Rmu_sc0006590.1_g000003

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006590.1
N/A
Physical Location & Seq
Forward (+)
7117 .. 8224
1108 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 879 bp
atggcatcagttgtttacatcaccgatggtcaaactgggaggcctattgatgattctgagcgtctttctgaaattaaagagtttctccttaatgtgctcaaggcagacaaagatatgcgctctgccaacactgccgtttctgtaggttctacacacaaggagaggaggcttcaccagatgatgtatgcagatcgtgattatgatctacacagttcagattctggatcagccagtgatagaagcaaaccctttgtaactgtcgaaaattgtgcagataagggatatactgttgtgaacttgaggtgtccagatcgtcctaagttgctctttgatacagtatgcaccttaacagatatgcaatatgtggtatatcatgcaactgtgattgctgaaggaccagaggcttatcaggaatattatataagacatactgatggctgccctataagttctgaaggagagaggcagagggtaatccattgcttggaggctgctattcgaaggcgaacttctgagggtataagactagaactctgtggtgatgacagagccgggcttctttctgacgtgactcgcatattcagagaaaacagcctttcagtcacccgagctgaggttatgaccaggggttcccaagctgtgaacgtcttctacgtgactggtgcatctggaactccagtgacgagtgagacaattgaggcagttcggcaagagattggcttgacaatactgcgcgtagaagaagacatgtactcaaaatctccaccacaggagagtgggagattctctttgggaaatctattccggacgagatcagagaaattcctttacaacttgggtttgataaagtcgtgttctttagcgtatccaattaggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

292

Amino Acids

32.78

Weight (kDa)

