Rmu_sc0006236.1_g000005

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006236.1
N/A
Physical Location & Seq
Reverse (-)
20862 .. 23020
2159 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
atggtggctgctttgaatcagaatttggaggaccgatctaaagttgggcgcattatcgatgattataagtcatatttgacatcattacattcaattaagatttctcatgtcttccgggaagcaaatggtgtagctaatagattagcttatcttgctagttcaaattttcttaatgatgtatggttagatgaggctcctgttattattcagaatgtcctctatgaggatttttctgcgtggtctcgtccggcggtgccctggctctggcgtgggcctctggtctcaccggtgcatggtggctgtttccggacggggatttggtgtcttcgaggtggtggaggcggaagcgttgcagtcgactttcctctgccttgcttgaggtcggcggctttgctaacggtggtttcggtctgggtcgagtggtgcagatctttgtccggtgcaggagcgttcgacgctgggtgggtaggcggtgttgttgtgcagcgctgttgggtatggtctgcggtggtaggggtcgatgggaagcggcggagcaggtgggttcttggaggagttttttttcaggccgcgttgttctattggcgaggaggatggttgagatggctaggtgttgacttttggggatcaccacagatcagatctgcagcggggtggagcgacaaagccaacttcatctcagcgacggcggccgttatgtatgatccggtgattgtagcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box WD40

Protein Analysis

240

Amino Acids

26.45

Weight (kDa)

