Rmu_sc0006175.1_g000020

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006175.1
N/A
Physical Location & Seq
Forward (+)
61465 .. 63594
2130 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 861 bp
atgcagcggatgtatgatgcttcgagagggaagatatctaaacacaaagcccctgagcaaatcaaggcaataagccagaaattatctacattagctttggaagaggagaagcaatctgttagtaaacatttcatgaagaagaagaagaaggaggagaggaggaagaagaagaagaaggagaagaagaagtccaggtggaggaggcaacaagaagtgcgtaggaagaatgatcttgagatgttggctgcttacaaaaagcggcaagaagagaaagagaaagaagaggaggaggaggaggaggaggaggaggagcagacagaagaagagatggaggaagaggaactggaagagggaccggaagagaaacagaaacggattgagaaagagaaagagatagagcaacagaaacagaaacggaaagaggaattggctgcttttttggcaagggaaagggaagaggccgctgagagtatgcctatgtcatccaccataaacacatcggcaggcgctttgtataaggtgttttgtgcatcagctgatccagcaagacaagaaatgcatgaagcgtgtttcggctacttgagccttggaggcatctgttcatcatatggaccagtatctcttctctcaggtcttaacccttgcccactgcacttgtttctccttttctttgctgttgctatctatggtgtagggcgcttaatgcttccattcccttcacccaaacgcaagtggcttggggctagattgatctcgagtgcatcagacatcatattccccattataaagggtgaaggagttagacagatgttttttcctgccacagtgccagcatactacagatctcctcctttttattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

286

Amino Acids

33.31

Weight (kDa)

8.77

Isoelectric Point (pI)

