Rmu_sc0006236.1_g000009

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006236.1
N/A
Physical Location & Seq
Forward (+)
49713 .. 51267
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 993 bp
atgcttctccagcccgattctggcacgattccgaccggaccgccttcatcttctctaggtcctcttctccagtcgctcacctgtagcccatctccgaccggtcccagccccgacgcagcccatcttcagtcgctcacctgcagtccatctccgccttcgtcttccccatgtcctcttctccagtcgctcgcctgcagcccatctccgaccggtctcagtttggtgaggttccggcaccgcagcgagatgggagcttcagatcgcgccgggatcgaggtcgacgaagaaggtgttgaaggcgtggtcgccactgccggctggagagagagagagagagagagagagagtctgagaggattgagatatgtgagttttagtttgattaatagggaggggtttccccgcgaggccattgacatcccaactgtgcgagctgaacgacatggtcagagtgatttagttgttaatgttggagattctacatcttcaaccaatgttccttcgcatgccatggatatggatctgagtgcaccatttgcattggttgatgagatagctgatgcatcaccagcaacggaggggggcatacaacttggagatcagattgtgaaatatggcaatgtggaaaatggtgacaatttgttacgggaacttgcttccgaggctcaaacaaatctgggttgtgctgtgcctgttatggtcttaaggcaaggtgcccaattcaacttaatggtgacacctagaacatggcaaggcaggggtctattggggtgtcatttccagattttacacagaagctatgttatccaggtaaggttgagcagcatgaatgggagtgtcatcaagagtaatgaaaccaagctcatggttaataccatctttggccaaggcgtctgcagtcatgttgcactccctgaaaatatgcctaatatgagcatccccatgttgttcatgagatttaggcaaccagtcagaagagaaccaagaggatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2

Protein Analysis

330

Amino Acids

35.58

Weight (kDa)

5.84

Isoelectric Point (pI)

