Rmu_sc0005255.1_g000006

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005255.1
N/A
Physical Location & Seq
Reverse (-)
20621 .. 22006
1386 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1386 bp
atgaatagcagtgatatagaaaagggtaaagcggatgaaagcgtttcaattcatcaagaccggagaggaatgactattgaacaatggaaacgcaccttcaattctgagcttttgaaggccaaatcatcacctgtgcctgtttcatgttgcatcttcaaagttcctgaggtgctacgaagacaagaaaaggaggcatacgaacctgacgtagtctcaatcggtccctttcaccgaggtgttgataaatttcaactcgtggaaaatgtgaaaaaatggtatttaaacagctgtcttttcttgccccagcgccagcgccaccgccagacaatattgaacatggatagcttgattgaaagcatcgagggacaggtaacacgcgcccggggttattattcagacccatgctatcatcttgaccaaaacgacttcatagagatgctcatactcgatggttgtttcttaatagaattatttcggaaggcaccttctgagcatgcaaaagatcgacaagatatagacgaccctatcttcaacatgccttgtatgctcgaatacctgtaccatgatcttctgttgctagaaaatcagctaccctggtttgtgctcgagtctatctataacctaattgaggatggtagccctatagcacccctcagtcaagtggtaatagacttcttcaaaaattcagtggctgaacctaaaatttttagctatcccaagtctcccaaggttgagattcttcacatacttgatctgataagaacttccctcgttcaggaccatctggaacatgcagaccaaaaggagacccccgcatcaaagttggtaaaccacttgccacagaggatacctagtgctgctgctctttcagaggcgggagttaaatttagagagggcccctcaggttgcatattgaacataacgttcaaaggtggcattttcaccattcctcctctggctatcgatgaaaggacaggtcctatgttcaggaaccttatggcctacgaacagtgttattggagttgcaaacacatgataacatcctacgcggttttgatggataaccttatcgatacgaacaaggatgtggatcttctatgtgaaaaacatattctagccaactggttgagtgccgatgatgcttcgaagttcttcaacgatctatacaatgacaccacagtcattgactatgtctacaagggagtttgcgatcaagtgcattcatatcgcaaagtaccatggcacaagtggagaaagaaattgaaacgtgattattttgaaacaccatggtctactatctcttttattgcagcttttgtcctcctggtccttaccttcttgcagaccatatacacactccaaccgtactatcatccccgctcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

461

Amino Acids

53.52

Weight (kDa)

6.03

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 51 - 444 9.5e-114 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1178
AccB1I GGYRCC 1 cut(s) 481
AccBSI CCGCTC 1 cut(s) 1380
AccI GTMKAC 2 cut(s) 1194, 1292
AccII CGCG 2 cut(s) 378, 1049
AciI CCGC 6 cut(s) 32, 319, 813, 875, 1049, 1378
AclI AACGTT 1 cut(s) 923
AclWI GGATC 1 cut(s) 1098
AcsI RAATTY 4 cut(s) 245, 682, 702, 884
AfaI GTAC 3 cut(s) 560, 1236, 1367
AfiI CCNNNNNNNGG 2 cut(s) 628, 775
AjnI CCWGG 2 cut(s) 593, 1323
AleI CACNNNNGTG 1 cut(s) 234