90.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SE PF08491 159 - 239 1.5e-23 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 785
AciI CCGC 3 cut(s) 7, 259, 462
AclWI GGATC 1 cut(s) 533
AfiI CCNNNNNNNGG 1 cut(s) 198
AjnI CCWGG 1 cut(s) 191
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AluBI AGCT 2 cut(s) 95, 536
AluI AGCT 2 cut(s) 95, 536
AlwI GGATC 1 cut(s) 533
Ama87I CYCGRG 1 cut(s) 754
AoxI GGCC 1 cut(s) 459
ApeKI GCWGC 3 cut(s) 4, 245, 431
AspLEI GCGC 2 cut(s) 509, 699
AspS9I GGNCC 2 cut(s) 353, 611
AsuHPI GGTGA 2 cut(s) 711, 803
AvaI CYCGRG 1 cut(s) 754
AvaII GGWCC 2 cut(s) 353, 611
BbvI GCAGC 3 cut(s) 16, 232, 418
BccI CCATC 1 cut(s) 322
BciT130I CCWGG 1 cut(s) 193
BfaI CTAG 1 cut(s) 744
BfmI CTRYAG 1 cut(s) 838
BfoI RGCGCY 2 cut(s) 510, 700
BglI GCCNNNNNGGC 1 cut(s) 591
BglII AGATCT 1 cut(s) 842
BisI GCNGC 5 cut(s) 5, 246, 260, 432, 462
BlsI GCNGC 5 cut(s) 6, 247, 261, 433, 463
Bme1390I CCNGG 1 cut(s) 193
Bme18I GGWCC 2 cut(s) 353, 611
BmeT110I CYCGRG 1 cut(s) 754
BmgT120I GGNCC 2 cut(s) 353, 611
BmiI GGNNCC 1 cut(s) 354
BmrFI CCNGG 1 cut(s) 193
BmsI GCATC 4 cut(s) 7, 539, 603, 770
Bpu10I CCTNAGC 1 cut(s) 54
BpuEI CTTGAG 2 cut(s) 254, 601
BsaJI CCNNGG 1 cut(s) 586
BsaWI WCCGGW 1 cut(s) 355
Bsc4I CCNNNNNNNGG 1 cut(s) 198
Bse1I ACTGG 2 cut(s) 348, 614
BseBI CCWGG 1 cut(s) 193
BseDI CCNNGG 1 cut(s) 586
BseGI GGATG 2 cut(s) 15, 482
BseLI CCNNNNNNNGG 1 cut(s) 198
BseMII CTCAG 3 cut(s) 45, 456, 642
BseNI ACTGG 2 cut(s) 348, 614
BseXI GCAGC 3 cut(s) 16, 232, 418
BsgI GTGCAG 1 cut(s) 635
BshFI GGCC 1 cut(s) 461
BsiHKCI CYCGRG 1 cut(s) 754
BsiSI CCGG 1 cut(s) 356
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 1 cut(s) 198
BsmFI GGGAC 1 cut(s) 366
BsnI GGCC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 754
Bsp143I GATC 4 cut(s) 229, 538, 750, 842
BspACI CCGC 3 cut(s) 7, 259, 462
BspANI GGCC 1 cut(s) 461
BspCNI CTCAG 3 cut(s) 46, 457, 641
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 354
BspPI GGATC 1 cut(s) 533
BsrI ACTGG 2 cut(s) 348, 614
BssECI CCNNGG 1 cut(s) 586
BssMI GATC 4 cut(s) 229, 538, 750, 842
BssT1I CCWWGG 1 cut(s) 586
Bst2UI CCWGG 1 cut(s) 193
Bst4CI ACNGT 1 cut(s) 826
Bst6I CTCTTC 9 cut(s) 96, 261, 276, 318, 330, 342, 354, 450, 627
BstC8I GCNNGC 2 cut(s) 505, 831
BstDEI CTNAG 3 cut(s) 54, 465, 628
BstF5I GGATG 2 cut(s) 15, 482
BstH2I RGCGCY 2 cut(s) 510, 700
BstHHI GCGC 2 cut(s) 509, 699
BstKTI GATC 4 cut(s) 232, 541, 753, 845
BstMBI GATC 4 cut(s) 229, 538, 750, 842
BstMWI GCNNNNNNNGC 5 cut(s) 440, 542, 582, 591, 703
BstNI CCWGG 1 cut(s) 193
BstSCI CCNGG 1 cut(s) 191
BstSFI CTRYAG 1 cut(s) 838
BstV1I GCAGC 3 cut(s) 16, 232, 418
BstX2I RGATCY 1 cut(s) 842
BstYI RGATCY 1 cut(s) 842
BsuRI GGCC 1 cut(s) 461
BtsCI GGATG 2 cut(s) 15, 482
BtsI GCAGTG 1 cut(s) 647
BtsIMutI CAGTG 2 cut(s) 647, 831
Cac8I GCNNGC 2 cut(s) 505, 831
CciI TCATGA 1 cut(s) 132
CfoI GCGC 2 cut(s) 509, 699
Cfr13I GGNCC 2 cut(s) 353, 611
CspCI CAANNNNNGTGG 2 cut(s) 636, 671
CviAII CATG 2 cut(s) 133, 560
DdeI CTNAG 3 cut(s) 54, 465, 628
DpnI GATC 4 cut(s) 231, 540, 752, 844
DpnII GATC 4 cut(s) 229, 538, 750, 842
Eam1104I CTCTTC 9 cut(s) 96, 261, 276, 318, 330, 342, 354, 450, 627
EarI CTCTTC 9 cut(s) 96, 261, 276, 318, 330, 342, 354, 450, 627
