Rmu_sc0006168.1_g000025

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006168.1
N/A
Physical Location & Seq
Reverse (-)
73908 .. 78151
4244 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
atggcgacggcctctggcctagccaacgcaaggtgggtgcggccggtcgggatcagagtggactcggggctctgtcggggcatagcgatgtctggtgacggtcggaagtcgagctcagggtgccgtctagtatgggatagggatggcaatcagtggcgttcctcgccgagggtcagacggcaacgacgtcttttttgtccaacaacagtggtggatacggggatggtgacgcggcgaggctggcctagtccaggagggctgggtcccatacctgctagcttggggaaattatctttgctctacaggctggatctttccaataatcaattgaccgggtccattccagcgagtttgggaaatatgaagtttcaagcattattgttcgaccatctctttctgaatccttcttctcatcgccgcgtggatattgaattcacactggatctttcagacaatcaattgagtggatccataccagccactttagggaatttggccctctctttggtgatatctgatccagaagcctaccgactcgtcaagcttaagcaatatgacagtgcttatgtaatgtcatcgttctacctgagtctttcaaacaatcaactgactgggcctataccaaagagcttagggaatttgattttcgccgatactattgatctgcatggcaactttctcaacgcctctataccagcatctttgggggcggtgacaggacacatcacttctttgactgatgtacaaaaggggatacccttgggagtgcttgatccaaggaaggtgaaaaagggtcaactccattacaagattgcgaagccagaaaaggctggctctaaattttacagatacatgggctctctcacaacccctccttgtgctgaagaggttatttggcacataaacaaaaaggtacaaactgcttcgatagaacaagtgaagctacttcaagatgcagtttttgattttgcggcaaacaacgcaagaccattacaacctctcaacggccgaggaatcaagctctatggggactatcattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

346

Amino Acids

37.83

Weight (kDa)

9.83

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 275 - 340 1.7e-15 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 190
Acc36I ACCTGC 1 cut(s) 280
AccB1I GGYRCC 1 cut(s) 120
AccII CGCG 2 cut(s) 232, 420
AciI CCGC 5 cut(s) 40, 232, 418, 710, 971
AclWI GGATC 7 cut(s) 59, 318, 450, 462, 475, 512, 767
AcoI YGGCCR 2 cut(s) 41, 1006
AcsI RAATTY 4 cut(s) 431, 490, 637, 839
AcuI CTGAAG 1 cut(s) 903
AcyI GRCGYC 1 cut(s) 187
AfaI GTAC 2 cut(s) 744, 915
AfiI CCNNNNNNNGG 6 cut(s) 30, 168, 251, 486, 505, 1004
AflII CTTAAG 1 cut(s) 545
AgsI TTSAA 4 cut(s) 371, 431, 597, 950
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 6 cut(s) 114, 279, 544, 630, 943, 1021
AluI AGCT 6 cut(s) 114, 279, 544, 630, 943, 1021
Alw21I GWGCWC 1 cut(s) 116
AlwI GGATC 7 cut(s) 59, 318, 450, 462, 475, 512, 767
Ama87I CYCGRG 1 cut(s) 64
AoxI GGCC 7 cut(s) 9, 16, 41, 242, 495, 614, 1006
ApoI RAATTY 4 cut(s) 431, 490, 637, 839
ArsI GACNNNNNNTTYG 2 cut(s) 177, 209