AluBI AGCT 6 cut(s) 109, 288, 345, 589, 711, 1313
AluI AGCT 6 cut(s) 109, 288, 345, 589, 711, 1313
Alw21I GWGCWC 1 cut(s) 606
Alw26I GTCTC 3 cut(s) 217, 726, 800
AlwI GGATC 1 cut(s) 1098
AlwNI CAGNNNCTG 1 cut(s) 692
Ama87I CYCGRG 2 cut(s) 381, 605
AoxI GGCC 3 cut(s) 117, 895, 999
ApaI GGGCCC 1 cut(s) 899
ApeKI GCWGC 3 cut(s) 857, 860, 1310
ApoI RAATTY 4 cut(s) 245, 682, 702, 884
Asp700I GAANNNNTTC 2 cut(s) 471, 1151
AspLEI GCGC 3 cut(s) 309, 315, 380
AspS9I GGNCC 6 cut(s) 221, 778, 895, 896, 977, 1327
AsuC2I CCSGG 2 cut(s) 382, 383
AsuHPI GGTGA 3 cut(s) 120, 221, 934
AsuII TTCGAA 1 cut(s) 1145
AvaI CYCGRG 2 cut(s) 381, 605
AvaII GGWCC 4 cut(s) 221, 778, 977, 1327
AxyI CCTNAGG 2 cut(s) 165, 901
BaeGI GKGCMC 1 cut(s) 899
BanI GGYRCC 1 cut(s) 481
BanII GRGCYC 1 cut(s) 899
BauI CACGAG 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 184
Bbv12I GWGCWC 1 cut(s) 606
BbvI GCAGC 3 cut(s) 844, 847, 1322
BccI CCATC 4 cut(s) 443, 626, 789, 1051
BcgI CGANNNNNNTGC 2 cut(s) 1208, 1242
BciT130I CCWGG 2 cut(s) 595, 1325
BciVI GTATCC 1 cut(s) 840
BcnI CCSGG 2 cut(s) 382, 383
BcoDI GTCTC 3 cut(s) 217, 726, 800
BfaI CTAG 3 cut(s) 578, 852, 1115
BfmI CTRYAG 1 cut(s) 642
BfoI RGCGCY 2 cut(s) 310, 316
BfuI GTATCC 1 cut(s) 840
BisI GCNGC 3 cut(s) 858, 861, 1311
BlsI GCNGC 3 cut(s) 859, 862, 1312
Bme1390I CCNGG 4 cut(s) 382, 383, 595, 1325
Bme18I GGWCC 4 cut(s) 221, 778, 977, 1327
BmeT110I CYCGRG 2 cut(s) 381, 605
BmgT120I GGNCC 6 cut(s) 221, 778, 895, 896, 977, 1327
BmiI GGNNCC 5 cut(s) 223, 483, 897, 898, 992
BmrFI CCNGG 4 cut(s) 382, 383, 595, 1325
BmsI GCATC 5 cut(s) 159, 366, 426, 824, 1129
BpiI GAAGAC 1 cut(s) 184
BplI GAGNNNNNCTC 2 cut(s) 884, 916
Bpu14I TTCGAA 1 cut(s) 1145
BpuMI CCSGG 2 cut(s) 382, 383
Bsa29I ATCGAT 2 cut(s) 963, 1071
BsaBI GATNNNNATC 1 cut(s) 1089
BsaI GGTCTC 1 cut(s) 800
BsaJI CCNNGG 7 cut(s) 232, 381, 382, 593, 726, 1238, 1286
BsaWI WCCGGW 1 cut(s) 60
BsaXI ACNNNNNCTCC 2 cut(s) 934, 964
Bsc4I CCNNNNNNNGG 2 cut(s) 628, 775
Bse1I ACTGG 1 cut(s) 1127
Bse21I CCTNAGG 2 cut(s) 165, 901
Bse8I GATNNNNATC 1 cut(s) 1089
BseBI CCWGG 2 cut(s) 595, 1325
BseCI ATCGAT 2 cut(s) 963, 1071
BseDI CCNNGG 7 cut(s) 232, 381, 382, 593, 726, 1238, 1286
