Rmu_sc0005369.1_g000003

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005369.1
N/A
Physical Location & Seq
Forward (+)
10672 .. 11010
339 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 339 bp
atgcgacccaaccggccaagcccctgtgggcagatatcgatgaggaggacgtacgtacaagtggatttggacctcctccttctgccgccaaagcaggtgattggccccgacgagaaggggatgaagaaggtgattgaatacaaatacaatgaaaaaagggtcagaaggtcaagatcaccaccactcatgagtaaggcggccgtggagcggcccgcctggccaaaatttggtggtgccgtcaatgaagacgctggtgccaatctgacagcagtttccaaagaggagatcatgctcaaacgccccaaggctcttggcgccaacaaagtgttggaattttag

Protein Analysis

112

Amino Acids

12.6

Weight (kDa)

10.05

Isoelectric Point (pI)

67.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3g PF12353 32 - 100 1.7e-09 Eukaryotic translation initiation factor 3 subunit G
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 85
Acc36I ACCTGC 1 cut(s) 85
AccB1I GGYRCC 3 cut(s) 233, 254, 314
AccB7I CCANNNNNTGG 1 cut(s) 227
AccBSI CCGCTC 1 cut(s) 208
AciI CCGC 4 cut(s) 86, 197, 208, 213
AcoI YGGCCR 3 cut(s) 14, 198, 218
AcsI RAATTY 2 cut(s) 224, 332
AcyI GRCGYC 1 cut(s) 315
AfaI GTAC 2 cut(s) 53, 57
AfiI CCNNNNNNNGG 2 cut(s) 207, 227
AgsI TTSAA 1 cut(s) 137
AjnI CCWGG 1 cut(s) 215
AoxI GGCC 5 cut(s) 14, 103, 198, 209, 218
ApoI RAATTY 2 cut(s) 224, 332
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 3 cut(s) 70, 104, 210
AsuHPI GGTGA 3 cut(s) 109, 142, 168
AvaII GGWCC 1 cut(s) 70
BalI TGGCCA 1 cut(s) 220
BanI GGYRCC 3 cut(s) 233, 254, 314
BbsI GAAGAC 1 cut(s) 252
BceAI ACGGC 2 cut(s) 185, 221
BciT130I CCWGG 1 cut(s) 217
BfoI RGCGCY 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 85
BglI GCCNNNNNGGC 1 cut(s) 217
BisI GCNGC 3 cut(s) 86, 198, 209
BlsI GCNGC 3 cut(s) 87, 199, 210
Bme1390I CCNGG 1 cut(s) 217
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 3 cut(s) 70, 104, 210
BmiI GGNNCC 4 cut(s) 106, 235, 256, 316
BmrFI CCNGG 1 cut(s) 217
BpiI GAAGAC 1 cut(s) 252
Bsa29I ATCGAT 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 55
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 2 cut(s) 201, 303
Bsc4I CCNNNNNNNGG 2 cut(s) 207, 227
Bse118I RCCGGY 1 cut(s) 12
BseBI CCWGG 1 cut(s) 217
BseCI ATCGAT 1 cut(s) 38
BseDI CCNNGG 2 cut(s) 201, 303
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 2 cut(s) 207, 227
BseRI GAGGAG 3 cut(s) 58, 65, 296
BseX3I CGGCCG 1 cut(s) 198
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 5 cut(s) 16, 105, 200, 211, 220
BshNI GGYRCC 3 cut(s) 233, 254, 314
BshVI ATCGAT 1 cut(s) 38
BsiEI CGRYCG 1 cut(s) 201
BsiSI CCGG 1 cut(s) 13
BsiWI CGTACG 1 cut(s) 51
BslI CCNNNNNNNGG 2 cut(s) 207, 227
BsnI GGCC 5 cut(s) 16, 105, 200, 211, 220
Bsp143I GATC 2 cut(s) 173, 285
BspACI CCGC 4 cut(s) 86, 197, 208, 213
BspANI GGCC 5 cut(s) 16, 105, 200, 211, 220
BspDI ATCGAT 1 cut(s) 38
BspHI TCATGA 1 cut(s) 186
BspLI GGNNCC 4 cut(s) 106, 235, 256, 316
BspMI ACCTGC 1 cut(s) 85
BspT107I GGYRCC 3 cut(s) 233, 254, 314
BsrBI CCGCTC 1 cut(s) 208
BsrFI RCCGGY 1 cut(s) 12
BssAI RCCGGY 1 cut(s) 12
BssECI CCNNGG 2 cut(s) 201, 303
BssMI GATC 2 cut(s) 173, 285
BssNI GRCGYC 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 217
BstACI GRCGYC 1 cut(s) 315
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 213
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 126
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 2 cut(s) 176, 288
BstMBI GATC 2 cut(s) 173, 285
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 3 cut(s) 91, 217, 314
BstNI CCWGG 1 cut(s) 217
BstSCI CCNGG 1 cut(s) 215
BstSNI TACGTA 1 cut(s) 55
BstV2I GAAGAC 1 cut(s) 252
BstZI CGGCCG 1 cut(s) 198
Bsu15I ATCGAT 1 cut(s) 38
BsuRI GGCC 5 cut(s) 16, 105, 200, 211, 220
BsuTUI ATCGAT 1 cut(s) 38
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 1 cut(s) 126
BveI ACCTGC 1 cut(s) 85