5.94

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 176 - 236 6.4e-08 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 484
AccII CGCG 1 cut(s) 735
AccIII TCCGGA 1 cut(s) 804
AclWI GGATC 1 cut(s) 232
AcsI RAATTY 1 cut(s) 821
AcuI CTGAAG 2 cut(s) 411, 474
AfaI GTAC 1 cut(s) 752
AfiI CCNNNNNNNGG 2 cut(s) 484, 613
AflIII ACRYGT 1 cut(s) 747
AjiI CACGTC 1 cut(s) 568
AjnI CCWGG 1 cut(s) 623
AluBI AGCT 2 cut(s) 611, 638
AluI AGCT 2 cut(s) 611, 638
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 1 cut(s) 683
AlwI GGATC 1 cut(s) 232
AlwNI CAGNNNCTG 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 606
Aor13HI TCCGGA 1 cut(s) 804
AoxI GGCC 1 cut(s) 41
ApeKI GCWGC 2 cut(s) 438, 491
ApoI RAATTY 1 cut(s) 821
Asp700I GAANNNNTTC 1 cut(s) 647
AspLEI GCGC 2 cut(s) 120, 735
AspS9I GGNCC 1 cut(s) 395
AsuC2I CCSGG 1 cut(s) 553
AsuHPI GGTGA 4 cut(s) 13, 164, 551, 595
AsuII TTCGAA 1 cut(s) 499
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 1 cut(s) 395
BbsI GAAGAC 2 cut(s) 640, 750
Bbv12I GWGCWC 1 cut(s) 99
BbvCI CCTCAGC 1 cut(s) 612
BbvI GCAGC 2 cut(s) 425, 478
BccI CCATC 2 cut(s) 20, 428
BceAI ACGGC 1 cut(s) 119
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 1 cut(s) 625
BciVI GTATCC 1 cut(s) 876
BcnI CCSGG 1 cut(s) 553
BcoDI GTCTC 1 cut(s) 683
BfaI CTAG 1 cut(s) 527
BfmI CTRYAG 1 cut(s) 141
BfuI GTATCC 1 cut(s) 876
BisI GCNGC 2 cut(s) 439, 492
BlsI GCNGC 2 cut(s) 440, 493
Bme1390I CCNGG 2 cut(s) 553, 625
Bme18I GGWCC 1 cut(s) 395
BmeT110I CYCGRG 1 cut(s) 606
BmgBI CACGTC 1 cut(s) 568
BmgT120I GGNCC 1 cut(s) 395
BmiI GGNNCC 1 cut(s) 631
BmrFI CCNGG 2 cut(s) 553, 625
BmrI ACTGGG 1 cut(s) 45
BmsI GCATC 2 cut(s) 14, 674
BmuI ACTGGG 1 cut(s) 45
BpiI GAAGAC 2 cut(s) 640, 750
BpmI CTGGAG 1 cut(s) 660
Bpu10I CCTNAGC 1 cut(s) 612
Bpu14I TTCGAA 1 cut(s) 499
BpuEI CTTGAG 2 cut(s) 83, 319
BpuMI CCSGG 1 cut(s) 553
BsaAI YACGTR 1 cut(s) 655
BsaBI GATNNNNATC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 624
BsaWI WCCGGW 1 cut(s) 804
Bsc4I CCNNNNNNNGG 2 cut(s) 484, 613
Bse1I ACTGG 4 cut(s) 40, 231, 664, 677
Bse3DI GCAATG 1 cut(s) 478
Bse8I GATNNNNATC 1 cut(s) 201
BseAI TCCGGA 1 cut(s) 804
BseBI CCWGG 1 cut(s) 625
BseDI CCNNGG 1 cut(s) 624
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 2 cut(s) 484, 613
BseMI GCAATG 1 cut(s) 478
BseMII CTCAG 3 cut(s) 48, 504, 603
BseNI ACTGG 4 cut(s) 40, 231, 664, 677
BseRI GAGGAG 1 cut(s) 178
BseXI GCAGC 2 cut(s) 425, 478
BsgI GTGCAG 1 cut(s) 291
Bsh1236I CGCG 1 cut(s) 735
BshFI GGCC 1 cut(s) 43
BsiHKAI GWGCWC 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 2 cut(s) 552, 805
BslI CCNNNNNNNGG 2 cut(s) 484, 613
BsmAI GTCTC 1 cut(s) 683
BsnI GGCC 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 606
Bsp119I TTCGAA 1 cut(s) 499
Bsp1286I GDGCHC 1 cut(s) 99
