Rmu_sc0006282.1_g000031

expansin-A13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006282.1
N/A
Physical Location & Seq
Reverse (-)
129561 .. 130109
549 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
atgtcgccgccaccgacgctaacgccggtgcttctcctcctcctcctattcacaccacccctcctctcccactcgtcttcttcttcttctgcgataaccttcaccgactccccctcaatctacgaatggcagtccgcacgcgccacctactacgcggcctcggaccctcgcgacgcggtgggcggcgcgtgcggctacggcgacctgacgaaggccggtttcggaatggcgacggtggggctgagcgaggcgctgttcgagcgtggccagatctgcggcgcgtgcttcgaggtccgctgcgtggaggacatgcggtggtgcattcccggaacctccatcattttgacggccacgaacttctgcgctcccaactatggcttcaccgccgagggtgggggccactgtaaccctccgaataagcacttggtcctcccgattgaggcctttgagaagatcgctatctggaaagctggtaatatgcccgtccagtatcgcaggtcggtttccttcgattcattccttaatcctcacttagagaatagagattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

182

Amino Acids

19.69

Weight (kDa)

5.51

Isoelectric Point (pI)

54.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 79 - 164 2.7e-17 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 486
AccII CGCG 6 cut(s) 141, 155, 171, 176, 188, 281
AcoI YGGCCR 2 cut(s) 265, 348
AfiI CCNNNNNNNGG 5 cut(s) 211, 301, 374, 393, 439
AjuI GAANNNNNNNTTGG 4 cut(s) 362, 394, 407, 439
AluBI AGCT 1 cut(s) 470
AluI AGCT 1 cut(s) 470
AoxI GGCC 6 cut(s) 156, 213, 265, 348, 397, 441
ApeKI GCWGC 1 cut(s) 297
AspLEI GCGC 5 cut(s) 143, 188, 253, 281, 365
AspS9I GGNCC 4 cut(s) 163, 292, 397, 427
AsuC2I CCSGG 1 cut(s) 327
AsuHPI GGTGA 2 cut(s) 94, 373
AvaII GGWCC 3 cut(s) 163, 292, 427
BalI TGGCCA 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 69
BbvI GCAGC 1 cut(s) 284
BccI CCATC 1 cut(s) 344
BceAI ACGGC 2 cut(s) 214, 363
BcnI CCSGG 1 cut(s) 327
BfoI RGCGCY 1 cut(s) 254
BfuAI ACCTGC 1 cut(s) 486
BglII AGATCT 1 cut(s) 270
BisI GCNGC 6 cut(s) 8, 156, 184, 193, 277, 298
BlpI GCTNAGC 1 cut(s) 242
BlsI GCNGC 6 cut(s) 9, 157, 185, 194, 278, 299
Bme1390I CCNGG 1 cut(s) 327
Bme18I GGWCC 3 cut(s) 163, 292, 427
BmgT120I GGNCC 4 cut(s) 163, 292, 397, 427
BmiI GGNNCC 3 cut(s) 165, 331, 398
BmrFI CCNGG 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 69
Bpu1102I GCTNAGC 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 327
BsaBI GATNNNNATC 1 cut(s) 458
BsaJI CCNNGG 2 cut(s) 159, 387
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 5 cut(s) 211, 301, 374, 393, 439
Bse118I RCCGGY 2 cut(s) 25, 215
Bse1I ACTGG 1 cut(s) 487
Bse8I GATNNNNATC 1 cut(s) 458
BseDI CCNNGG 2 cut(s) 159, 387
BseJI GATNNNNATC 1 cut(s) 458
BseLI CCNNNNNNNGG 5 cut(s) 211, 301, 374, 393, 439