AspS9I GGNCC 4 cut(s) 263, 336, 496, 614
AsuC2I CCSGG 1 cut(s) 334
AsuHPI GGTGA 5 cut(s) 107, 238, 520, 724, 796
AsuNHI GCTAGC 1 cut(s) 275
AvaI CYCGRG 1 cut(s) 64
AvaII GGWCC 2 cut(s) 263, 336
BamHI GGATCC 1 cut(s) 467
BanI GGYRCC 1 cut(s) 120
BanII GRGCYC 3 cut(s) 72, 116, 860
Bbv12I GWGCWC 1 cut(s) 116
BccI CCATC 3 cut(s) 137, 217, 396
BceAI ACGGC 4 cut(s) 24, 108, 194, 1021
BciT130I CCWGG 1 cut(s) 252
BciVI GTATCC 2 cut(s) 208, 747
BcnI CCSGG 1 cut(s) 334
BfaI CTAG 4 cut(s) 20, 128, 246, 276
BfmI CTRYAG 1 cut(s) 301
BfrI CTTAAG 1 cut(s) 545
BfuAI ACCTGC 1 cut(s) 280
BfuI GTATCC 2 cut(s) 208, 747
BisI GCNGC 4 cut(s) 41, 233, 418, 972
BlsI GCNGC 4 cut(s) 42, 234, 419, 973
Bme1390I CCNGG 2 cut(s) 252, 334
Bme18I GGWCC 2 cut(s) 263, 336
BmeT110I CYCGRG 1 cut(s) 64
BmgT120I GGNCC 4 cut(s) 263, 336, 496, 614
BmiI GGNNCC 5 cut(s) 122, 264, 265, 337, 469
BmrFI CCNGG 2 cut(s) 252, 334
BmrI ACTGGG 1 cut(s) 621
BmsI GCATC 2 cut(s) 707, 943
BmtI GCTAGC 1 cut(s) 279
BmuI ACTGGG 1 cut(s) 621
Bpu10I CCTNAGC 2 cut(s) 115, 631
BpuMI CCSGG 1 cut(s) 334
BsaBI GATNNNNATC 1 cut(s) 147
BsaHI GRCGYC 1 cut(s) 187
BsaJI CCNNGG 4 cut(s) 167, 759, 776, 1009
BsaXI ACNNNNNCTCC 2 cut(s) 856, 886
Bsc4I CCNNNNNNNGG 6 cut(s) 30, 168, 251, 486, 505, 1004
Bse118I RCCGGY 1 cut(s) 43
Bse1I ACTGG 2 cut(s) 444, 616
Bse8I GATNNNNATC 1 cut(s) 147
BseBI CCWGG 1 cut(s) 252
BseDI CCNNGG 4 cut(s) 167, 759, 776, 1009
BseGI GGATG 2 cut(s) 148, 228
BseJI GATNNNNATC 1 cut(s) 147
BseLI CCNNNNNNNGG 6 cut(s) 30, 168, 251, 486, 505, 1004
BseMII CTCAG 2 cut(s) 129, 578
BseNI ACTGG 2 cut(s) 444, 616
BseX3I CGGCCG 2 cut(s) 41, 1006
BseYI CCCAGC 1 cut(s) 259
Bsh1236I CGCG 2 cut(s) 232, 420
Bsh1285I CGRYCG 4 cut(s) 44, 48, 103, 1009
BshFI GGCC 7 cut(s) 11, 18, 43, 244, 497, 616, 1008
BshNI GGYRCC 1 cut(s) 120
BsiEI CGRYCG 4 cut(s) 44, 48, 103, 1009
BsiHKAI GWGCWC 1 cut(s) 116
BsiHKCI CYCGRG 1 cut(s) 64
BsiSI CCGG 2 cut(s) 44, 333
BslFI GGGAC 1 cut(s) 249
BslI CCNNNNNNNGG 6 cut(s) 30, 168, 251, 486, 505, 1004
BsmFI GGGAC 1 cut(s) 249
BsnI GGCC 7 cut(s) 11, 18, 43, 244, 497, 616, 1008
BsoBI CYCGRG 1 cut(s) 64
Bsp1286I GDGCHC 3 cut(s) 72, 116, 860
Bsp1407I TGTACA 1 cut(s) 742
Bsp143I GATC 7 cut(s) 51, 310, 442, 467, 517, 661, 772
BspACI CCGC 5 cut(s) 40, 232, 418, 710, 971
BspANI GGCC 7 cut(s) 11, 18, 43, 244, 497, 616, 1008