Bsp13I TCCGGA 1 cut(s) 804
Bsp143I GATC 5 cut(s) 190, 202, 224, 310, 812
BspANI GGCC 1 cut(s) 43
BspCNI CTCAG 3 cut(s) 49, 505, 604
BspEI TCCGGA 1 cut(s) 804
BspFNI CGCG 1 cut(s) 735
BspLI GGNNCC 1 cut(s) 631
BspPI GGATC 1 cut(s) 232
BspT104I TTCGAA 1 cut(s) 499
BsrDI GCAATG 1 cut(s) 478
BsrI ACTGG 4 cut(s) 40, 231, 664, 677
BssECI CCNNGG 1 cut(s) 624
BssMI GATC 5 cut(s) 190, 202, 224, 310, 812
Bst2UI CCWGG 1 cut(s) 625
Bst4CI ACNGT 5 cut(s) 212, 259, 289, 337, 382
BstBAI YACGTR 1 cut(s) 655
BstBI TTCGAA 1 cut(s) 499
BstDEI CTNAG 4 cut(s) 57, 318, 513, 612
BstFNI CGCG 1 cut(s) 735
BstHHI GCGC 2 cut(s) 120, 735
BstKTI GATC 5 cut(s) 193, 205, 227, 313, 815
BstMAI GTCTC 1 cut(s) 683
BstMBI GATC 5 cut(s) 190, 202, 224, 310, 812
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNI CCWGG 1 cut(s) 625
BstNSI RCATGY 1 cut(s) 751
BstSCI CCNGG 2 cut(s) 551, 623
BstSFI CTRYAG 1 cut(s) 141
BstUI CGCG 1 cut(s) 735
BstV1I GCAGC 2 cut(s) 425, 478
BstV2I GAAGAC 2 cut(s) 640, 750
BsuI GTATCC 1 cut(s) 876
BsuRI GGCC 1 cut(s) 43
BtrI CACGTC 1 cut(s) 568
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 3 cut(s) 129, 238, 684
CaiI CAGNNNCTG 1 cut(s) 221
CfoI GCGC 2 cut(s) 120, 735
Cfr13I GGNCC 1 cut(s) 395
CseI GACGC 1 cut(s) 50
Csp6I GTAC 1 cut(s) 751
CviAII CATG 2 cut(s) 374, 748
CviQI GTAC 1 cut(s) 751
DdeI CTNAG 4 cut(s) 57, 318, 513, 612
DpnI GATC 5 cut(s) 192, 204, 226, 312, 814
DpnII GATC 5 cut(s) 190, 202, 224, 310, 812
Eco147I AGGCCT 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 395
Eco57I CTGAAG 2 cut(s) 411, 474
Eco88I CYCGRG 1 cut(s) 606
EcoRII CCWGG 1 cut(s) 623
FaeI CATG 2 cut(s) 377, 751
FalI AAGNNNNNCTT 4 cut(s) 311, 343, 493, 525
FatI CATG 2 cut(s) 373, 747
Fnu4HI GCNGC 2 cut(s) 439, 492
Fsp4HI GCNGC 2 cut(s) 439, 492
FspBI CTAG 1 cut(s) 527
GlaI GCGC 2 cut(s) 119, 734
GluI GCNGC 2 cut(s) 439, 492
GsuI CTGGAG 1 cut(s) 660
HaeIII GGCC 1 cut(s) 43
HapII CCGG 2 cut(s) 552, 805
HgaI GACGC 1 cut(s) 50
HhaI GCGC 2 cut(s) 120, 735
Hin1II CATG 2 cut(s) 377, 751
Hin6I GCGC 2 cut(s) 118, 733
HinP1I GCGC 2 cut(s) 118, 733
HinfI GANTC 4 cut(s) 53, 218, 571, 783
HpaII CCGG 2 cut(s) 552, 805
HphI GGTGA 4 cut(s) 13, 164, 551, 595
Hpy166II GTNNAC 3 cut(s) 16, 295, 643
Hpy188I TCNGA 8 cut(s) 58, 70, 217, 454, 514, 565, 584, 817
Hpy188III TCNNGA 6 cut(s) 194, 222, 308, 410, 669, 805
Hpy8I GTNNAC 3 cut(s) 16, 295, 643
HpyAV CCTTC 3 cut(s) 386, 449, 495
HpyCH4III ACNGT 5 cut(s) 212, 259, 289, 337, 382
HpyCH4IV ACGT 3 cut(s) 567, 645, 654
HpyCH4V TGCA 6 cut(s) 188, 272, 342, 358, 377, 665
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 4 cut(s) 57, 318, 513, 612
HpySE526I ACGT 3 cut(s) 567, 645, 654
Hsp92II CATG 2 cut(s) 377, 751
HspAI GCGC 2 cut(s) 118, 733
Kpn2I TCCGGA 1 cut(s) 804