57.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 146
AccB1I GGYRCC 2 cut(s) 234, 713
AccI GTMKAC 1 cut(s) 279
AccII CGCG 2 cut(s) 264, 405
AciI CCGC 4 cut(s) 41, 152, 238, 403
AclWI GGATC 2 cut(s) 278, 528
AcoI YGGCCR 1 cut(s) 883
AcuI CTGAAG 2 cut(s) 110, 240
AcyI GRCGYC 1 cut(s) 891
AfiI CCNNNNNNNGG 1 cut(s) 20
AflII CTTAAG 1 cut(s) 703
AgeI ACCGGT 2 cut(s) 98, 209
AgsI TTSAA 3 cut(s) 296, 489, 724
AjnI CCWGG 1 cut(s) 807
AluBI AGCT 5 cut(s) 254, 434, 557, 798, 862
AluI AGCT 5 cut(s) 254, 434, 557, 798, 862
Alw21I GWGCWC 1 cut(s) 532
Alw26I GTCTC 1 cut(s) 218
Alw44I GTGCAC 1 cut(s) 528
AlwI GGATC 2 cut(s) 278, 528
AoxI GGCC 2 cut(s) 408, 883
ApaLI GTGCAC 1 cut(s) 528
ApeKI GCWGC 4 cut(s) 116, 195, 240, 822
AseI ATTAAT 1 cut(s) 384
AsiGI ACCGGT 2 cut(s) 98, 209
AspLEI GCGC 1 cut(s) 266
AspS9I GGNCC 3 cut(s) 38, 59, 101
AsuC2I CCSGG 1 cut(s) 268
AsuHPI GGTGA 6 cut(s) 70, 127, 235, 558, 644, 745
AvaII GGWCC 3 cut(s) 38, 59, 101
BaeGI GKGCMC 2 cut(s) 532, 718
BalI TGGCCA 1 cut(s) 885
BanI GGYRCC 2 cut(s) 234, 713
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 532
BbvI GCAGC 4 cut(s) 128, 207, 252, 834
BccI CCATC 6 cut(s) 97, 129, 154, 208, 241, 884
BciT130I CCWGG 1 cut(s) 809
BcnI CCSGG 1 cut(s) 268
BcoDI GTCTC 1 cut(s) 218
BfaI CTAG 2 cut(s) 56, 741
BfmI CTRYAG 4 cut(s) 82, 139, 193, 895
BfrI CTTAAG 1 cut(s) 703
BfuAI ACCTGC 1 cut(s) 146
BisI GCNGC 4 cut(s) 117, 196, 241, 823
BlsI GCNGC 4 cut(s) 118, 197, 242, 824
Bme1390I CCNGG 2 cut(s) 268, 809
Bme18I GGWCC 3 cut(s) 38, 59, 101
BmgT120I GGNCC 3 cut(s) 38, 59, 101
BmiI GGNNCC 4 cut(s) 103, 230, 236, 715
BmrFI CCNGG 2 cut(s) 268, 809
BmsI GCATC 3 cut(s) 550, 572, 945
BpiI GAAGAC 1 cut(s) 153
BpmI CTGGAG 3 cut(s) 53, 164, 340
BpuMI CCSGG 1 cut(s) 268
BsaBI GATNNNNATC 1 cut(s) 519
BsaHI GRCGYC 1 cut(s) 891
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 3 cut(s) 510, 660, 886
BsaWI WCCGGW 3 cut(s) 35, 98, 209
Bsc4I CCNNNNNNNGG 1 cut(s) 20
Bse118I RCCGGY 3 cut(s) 98, 209, 314
Bse1I ACTGG 3 cut(s) 70, 181, 968
Bse3DI GCAATG 1 cut(s) 625
Bse8I GATNNNNATC 1 cut(s) 519
BseBI CCWGG 1 cut(s) 809
BseDI CCNNGG 3 cut(s) 510, 660, 886
BseGI GGATG 2 cut(s) 417, 936
BseJI GATNNNNATC 1 cut(s) 519
BseLI CCNNNNNNNGG 1 cut(s) 20
BseMI GCAATG 1 cut(s) 625
BseMII CTCAG 3 cut(s) 229, 341, 515
BseNI ACTGG 3 cut(s) 70, 181, 968
BseSI GKGCMC 2 cut(s) 532, 718
BseXI GCAGC 4 cut(s) 128, 207, 252, 834
BseYI CCCAGC 1 cut(s) 104
Bsh1236I CGCG 2 cut(s) 264, 405
Bsh1285I CGRYCG 3 cut(s) 36, 99, 210
BshFI GGCC 2 cut(s) 410, 885
