Rmu_sc0006016.1_g000014

Hyccin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006016.1
N/A
Physical Location & Seq
Reverse (-)
45709 .. 48517
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
atggatgaagataaactcagttacaatcaaaaaacttttagagaggatccttcttctgtctcaaggttgtcacctttgagagtcggtggcgtgctccgatcacaggtggctccatccgcccccctgacggcaccgtctgtcctcttgacggctatgtccatcttcttgatagctccatccagttctcctgccggcggcggagtctatccttttccattgttcttttctgtcttctcttctcctcgccttaatttgttcttattttattcttttgtaaagcctctaaccctgctttttaccatgttgctcgtgcctaagaacactaggtacggttcctatgcatttggcaaaatggagatacacatagcagcctcttgggtgcaatcactgacaccttcattgggtttcttgttggtttctaacggctccatcgtcttttgcttcacggctgcgattttccttgttgatctgtgtttgatgctagagtgttggttcatagcttcttgggccggccaggattgctattgtgaacggcaattcaaatccggtgataaccatagtgagtctgagattgctgggttcttggaggccagcaattggaatgggagtggtaatgagagtgaaattgagattgagggtgatgtggtagaagatatgggtcaactgagaattgtgcaaaatggtagcagcggtggtgtgagtcatgtggaggagtcaaagggggcgaggattccgttaccgtgggagcttttgcagccattcacacttgaaagttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hyccin

Protein Analysis

258

Amino Acids

28.26

Weight (kDa)

4.83

Isoelectric Point (pI)

