Rmu_sc0006638.1_g000012

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006638.1
N/A
Physical Location & Seq
Forward (+)
27274 .. 28581
1308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006638.1_g000012.1.cds

Sequence Viewer

Length: 663 bp
atgaaaaatggagctccagtttgtgggagtgagatggtggcagcttcagttcattctagccggtctgaaggtgttcaggagagctcgtcgagtgagctgagtcattcgacagttgaaccaagctcaaggaggaagagagaagcccggagcagccgcagtccatttctatgtgatagattgatcactgaatggaagagtgcaatcgataggctcaggaataaaccagaaactagcatcattgatgtgcttcagataagttttgatgggctaagggaaacagaaaaggaaattttcctacatgtcgcttgcttttatgtggggaagaatagggattgtggatctgaaatactagattattgtcagctttaccctgacattggtttaagtgttcttgctgataaatctctcattactatttccaataacaaactatcgatgcatgctttgctacaagaaatcggctgggagatagttcgtaggaagtcacctaaaccaggcaagcgtagtagattatggtctcatgaagacatccacaatggaacagaagcagttcaaggcatggtaatggacttgcctaaattagaagtggctaattggaaccccaaagccttctcaaagttgtctgacactactagcaaaacaacaattacccacggattttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

220

Amino Acids

24.63

Weight (kDa)

8.26

Isoelectric Point (pI)