BshNI GGYRCC 2 cut(s) 234, 713
BshTI ACCGGT 2 cut(s) 98, 209
BsiEI CGRYCG 3 cut(s) 36, 99, 210
BsiHKAI GWGCWC 1 cut(s) 532
BsiSI CCGG 6 cut(s) 36, 99, 210, 232, 267, 315
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 20
BsmAI GTCTC 1 cut(s) 218
BsmFI GGGAC 1 cut(s) 87
BsnI GGCC 2 cut(s) 410, 885
Bso31I GGTCTC 1 cut(s) 218
Bsp1286I GDGCHC 2 cut(s) 532, 718
Bsp143I GATC 4 cut(s) 259, 270, 520, 598
Bsp19I CCATGG 1 cut(s) 510
BspACI CCGC 4 cut(s) 41, 152, 238, 403
BspANI GGCC 2 cut(s) 410, 885
BspCNI CTCAG 3 cut(s) 228, 342, 516
BspFNI CGCG 2 cut(s) 264, 405
BspHI TCATGA 1 cut(s) 951
BspLI GGNNCC 4 cut(s) 103, 230, 236, 715
BspMAI CTGCAG 3 cut(s) 143, 197, 899
BspMI ACCTGC 1 cut(s) 146
BspPI GGATC 2 cut(s) 278, 528
BspT107I GGYRCC 2 cut(s) 234, 713
BspTI CTTAAG 1 cut(s) 703
BspTNI GGTCTC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 625
BsrFI RCCGGY 3 cut(s) 98, 209, 314
BsrI ACTGG 3 cut(s) 70, 181, 968
BssAI RCCGGY 3 cut(s) 98, 209, 314
BssECI CCNNGG 3 cut(s) 510, 660, 886
BssMI GATC 4 cut(s) 259, 270, 520, 598
BssNI GRCGYC 1 cut(s) 891
BssT1I CCWWGG 2 cut(s) 510, 886
Bst2UI CCWGG 1 cut(s) 809
Bst4CI ACNGT 1 cut(s) 427
Bst6I CTCTTC 3 cut(s) 69, 180, 970
BstACI GRCGYC 1 cut(s) 891
BstAFI CTTAAG 1 cut(s) 703
BstAPI GCANNNNNTGC 1 cut(s) 536
BstC8I GCNNGC 5 cut(s) 189, 193, 316, 432, 507
BstDEI CTNAG 3 cut(s) 215, 350, 524
BstDSI CCRYGG 1 cut(s) 510
BstF5I GGATG 2 cut(s) 417, 936
BstFNI CGCG 2 cut(s) 264, 405
BstHHI GCGC 1 cut(s) 266
BstKTI GATC 4 cut(s) 262, 273, 523, 601
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 4 cut(s) 259, 270, 520, 598
BstMCI CGRYCG 3 cut(s) 36, 99, 210
BstMWI GCNNNNNNNGC 4 cut(s) 10, 536, 569, 662
BstNI CCWGG 1 cut(s) 809
BstNSI RCATGY 1 cut(s) 509
BstSCI CCNGG 2 cut(s) 266, 807
BstSFI CTRYAG 4 cut(s) 82, 139, 193, 895
BstSLI GKGCMC 2 cut(s) 532, 718
BstUI CGCG 2 cut(s) 264, 405
BstV1I GCAGC 4 cut(s) 128, 207, 252, 834
BstV2I GAAGAC 1 cut(s) 153
BstX2I RGATCY 1 cut(s) 520
BstXI CCANNNNNNTGG 2 cut(s) 517, 865
BstYI RGATCY 1 cut(s) 520
BsuRI GGCC 2 cut(s) 410, 885
BtgI CCRYGG 1 cut(s) 510
BtsCI GGATG 2 cut(s) 417, 936
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 309
BveI ACCTGC 1 cut(s) 146
Cac8I GCNNGC 5 cut(s) 189, 193, 316, 432, 507
CciI TCATGA 1 cut(s) 951
CfoI GCGC 1 cut(s) 266
Cfr10I RCCGGY 3 cut(s) 98, 209, 314
Cfr13I GGNCC 3 cut(s) 38, 59, 101
CpoI CGGWCCG 1 cut(s) 38
CseI GACGC 2 cut(s) 122, 880
CspAI ACCGGT 2 cut(s) 98, 209
CspI CGGWCCG 1 cut(s) 38
DdeI CTNAG 3 cut(s) 215, 350, 524
