Rmu_sc0006032.1_g000006

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006032.1
N/A
Physical Location & Seq
Forward (+)
29700 .. 30854
1155 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 498 bp
atgttggcgatacagctatttttcggtggagaggagcggacggaatcggagacgagattgaagagggtgccgctcgtcaacatagagcggacgggctggatgtggaaggcgtttttgccggaggggtcggatcgggaccacccggtggtcaatgtgttcgggccgaacgcggtggatatttcgggggttgattttccggcgacgattatgttggttgggggatttgatccagaattgacgaccgcgagcttcgccgctcactccgagttcatccccgctaccaccgacgacgacttagccatcaccactctcattctctccttcgacccccttttatacaccattgatcagaaactcagacctcgcgagccctctactctcaaatcacagctccaatcccaccctgccatcctctccgtcattcccgacatgcaacaccatctccacaccatcctcacctgcaacttcatcggtctatccgacaacttcggcctctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

165

Amino Acids

18.32

Weight (kDa)

4.83

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 11 - 81 1.9e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 467
Acc36I ACCTGC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 67
AccB7I CCANNNNNTGG 1 cut(s) 145
AccBSI CCGCTC 4 cut(s) 37, 73, 88, 257
AccII CGCG 3 cut(s) 170, 245, 366
AciI CCGC 7 cut(s) 37, 71, 88, 170, 243, 255, 276
AclWI GGATC 2 cut(s) 138, 221
AdeI CACNNNGTG 1 cut(s) 145
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 1 cut(s) 61
AluBI AGCT 3 cut(s) 16, 249, 391
AluI AGCT 3 cut(s) 16, 249, 391
Alw26I GTCTC 1 cut(s) 44
AlwI GGATC 2 cut(s) 138, 221
AoxI GGCC 2 cut(s) 161, 490
AspS9I GGNCC 2 cut(s) 136, 161
AsuC2I CCSGG 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 295, 448
AvaII GGWCC 1 cut(s) 136
BanI GGYRCC 1 cut(s) 67
BanII GRGCYC 1 cut(s) 372
BccI CCATC 4 cut(s) 308, 416, 447, 458
BcgI CGANNNNNNTGC 2 cut(s) 451, 485
BclI TGATCA 1 cut(s) 346
BcnI CCSGG 1 cut(s) 143
BcoDI GTCTC 1 cut(s) 44
BfaI CTAG 1 cut(s) 496
BfuAI ACCTGC 1 cut(s) 467
BisI GCNGC 2 cut(s) 71, 255
BlsI GCNGC 2 cut(s) 72, 256
Bme1390I CCNGG 1 cut(s) 143
Bme18I GGWCC 1 cut(s) 136
BmgT120I GGNCC 2 cut(s) 136, 161
BmiI GGNNCC 2 cut(s) 69, 137
BmrFI CCNGG 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 143
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bsc4I CCNNNNNNNGG 1 cut(s) 145
BseGI GGATG 4 cut(s) 105, 270, 408, 450
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMII CTCAG 1 cut(s) 370
BseRI GAGGAG 1 cut(s) 47
Bsh1236I CGCG 3 cut(s) 170, 245, 366
Bsh1285I CGRYCG 1 cut(s) 243
BshFI GGCC 2 cut(s) 163, 492
BshNI GGYRCC 1 cut(s) 67
BsiEI CGRYCG 1 cut(s) 243
BsiSI CCGG 3 cut(s) 119, 143, 197
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 1 cut(s) 44
BsmBI CGTCTC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 2 cut(s) 163, 492
Bsp1286I GDGCHC 1 cut(s) 372
Bsp143I GATC 3 cut(s) 130, 226, 346
Bsp68I TCGCGA 1 cut(s) 366
BspACI CCGC 7 cut(s) 37, 71, 88, 170, 243, 255, 276
BspANI GGCC 2 cut(s) 163, 492
BspCNI CTCAG 1 cut(s) 369
BspFNI CGCG 3 cut(s) 170, 245, 366
BspLI GGNNCC 2 cut(s) 69, 137
BspMI ACCTGC 1 cut(s) 467
BspPI GGATC 2 cut(s) 138, 221
BspT107I GGYRCC 1 cut(s) 67
BsrBI CCGCTC 4 cut(s) 37, 73, 88, 257
BssMI GATC 3 cut(s) 130, 226, 346
Bst6I CTCTTC 1 cut(s) 56
BstC8I GCNNGC 2 cut(s) 247, 368
BstDEI CTNAG 2 cut(s) 295, 356
BstF5I GGATG 4 cut(s) 105, 270, 408, 450
BstFNI CGCG 3 cut(s) 170, 245, 366
BstKTI GATC 3 cut(s) 133, 229, 349
BstMAI GTCTC 1 cut(s) 44
BstMBI GATC 3 cut(s) 130, 226, 346
BstMCI CGRYCG 1 cut(s) 243
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNSI RCATGY 1 cut(s) 433