Cac8I GCNNGC 1 cut(s) 213
CciI TCATGA 1 cut(s) 186
CfoI GCGC 1 cut(s) 317
Cfr10I RCCGGY 1 cut(s) 12
Cfr13I GGNCC 3 cut(s) 70, 104, 210
ClaI ATCGAT 1 cut(s) 38
CseI GACGC 1 cut(s) 257
Csp6I GTAC 2 cut(s) 52, 56
CviAII CATG 2 cut(s) 187, 289
CviJI RGCY 7 cut(s) 16, 21, 105, 200, 211, 220, 308
CviKI_1 RGCY 7 cut(s) 16, 21, 105, 200, 211, 220, 308
CviQI GTAC 2 cut(s) 52, 56
DinI GGCGCC 1 cut(s) 316
DpnI GATC 2 cut(s) 175, 287
DpnII GATC 2 cut(s) 173, 285
EaeI YGGCCR 3 cut(s) 14, 198, 218
EagI CGGCCG 1 cut(s) 198
EclXI CGGCCG 1 cut(s) 198
Eco105I TACGTA 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 303
Eco32I GATATC 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 70
Eco52I CGGCCG 1 cut(s) 198
EcoRII CCWGG 1 cut(s) 215
EcoRV GATATC 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 303
EgeI GGCGCC 1 cut(s) 316
EheI GGCGCC 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 2 cut(s) 190, 292
FaiI YATR 2 cut(s) 188, 290
FatI CATG 2 cut(s) 186, 288
FauI CCCGC 1 cut(s) 220
Fnu4HI GCNGC 3 cut(s) 86, 198, 209
FokI GGATG 1 cut(s) 133
Fsp4HI GCNGC 3 cut(s) 86, 198, 209
GlaI GCGC 1 cut(s) 316
GluI GCNGC 3 cut(s) 86, 198, 209
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 5 cut(s) 16, 105, 200, 211, 220
HapII CCGG 1 cut(s) 13
HgaI GACGC 1 cut(s) 257
HhaI GCGC 1 cut(s) 317
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 2 cut(s) 190, 292
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HpaII CCGG 1 cut(s) 13
HphI GGTGA 3 cut(s) 109, 142, 168
Hpy188I TCNGA 2 cut(s) 164, 264
Hpy188III TCNNGA 2 cut(s) 171, 187
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 4 cut(s) 89, 109, 121, 159
HpyCH4IV ACGT 2 cut(s) 50, 54
HpyF10VI GCNNNNNNNGC 3 cut(s) 91, 217, 314
HpySE526I ACGT 2 cut(s) 50, 54
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 2 cut(s) 190, 292
HspAI GCGC 1 cut(s) 315
KasI GGCGCC 1 cut(s) 314
Kzo9I GATC 2 cut(s) 173, 285
LmnI GCTCC 1 cut(s) 205
LpnPI CCDG 6 cut(s) 26, 37, 80, 202, 229, 237
MaeII ACGT 2 cut(s) 50, 54
MalI GATC 2 cut(s) 175, 287
MbiI CCGCTC 1 cut(s) 208
MboI GATC 2 cut(s) 173, 285
MboII GAAGA 2 cut(s) 136, 257
MlsI TGGCCA 1 cut(s) 220
MluCI AATT 2 cut(s) 224, 332
MluNI TGGCCA 1 cut(s) 220
Mly113I GGCGCC 1 cut(s) 315
MmeI TCCRAC 1 cut(s) 309
MnlI CCTC 5 cut(s) 36, 39, 83, 86, 274
Mox20I TGGCCA 1 cut(s) 220
MscI TGGCCA 1 cut(s) 220
Msp20I TGGCCA 1 cut(s) 220
MspI CCGG 1 cut(s) 13
MspR9I CCNGG 1 cut(s) 217
MvaI CCWGG 1 cut(s) 217
MwoI GCNNNNNNNGC 3 cut(s) 91, 217, 314
NarI GGCGCC 1 cut(s) 315
NdeII GATC 2 cut(s) 173, 285
NlaIII CATG 2 cut(s) 190, 292
NlaIV GGNNCC 4 cut(s) 106, 235, 256, 316
PagI TCATGA 1 cut(s) 186
PaqCI CACCTGC 1 cut(s) 85
Pfl23II CGTACG 1 cut(s) 51
PflMI CCANNNNNTGG 1 cut(s) 227
PkrI GCNGC 3 cut(s) 87, 199, 210
PluTI GGCGCC 1 cut(s) 318
Ppu21I YACGTR 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 215
PspGI CCWGG 1 cut(s) 215
PspLI CGTACG 1 cut(s) 51
PspN4I GGNNCC 4 cut(s) 106, 235, 256, 316
PspPI GGNCC 3 cut(s) 70, 104, 210
RsaI GTAC 2 cut(s) 53, 57
RsaNI GTAC 2 cut(s) 52, 56
SatI GCNGC 3 cut(s) 86, 198, 209
Sau3AI GATC 2 cut(s) 173, 285
Sau96I GGNCC 3 cut(s) 70, 104, 210
ScrFI CCNGG 1 cut(s) 217
SetI ASST 6 cut(s) 53, 57, 75, 99, 132, 170
SfiI GGCCNNNNNGGCC 1 cut(s) 217
SfoI GGCGCC 1 cut(s) 316
SinI GGWCC 1 cut(s) 70
SnaBI TACGTA 1 cut(s) 55
Sse9I AATT 2 cut(s) 224, 332
SsiI CCGC 4 cut(s) 86, 197, 208, 213
SspDI GGCGCC 1 cut(s) 314
StyD4I CCNGG 1 cut(s) 215
StyI CCWWGG 1 cut(s) 303
TaiI ACGT 2 cut(s) 53, 57
TaqI TCGA 1 cut(s) 38
TasI AATT 2 cut(s) 224, 332
TauI GCSGC 3 cut(s) 88, 200, 211
TspDTI ATGAA 3 cut(s) 137, 165, 258
Van91I CCANNNNNTGG 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 70
XapI RAATTY 2 cut(s) 224, 332
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.