Rmu_sc0006050.1_g000027

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006050.1
N/A
Physical Location & Seq
Forward (+)
71209 .. 76084
4876 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 600 bp
atgtctgacctgcaactcgaagatccacggtgccaacaagctcacgtcgctccaccagtgcgtgtagatgttcaaggtcagcaagcaagctccgattctctactcgcctctgtggcatttggcggtgacaccggcgataccacgtcgggattggtgcgtggtggagcagccagagttgctcagttcatgagttctgctgctgtgaaaggtcggatcaagggttttgatgctgagatgggtctgggtctacctttgggggatctaggctttgggcctcaatttttgggcttgttgttagagttttgtaagggtgctcttgaagtcggctttgtcgacacaaagaacaacctctttacgcagactcttcagacaggtgacctgtttgtgtttcccaagggacttgtgcactaccaatacaatgctgattcacaaaaccccgctctagcaatctctgcctttggaagcgcaagtgccggaactgtgtcaatccctaccactttgttcgccaccaacatcgatgacaacgtcttggctttgtccttcaagactgatgtagccaccattcaaaagctcaaggctggtcttgctcccaagccatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

199

Amino Acids

20.71

Weight (kDa)

5.03

Isoelectric Point (pI)

24.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 103 - 188 1.2e-18 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 18
AccB1I GGYRCC 1 cut(s) 30
AccBSI CCGCTC 1 cut(s) 440
AccI GTMKAC 2 cut(s) 247, 333
AciI CCGC 2 cut(s) 123, 438
AclWI GGATC 3 cut(s) 17, 221, 267
AcuI CTGAAG 1 cut(s) 350
AgsI TTSAA 4 cut(s) 74, 320, 544, 566
AjiI CACGTC 2 cut(s) 46, 144
AluBI AGCT 3 cut(s) 41, 90, 571
AluI AGCT 3 cut(s) 41, 90, 571
Alw21I GWGCWC 2 cut(s) 316, 408
Alw44I GTGCAC 1 cut(s) 404
AlwI GGATC 3 cut(s) 17, 221, 267
AoxI GGCC 1 cut(s) 272
ApaLI GTGCAC 1 cut(s) 404
ApeKI GCWGC 2 cut(s) 167, 197
AspLEI GCGC 1 cut(s) 467
AspS9I GGNCC 1 cut(s) 272
AsuHPI GGTGA 2 cut(s) 137, 386
BaeGI GKGCMC 1 cut(s) 408
BanI GGYRCC 1 cut(s) 30
Bbv12I GWGCWC 2 cut(s) 316, 408
BbvI GCAGC 2 cut(s) 179, 184
BccI CCATC 1 cut(s) 229
BfaI CTAG 2 cut(s) 263, 443
BfuAI ACCTGC 1 cut(s) 18
BglI GCCNNNNNGGC 1 cut(s) 113
BisI GCNGC 2 cut(s) 168, 198
BlsI GCNGC 2 cut(s) 169, 199
BmgBI CACGTC 2 cut(s) 46, 144
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 1 cut(s) 32
BmsI GCATC 1 cut(s) 217
BpuEI CTTGAG 1 cut(s) 557
Bsa29I ATCGAT 1 cut(s) 516
BsaJI CCNNGG 2 cut(s) 26, 393
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 1 cut(s) 56
BseCI ATCGAT 1 cut(s) 516
BseDI CCNNGG 2 cut(s) 26, 393
BseMII CTCAG 2 cut(s) 194, 222
BseNI ACTGG 1 cut(s) 56
BseSI GKGCMC 1 cut(s) 408
BseXI GCAGC 2 cut(s) 179, 184
BshFI GGCC 1 cut(s) 274
BshNI GGYRCC 1 cut(s) 30
BshVI ATCGAT 1 cut(s) 516
BsiHKAI GWGCWC 2 cut(s) 316, 408
BsiSI CCGG 2 cut(s) 132, 474
