 586
TaaI ACNGT 5 cut(s) 117, 200, 220, 254, 284
TaiI ACGT 4 cut(s) 28, 80, 100, 495
TaqI TCGA 1 cut(s) 645
TaqII GACCGA 1 cut(s) 307
TasI AATT 3 cut(s) 346, 390, 694
TatI WGTACW 3 cut(s) 200, 220, 284
TauI GCSGC 2 cut(s) 39, 61
Tru1I TTAA 1 cut(s) 350
Tru9I TTAA 1 cut(s) 350
TscAI CASTG 2 cut(s) 90, 684
TseFI GTSAC 1 cut(s) 497
TseI GCWGC 4 cut(s) 4, 29, 44, 478
Tsp45I GTSAC 1 cut(s) 497
TspDTI ATGAA 3 cut(s) 228, 425, 444
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 2 cut(s) 90, 684
VpaK11BI GGWCC 1 cut(s) 88
XagI CCTNNNNNAGG 1 cut(s) 440
XapI RAATTY 1 cut(s) 346
XcmI CCANNNNNNNNNTGG 2 cut(s) 82, 459
ZrmI AGTACT 3 cut(s) 202, 222, 286
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.