Rmu_sc0005645.1_g000022

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005645.1
N/A
Physical Location & Seq
Reverse (-)
111885 .. 115367
3483 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 483 bp
ctggggggcaataatagccggattctggaatccggtcaccggtcgtcggaatccggtcaccgttcaccggagtccagtcaccgttcgccggagtccggtcaccggttgccggagtctggttgccggagactgcgccggccactagtcaccgtagcacagcaaggtggaagggatcccgtaccttcagaatcgcttaaaaggaccaaagtggcttcctttcttagttggggtagatctcatatgcttcttttcttttggcatcccagtttccataaccatgagagtgttacattacttcctcctctgctactcttttcgggtgctagtgctgcatttggaggttatgcacttccaaagtttgctcaactcacggtgacttcgtattatgccacctcaagtgcctcacattacgggatttcgcttcttactcgacatattgaagagactcatacttcccgctctcagcaagaaagactcggatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

17.69

Weight (kDa)

10.32

Isoelectric Point (pI)

90.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 459
AciI CCGC 1 cut(s) 457
AclWI GGATC 2 cut(s) 167, 180
AcoI YGGCCR 1 cut(s) 137
AcuI CTGAAG 1 cut(s) 168
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 9 cut(s) 25, 39, 46, 67, 88, 95, 102, 109, 116
AgeI ACCGGT 2 cut(s) 39, 102
AgsI TTSAA 1 cut(s) 440
AhlI ACTAGT 1 cut(s) 142
Alw26I GTCTC 2 cut(s) 121, 437
AlwI GGATC 2 cut(s) 167, 180
AoxI GGCC 1 cut(s) 137
ApeKI GCWGC 1 cut(s) 329
AsiGI ACCGGT 2 cut(s) 39, 102
AspLEI GCGC 1 cut(s) 135
AspS9I GGNCC 1 cut(s) 201
AsuHPI GGTGA 7 cut(s) 29, 50, 57, 71, 92, 139, 385
AvaII GGWCC 1 cut(s) 201
BamHI GGATCC 1 cut(s) 172
BbvI GCAGC 1 cut(s) 316
BcoDI GTCTC 2 cut(s) 121, 437
BcuI ACTAGT 1 cut(s) 142
BfaI CTAG 2 cut(s) 143, 324
BglII AGATCT 1 cut(s) 233
BisI GCNGC 1 cut(s) 330
BlsI GCNGC 1 cut(s) 331
Bme18I GGWCC 1 cut(s) 201
BmgT120I GGNCC 1 cut(s) 201
BmiI GGNNCC 1 cut(s) 174
BmrI ACTGGG 1 cut(s) 258
BmsI GCATC 1 cut(s) 268
BmuI ACTGGG 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 379
BsaWI WCCGGW 6 cut(s) 32, 39, 53, 67, 95, 102
Bsc4I CCNNNNNNNGG 9 cut(s) 25, 39, 46, 67, 88, 95, 102, 109, 116
Bse118I RCCGGY 3 cut(s) 39, 102, 135
Bse1I ACTGG 2 cut(s) 75, 264
BseGI GGATG 1 cut(s) 259
BseLI CCNNNNNNNGG 9 cut(s) 25, 39, 46, 67, 88, 95, 102, 109, 116
BseMII CTCAG 1 cut(s) 476
BseNI ACTGG 2 cut(s) 75, 264
BseRI GAGGAG 1 cut(s) 291
BseXI GCAGC 1 cut(s) 316
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 1 cut(s) 139
BshTI ACCGGT 2 cut(s) 39, 102
BsiEI CGRYCG 1 cut(s) 44
BslI CCNNNNNNNGG 9 cut(s) 25, 39, 46, 67, 88, 95, 102, 109, 116
BsmAI GTCTC 2 cut(s) 121, 437
BsnI GGCC 1 cut(s) 139
Bsp143I GATC 2 cut(s) 172, 233
BspACI CCGC 1 cut(s) 457
BspANI GGCC 1 cut(s) 139
BspCNI CTCAG 1 cut(s) 475
BspLI GGNNCC 1 cut(s) 174
BspPI GGATC 2 cut(s) 167, 180
BsrBI CCGCTC 1 cut(s) 459
BsrFI RCCGGY 3 cut(s) 39, 102, 135
BsrI ACTGG 2 cut(s) 75, 264
BssAI RCCGGY 3 cut(s) 39, 102, 135
BssMI GATC 2 cut(s) 172, 233
Bst4CI ACNGT 4 cut(s) 62, 83, 151, 373
Bst6I CTCTTC 1 cut(s) 435
BstC8I GCNNGC 1 cut(s) 137
BstDEI CTNAG 2 cut(s) 221, 462
BstEII GGTNACC 3 cut(s) 35, 56, 98
BstF5I GGATG 1 cut(s) 259
BstHHI GCGC 1 cut(s) 135
BstKTI GATC 2 cut(s) 175, 236
BstMAI GTCTC 2 cut(s) 121, 437
BstMBI GATC 2 cut(s) 172, 233
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 2 cut(s) 15, 329
BstPI GGTNACC 3 cut(s) 35, 56, 98
BstV1I GCAGC 1 cut(s) 316
BstX2I RGATCY 2 cut(s) 172, 233
BstYI RGATCY 2 cut(s) 172, 233
BsuRI GGCC 1 cut(s) 139
BtsCI GGATG 1 cut(s) 259
Cac8I GCNNGC 1 cut(s) 137
CfoI GCGC 1 cut(s) 135
Cfr10I RCCGGY 3 cut(s) 39, 102, 135
Cfr13I GGNCC 1 cut(s) 201