BseGI GGATG 5 cut(s) 40, 637, 1040, 1090, 1372
BseJI GATNNNNATC 1 cut(s) 1089
BseLI CCNNNNNNNGG 2 cut(s) 628, 775
BseMII CTCAG 5 cut(s) 96, 156, 480, 667, 915
BseNI ACTGG 1 cut(s) 1127
BseRI GAGGAG 1 cut(s) 942
BseSI GKGCMC 1 cut(s) 899
BseXI GCAGC 3 cut(s) 844, 847, 1322
BseYI CCCAGC 1 cut(s) 303
Bsh1236I CGCG 2 cut(s) 378, 1049
BshFI GGCC 3 cut(s) 119, 897, 1001
BshNI GGYRCC 1 cut(s) 481
BshVI ATCGAT 2 cut(s) 963, 1071
BsiHKAI GWGCWC 1 cut(s) 606
BsiHKCI CYCGRG 2 cut(s) 381, 605
BsiSI CCGG 2 cut(s) 61, 382
BslFI GGGAC 2 cut(s) 207, 378
BslI CCNNNNNNNGG 2 cut(s) 628, 775
BsmAI GTCTC 3 cut(s) 217, 726, 800
BsmFI GGGAC 2 cut(s) 207, 378
BsmI GAATGC 1 cut(s) 1219
BsnI GGCC 3 cut(s) 119, 897, 1001
Bso31I GGTCTC 1 cut(s) 800
BsoBI CYCGRG 2 cut(s) 381, 605
Bsp119I TTCGAA 1 cut(s) 1145
Bsp120I GGGCCC 1 cut(s) 895
Bsp1286I GDGCHC 2 cut(s) 606, 899
Bsp143I GATC 6 cut(s) 502, 565, 751, 1090, 1159, 1210
Bsp19I CCATGG 2 cut(s) 1238, 1286
BspACI CCGC 6 cut(s) 32, 319, 813, 875, 1049, 1378
BspANI GGCC 3 cut(s) 119, 897, 1001
BspCNI CTCAG 5 cut(s) 97, 157, 481, 666, 914
BspDI ATCGAT 2 cut(s) 963, 1071
BspFNI CGCG 2 cut(s) 378, 1049
BspHI TCATGA 1 cut(s) 1382
BspLI GGNNCC 5 cut(s) 223, 483, 897, 898, 992
BspPI GGATC 1 cut(s) 1098
BspT104I TTCGAA 1 cut(s) 1145
BspT107I GGYRCC 1 cut(s) 481
BspTNI GGTCTC 1 cut(s) 800
BsrBI CCGCTC 1 cut(s) 1380
BsrI ACTGG 1 cut(s) 1127
BssECI CCNNGG 7 cut(s) 232, 381, 382, 593, 726, 1238, 1286
BssMI GATC 6 cut(s) 502, 565, 751, 1090, 1159, 1210
BssSI CACGAG 1 cut(s) 254
BssT1I CCWWGG 3 cut(s) 726, 1238, 1286
Bst2BI CACGAG 1 cut(s) 254
Bst2UI CCWGG 2 cut(s) 595, 1325
Bst4CI ACNGT 3 cut(s) 1011, 1180, 1365
BstBI TTCGAA 1 cut(s) 1145
BstC8I GCNNGC 2 cut(s) 311, 495
BstDEI CTNAG 5 cut(s) 105, 165, 489, 653, 901
BstDSI CCRYGG 2 cut(s) 1238, 1286
BstENI CCTNNNNNAGG 2 cut(s) 626, 773
BstF5I GGATG 5 cut(s) 40, 637, 1040, 1090, 1372
BstFNI CGCG 2 cut(s) 378, 1049
BstH2I RGCGCY 2 cut(s) 310, 316
BstHHI GCGC 3 cut(s) 309, 315, 380
BstKTI GATC 6 cut(s) 505, 568, 754, 1093, 1162, 1213
BstMAI GTCTC 3 cut(s) 217, 726, 800
BstMBI GATC 6 cut(s) 502, 565, 751, 1090, 1159, 1210
BstMWI GCNNNNNNNGC 2 cut(s) 544, 1139
BstNI CCWGG 2 cut(s) 595, 1325
BstNSI RCATGY 3 cut(s) 497, 538, 794