59.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 133, 154
AccB1I GGYRCC 1 cut(s) 130
AccB7I CCANNNNNTGG 1 cut(s) 597
AciI CCGC 4 cut(s) 117, 195, 198, 690
AclWI GGATC 2 cut(s) 41, 54
AcoI YGGCCR 1 cut(s) 511
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 6 cut(s) 103, 127, 148, 194, 401, 597
AgsI TTSAA 2 cut(s) 541, 770
AjnI CCWGG 1 cut(s) 513
AluBI AGCT 3 cut(s) 173, 500, 748
AluI AGCT 3 cut(s) 173, 500, 748
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 64
AlwI GGATC 2 cut(s) 41, 54
AoxI GGCC 3 cut(s) 507, 511, 588
ApeKI GCWGC 4 cut(s) 368, 449, 687, 754
AspS9I GGNCC 1 cut(s) 507
AsuHPI GGTGA 3 cut(s) 63, 560, 650
BamHI GGATCC 1 cut(s) 46
BanI GGYRCC 1 cut(s) 130
BauI CACGAG 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 4 cut(s) 380, 436, 699, 766
BccI CCATC 4 cut(s) 121, 167, 184, 437
BceAI ACGGC 5 cut(s) 144, 165, 439, 462, 548
BciT130I CCWGG 1 cut(s) 515
BcoDI GTCTC 1 cut(s) 64
BfaI CTAG 2 cut(s) 324, 482
BisI GCNGC 5 cut(s) 196, 369, 450, 688, 755
BlsI GCNGC 5 cut(s) 197, 370, 451, 689, 756
Bme1390I CCNGG 1 cut(s) 515
BmgT120I GGNCC 1 cut(s) 507
BmiI GGNNCC 5 cut(s) 48, 111, 132, 334, 427
BmrFI CCNGG 1 cut(s) 515
BmsI GCATC 1 cut(s) 468
BpiI GAAGAC 1 cut(s) 223
BpuEI CTTGAG 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 740
BsaWI WCCGGW 1 cut(s) 545
Bsc4I CCNNNNNNNGG 6 cut(s) 103, 127, 148, 194, 401, 597
Bse118I RCCGGY 2 cut(s) 191, 509
Bse1I ACTGG 1 cut(s) 180
BseBI CCWGG 1 cut(s) 515
BseDI CCNNGG 1 cut(s) 740
BseGI GGATG 3 cut(s) 10, 113, 176
BseLI CCNNNNNNNGG 6 cut(s) 103, 127, 148, 194, 401, 597
BseMII CTCAG 3 cut(s) 31, 558, 656
BseNI ACTGG 1 cut(s) 180
BseRI GAGGAG 2 cut(s) 231, 725
BseXI GCAGC 4 cut(s) 380, 436, 699, 766
BseYI CCCAGC 1 cut(s) 575
BshFI GGCC 3 cut(s) 509, 513, 590
BshNI GGYRCC 1 cut(s) 130
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 3 cut(s) 192, 510, 546
BslI CCNNNNNNNGG 6 cut(s) 103, 127, 148, 194, 401, 597
BsmAI GTCTC 1 cut(s) 64
BsnI GGCC 3 cut(s) 509, 513, 590
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 3 cut(s) 46, 98, 466
BspACI CCGC 4 cut(s) 117, 195, 198, 690
BspANI GGCC 3 cut(s) 509, 513, 590
BspCNI CTCAG 3 cut(s) 30, 559, 657
BspLI GGNNCC 5 cut(s) 48, 111, 132, 334, 427
BspPI GGATC 2 cut(s) 41, 54
BspT107I GGYRCC 1 cut(s) 130
BsrFI RCCGGY 2 cut(s) 191, 509
BsrI ACTGG 1 cut(s) 180
BssAI RCCGGY 2 cut(s) 191, 509
BssECI CCNNGG 1 cut(s) 740
BssMI GATC 3 cut(s) 46, 98, 466
BssSI CACGAG 1 cut(s) 308
Bst2BI CACGAG 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 515
Bst4CI ACNGT 3 cut(s) 135, 332, 741
Bst6I CTCTTC 1 cut(s) 241
BstC8I GCNNGC 4 cut(s) 92, 193, 511, 592
BstDEI CTNAG 4 cut(s) 17, 315, 567, 665
BstDSI CCRYGG 1 cut(s) 740
BstF5I GGATG 3 cut(s) 10, 113, 176
BstKTI GATC 3 cut(s) 49, 101, 469
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 3 cut(s) 46, 98, 466
BstMWI GCNNNNNNNGC 4 cut(s) 116, 506, 519, 754
BstNI CCWGG 1 cut(s) 515
BstSCI CCNGG 1 cut(s) 513
BstV1I GCAGC 4 cut(s) 380, 436, 699, 766
BstV2I GAAGAC 1 cut(s) 223
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BsuRI GGCC 3 cut(s) 509, 513, 590
BtgI CCRYGG 1 cut(s) 740
BtsCI GGATG 3 cut(s) 10, 113, 176
BtsIMutI CAGTG 1 cut(s) 386
Cac8I GCNNGC 4 cut(s) 92, 193, 511, 592
Cfr10I RCCGGY 2 cut(s) 191, 509
Cfr13I GGNCC 1 cut(s) 507
Csp6I GTAC 1 cut(s) 328
CviAII CATG 2 cut(s) 301, 704
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 4 cut(s) 17, 315, 567, 665
DpnI GATC 3 cut(s) 48, 100, 468
DpnII GATC 3 cut(s) 46, 98, 466
DrdI GACNNNNNNGTC 2 cut(s) 133, 154
DseDI GACNNNNNNGTC 2 cut(s) 133, 154
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 1 cut(s) 241
EarI CTCTTC 1 cut(s) 241