BstSCI CCNGG 1 cut(s) 141
BstUI CGCG 3 cut(s) 170, 245, 366
BsuRI GGCC 2 cut(s) 163, 492
BtsCI GGATG 4 cut(s) 105, 270, 408, 450
BtuMI TCGCGA 1 cut(s) 366
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 2 cut(s) 247, 368
Cfr13I GGNCC 2 cut(s) 136, 161
CviAII CATG 1 cut(s) 430
CviJI RGCY 8 cut(s) 16, 96, 163, 249, 299, 370, 391, 492
CviKI_1 RGCY 8 cut(s) 16, 96, 163, 249, 299, 370, 391, 492
DdeI CTNAG 2 cut(s) 295, 356
DpnI GATC 3 cut(s) 132, 228, 348
DpnII GATC 3 cut(s) 130, 226, 346
DraIII CACNNNGTG 1 cut(s) 145
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco24I GRGCYC 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 136
EcoT38I GRGCYC 1 cut(s) 372
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 1 cut(s) 433
FaiI YATR 4 cut(s) 83, 209, 337, 431
FaqI GGGAC 1 cut(s) 149
FatI CATG 1 cut(s) 429
FauI CCCGC 1 cut(s) 283
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 2 cut(s) 71, 255
FokI GGATG 4 cut(s) 112, 257, 395, 437
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 2 cut(s) 71, 255
FspBI CTAG 1 cut(s) 496
GluI GCNGC 2 cut(s) 71, 255
HaeIII GGCC 2 cut(s) 163, 492
HapII CCGG 3 cut(s) 119, 143, 197
Hin1II CATG 1 cut(s) 433
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 1 cut(s) 44
HpaII CCGG 3 cut(s) 119, 143, 197
HphI GGTGA 2 cut(s) 295, 448
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 6 cut(s) 49, 130, 265, 351, 359, 481
Hpy188III TCNNGA 4 cut(s) 134, 230, 365, 425
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 3 cut(s) 205, 290, 293
HpyAV CCTTC 2 cut(s) 100, 331
HpyCH4V TGCA 2 cut(s) 433, 462
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 2 cut(s) 295, 356
Hsp92II CATG 1 cut(s) 433
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 3 cut(s) 130, 226, 346
LmnI GCTCC 2 cut(s) 34, 396
LpnPI CCDG 7 cut(s) 82, 132, 156, 210, 243, 417, 472
MaeI CTAG 1 cut(s) 496
MalI GATC 3 cut(s) 132, 228, 348
MbiI CCGCTC 4 cut(s) 37, 73, 88, 257
MboI GATC 3 cut(s) 130, 226, 346
MboII GAAGA 1 cut(s) 73
MhlI GDGCHC 1 cut(s) 372
MluCI AATT 1 cut(s) 233
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 7 cut(s) 25, 57, 115, 372, 382, 422, 464
MspI CCGG 3 cut(s) 119, 143, 197
MspR9I CCNGG 1 cut(s) 143
MvnI CGCG 3 cut(s) 170, 245, 366
MwoI GCNNNNNNNGC 1 cut(s) 251
NciI CCSGG 1 cut(s) 143
NdeII GATC 3 cut(s) 130, 226, 346
NlaIII CATG 1 cut(s) 433
NlaIV GGNNCC 2 cut(s) 69, 137
NruI TCGCGA 1 cut(s) 366
NspI RCATGY 1 cut(s) 433
PaqCI CACCTGC 1 cut(s) 467
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PfeI GAWTC 1 cut(s) 44
PflMI CCANNNNNTGG 1 cut(s) 145
PkrI GCNGC 2 cut(s) 72, 256
PspN4I GGNNCC 2 cut(s) 69, 137
PspPI GGNCC 2 cut(s) 136, 161
RruI TCGCGA 1 cut(s) 366
SatI GCNGC 2 cut(s) 71, 255
Sau3AI GATC 3 cut(s) 130, 226, 346
Sau96I GGNCC 2 cut(s) 136, 161
ScrFI CCNGG 1 cut(s) 143
SduI GDGCHC 1 cut(s) 372
SetI ASST 5 cut(s) 18, 251, 364, 393, 461
SinI GGWCC 1 cut(s) 136
Sse9I AATT 1 cut(s) 233
SsiI CCGC 7 cut(s) 37, 71, 88, 170, 243, 255, 276
SspMI CTAG 1 cut(s) 496
StyD4I CCNGG 1 cut(s) 141
TaqI TCGA 1 cut(s) 324
TaqII GACCGA 1 cut(s) 461
TasI AATT 1 cut(s) 233
TauI GCSGC 2 cut(s) 73, 257
TfiI GAWTC 1 cut(s) 44
TspDTI ATGAA 2 cut(s) 259, 457
TspGWI ACGGA 2 cut(s) 56, 406
Van91I CCANNNNNTGG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 136
XceI RCATGY 1 cut(s) 433
XspI CTAG 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.