DpnI GATC 4 cut(s) 261, 272, 522, 600
DpnII GATC 4 cut(s) 259, 270, 520, 598
EaeI YGGCCR 1 cut(s) 883
Eam1104I CTCTTC 3 cut(s) 69, 180, 970
EarI CTCTTC 3 cut(s) 69, 180, 970
EciI GGCGGA 1 cut(s) 141
Eco130I CCWWGG 2 cut(s) 510, 886
Eco31I GGTCTC 1 cut(s) 218
Eco47I GGWCC 3 cut(s) 38, 59, 101
Eco57I CTGAAG 2 cut(s) 110, 240
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 1 cut(s) 807
EcoT14I CCWWGG 2 cut(s) 510, 886
EcoT22I ATGCAT 1 cut(s) 565
ErhI CCWWGG 2 cut(s) 510, 886
FaqI GGGAC 1 cut(s) 87
FauI CCCGC 1 cut(s) 410
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 4 cut(s) 117, 196, 241, 823
FokI GGATG 2 cut(s) 404, 923
Fsp4HI GCNGC 4 cut(s) 117, 196, 241, 823
FspBI CTAG 2 cut(s) 56, 741
GlaI GCGC 1 cut(s) 265
GluI GCNGC 4 cut(s) 117, 196, 241, 823
GsaI CCCAGC 1 cut(s) 108
GsuI CTGGAG 3 cut(s) 53, 164, 340
HaeIII GGCC 2 cut(s) 410, 885
HapII CCGG 6 cut(s) 36, 99, 210, 232, 267, 315
HgaI GACGC 2 cut(s) 122, 880
HhaI GCGC 1 cut(s) 266
Hin1I GRCGYC 1 cut(s) 891
Hin6I GCGC 1 cut(s) 264
HinP1I GCGC 1 cut(s) 264
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 4 cut(s) 17, 28, 346, 476
HpaII CCGG 6 cut(s) 36, 99, 210, 232, 267, 315
HphI GGTGA 6 cut(s) 70, 127, 235, 558, 644, 745
Hpy166II GTNNAC 2 cut(s) 280, 530
Hpy188III TCNNGA 3 cut(s) 781, 844, 952
Hpy8I GTNNAC 2 cut(s) 280, 530
Hpy99I CGWCG 2 cut(s) 116, 284
HpyAV CCTTC 5 cut(s) 54, 165, 281, 290, 510
HpyCH4III ACNGT 1 cut(s) 427
HpyCH4V TGCA 7 cut(s) 141, 195, 530, 539, 563, 897, 908
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 536, 569, 662
HpyF3I CTNAG 3 cut(s) 215, 350, 524
Hsp92I GRCGYC 1 cut(s) 891
HspAI GCGC 1 cut(s) 264
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
Kzo9I GATC 4 cut(s) 259, 270, 520, 598
LmnI GCTCC 1 cut(s) 251
Lsp1109I GCAGC 4 cut(s) 128, 207, 252, 834
LweI GCATC 3 cut(s) 550, 572, 945
MaeI CTAG 2 cut(s) 56, 741
MaeIII GTNAC 3 cut(s) 632, 642, 733
MalI GATC 4 cut(s) 261, 272, 522, 600
MboI GATC 4 cut(s) 259, 270, 520, 598
MboII GAAGA 8 cut(s) 42, 56, 116, 153, 167, 296, 477, 987
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 2 cut(s) 532, 718
MlsI TGGCCA 1 cut(s) 885
MluCI AATT 2 cut(s) 637, 719
MluNI TGGCCA 1 cut(s) 885
MlyI GAGTC 1 cut(s) 355
MmeI TCCRAC 4 cut(s) 56, 119, 230, 451
Mox20I TGGCCA 1 cut(s) 885
Mph1103I ATGCAT 1 cut(s) 565
MroNI GCCGGC 1 cut(s) 314
MscI TGGCCA 1 cut(s) 885
MseI TTAA 5 cut(s) 384, 465, 704, 728, 870
MslI CAYNNNNRTG 2 cut(s) 515, 941
Msp20I TGGCCA 1 cut(s) 885
MspCI CTTAAG 1 cut(s) 703
MspI CCGG 6 cut(s) 36, 99, 210, 232, 267, 315