EciI GGCGGA 2 cut(s) 106, 213
EcoRII CCWGG 1 cut(s) 513
EcoT22I ATGCAT 1 cut(s) 343
FaeI CATG 2 cut(s) 304, 707
FaiI YATR 8 cut(s) 155, 302, 339, 365, 497, 558, 656, 705
FatI CATG 2 cut(s) 300, 703
Fnu4HI GCNGC 5 cut(s) 196, 369, 450, 688, 755
FokI GGATG 3 cut(s) 17, 100, 163
FseI GGCCGGCC 1 cut(s) 513
Fsp4HI GCNGC 5 cut(s) 196, 369, 450, 688, 755
FspBI CTAG 2 cut(s) 324, 482
GluI GCNGC 5 cut(s) 196, 369, 450, 688, 755
GsaI CCCAGC 1 cut(s) 579
HaeIII GGCC 3 cut(s) 509, 513, 590
HapII CCGG 3 cut(s) 192, 510, 546
Hin1II CATG 2 cut(s) 304, 707
HincII GTYRAC 1 cut(s) 662
HindII GTYRAC 1 cut(s) 662
HinfI GANTC 6 cut(s) 81, 201, 563, 700, 713, 730
HpaII CCGG 3 cut(s) 192, 510, 546
HphI GGTGA 3 cut(s) 63, 560, 650
Hpy166II GTNNAC 2 cut(s) 530, 662
Hpy188I TCNGA 2 cut(s) 98, 568
Hpy188III TCNNGA 2 cut(s) 145, 166
Hpy8I GTNNAC 2 cut(s) 530, 662
HpyAV CCTTC 2 cut(s) 60, 405
HpyCH4III ACNGT 3 cut(s) 135, 332, 741
HpyCH4V TGCA 4 cut(s) 341, 382, 676, 754
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 506, 519, 754
HpyF3I CTNAG 4 cut(s) 17, 315, 567, 665
Hsp92II CATG 2 cut(s) 304, 707
KroI GCCGGC 2 cut(s) 191, 509
KroNI GCCGGC 2 cut(s) 193, 511
Kzo9I GATC 3 cut(s) 46, 98, 466
LmnI GCTCC 5 cut(s) 99, 115, 178, 431, 745
Lsp1109I GCAGC 4 cut(s) 380, 436, 699, 766
LweI GCATC 1 cut(s) 468
MaeI CTAG 2 cut(s) 324, 482
MaeIII GTNAC 3 cut(s) 20, 69, 735
MalI GATC 3 cut(s) 48, 100, 468
MboI GATC 3 cut(s) 46, 98, 466
MboII GAAGA 6 cut(s) 20, 45, 154, 223, 228, 662
MfeI CAATTG 1 cut(s) 595
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 5 cut(s) 250, 536, 595, 624, 669
MlyI GAGTC 5 cut(s) 90, 210, 572, 709, 722
MnlI CCTC 9 cut(s) 37, 152, 252, 291, 382, 580, 628, 703, 720
Mph1103I ATGCAT 1 cut(s) 343
MroNI GCCGGC 2 cut(s) 191, 509
MseI TTAA 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 690
MspI CCGG 3 cut(s) 192, 510, 546
MspR9I CCNGG 1 cut(s) 515
MunI CAATTG 1 cut(s) 595
MvaI CCWGG 1 cut(s) 515
MwoI GCNNNNNNNGC 4 cut(s) 116, 506, 519, 754
NaeI GCCGGC 2 cut(s) 193, 511
NdeII GATC 3 cut(s) 46, 98, 466
NgoMIV GCCGGC 2 cut(s) 191, 509
NlaIII CATG 2 cut(s) 304, 707
NlaIV GGNNCC 5 cut(s) 48, 111, 132, 334, 427
NmuCI GTSAC 1 cut(s) 69
NsiI ATGCAT 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PdiI GCCGGC 2 cut(s) 193, 511
PfeI GAWTC 1 cut(s) 730
PflMI CCANNNNNTGG 1 cut(s) 597
PkrI GCNGC 5 cut(s) 197, 370, 451, 689, 756
PleI GAGTC 5 cut(s) 89, 209, 571, 708, 721
PpsI GAGTC 5 cut(s) 89, 209, 571, 708, 721
Psp6I CCWGG 1 cut(s) 513
PspFI CCCAGC 1 cut(s) 575
PspGI CCWGG 1 cut(s) 513
PspN4I GGNNCC 5 cut(s) 48, 111, 132, 334, 427
PspPI GGNCC 1 cut(s) 507
PsrI GAACNNNNNNTAC 2 cut(s) 311, 343
PsuI RGATCY 1 cut(s) 46
RigI GGCCGGCC 1 cut(s) 513
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 5 cut(s) 196, 369, 450, 688, 755
Sau3AI GATC 3 cut(s) 46, 98, 466
Sau96I GGNCC 1 cut(s) 507
SchI GAGTC 5 cut(s) 90, 210, 572, 709, 722
ScrFI CCNGG 1 cut(s) 515
SduI GDGCHC 1 cut(s) 96
SetI ASST 8 cut(s) 68, 76, 108, 175, 329, 397, 502, 750
SfaNI GCATC 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
Sse9I AATT 5 cut(s) 250, 536, 595, 624, 669
SsiI CCGC 4 cut(s) 117, 195, 198, 690
SspMI CTAG 2 cut(s) 324, 482
StyD4I CCNGG 1 cut(s) 513
TaaI ACNGT 3 cut(s) 135, 332, 741
TasI AATT 5 cut(s) 250, 536, 595, 624, 669
TauI GCSGC 1 cut(s) 198
TfiI GAWTC 1 cut(s) 730
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 69
TseI GCWGC 4 cut(s) 368, 449, 687, 754
Tsp45I GTSAC 1 cut(s) 69
TspDTI ATGAA 3 cut(s) 21, 387, 484
TspGWI ACGGA 1 cut(s) 723
TspRI CASTG 1 cut(s) 393
Van91I CCANNNNNTGG 1 cut(s) 597
XspI CTAG 2 cut(s) 324, 482
Zsp2I ATGCAT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.