BstSCI CCNGG 4 cut(s) 380, 381, 593, 1323
BstSFI CTRYAG 1 cut(s) 642
BstSLI GKGCMC 1 cut(s) 899
BstUI CGCG 2 cut(s) 378, 1049
BstV1I GCAGC 3 cut(s) 844, 847, 1322
BstV2I GAAGAC 1 cut(s) 184
BstX2I RGATCY 1 cut(s) 1090
BstYI RGATCY 1 cut(s) 1090
Bsu15I ATCGAT 2 cut(s) 963, 1071
Bsu36I CCTNAGG 2 cut(s) 165, 901
BsuI GTATCC 1 cut(s) 840
BsuRI GGCC 3 cut(s) 119, 897, 1001
BsuTUI ATCGAT 2 cut(s) 963, 1071
BtgI CCRYGG 2 cut(s) 1238, 1286
BtsCI GGATG 5 cut(s) 40, 637, 1040, 1090, 1372
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 3 cut(s) 16, 693, 1016
Cac8I GCNNGC 2 cut(s) 311, 495
CaiI CAGNNNCTG 1 cut(s) 692
CciI TCATGA 1 cut(s) 1382
CfoI GCGC 3 cut(s) 309, 315, 380
Cfr13I GGNCC 6 cut(s) 221, 778, 895, 896, 977, 1327
Cfr9I CCCGGG 1 cut(s) 381
ClaI ATCGAT 2 cut(s) 963, 1071
Csp6I GTAC 3 cut(s) 559, 1235, 1366
CspCI CAANNNNNGTGG 2 cut(s) 1165, 1200
CviQI GTAC 3 cut(s) 559, 1235, 1366
DdeI CTNAG 5 cut(s) 105, 165, 489, 653, 901
DpnI GATC 6 cut(s) 504, 567, 753, 1092, 1161, 1212
DpnII GATC 6 cut(s) 502, 565, 751, 1090, 1159, 1210
DraI TTTAAA 1 cut(s) 282
DrdI GACNNNNNNGTC 1 cut(s) 1178
DseDI GACNNNNNNGTC 1 cut(s) 1178
Eco130I CCWWGG 3 cut(s) 726, 1238, 1286
Eco24I GRGCYC 1 cut(s) 899
Eco31I GGTCTC 1 cut(s) 800
Eco47I GGWCC 4 cut(s) 221, 778, 977, 1327
Eco81I CCTNAGG 2 cut(s) 165, 901
Eco88I CYCGRG 2 cut(s) 381, 605
EcoNI CCTNNNNNAGG 2 cut(s) 626, 773
EcoO109I RGGNCCY 3 cut(s) 895, 896, 977
EcoRII CCWGG 2 cut(s) 593, 1323
EcoT14I CCWWGG 3 cut(s) 726, 1238, 1286
EcoT38I GRGCYC 1 cut(s) 899
ErhI CCWWGG 3 cut(s) 726, 1238, 1286
FaqI GGGAC 2 cut(s) 207, 378
FauI CCCGC 2 cut(s) 820, 868
FblI GTMKAC 2 cut(s) 1194, 1292
Fnu4HI GCNGC 3 cut(s) 858, 861, 1311
FokI GGATG 5 cut(s) 47, 644, 1027, 1097, 1359
FriOI GRGCYC 1 cut(s) 899
Fsp4HI GCNGC 3 cut(s) 858, 861, 1311
FspBI CTAG 3 cut(s) 578, 852, 1115
GlaI GCGC 3 cut(s) 308, 314, 379
GluI GCNGC 3 cut(s) 858, 861, 1311
GsaI CCCAGC 1 cut(s) 307
HaeII RGCGCY 2 cut(s) 310, 316
HaeIII GGCC 3 cut(s) 119, 897, 1001
HapII CCGG 2 cut(s) 61, 382
HhaI GCGC 3 cut(s) 309, 315, 380
Hin6I GCGC 3 cut(s) 307, 313, 378
HinP1I GCGC 3 cut(s) 307, 313, 378
HinfI GANTC 2 cut(s) 608, 736
HpaII CCGG 2 cut(s) 61, 382
HphI GGTGA 3 cut(s) 120, 221, 934