BspCNI CTCAG 2 cut(s) 128, 579
BspFNI CGCG 2 cut(s) 232, 420
BspLI GGNNCC 5 cut(s) 122, 264, 265, 337, 469
BspMI ACCTGC 1 cut(s) 280
BspOI GCTAGC 1 cut(s) 279
BspPI GGATC 7 cut(s) 59, 318, 450, 462, 475, 512, 767
BspT107I GGYRCC 1 cut(s) 120
BspTI CTTAAG 1 cut(s) 545
BsrFI RCCGGY 1 cut(s) 43
BsrGI TGTACA 1 cut(s) 742
BsrI ACTGG 2 cut(s) 444, 616
BssAI RCCGGY 1 cut(s) 43
BssECI CCNNGG 4 cut(s) 167, 759, 776, 1009
BssMI GATC 7 cut(s) 51, 310, 442, 467, 517, 661, 772
BssNI GRCGYC 1 cut(s) 187
BssT1I CCWWGG 2 cut(s) 759, 776
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 3 cut(s) 101, 208, 560
Bst6I CTCTTC 1 cut(s) 879
BstACI GRCGYC 1 cut(s) 187
BstAFI CTTAAG 1 cut(s) 545
BstAUI TGTACA 1 cut(s) 742
BstC8I GCNNGC 3 cut(s) 242, 277, 832
BstDEI CTNAG 3 cut(s) 115, 587, 631
BstENI CCTNNNNNAGG 2 cut(s) 166, 249
BstF5I GGATG 2 cut(s) 148, 228
BstFNI CGCG 2 cut(s) 232, 420
BstKTI GATC 7 cut(s) 54, 313, 445, 470, 520, 664, 775
BstMBI GATC 7 cut(s) 51, 310, 442, 467, 517, 661, 772
BstMCI CGRYCG 4 cut(s) 44, 48, 103, 1009
BstMWI GCNNNNNNNGC 5 cut(s) 120, 163, 241, 304, 980
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 2 cut(s) 250, 332
BstSFI CTRYAG 1 cut(s) 301
BstUI CGCG 2 cut(s) 232, 420
BstX2I RGATCY 3 cut(s) 310, 442, 467
BstYI RGATCY 3 cut(s) 310, 442, 467
BstZI CGGCCG 2 cut(s) 41, 1006
BsuI GTATCC 2 cut(s) 208, 747
BsuRI GGCC 7 cut(s) 11, 18, 43, 244, 497, 616, 1008
BtgZI GCGATG 2 cut(s) 101, 398
BtsCI GGATG 2 cut(s) 148, 228
BtsIMutI CAGTG 4 cut(s) 158, 213, 437, 565
BveI ACCTGC 1 cut(s) 280
Cac8I GCNNGC 3 cut(s) 242, 277, 832
Cfr10I RCCGGY 1 cut(s) 43
Cfr13I GGNCC 4 cut(s) 263, 336, 496, 614
CseI GACGC 1 cut(s) 238
Csp6I GTAC 2 cut(s) 743, 914
CspCI CAANNNNNGTGG 4 cut(s) 189, 192, 224, 227
CviAII CATG 2 cut(s) 668, 853
CviQI GTAC 2 cut(s) 743, 914
DdeI CTNAG 3 cut(s) 115, 587, 631
DpnI GATC 7 cut(s) 53, 312, 444, 469, 519, 663, 774
DpnII GATC 7 cut(s) 51, 310, 442, 467, 517, 661, 772
EaeI YGGCCR 2 cut(s) 41, 1006
EagI CGGCCG 2 cut(s) 41, 1006
Eam1104I CTCTTC 1 cut(s) 879
EarI CTCTTC 1 cut(s) 879
Ecl136II GAGCTC 1 cut(s) 114
EclXI CGGCCG 2 cut(s) 41, 1006
Eco130I CCWWGG 2 cut(s) 759, 776
Eco24I GRGCYC 3 cut(s) 72, 116, 860
Eco32I GATATC 1 cut(s) 513
Eco47I GGWCC 2 cut(s) 263, 336
Eco52I CGGCCG 2 cut(s) 41, 1006
Eco53kI GAGCTC 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 903