9.09

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 66
AarI CACCTGC 1 cut(s) 528
Acc36I ACCTGC 1 cut(s) 528
AccB1I GGYRCC 1 cut(s) 253
AccI GTMKAC 1 cut(s) 357
AccII CGCG 1 cut(s) 572
AccIII TCCGGA 1 cut(s) 306
AclWI GGATC 2 cut(s) 634, 698
AcoI YGGCCR 1 cut(s) 690
AcsI RAATTY 2 cut(s) 22, 163
AfeI AGCGCT 1 cut(s) 488
AfiI CCNNNNNNNGG 3 cut(s) 223, 264, 293
AgeI ACCGGT 1 cut(s) 286
AgsI TTSAA 3 cut(s) 16, 93, 162
AjnI CCWGG 1 cut(s) 257
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AluBI AGCT 3 cut(s) 134, 146, 719
AluI AGCT 3 cut(s) 134, 146, 719
Alw26I GTCTC 2 cut(s) 246, 286
AlwI GGATC 2 cut(s) 634, 698
Aor13HI TCCGGA 1 cut(s) 306
Aor51HI AGCGCT 1 cut(s) 488
AoxI GGCC 3 cut(s) 272, 567, 690
ApeKI GCWGC 3 cut(s) 8, 484, 647
ApoI RAATTY 2 cut(s) 22, 163
AsiGI ACCGGT 1 cut(s) 286
AspLEI GCGC 2 cut(s) 51, 489
AspS9I GGNCC 2 cut(s) 31, 272
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 3 cut(s) 276, 621, 721
AvaII GGWCC 1 cut(s) 31
BaeGI GKGCMC 1 cut(s) 258
BanI GGYRCC 1 cut(s) 253
BbsI GAAGAC 2 cut(s) 103, 317
BbvI GCAGC 2 cut(s) 496, 659
BccI CCATC 3 cut(s) 515, 588, 597
BceAI ACGGC 2 cut(s) 677, 702
BciT130I CCWGG 1 cut(s) 259
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 2 cut(s) 246, 286
BfaI CTAG 2 cut(s) 156, 608
BfmI CTRYAG 1 cut(s) 645
BfoI RGCGCY 1 cut(s) 490
BfuAI ACCTGC 1 cut(s) 528
BglII AGATCT 2 cut(s) 428, 641
BisI GCNGC 7 cut(s) 9, 387, 485, 530, 570, 648, 690
BlsI GCNGC 7 cut(s) 10, 388, 486, 531, 571, 649, 691
Bme1390I CCNGG 2 cut(s) 116, 259
Bme18I GGWCC 1 cut(s) 31
BmgT120I GGNCC 2 cut(s) 31, 272
BmiI GGNNCC 2 cut(s) 195, 255
BmrFI CCNGG 2 cut(s) 116, 259
BpiI GAAGAC 2 cut(s) 103, 317
BpuEI CTTGAG 1 cut(s) 397
BpuMI CCSGG 1 cut(s) 116
Bsa29I ATCGAT 1 cut(s) 57
BsaI GGTCTC 2 cut(s) 246, 286
BsaJI CCNNGG 1 cut(s) 257
BsaWI WCCGGW 4 cut(s) 286, 306, 437, 706
Bsc4I CCNNNNNNNGG 3 cut(s) 223, 264, 293
Bse118I RCCGGY 1 cut(s) 286
BseAI TCCGGA 1 cut(s) 306
BseBI CCWGG 1 cut(s) 259
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 1 cut(s) 257
BseGI GGATG 1 cut(s) 599
BseLI CCNNNNNNNGG 3 cut(s) 223, 264, 293
BseMII CTCAG 1 cut(s) 693
BseRI GAGGAG 2 cut(s) 567, 603
BseSI GKGCMC 1 cut(s) 258
BseX3I CGGCCG 1 cut(s) 690
BseXI GCAGC 2 cut(s) 496, 659
BseYI CCCAGC 1 cut(s) 458
BsgI GTGCAG 3 cut(s) 445, 462, 503
Bsh1236I CGCG 1 cut(s) 572
Bsh1285I CGRYCG 1 cut(s) 693
BshFI GGCC 3 cut(s) 274, 569, 692
BshNI GGYRCC 1 cut(s) 253
BshTI ACCGGT 1 cut(s) 286
BshVI ATCGAT 1 cut(s) 57
BsiEI CGRYCG 1 cut(s) 693
BsiSI CCGG 6 cut(s) 115, 248, 287, 307, 438, 707
BslI CCNNNNNNNGG 3 cut(s) 223, 264, 293
BsmAI GTCTC 2 cut(s) 246, 286
BsnI GGCC 3 cut(s) 274, 569, 692
Bso31I GGTCTC 2 cut(s) 246, 286
Bsp1286I GDGCHC 1 cut(s) 258
Bsp13I TCCGGA 1 cut(s) 306