BseMII CTCAG 1 cut(s) 233
BseNI ACTGG 1 cut(s) 487
BseRI GAGGAG 4 cut(s) 26, 29, 32, 53
BseXI GCAGC 1 cut(s) 284
Bsh1236I CGCG 6 cut(s) 141, 155, 171, 176, 188, 281
BshFI GGCC 6 cut(s) 158, 215, 267, 350, 399, 443
BsiSI CCGG 3 cut(s) 26, 216, 327
BslI CCNNNNNNNGG 5 cut(s) 211, 301, 374, 393, 439
BsmI GAATGC 1 cut(s) 321
BsnI GGCC 6 cut(s) 158, 215, 267, 350, 399, 443
Bsp143I GATC 2 cut(s) 270, 453
Bsp1720I GCTNAGC 1 cut(s) 242
Bsp68I TCGCGA 1 cut(s) 171
BspANI GGCC 6 cut(s) 158, 215, 267, 350, 399, 443
BspCNI CTCAG 1 cut(s) 234
BspFNI CGCG 6 cut(s) 141, 155, 171, 176, 188, 281
BspLI GGNNCC 3 cut(s) 165, 331, 398
BspMI ACCTGC 1 cut(s) 486
BsrFI RCCGGY 2 cut(s) 25, 215
BsrI ACTGG 1 cut(s) 487
BssAI RCCGGY 2 cut(s) 25, 215
BssECI CCNNGG 2 cut(s) 159, 387
BssMI GATC 2 cut(s) 270, 453
Bst4CI ACNGT 2 cut(s) 235, 404
BstC8I GCNNGC 3 cut(s) 139, 190, 283
BstDEI CTNAG 2 cut(s) 242, 532
BstENI CCTNNNNNAGG 1 cut(s) 209
BstFNI CGCG 6 cut(s) 141, 155, 171, 176, 188, 281
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 5 cut(s) 143, 188, 253, 281, 365
BstKTI GATC 2 cut(s) 273, 456
BstMBI GATC 2 cut(s) 270, 453
BstMWI GCNNNNNNNGC 7 cut(s) 16, 189, 192, 198, 259, 273, 282
BstNSI RCATGY 1 cut(s) 313
BstSCI CCNGG 1 cut(s) 325
BstUI CGCG 6 cut(s) 141, 155, 171, 176, 188, 281
BstV1I GCAGC 1 cut(s) 284
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
BsuRI GGCC 6 cut(s) 158, 215, 267, 350, 399, 443
BtsIMutI CAGTG 1 cut(s) 400
BtuMI TCGCGA 1 cut(s) 171
BveI ACCTGC 1 cut(s) 486
Cac8I GCNNGC 3 cut(s) 139, 190, 283
CfoI GCGC 5 cut(s) 143, 188, 253, 281, 365
Cfr10I RCCGGY 2 cut(s) 25, 215
Cfr13I GGNCC 4 cut(s) 163, 292, 397, 427
CseI GACGC 2 cut(s) 25, 182
CviAII CATG 1 cut(s) 310
DdeI CTNAG 2 cut(s) 242, 532
DpnI GATC 2 cut(s) 272, 455
DpnII GATC 2 cut(s) 270, 453
EaeI YGGCCR 2 cut(s) 265, 348
Eco147I AGGCCT 1 cut(s) 443
Eco47I GGWCC 3 cut(s) 163, 292, 427
EcoNI CCTNNNNNAGG 1 cut(s) 209
FaeI CATG 1 cut(s) 313
FaiI YATR 3 cut(s) 311, 375, 479
FatI CATG 1 cut(s) 309
Fnu4HI GCNGC 6 cut(s) 8, 156, 184, 193, 277, 298
Fsp4HI GCNGC 6 cut(s) 8, 156, 184, 193, 277, 298
GlaI GCGC 5 cut(s) 142, 187, 252, 280, 364
GluI GCNGC 6 cut(s) 8, 156, 184, 193, 277, 298
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 6 cut(s) 158, 215, 267, 350, 399, 443
HapII CCGG 3 cut(s) 26, 216, 327
HgaI GACGC 2 cut(s) 25, 182
HhaI GCGC 5 cut(s) 143, 188, 253, 281, 365
Hin1II CATG 1 cut(s) 313
Hin6I GCGC 5 cut(s) 141, 186, 251, 279, 363
HinP1I GCGC 5 cut(s) 141, 186, 251, 279, 363