BslFI GGGAC 1 cut(s) 411
BsmFI GGGAC 1 cut(s) 411
BsnI GGCC 1 cut(s) 274
Bsp1286I GDGCHC 2 cut(s) 316, 408
Bsp143I GATC 3 cut(s) 22, 213, 259
BspACI CCGC 2 cut(s) 123, 438
BspANI GGCC 1 cut(s) 274
BspCNI CTCAG 2 cut(s) 193, 223
BspDI ATCGAT 1 cut(s) 516
BspHI TCATGA 1 cut(s) 186
BspLI GGNNCC 1 cut(s) 32
BspMI ACCTGC 1 cut(s) 18
BspPI GGATC 3 cut(s) 17, 221, 267
BspT107I GGYRCC 1 cut(s) 30
BsrBI CCGCTC 1 cut(s) 440
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 1 cut(s) 56
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 2 cut(s) 26, 393
BssMI GATC 3 cut(s) 22, 213, 259
BssT1I CCWWGG 1 cut(s) 393
Bst4CI ACNGT 2 cut(s) 30, 481
Bst6I CTCTTC 1 cut(s) 369
BstAPI GCANNNNNTGC 1 cut(s) 452
BstC8I GCNNGC 2 cut(s) 84, 88
BstDEI CTNAG 2 cut(s) 180, 231
BstDSI CCRYGG 1 cut(s) 26
BstEII GGTNACC 1 cut(s) 374
BstHHI GCGC 1 cut(s) 467
BstKTI GATC 3 cut(s) 25, 216, 262
BstMBI GATC 3 cut(s) 22, 213, 259
BstMWI GCNNNNNNNGC 5 cut(s) 47, 113, 176, 452, 584
BstPI GGTNACC 1 cut(s) 374
BstSLI GKGCMC 1 cut(s) 408
BstV1I GCAGC 2 cut(s) 179, 184
BstX2I RGATCY 2 cut(s) 22, 259
BstYI RGATCY 2 cut(s) 22, 259
Bsu15I ATCGAT 1 cut(s) 516
BsuRI GGCC 1 cut(s) 274
BsuTUI ATCGAT 1 cut(s) 516
BtgI CCRYGG 1 cut(s) 26
BtrI CACGTC 2 cut(s) 46, 144
BtsIMutI CAGTG 1 cut(s) 63
BveI ACCTGC 1 cut(s) 18
Cac8I GCNNGC 2 cut(s) 84, 88
CciI TCATGA 1 cut(s) 186
CfoI GCGC 1 cut(s) 467
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 272
ClaI ATCGAT 1 cut(s) 516
CviAII CATG 2 cut(s) 187, 597
DdeI CTNAG 2 cut(s) 180, 231
DpnI GATC 3 cut(s) 24, 215, 261
DpnII GATC 3 cut(s) 22, 213, 259
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
Eco130I CCWWGG 1 cut(s) 393
Eco57I CTGAAG 1 cut(s) 350
Eco91I GGTNACC 1 cut(s) 374
EcoO65I GGTNACC 1 cut(s) 374
EcoT14I CCWWGG 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 393
FaeI CATG 2 cut(s) 190, 600
FaiI YATR 2 cut(s) 188, 598
FaqI GGGAC 1 cut(s) 411
FatI CATG 2 cut(s) 186, 596
FauI CCCGC 1 cut(s) 445
FblI GTMKAC 2 cut(s) 247, 333
Fnu4HI GCNGC 2 cut(s) 168, 198
Fsp4HI GCNGC 2 cut(s) 168, 198
FspBI CTAG 2 cut(s) 263, 443
GlaI GCGC 1 cut(s) 466
GluI GCNGC 2 cut(s) 168, 198
HaeIII GGCC 1 cut(s) 274
HapII CCGG 2 cut(s) 132, 474
HhaI GCGC 1 cut(s) 467
Hin1II CATG 2 cut(s) 190, 600
Hin6I GCGC 1 cut(s) 465
HinP1I GCGC 1 cut(s) 465
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 3 cut(s) 95, 361, 425
HpaII CCGG 2 cut(s) 132, 474
HphI GGTGA 2 cut(s) 137, 386
Hpy166II GTNNAC 3 cut(s) 248, 334, 406
Hpy188I TCNGA 4 cut(s) 7, 94, 213, 369