Bsp143I GATC 6 cut(s) 35, 428, 626, 636, 641, 703
BspANI GGCC 3 cut(s) 274, 569, 692
BspCNI CTCAG 1 cut(s) 692
BspDI ATCGAT 1 cut(s) 57
BspEI TCCGGA 1 cut(s) 306
BspFNI CGCG 1 cut(s) 572
BspLI GGNNCC 2 cut(s) 195, 255
BspMAI CTGCAG 1 cut(s) 649
BspMI ACCTGC 1 cut(s) 528
BspPI GGATC 2 cut(s) 634, 698
BspT107I GGYRCC 1 cut(s) 253
BspTNI GGTCTC 2 cut(s) 246, 286
BsrFI RCCGGY 1 cut(s) 286
BssAI RCCGGY 1 cut(s) 286
BssECI CCNNGG 1 cut(s) 257
BssMI GATC 6 cut(s) 35, 428, 626, 636, 641, 703
Bst2UI CCWGG 1 cut(s) 259
Bst4CI ACNGT 1 cut(s) 400
BstDEI CTNAG 2 cut(s) 679, 720
BstENI CCTNNNNNAGG 1 cut(s) 221
BstF5I GGATG 1 cut(s) 599
BstFNI CGCG 1 cut(s) 572
BstH2I RGCGCY 1 cut(s) 490
BstHHI GCGC 2 cut(s) 51, 489
BstKTI GATC 6 cut(s) 38, 431, 629, 639, 644, 706
BstMAI GTCTC 2 cut(s) 246, 286
BstMBI GATC 6 cut(s) 35, 428, 626, 636, 641, 703
BstMCI CGRYCG 1 cut(s) 693
BstMWI GCNNNNNNNGC 3 cut(s) 152, 455, 689
BstNI CCWGG 1 cut(s) 259
BstSCI CCNGG 2 cut(s) 114, 257
BstSFI CTRYAG 1 cut(s) 645
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 1 cut(s) 572
BstV1I GCAGC 2 cut(s) 496, 659
BstV2I GAAGAC 2 cut(s) 103, 317
BstX2I RGATCY 2 cut(s) 428, 641
BstYI RGATCY 2 cut(s) 428, 641
BstZI CGGCCG 1 cut(s) 690
Bsu15I ATCGAT 1 cut(s) 57
BsuRI GGCC 3 cut(s) 274, 569, 692
BsuTUI ATCGAT 1 cut(s) 57
BtsCI GGATG 1 cut(s) 599
BveI ACCTGC 1 cut(s) 528
CfoI GCGC 2 cut(s) 51, 489
Cfr10I RCCGGY 1 cut(s) 286
Cfr13I GGNCC 2 cut(s) 31, 272
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 1 cut(s) 464
CspAI ACCGGT 1 cut(s) 286
CviAII CATG 2 cut(s) 107, 293
DdeI CTNAG 2 cut(s) 679, 720
DpnI GATC 6 cut(s) 37, 430, 628, 638, 643, 705
DpnII GATC 6 cut(s) 35, 428, 626, 636, 641, 703
EaeI YGGCCR 1 cut(s) 690
EagI CGGCCG 1 cut(s) 690
EciI GGCGGA 2 cut(s) 357, 547
EclXI CGGCCG 1 cut(s) 690
Eco31I GGTCTC 2 cut(s) 246, 286
Eco47I GGWCC 1 cut(s) 31
Eco47III AGCGCT 1 cut(s) 488
Eco52I CGGCCG 1 cut(s) 690
EcoNI CCTNNNNNAGG 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 257
FaeI CATG 2 cut(s) 110, 296
FaiI YATR 9 cut(s) 66, 73, 108, 181, 222, 294, 499, 698, 702
FatI CATG 2 cut(s) 106, 292
FauI CCCGC 1 cut(s) 643
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 7 cut(s) 9, 387, 485, 530, 570, 648, 690
FokI GGATG 1 cut(s) 606
Fsp4HI GCNGC 7 cut(s) 9, 387, 485, 530, 570, 648, 690
FspBI CTAG 2 cut(s) 156, 608
GlaI GCGC 2 cut(s) 50, 488
GluI GCNGC 7 cut(s) 9, 387, 485, 530, 570, 648, 690
GsaI CCCAGC 1 cut(s) 462
HaeII RGCGCY 1 cut(s) 490
HaeIII GGCC 3 cut(s) 274, 569, 692
HapII CCGG 6 cut(s) 115, 248, 287, 307, 438, 707
HgaI GACGC 1 cut(s) 464
HhaI GCGC 2 cut(s) 51, 489
Hin1II CATG 2 cut(s) 110, 296
Hin6I GCGC 2 cut(s) 49, 487
HinP1I GCGC 2 cut(s) 49, 487
HincII GTYRAC 2 cut(s) 358, 616
HindII GTYRAC 2 cut(s) 358, 616