Eco88I CYCGRG 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 114
EcoNI CCTNNNNNAGG 2 cut(s) 166, 249
EcoO109I RGGNCCY 1 cut(s) 263
EcoRI GAATTC 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 250
EcoRV GATATC 1 cut(s) 513
EcoT14I CCWWGG 2 cut(s) 759, 776
EcoT38I GRGCYC 3 cut(s) 72, 116, 860
ErhI CCWWGG 2 cut(s) 759, 776
FaeI CATG 2 cut(s) 671, 856
FaqI GGGAC 1 cut(s) 249
FatI CATG 2 cut(s) 667, 852
Fnu4HI GCNGC 4 cut(s) 41, 233, 418, 972
FokI GGATG 2 cut(s) 155, 235
FriOI GRGCYC 3 cut(s) 72, 116, 860
Fsp4HI GCNGC 4 cut(s) 41, 233, 418, 972
FspBI CTAG 4 cut(s) 20, 128, 246, 276
GluI GCNGC 4 cut(s) 41, 233, 418, 972
GsaI CCCAGC 1 cut(s) 263
HaeIII GGCC 7 cut(s) 11, 18, 43, 244, 497, 616, 1008
HapII CCGG 2 cut(s) 44, 333
HgaI GACGC 1 cut(s) 238
Hin1I GRCGYC 1 cut(s) 187
Hin1II CATG 2 cut(s) 671, 856
HincII GTYRAC 1 cut(s) 797
HindII GTYRAC 1 cut(s) 797
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 5 cut(s) 62, 400, 534, 589, 1014
HpaII CCGG 2 cut(s) 44, 333
HphI GGTGA 5 cut(s) 107, 238, 520, 724, 796
Hpy166II GTNNAC 2 cut(s) 61, 797
Hpy188I TCNGA 6 cut(s) 56, 105, 176, 399, 451, 517
Hpy188III TCNNGA 3 cut(s) 49, 521, 950
Hpy8I GTNNAC 2 cut(s) 61, 797
Hpy99I CGWCG 2 cut(s) 10, 189
HpyAV CCTTC 2 cut(s) 414, 775
HpyCH4III ACNGT 3 cut(s) 101, 208, 560
HpyCH4IV ACGT 1 cut(s) 187
HpyCH4V TGCA 2 cut(s) 667, 956
HpyF10VI GCNNNNNNNGC 5 cut(s) 120, 163, 241, 304, 980
HpyF3I CTNAG 3 cut(s) 115, 587, 631
HpySE526I ACGT 1 cut(s) 187
Hsp92I GRCGYC 1 cut(s) 187
Hsp92II CATG 2 cut(s) 671, 856
KflI GGGWCCC 1 cut(s) 263
Kzo9I GATC 7 cut(s) 51, 310, 442, 467, 517, 661, 772
LweI GCATC 2 cut(s) 707, 943
MaeI CTAG 4 cut(s) 20, 128, 246, 276
MaeII ACGT 1 cut(s) 187
MaeIII GTNAC 3 cut(s) 95, 226, 712
MalI GATC 7 cut(s) 53, 312, 444, 469, 519, 663, 774
MboI GATC 7 cut(s) 51, 310, 442, 467, 517, 661, 772
MboII GAAGA 2 cut(s) 399, 896
MfeI CAATTG 2 cut(s) 326, 458
MflI RGATCY 3 cut(s) 310, 442, 467
MhlI GDGCHC 3 cut(s) 72, 116, 860
MluCI AATT 7 cut(s) 287, 326, 431, 458, 490, 637, 839
MlyI GAGTC 3 cut(s) 56, 528, 598
MmeI TCCRAC 2 cut(s) 83, 224
MseI TTAA 1 cut(s) 546
MslI CAYNNNNRTG 1 cut(s) 86
MspCI CTTAAG 1 cut(s) 545
MspI CCGG 2 cut(s) 44, 333
MspR9I CCNGG 2 cut(s) 252, 334
MunI CAATTG 2 cut(s) 326, 458
MvaI CCWGG 1 cut(s) 252
MvnI CGCG 2 cut(s) 232, 420