MspR9I CCNGG 2 cut(s) 268, 809
MvaI CCWGG 1 cut(s) 809
MvnI CGCG 2 cut(s) 264, 405
MwoI GCNNNNNNNGC 4 cut(s) 10, 536, 569, 662
NaeI GCCGGC 1 cut(s) 316
NciI CCSGG 1 cut(s) 268
NcoI CCATGG 1 cut(s) 510
NdeII GATC 4 cut(s) 259, 270, 520, 598
NgoMIV GCCGGC 1 cut(s) 314
NlaIV GGNNCC 4 cut(s) 103, 230, 236, 715
NmuCI GTSAC 2 cut(s) 632, 733
NsiI ATGCAT 1 cut(s) 565
NspI RCATGY 1 cut(s) 509
PaeI GCATGC 1 cut(s) 509
PagI TCATGA 1 cut(s) 951
PaqCI CACCTGC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PdiI GCCGGC 1 cut(s) 316
PfeI GAWTC 3 cut(s) 17, 28, 476
PflFI GACNNNGTC 1 cut(s) 444
PinAI ACCGGT 2 cut(s) 98, 209
PkrI GCNGC 4 cut(s) 118, 197, 242, 824
PleI GAGTC 1 cut(s) 354
PpsI GAGTC 1 cut(s) 354
PpuMI RGGWCCY 1 cut(s) 59
PshBI ATTAAT 1 cut(s) 384
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 807
PspFI CCCAGC 1 cut(s) 104
PspGI CCWGG 1 cut(s) 807
PspN4I GGNNCC 4 cut(s) 103, 230, 236, 715
PspPI GGNCC 3 cut(s) 38, 59, 101
PspPPI RGGWCCY 1 cut(s) 59
PstI CTGCAG 3 cut(s) 143, 197, 899
PsuI RGATCY 1 cut(s) 520
PsyI GACNNNGTC 1 cut(s) 444
RseI CAYNNNNRTG 2 cut(s) 515, 941
Rsr2I CGGWCCG 1 cut(s) 38
RsrII CGGWCCG 1 cut(s) 38
SalI GTCGAC 1 cut(s) 278
SaqAI TTAA 5 cut(s) 384, 465, 704, 728, 870
SatI GCNGC 4 cut(s) 117, 196, 241, 823
Sau3AI GATC 4 cut(s) 259, 270, 520, 598
Sau96I GGNCC 3 cut(s) 38, 59, 101
SchI GAGTC 1 cut(s) 355
ScrFI CCNGG 2 cut(s) 268, 809
SduI GDGCHC 2 cut(s) 532, 718
SfaNI GCATC 3 cut(s) 550, 572, 945
SfcI CTRYAG 4 cut(s) 82, 139, 193, 895
SinI GGWCC 3 cut(s) 38, 59, 101
SmiMI CAYNNNNRTG 2 cut(s) 515, 941
SmlI CTYRAG 1 cut(s) 703
SmoI CTYRAG 1 cut(s) 703
SphI GCATGC 1 cut(s) 509
Sse9I AATT 2 cut(s) 637, 719
SsiI CCGC 4 cut(s) 41, 152, 238, 403
SspMI CTAG 2 cut(s) 56, 741
StyD4I CCNGG 2 cut(s) 266, 807
StyI CCWWGG 2 cut(s) 510, 886
TaaI ACNGT 1 cut(s) 427
TaqI TCGA 2 cut(s) 273, 279
TasI AATT 2 cut(s) 637, 719
TfiI GAWTC 3 cut(s) 17, 28, 476
Tru1I TTAA 5 cut(s) 384, 465, 704, 728, 870
Tru9I TTAA 5 cut(s) 384, 465, 704, 728, 870
TscAI CASTG 1 cut(s) 316
TseFI GTSAC 2 cut(s) 632, 733
TseI GCWGC 4 cut(s) 116, 195, 240, 822
Tsp45I GTSAC 2 cut(s) 632, 733
TspDTI ATGAA 4 cut(s) 36, 842, 867, 940
TspGWI ACGGA 1 cut(s) 590
TspRI CASTG 1 cut(s) 316
Tth111I GACNNNGTC 1 cut(s) 444
Vha464I CTTAAG 1 cut(s) 703
VneI GTGCAC 1 cut(s) 528
VpaK11BI GGWCC 3 cut(s) 38, 59, 101
VspI ATTAAT 1 cut(s) 384
XceI RCATGY 1 cut(s) 509
XcmI CCANNNNNNNNNTGG 1 cut(s) 17
XmiI GTMKAC 1 cut(s) 279
XspI CTAG 2 cut(s) 56, 741
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.