HinfI GANTC 1 cut(s) 16
HpaII CCGG 6 cut(s) 115, 248, 287, 307, 438, 707
HphI GGTGA 3 cut(s) 276, 621, 721
Hpy166II GTNNAC 2 cut(s) 358, 616
Hpy188I TCNGA 3 cut(s) 21, 210, 641
Hpy188III TCNNGA 1 cut(s) 307
Hpy8I GTNNAC 2 cut(s) 358, 616
Hpy99I CGWCG 2 cut(s) 458, 688
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4V TGCA 6 cut(s) 292, 353, 426, 443, 484, 647
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 455, 689
HpyF3I CTNAG 2 cut(s) 679, 720
Hsp92II CATG 2 cut(s) 110, 296
HspAI GCGC 2 cut(s) 49, 487
Kpn2I TCCGGA 1 cut(s) 306
Kzo9I GATC 6 cut(s) 35, 428, 626, 636, 641, 703
LmnI GCTCC 4 cut(s) 199, 446, 534, 657
Lsp1109I GCAGC 2 cut(s) 496, 659
MaeI CTAG 2 cut(s) 156, 608
MalI GATC 6 cut(s) 37, 430, 628, 638, 643, 705
MboI GATC 6 cut(s) 35, 428, 626, 636, 641, 703
MboII GAAGA 2 cut(s) 103, 317
MflI RGATCY 2 cut(s) 428, 641
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 3 cut(s) 22, 93, 163
MroI TCCGGA 1 cut(s) 306
MseI TTAA 2 cut(s) 96, 171
MspA1I CMGCKG 1 cut(s) 650
MspI CCGG 6 cut(s) 115, 248, 287, 307, 438, 707
MspR9I CCNGG 2 cut(s) 116, 259
MvaI CCWGG 1 cut(s) 259
MvnI CGCG 1 cut(s) 572
MwoI GCNNNNNNNGC 3 cut(s) 152, 455, 689
NciI CCSGG 1 cut(s) 116
NdeII GATC 6 cut(s) 35, 428, 626, 636, 641, 703
NlaIII CATG 2 cut(s) 110, 296
NlaIV GGNNCC 2 cut(s) 195, 255
PaqCI CACCTGC 1 cut(s) 528
PfeI GAWTC 1 cut(s) 16
PfoI TCCNGGA 1 cut(s) 114
PinAI ACCGGT 1 cut(s) 286
PkrI GCNGC 7 cut(s) 10, 388, 486, 531, 571, 649, 691
PsiI TTATAA 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 257
PspFI CCCAGC 1 cut(s) 458
PspGI CCWGG 1 cut(s) 257
PspN4I GGNNCC 2 cut(s) 195, 255
PspPI GGNCC 2 cut(s) 31, 272
PstI CTGCAG 1 cut(s) 649
PsuI RGATCY 2 cut(s) 428, 641
SalI GTCGAC 1 cut(s) 356
SaqAI TTAA 2 cut(s) 96, 171
SatI GCNGC 7 cut(s) 9, 387, 485, 530, 570, 648, 690
Sau3AI GATC 6 cut(s) 35, 428, 626, 636, 641, 703
Sau96I GGNCC 2 cut(s) 31, 272
ScrFI CCNGG 2 cut(s) 116, 259
SduI GDGCHC 1 cut(s) 258
SetI ASST 7 cut(s) 136, 148, 334, 383, 542, 613, 721
SfcI CTRYAG 1 cut(s) 645
SgrAI CRCCGGYG 1 cut(s) 286
SinI GGWCC 1 cut(s) 31
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
Sse9I AATT 3 cut(s) 22, 93, 163
SspMI CTAG 2 cut(s) 156, 608
StyD4I CCNGG 2 cut(s) 114, 257
TaaI ACNGT 1 cut(s) 400
TaqI TCGA 6 cut(s) 57, 328, 357, 417, 453, 519
TaqII GACCGA 2 cut(s) 48, 397
TasI AATT 3 cut(s) 22, 93, 163
TauI GCSGC 4 cut(s) 389, 532, 572, 692
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 2 cut(s) 96, 171
Tru9I TTAA 2 cut(s) 96, 171
TseI GCWGC 3 cut(s) 8, 484, 647
TspDTI ATGAA 1 cut(s) 664
VpaK11BI GGWCC 1 cut(s) 31
XagI CCTNNNNNAGG 1 cut(s) 221
XapI RAATTY 2 cut(s) 22, 163
XmiI GTMKAC 1 cut(s) 357
XspI CTAG 2 cut(s) 156, 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.