Hpy166II GTNNAC 3 cut(s) 829, 1195, 1293
Hpy188I TCNGA 6 cut(s) 106, 397, 477, 490, 756, 871
Hpy188III TCNNGA 7 cut(s) 56, 164, 413, 776, 785, 988, 1383
Hpy8I GTNNAC 3 cut(s) 829, 1195, 1293
HpyAV CCTTC 5 cut(s) 106, 109, 472, 495, 1345
HpyCH4III ACNGT 3 cut(s) 1011, 1180, 1365
HpyCH4IV ACGT 3 cut(s) 207, 923, 1267
HpyCH4V TGCA 8 cut(s) 150, 497, 794, 909, 1026, 1219, 1310, 1342
HpyF10VI GCNNNNNNNGC 2 cut(s) 544, 1139
HpyF3I CTNAG 5 cut(s) 105, 165, 489, 653, 901
HpySE526I ACGT 3 cut(s) 207, 923, 1267
HspAI GCGC 3 cut(s) 307, 313, 378
Kzo9I GATC 6 cut(s) 502, 565, 751, 1090, 1159, 1210
Lsp1109I GCAGC 3 cut(s) 844, 847, 1322
LweI GCATC 5 cut(s) 159, 366, 426, 824, 1129
MaeI CTAG 3 cut(s) 578, 852, 1115
MaeII ACGT 3 cut(s) 207, 923, 1267
MaeIII GTNAC 1 cut(s) 370
MalI GATC 6 cut(s) 504, 567, 753, 1092, 1161, 1212
MbiI CCGCTC 1 cut(s) 1380
MboI GATC 6 cut(s) 502, 565, 751, 1090, 1159, 1210
MboII GAAGA 8 cut(s) 145, 189, 520, 560, 667, 731, 1085, 1144
MflI RGATCY 1 cut(s) 1090
MhlI GDGCHC 2 cut(s) 606, 899
MluCI AATT 9 cut(s) 48, 100, 245, 467, 624, 682, 702, 884, 1259
MlyI GAGTC 1 cut(s) 617
MmeI TCCRAC 1 cut(s) 1384
MroXI GAANNNNTTC 2 cut(s) 471, 1151
MseI TTAA 3 cut(s) 281, 461, 882
MslI CAYNNNNRTG 2 cut(s) 234, 434
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 2 cut(s) 61, 382
MspR9I CCNGG 4 cut(s) 382, 383, 595, 1325
Mva1269I GAATGC 1 cut(s) 1219
MvaI CCWGG 2 cut(s) 595, 1325
MvnI CGCG 2 cut(s) 378, 1049
MwoI GCNNNNNNNGC 2 cut(s) 544, 1139
NciI CCSGG 2 cut(s) 382, 383
NcoI CCATGG 2 cut(s) 1238, 1286
NdeII GATC 6 cut(s) 502, 565, 751, 1090, 1159, 1210
NlaIV GGNNCC 5 cut(s) 223, 483, 897, 898, 992
NspI RCATGY 3 cut(s) 497, 538, 794
NspV TTCGAA 1 cut(s) 1145
OliI CACNNNNGTG 1 cut(s) 234
PaeI GCATGC 1 cut(s) 497
PaeR7I CTCGAG 1 cut(s) 605
PagI TCATGA 1 cut(s) 1382
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PctI GAATGC 1 cut(s) 1219
PdmI GAANNNNTTC 2 cut(s) 471, 1151
PfeI GAWTC 1 cut(s) 736
PflFI GACNNNGTC 2 cut(s) 209, 1190
PkrI GCNGC 3 cut(s) 859, 862, 1312
PleI GAGTC 1 cut(s) 616
PpsI GAGTC 1 cut(s) 616
PpuMI RGGWCCY 1 cut(s) 977
Psp1406I AACGTT 1 cut(s) 923
Psp5II RGGWCCY 1 cut(s) 977
Psp6I CCWGG 2 cut(s) 593, 1323
PspFI CCCAGC 1 cut(s) 303