Kzo9I GATC 5 cut(s) 190, 202, 224, 310, 812
Lsp1109I GCAGC 2 cut(s) 425, 478
LweI GCATC 2 cut(s) 14, 674
MaeI CTAG 1 cut(s) 527
MaeII ACGT 3 cut(s) 567, 645, 654
MaeIII GTNAC 5 cut(s) 253, 568, 601, 655, 679
MalI GATC 5 cut(s) 192, 204, 226, 312, 814
MboI GATC 5 cut(s) 190, 202, 224, 310, 812
MboII GAAGA 3 cut(s) 640, 752, 755
MfeI CAATTG 1 cut(s) 693
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 5 cut(s) 72, 265, 693, 821, 870
MlyI GAGTC 1 cut(s) 565
MroI TCCGGA 1 cut(s) 804
MroXI GAANNNNTTC 1 cut(s) 647
MseI TTAA 3 cut(s) 75, 90, 347
MslI CAYNNNNRTG 1 cut(s) 432
MspI CCGG 2 cut(s) 552, 805
MspR9I CCNGG 2 cut(s) 553, 625
MunI CAATTG 1 cut(s) 693
MvaI CCWGG 1 cut(s) 625
MvnI CGCG 1 cut(s) 735
MwoI GCNNNNNNNGC 1 cut(s) 131
NciI CCSGG 1 cut(s) 553
NdeII GATC 5 cut(s) 190, 202, 224, 310, 812
NlaIII CATG 2 cut(s) 377, 751
NlaIV GGNNCC 1 cut(s) 631
NmuCI GTSAC 4 cut(s) 568, 601, 655, 679
NspI RCATGY 1 cut(s) 751
NspV TTCGAA 1 cut(s) 499
PceI AGGCCT 1 cut(s) 43
PciI ACATGT 1 cut(s) 747
PcsI WCGNNNNNNNCGW 1 cut(s) 651
PdmI GAANNNNTTC 1 cut(s) 647
PfeI GAWTC 3 cut(s) 53, 218, 783
PflMI CCANNNNNTGG 1 cut(s) 484
PkrI GCNGC 2 cut(s) 440, 493
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Ppu21I YACGTR 1 cut(s) 655
PscI ACATGT 1 cut(s) 747
Psp6I CCWGG 1 cut(s) 623
PspGI CCWGG 1 cut(s) 623
PspN4I GGNNCC 1 cut(s) 631
PspPI GGNCC 1 cut(s) 395
PsrI GAACNNNNNNTAC 2 cut(s) 635, 667
PstNI CAGNNNCTG 1 cut(s) 221
RsaI GTAC 1 cut(s) 752
RsaNI GTAC 1 cut(s) 751
RseI CAYNNNNRTG 1 cut(s) 432
SaqAI TTAA 3 cut(s) 75, 90, 347
SatI GCNGC 2 cut(s) 439, 492
Sau3AI GATC 5 cut(s) 190, 202, 224, 310, 812
Sau96I GGNCC 1 cut(s) 395
SchI GAGTC 1 cut(s) 565
ScrFI CCNGG 2 cut(s) 553, 625
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 2 cut(s) 14, 674
SfcI CTRYAG 1 cut(s) 141
SfuI TTCGAA 1 cut(s) 499
SinI GGWCC 1 cut(s) 395
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 2 cut(s) 98, 298
SmoI CTYRAG 2 cut(s) 98, 298
Sse9I AATT 5 cut(s) 72, 265, 693, 821, 870
SseBI AGGCCT 1 cut(s) 43
SspI AATATT 1 cut(s) 416
SspMI CTAG 1 cut(s) 527
StuI AGGCCT 1 cut(s) 43
StyD4I CCNGG 2 cut(s) 551, 623
TaaI ACNGT 5 cut(s) 212, 259, 289, 337, 382
TaiI ACGT 3 cut(s) 570, 648, 657
TaqI TCGA 2 cut(s) 261, 499
TasI AATT 5 cut(s) 72, 265, 693, 821, 870
TatI WGTACW 1 cut(s) 750
TfiI GAWTC 3 cut(s) 53, 218, 783
Tru1I TTAA 3 cut(s) 75, 90, 347
Tru9I TTAA 3 cut(s) 75, 90, 347
TscAI CASTG 3 cut(s) 136, 238, 684
TseFI GTSAC 4 cut(s) 568, 601, 655, 679
TseI GCWGC 2 cut(s) 438, 491
Tsp45I GTSAC 4 cut(s) 568, 601, 655, 679
TspRI CASTG 3 cut(s) 136, 238, 684
Van91I CCANNNNNTGG 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 395
XapI RAATTY 1 cut(s) 821
XceI RCATGY 1 cut(s) 751
XmnI GAANNNNTTC 1 cut(s) 647
XspI CTAG 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.