MwoI GCNNNNNNNGC 5 cut(s) 120, 163, 241, 304, 980
NciI CCSGG 1 cut(s) 334
NdeII GATC 7 cut(s) 51, 310, 442, 467, 517, 661, 772
NheI GCTAGC 1 cut(s) 275
NlaIII CATG 2 cut(s) 671, 856
NlaIV GGNNCC 5 cut(s) 122, 264, 265, 337, 469
NmeAIII GCCGAG 2 cut(s) 192, 1034
NmuCI GTSAC 3 cut(s) 95, 226, 712
PcsI WCGNNNNNNNCGW 1 cut(s) 184
PfeI GAWTC 2 cut(s) 400, 1014
PflFI GACNNNGTC 1 cut(s) 334
PfoI TCCNGGA 1 cut(s) 250
PkrI GCNGC 4 cut(s) 42, 234, 419, 973
PleI GAGTC 3 cut(s) 56, 528, 597
PpsI GAGTC 3 cut(s) 56, 528, 597
PpuMI RGGWCCY 1 cut(s) 263
Psp124BI GAGCTC 1 cut(s) 116
Psp5II RGGWCCY 1 cut(s) 263
Psp6I CCWGG 1 cut(s) 250
PspFI CCCAGC 1 cut(s) 259
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 5 cut(s) 122, 264, 265, 337, 469
PspPI GGNCC 4 cut(s) 263, 336, 496, 614
PspPPI RGGWCCY 1 cut(s) 263
PsuI RGATCY 3 cut(s) 310, 442, 467
PsyI GACNNNGTC 1 cut(s) 334
RsaI GTAC 2 cut(s) 744, 915
RsaNI GTAC 2 cut(s) 743, 914
RseI CAYNNNNRTG 1 cut(s) 86
SacI GAGCTC 1 cut(s) 116
SaqAI TTAA 1 cut(s) 546
SatI GCNGC 4 cut(s) 41, 233, 418, 972
Sau3AI GATC 7 cut(s) 51, 310, 442, 467, 517, 661, 772
Sau96I GGNCC 4 cut(s) 263, 336, 496, 614
SchI GAGTC 3 cut(s) 56, 528, 598
ScrFI CCNGG 2 cut(s) 252, 334
SduI GDGCHC 3 cut(s) 72, 116, 860
SfaNI GCATC 2 cut(s) 707, 943
SfcI CTRYAG 1 cut(s) 301
SinI GGWCC 2 cut(s) 263, 336
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 7 cut(s) 287, 326, 431, 458, 490, 637, 839
SsiI CCGC 5 cut(s) 40, 232, 418, 710, 971
SspMI CTAG 4 cut(s) 20, 128, 246, 276
SstI GAGCTC 1 cut(s) 116
StyD4I CCNGG 2 cut(s) 250, 332
StyI CCWWGG 2 cut(s) 759, 776
TaaI ACNGT 3 cut(s) 101, 208, 560
TaiI ACGT 1 cut(s) 190
TaqI TCGA 3 cut(s) 110, 384, 926
TasI AATT 7 cut(s) 287, 326, 431, 458, 490, 637, 839
TatI WGTACW 1 cut(s) 742
TauI GCSGC 4 cut(s) 43, 235, 420, 974
TfiI GAWTC 2 cut(s) 400, 1014
Tru1I TTAA 1 cut(s) 546
Tru9I TTAA 1 cut(s) 546
TscAI CASTG 4 cut(s) 158, 213, 444, 565
TseFI GTSAC 3 cut(s) 95, 226, 712
Tsp45I GTSAC 3 cut(s) 95, 226, 712
TspDTI ATGAA 1 cut(s) 377
TspRI CASTG 4 cut(s) 158, 213, 444, 565
Tth111I GACNNNGTC 1 cut(s) 334
Vha464I CTTAAG 1 cut(s) 545
VpaK11BI GGWCC 2 cut(s) 263, 336
XagI CCTNNNNNAGG 2 cut(s) 166, 249
XapI RAATTY 4 cut(s) 431, 490, 637, 839
XspI CTAG 4 cut(s) 20, 128, 246, 276
ZraI GACGTC 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.