Csp6I GTAC 1 cut(s) 179
CspAI ACCGGT 2 cut(s) 39, 102
CviAII CATG 1 cut(s) 278
CviJI RGCY 3 cut(s) 18, 139, 212
CviKI_1 RGCY 3 cut(s) 18, 139, 212
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 2 cut(s) 221, 462
DpnI GATC 2 cut(s) 174, 235
DpnII GATC 2 cut(s) 172, 233
EaeI YGGCCR 1 cut(s) 137
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 201
Eco57I CTGAAG 1 cut(s) 168
Eco91I GGTNACC 3 cut(s) 35, 56, 98
EcoO65I GGTNACC 3 cut(s) 35, 56, 98
FaeI CATG 1 cut(s) 281
FaiI YATR 8 cut(s) 240, 242, 273, 279, 345, 387, 435, 450
FatI CATG 1 cut(s) 277
FauI CCCGC 1 cut(s) 464
FauNDI CATATG 1 cut(s) 240
Fnu4HI GCNGC 1 cut(s) 330
FokI GGATG 1 cut(s) 246
Fsp4HI GCNGC 1 cut(s) 330
FspBI CTAG 2 cut(s) 143, 324
GlaI GCGC 1 cut(s) 134
GluI GCNGC 1 cut(s) 330
HaeIII GGCC 1 cut(s) 139
HhaI GCGC 1 cut(s) 135
Hin1II CATG 1 cut(s) 281
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HinfI GANTC 9 cut(s) 22, 29, 50, 71, 92, 113, 188, 445, 474
HphI GGTGA 7 cut(s) 29, 50, 57, 71, 92, 139, 385
Hpy166II GTNNAC 1 cut(s) 65
Hpy188I TCNGA 3 cut(s) 49, 187, 479
Hpy188III TCNNGA 1 cut(s) 26
Hpy8I GTNNAC 1 cut(s) 65
Hpy99I CGWCG 1 cut(s) 49
HpyAV CCTTC 2 cut(s) 162, 192
HpyCH4III ACNGT 4 cut(s) 62, 83, 151, 373
HpyCH4V TGCA 2 cut(s) 332, 347
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 329
HpyF3I CTNAG 2 cut(s) 221, 462
Hsp92II CATG 1 cut(s) 281
HspAI GCGC 1 cut(s) 133
KroI GCCGGC 1 cut(s) 135
KroNI GCCGGC 1 cut(s) 137
Kzo9I GATC 2 cut(s) 172, 233
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 1 cut(s) 268
MaeI CTAG 2 cut(s) 143, 324
MaeIII GTNAC 7 cut(s) 35, 56, 77, 98, 145, 286, 373
MalI GATC 2 cut(s) 174, 235
MbiI CCGCTC 1 cut(s) 459
MboI GATC 2 cut(s) 172, 233
MboII GAAGA 1 cut(s) 452
MflI RGATCY 2 cut(s) 172, 233
MlyI GAGTC 5 cut(s) 80, 101, 122, 439, 468
MmeI TCCRAC 1 cut(s) 27
MnlI CCTC 5 cut(s) 309, 312, 332, 403, 412
MroNI GCCGGC 1 cut(s) 135
MseI TTAA 1 cut(s) 195
MslI CAYNNNNRTG 2 cut(s) 276, 282
MwoI GCNNNNNNNGC 2 cut(s) 15, 329
NaeI GCCGGC 1 cut(s) 137
NdeI CATATG 1 cut(s) 240
NdeII GATC 2 cut(s) 172, 233
NgoMIV GCCGGC 1 cut(s) 135
NlaIII CATG 1 cut(s) 281
NlaIV GGNNCC 1 cut(s) 174
NmuCI GTSAC 6 cut(s) 35, 56, 77, 98, 145, 373
PcsI WCGNNNNNNNCGW 1 cut(s) 377
PdiI GCCGGC 1 cut(s) 137
PfeI GAWTC 4 cut(s) 22, 29, 50, 188
PinAI ACCGGT 2 cut(s) 39, 102
PkrI GCNGC 1 cut(s) 331
PleI GAGTC 5 cut(s) 79, 100, 121, 439, 468
PpsI GAGTC 5 cut(s) 79, 100, 121, 439, 468
PspEI GGTNACC 3 cut(s) 35, 56, 98
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 1 cut(s) 201
PsuI RGATCY 2 cut(s) 172, 233
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
RseI CAYNNNNRTG 2 cut(s) 276, 282
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 1 cut(s) 330
Sau3AI GATC 2 cut(s) 172, 233
Sau96I GGNCC 1 cut(s) 201
SchI GAGTC 5 cut(s) 80, 101, 122, 439, 468
SetI ASST 4 cut(s) 166, 184, 343, 395
SfaNI GCATC 1 cut(s) 268
SinI GGWCC 1 cut(s) 201
SmiMI CAYNNNNRTG 2 cut(s) 276, 282
SmlI CTYRAG 1 cut(s) 394
SmoI CTYRAG 1 cut(s) 394
SpeI ACTAGT 1 cut(s) 142
SsiI CCGC 1 cut(s) 457
SspMI CTAG 2 cut(s) 143, 324
TaaI ACNGT 4 cut(s) 62, 83, 151, 373
TaqI TCGA 1 cut(s) 430
TfiI GAWTC 4 cut(s) 22, 29, 50, 188
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TseFI GTSAC 6 cut(s) 35, 56, 77, 98, 145, 373
TseI GCWGC 1 cut(s) 329
Tsp45I GTSAC 6 cut(s) 35, 56, 77, 98, 145, 373
VpaK11BI GGWCC 1 cut(s) 201
XspI CTAG 2 cut(s) 143, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.