Eco130I CCWWGG 1 cut(s) 586
Eco32I GATATC 1 cut(s) 36
Eco47I GGWCC 2 cut(s) 353, 611
Eco88I CYCGRG 1 cut(s) 754
EcoRII CCWGG 1 cut(s) 191
EcoRV GATATC 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 586
EcoT22I ATGCAT 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 586
FaeI CATG 2 cut(s) 136, 563
FaqI GGGAC 1 cut(s) 366
FatI CATG 2 cut(s) 132, 559
FauNDI CATATG 1 cut(s) 607
Fnu4HI GCNGC 5 cut(s) 5, 246, 260, 432, 462
FokI GGATG 2 cut(s) 22, 469
Fsp4HI GCNGC 5 cut(s) 5, 246, 260, 432, 462
FspBI CTAG 1 cut(s) 744
GlaI GCGC 2 cut(s) 508, 698
GluI GCNGC 5 cut(s) 5, 246, 260, 432, 462
HaeII RGCGCY 2 cut(s) 510, 700
HaeIII GGCC 1 cut(s) 461
HapII CCGG 1 cut(s) 356
HhaI GCGC 2 cut(s) 509, 699
Hin1II CATG 2 cut(s) 136, 563
Hin6I GCGC 2 cut(s) 507, 697
HinP1I GCGC 2 cut(s) 507, 697
HpaII CCGG 1 cut(s) 356
HphI GGTGA 2 cut(s) 711, 803
Hpy166II GTNNAC 1 cut(s) 125
Hpy188I TCNGA 1 cut(s) 766
Hpy188III TCNNGA 4 cut(s) 24, 133, 233, 754
Hpy8I GTNNAC 1 cut(s) 125
HpyAV CCTTC 4 cut(s) 142, 169, 726, 788
HpyCH4III ACNGT 1 cut(s) 826
HpyCH4V TGCA 5 cut(s) 4, 530, 559, 652, 761
HpyF10VI GCNNNNNNNGC 5 cut(s) 440, 542, 582, 591, 703
HpyF3I CTNAG 3 cut(s) 54, 465, 628
Hsp92II CATG 2 cut(s) 136, 563
HspAI GCGC 2 cut(s) 507, 697
Kzo9I GATC 4 cut(s) 229, 538, 750, 842
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 3 cut(s) 16, 232, 418
LweI GCATC 4 cut(s) 7, 539, 603, 770
MaeI CTAG 1 cut(s) 744
MalI GATC 4 cut(s) 231, 540, 752, 844
MboI GATC 4 cut(s) 229, 538, 750, 842
MflI RGATCY 1 cut(s) 842
MluCI AATT 2 cut(s) 80, 425
Mph1103I ATGCAT 1 cut(s) 561
MseI TTAA 2 cut(s) 636, 701
MspA1I CMGCKG 3 cut(s) 7, 464, 536
MspI CCGG 1 cut(s) 356
MspR9I CCNGG 1 cut(s) 193
MvaI CCWGG 1 cut(s) 193
MwoI GCNNNNNNNGC 5 cut(s) 440, 542, 582, 591, 703
NdeI CATATG 1 cut(s) 607
NdeII GATC 4 cut(s) 229, 538, 750, 842
NlaIII CATG 2 cut(s) 136, 563
NlaIV GGNNCC 1 cut(s) 354
NsiI ATGCAT 1 cut(s) 561
PaeR7I CTCGAG 1 cut(s) 754
PagI TCATGA 1 cut(s) 132
PkrI GCNGC 5 cut(s) 6, 247, 261, 433, 463
PsiI TTATAA 1 cut(s) 785
Psp6I CCWGG 1 cut(s) 191
PspGI CCWGG 1 cut(s) 191
PspN4I GGNNCC 1 cut(s) 354
PspPI GGNCC 2 cut(s) 353, 611
PsuI RGATCY 1 cut(s) 842
PvuII CAGCTG 1 cut(s) 536
SaqAI TTAA 2 cut(s) 636, 701
SatI GCNGC 5 cut(s) 5, 246, 260, 432, 462
Sau3AI GATC 4 cut(s) 229, 538, 750, 842
Sau96I GGNCC 2 cut(s) 353, 611
ScrFI CCNGG 1 cut(s) 193
SetI ASST 5 cut(s) 97, 197, 522, 538, 634
SfaNI GCATC 4 cut(s) 7, 539, 603, 770
SfcI CTRYAG 1 cut(s) 838
Sfr274I CTCGAG 1 cut(s) 754
SinI GGWCC 2 cut(s) 353, 611
SlaI CTCGAG 1 cut(s) 754
SmlI CTYRAG 3 cut(s) 233, 580, 754
SmoI CTYRAG 3 cut(s) 233, 580, 754
Sse9I AATT 2 cut(s) 80, 425
SsiI CCGC 3 cut(s) 7, 259, 462
SspMI CTAG 1 cut(s) 744
StyD4I CCNGG 1 cut(s) 191
StyI CCWWGG 1 cut(s) 586
TaaI ACNGT 1 cut(s) 826
TaqI TCGA 2 cut(s) 23, 755
TasI AATT 2 cut(s) 80, 425
TauI GCSGC 2 cut(s) 262, 464
Tru1I TTAA 2 cut(s) 636, 701
Tru9I TTAA 2 cut(s) 636, 701
TscAI CASTG 2 cut(s) 654, 831
TseI GCWGC 3 cut(s) 4, 245, 431
TspDTI ATGAA 4 cut(s) 121, 149, 576, 591
TspGWI ACGGA 2 cut(s) 388, 430
TspRI CASTG 2 cut(s) 654, 831
VpaK11BI GGWCC 2 cut(s) 353, 611
XhoI CTCGAG 1 cut(s) 754
XspI CTAG 1 cut(s) 744
Zsp2I ATGCAT 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.