HinfI GANTC 2 cut(s) 107, 512
HpaII CCGG 3 cut(s) 26, 216, 327
HphI GGTGA 2 cut(s) 94, 373
Hpy188I TCNGA 3 cut(s) 163, 224, 414
Hpy188III TCNNGA 3 cut(s) 170, 433, 463
Hpy99I CGWCG 3 cut(s) 19, 176, 235
HpyAV CCTTC 3 cut(s) 109, 205, 517
HpyCH4III ACNGT 2 cut(s) 235, 404
HpyCH4V TGCA 1 cut(s) 321
HpyF10VI GCNNNNNNNGC 7 cut(s) 16, 189, 192, 198, 259, 273, 282
HpyF3I CTNAG 2 cut(s) 242, 532
Hsp92II CATG 1 cut(s) 313
HspAI GCGC 5 cut(s) 141, 186, 251, 279, 363
Kzo9I GATC 2 cut(s) 270, 453
LmnI GCTCC 1 cut(s) 370
LpnPI CCDG 9 cut(s) 39, 218, 229, 281, 340, 448, 456, 481, 500
Lsp1109I GCAGC 1 cut(s) 284
MaeIII GTNAC 1 cut(s) 404
MalI GATC 2 cut(s) 272, 455
MboI GATC 2 cut(s) 270, 453
MboII GAAGA 5 cut(s) 69, 72, 75, 78, 463
MflI RGATCY 1 cut(s) 270
MlsI TGGCCA 1 cut(s) 267
MluNI TGGCCA 1 cut(s) 267
MlyI GAGTC 1 cut(s) 101
Mox20I TGGCCA 1 cut(s) 267
MscI TGGCCA 1 cut(s) 267
MseI TTAA 1 cut(s) 522
Msp20I TGGCCA 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 297
MspI CCGG 3 cut(s) 26, 216, 327
MspR9I CCNGG 1 cut(s) 327
Mva1269I GAATGC 1 cut(s) 321
MvnI CGCG 6 cut(s) 141, 155, 171, 176, 188, 281
MwoI GCNNNNNNNGC 7 cut(s) 16, 189, 192, 198, 259, 273, 282
NciI CCSGG 1 cut(s) 327
NdeII GATC 2 cut(s) 270, 453
NlaIII CATG 1 cut(s) 313
NlaIV GGNNCC 3 cut(s) 165, 331, 398
NmeAIII GCCGAG 1 cut(s) 412
NruI TCGCGA 1 cut(s) 171
NspI RCATGY 1 cut(s) 313
PceI AGGCCT 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 1 cut(s) 321
PfeI GAWTC 1 cut(s) 512
PfoI TCCNGGA 1 cut(s) 325
PkrI GCNGC 6 cut(s) 9, 157, 185, 194, 278, 299
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PspN4I GGNNCC 3 cut(s) 165, 331, 398
PspPI GGNCC 4 cut(s) 163, 292, 397, 427
PsuI RGATCY 1 cut(s) 270
RruI TCGCGA 1 cut(s) 171
SaqAI TTAA 1 cut(s) 522
SatI GCNGC 6 cut(s) 8, 156, 184, 193, 277, 298
Sau3AI GATC 2 cut(s) 270, 453
Sau96I GGNCC 4 cut(s) 163, 292, 397, 427
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 327
SetI ASST 7 cut(s) 101, 149, 207, 294, 335, 472, 500
SgrAI CRCCGGYG 1 cut(s) 25
SinI GGWCC 3 cut(s) 163, 292, 427
SseBI AGGCCT 1 cut(s) 443
StuI AGGCCT 1 cut(s) 443
StyD4I CCNGG 1 cut(s) 325
TaaI ACNGT 2 cut(s) 235, 404
TaqI TCGA 3 cut(s) 258, 288, 510
TauI GCSGC 5 cut(s) 10, 158, 186, 195, 279
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TscAI CASTG 1 cut(s) 407
TseI GCWGC 1 cut(s) 297
TspDTI ATGAA 1 cut(s) 504
TspRI CASTG 1 cut(s) 407
VpaK11BI GGWCC 3 cut(s) 163, 292, 427
XagI CCTNNNNNAGG 1 cut(s) 209
XceI RCATGY 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.