PspGI CCWGG 2 cut(s) 593, 1323
PspN4I GGNNCC 5 cut(s) 223, 483, 897, 898, 992
PspOMI GGGCCC 1 cut(s) 895
PspPI GGNCC 6 cut(s) 221, 778, 895, 896, 977, 1327
PspPPI RGGWCCY 1 cut(s) 977
PspXI VCTCGAGB 1 cut(s) 605
PstNI CAGNNNCTG 1 cut(s) 692
PsuI RGATCY 1 cut(s) 1090
PsyI GACNNNGTC 2 cut(s) 209, 1190
PvuII CAGCTG 1 cut(s) 288
RsaI GTAC 3 cut(s) 560, 1236, 1367
RsaNI GTAC 3 cut(s) 559, 1235, 1366
RseI CAYNNNNRTG 2 cut(s) 234, 434
SaqAI TTAA 3 cut(s) 281, 461, 882
SatI GCNGC 3 cut(s) 858, 861, 1311
Sau3AI GATC 6 cut(s) 502, 565, 751, 1090, 1159, 1210
Sau96I GGNCC 6 cut(s) 221, 778, 895, 896, 977, 1327
SchI GAGTC 1 cut(s) 617
ScrFI CCNGG 4 cut(s) 382, 383, 595, 1325
SduI GDGCHC 2 cut(s) 606, 899
SfaNI GCATC 5 cut(s) 159, 366, 426, 824, 1129
SfcI CTRYAG 1 cut(s) 642
Sfr274I CTCGAG 1 cut(s) 605
SfuI TTCGAA 1 cut(s) 1145
SinI GGWCC 4 cut(s) 221, 778, 977, 1327
SlaI CTCGAG 1 cut(s) 605
SmaI CCCGGG 1 cut(s) 383
SmiMI CAYNNNNRTG 2 cut(s) 234, 434
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
SphI GCATGC 1 cut(s) 497
Sse9I AATT 9 cut(s) 48, 100, 245, 467, 624, 682, 702, 884, 1259
SsiI CCGC 6 cut(s) 32, 319, 813, 875, 1049, 1378
SspI AATATT 1 cut(s) 330
SspMI CTAG 3 cut(s) 578, 852, 1115
StyD4I CCNGG 4 cut(s) 380, 381, 593, 1323
StyI CCWWGG 3 cut(s) 726, 1238, 1286
TaaI ACNGT 3 cut(s) 1011, 1180, 1365
TaiI ACGT 3 cut(s) 210, 926, 1270
TaqI TCGA 8 cut(s) 360, 447, 505, 549, 606, 963, 1071, 1145
TaqII GACCGA 1 cut(s) 209
TasI AATT 9 cut(s) 48, 100, 245, 467, 624, 682, 702, 884, 1259
TfiI GAWTC 1 cut(s) 736
Tru1I TTAA 3 cut(s) 281, 461, 882
Tru9I TTAA 3 cut(s) 281, 461, 882
TscAI CASTG 3 cut(s) 16, 693, 1016
TseI GCWGC 3 cut(s) 857, 860, 1310
TspDTI ATGAA 7 cut(s) 17, 41, 51, 132, 418, 981, 1212
TspMI CCCGGG 1 cut(s) 381
TspRI CASTG 3 cut(s) 16, 693, 1016
Tth111I GACNNNGTC 2 cut(s) 209, 1190
VpaK11BI GGWCC 4 cut(s) 221, 778, 977, 1327
XagI CCTNNNNNAGG 2 cut(s) 626, 773
XapI RAATTY 4 cut(s) 245, 682, 702, 884
XceI RCATGY 3 cut(s) 497, 538, 794
XcmI CCANNNNNNNNNTGG 2 cut(s) 952, 1245
XhoI CTCGAG 1 cut(s) 605
XmaI CCCGGG 1 cut(s) 381
XmiI GTMKAC 2 cut(s) 1194, 1292
XmnI GAANNNNTTC 2 cut(s) 471, 1151
XspI CTAG 3 cut(s) 578, 852, 1115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.