Hpy188III TCNNGA 4 cut(s) 147, 187, 317, 544
Hpy8I GTNNAC 3 cut(s) 248, 334, 406
Hpy99I CGWCG 2 cut(s) 50, 148
HpyAV CCTTC 1 cut(s) 550
HpyCH4III ACNGT 2 cut(s) 30, 481
HpyCH4IV ACGT 3 cut(s) 45, 143, 525
HpyCH4V TGCA 2 cut(s) 13, 406
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 113, 176, 452, 584
HpyF3I CTNAG 2 cut(s) 180, 231
HpySE526I ACGT 3 cut(s) 45, 143, 525
Hsp92II CATG 2 cut(s) 190, 600
HspAI GCGC 1 cut(s) 465
Kzo9I GATC 3 cut(s) 22, 213, 259
LmnI GCTCC 4 cut(s) 55, 95, 164, 592
LpnPI CCDG 9 cut(s) 23, 69, 145, 184, 227, 357, 392, 487, 564
Lsp1109I GCAGC 2 cut(s) 179, 184
LweI GCATC 1 cut(s) 217
MaeI CTAG 2 cut(s) 263, 443
MaeII ACGT 3 cut(s) 45, 143, 525
MaeIII GTNAC 2 cut(s) 125, 374
MalI GATC 3 cut(s) 24, 215, 261
MbiI CCGCTC 1 cut(s) 440
MboI GATC 3 cut(s) 22, 213, 259
MboII GAAGA 2 cut(s) 32, 356
MflI RGATCY 2 cut(s) 22, 259
MhlI GDGCHC 2 cut(s) 316, 408
MluCI AATT 1 cut(s) 278
MlyI GAGTC 1 cut(s) 355
MmeI TCCRAC 1 cut(s) 191
MnlI CCTC 3 cut(s) 118, 285, 359
MspI CCGG 2 cut(s) 132, 474
MwoI GCNNNNNNNGC 5 cut(s) 47, 113, 176, 452, 584
NdeII GATC 3 cut(s) 22, 213, 259
NlaIII CATG 2 cut(s) 190, 600
NlaIV GGNNCC 1 cut(s) 32
NmuCI GTSAC 2 cut(s) 125, 374
PagI TCATGA 1 cut(s) 186
PcsI WCGNNNNNNNCGW 2 cut(s) 330, 522
PfeI GAWTC 2 cut(s) 95, 425
PflFI GACNNNGTC 1 cut(s) 524
PkrI GCNGC 2 cut(s) 169, 199
PleI GAGTC 1 cut(s) 355
PpsI GAGTC 1 cut(s) 355
PspEI GGTNACC 1 cut(s) 374
PspN4I GGNNCC 1 cut(s) 32
PspPI GGNCC 1 cut(s) 272
PsuI RGATCY 2 cut(s) 22, 259
PsyI GACNNNGTC 1 cut(s) 524
SalI GTCGAC 1 cut(s) 332
SatI GCNGC 2 cut(s) 168, 198
Sau3AI GATC 3 cut(s) 22, 213, 259
Sau96I GGNCC 1 cut(s) 272
SchI GAGTC 1 cut(s) 355
SduI GDGCHC 2 cut(s) 316, 408
SfaNI GCATC 1 cut(s) 217
SgrAI CRCCGGYG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 572
SmoI CTYRAG 1 cut(s) 572
Sse9I AATT 1 cut(s) 278
SsiI CCGC 2 cut(s) 123, 438
SspMI CTAG 2 cut(s) 263, 443
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 2 cut(s) 30, 481
TaiI ACGT 3 cut(s) 48, 146, 528
TaqI TCGA 3 cut(s) 18, 333, 516
TasI AATT 1 cut(s) 278
TfiI GAWTC 2 cut(s) 95, 425
TscAI CASTG 1 cut(s) 63
TseFI GTSAC 2 cut(s) 125, 374
TseI GCWGC 2 cut(s) 167, 197
Tsp45I GTSAC 2 cut(s) 125, 374
TspDTI ATGAA 1 cut(s) 175
TspRI CASTG 1 cut(s) 63
Tth111I GACNNNGTC 1 cut(s) 524
VneI GTGCAC 1 cut(s) 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XmiI GTMKAC 2 cut(s) 247, 333
XspI CTAG 2 cut(s) 263, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.