68.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 23
AciI CCGC 1 cut(s) 154
AclWI GGATC 1 cut(s) 346
AcsI RAATTY 1 cut(s) 288
AcuI CTGAAG 3 cut(s) 30, 87, 233
AfiI CCNNNNNNNGG 3 cut(s) 23, 377, 494
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 2 cut(s) 116, 554
AjnI CCWGG 1 cut(s) 493
AluBI AGCT 6 cut(s) 14, 44, 84, 97, 123, 364
AluI AGCT 6 cut(s) 14, 44, 84, 97, 123, 364
Alw21I GWGCWC 2 cut(s) 16, 86
Alw26I GTCTC 1 cut(s) 522
AlwI GGATC 1 cut(s) 346
ApeKI GCWGC 2 cut(s) 41, 150
ApoI RAATTY 1 cut(s) 288
Asp700I GAANNNNTTC 2 cut(s) 72, 549
AsuC2I CCSGG 1 cut(s) 145
AsuHPI GGTGA 1 cut(s) 477
BanII GRGCYC 2 cut(s) 16, 86
BbsI GAAGAC 1 cut(s) 531
Bbv12I GWGCWC 2 cut(s) 16, 86
BbvI GCAGC 2 cut(s) 53, 162
BccI CCATC 2 cut(s) 28, 257
BciT130I CCWGG 1 cut(s) 495
BclI TGATCA 1 cut(s) 180
BcnI CCSGG 1 cut(s) 145
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 4 cut(s) 57, 231, 350, 633
BisI GCNGC 3 cut(s) 42, 151, 154
BlsI GCNGC 3 cut(s) 43, 152, 155
Bme1390I CCNGG 2 cut(s) 145, 495
BmiI GGNNCC 1 cut(s) 599
BmrFI CCNGG 2 cut(s) 145, 495
BmsI GCATC 2 cut(s) 243, 426
BpiI GAAGAC 1 cut(s) 531
Bpu10I CCTNAGC 2 cut(s) 212, 269
BpuEI CTTGAG 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 145
Bsa29I ATCGAT 2 cut(s) 204, 434
BsaI GGTCTC 1 cut(s) 522
BsaJI CCNNGG 1 cut(s) 652
BsaXI ACNNNNNCTCC 3 cut(s) 33, 71, 101
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 377, 494
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 1 cut(s) 17
BseBI CCWGG 1 cut(s) 495
BseCI ATCGAT 2 cut(s) 204, 434
BseDI CCNNGG 1 cut(s) 652
BseGI GGATG 1 cut(s) 528
BseLI CCNNNNNNNGG 3 cut(s) 23, 377, 494
BseMII CTCAG 2 cut(s) 89, 226
BseNI ACTGG 1 cut(s) 17
BseXI GCAGC 2 cut(s) 53, 162
BseYI CCCAGC 1 cut(s) 462
BshVI ATCGAT 2 cut(s) 204, 434
BsiHKAI GWGCWC 2 cut(s) 16, 86
BsiSI CCGG 2 cut(s) 61, 145
BslI CCNNNNNNNGG 3 cut(s) 23, 377, 494
BsmAI GTCTC 1 cut(s) 522
Bso31I GGTCTC 1 cut(s) 522
Bsp1286I GDGCHC 2 cut(s) 16, 86
Bsp143I GATC 2 cut(s) 180, 338
BspACI CCGC 1 cut(s) 154
BspCNI CTCAG 2 cut(s) 90, 225
BspDI ATCGAT 2 cut(s) 204, 434
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 1 cut(s) 599
BspPI GGATC 1 cut(s) 346
BspTNI GGTCTC 1 cut(s) 522
BsrFI RCCGGY 1 cut(s) 60
BsrI ACTGG 1 cut(s) 17
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 652
BssMI GATC 2 cut(s) 180, 338
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 1 cut(s) 112
Bst6I CTCTTC 2 cut(s) 128, 188
BstAPI GCANNNNNTGC 1 cut(s) 445
BstC8I GCNNGC 3 cut(s) 307, 441, 500
BstDEI CTNAG 3 cut(s) 98, 212, 269
BstDSI CCRYGG 1 cut(s) 652
BstENI CCTNNNNNAGG 1 cut(s) 492
BstF5I GGATG 1 cut(s) 528
BstKTI GATC 2 cut(s) 183, 341
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 2 cut(s) 180, 338
BstMWI GCNNNNNNNGC 1 cut(s) 445
BstNI CCWGG 1 cut(s) 495
BstNSI RCATGY 2 cut(s) 302, 443
BstSCI CCNGG 2 cut(s) 143, 493
BstV1I GCAGC 2 cut(s) 53, 162
BstV2I GAAGAC 1 cut(s) 531
BstX2I RGATCY 1 cut(s) 338
BstYI RGATCY 1 cut(s) 338
Bsu15I ATCGAT 2 cut(s) 204, 434
BsuTUI ATCGAT 2 cut(s) 204, 434
BtgI CCRYGG 1 cut(s) 652
BtsCI GGATG 1 cut(s) 528
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 3 cut(s) 307, 441, 500
CciI TCATGA 1 cut(s) 520
Cfr10I RCCGGY 1 cut(s) 60
ClaI ATCGAT 2 cut(s) 204, 434
CviAII CATG 4 cut(s) 299, 440, 521, 559
DdeI CTNAG 3 cut(s) 98, 212, 269
DpnI GATC 2 cut(s) 182, 340
DpnII GATC 2 cut(s) 180, 338
Eam1104I CTCTTC 2 cut(s) 128, 188
EarI CTCTTC 2 cut(s) 128, 188
Ecl136II GAGCTC 2 cut(s) 14, 84
Eco24I GRGCYC 2 cut(s) 16, 86
Eco31I GGTCTC 1 cut(s) 522
Eco53kI GAGCTC 2 cut(s) 14, 84
Eco57I CTGAAG 3 cut(s) 30, 87, 233
EcoICRI GAGCTC 2 cut(s) 14, 84
EcoNI CCTNNNNNAGG 1 cut(s) 492
EcoRII CCWGG 1 cut(s) 493
EcoT22I ATGCAT 1 cut(s) 441
EcoT38I GRGCYC 2 cut(s) 16, 86
FaeI CATG 4 cut(s) 302, 443, 524, 562
FaiI YATR 7 cut(s) 169, 300, 315, 441, 514, 522, 560
FatI CATG 4 cut(s) 298, 439, 520, 558
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 3 cut(s) 42, 151, 154
FokI GGATG 1 cut(s) 515
FriOI GRGCYC 2 cut(s) 16, 86
Fsp4HI GCNGC 3 cut(s) 42, 151, 154
FspBI CTAG 4 cut(s) 57, 231, 350, 633
GluI GCNGC 3 cut(s) 42, 151, 154
GsaI CCCAGC 1 cut(s) 466
HapII CCGG 2 cut(s) 61, 145
Hin1II CATG 4 cut(s) 302, 443, 524, 562
HinfI GANTC 1 cut(s) 100
HpaII CCGG 2 cut(s) 61, 145
HphI GGTGA 1 cut(s) 477
Hpy188I TCNGA 4 cut(s) 67, 252, 343, 625
Hpy188III TCNNGA 3 cut(s) 77, 214, 521
Hpy99I CGWCG 1 cut(s) 91
HpyAV CCTTC 2 cut(s) 62, 619
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4V TGCA 2 cut(s) 200, 439
HpyF10VI GCNNNNNNNGC 1 cut(s) 445
HpyF3I CTNAG 3 cut(s) 98, 212, 269
Hsp92II CATG 4 cut(s) 302, 443, 524, 562
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 2 cut(s) 180, 338
LmnI GCTCC 3 cut(s) 11, 19, 147
Lsp1109I GCAGC 2 cut(s) 53, 162
LweI GCATC 2 cut(s) 243, 426
MaeI CTAG 4 cut(s) 57, 231, 350, 633
MaeIII GTNAC 1 cut(s) 483
MalI GATC 2 cut(s) 182, 340
MboI GATC 2 cut(s) 180, 338
MboII GAAGA 4 cut(s) 145, 205, 334, 536
MflI RGATCY 1 cut(s) 338
MhlI GDGCHC 2 cut(s) 16, 86
MluCI AATT 4 cut(s) 288, 578, 592, 645
MlyI GAGTC 1 cut(s) 109
MnlI CCTC 1 cut(s) 123
Mph1103I ATGCAT 1 cut(s) 441
MroXI GAANNNNTTC 2 cut(s) 72, 549
MseI TTAA 2 cut(s) 383, 661
MslI CAYNNNNRTG 3 cut(s) 166, 242, 563
MspI CCGG 2 cut(s) 61, 145
MspR9I CCNGG 2 cut(s) 145, 495
MvaI CCWGG 1 cut(s) 495
MwoI GCNNNNNNNGC 1 cut(s) 445
NciI CCSGG 1 cut(s) 145
NdeII GATC 2 cut(s) 180, 338
NlaIII CATG 4 cut(s) 302, 443, 524, 562
NlaIV GGNNCC 1 cut(s) 599
NmuCI GTSAC 1 cut(s) 483
NsiI ATGCAT 1 cut(s) 441
NspI RCATGY 2 cut(s) 302, 443
PaeI GCATGC 1 cut(s) 443
PagI TCATGA 1 cut(s) 520
PciI ACATGT 1 cut(s) 298
PdmI GAANNNNTTC 2 cut(s) 72, 549
PflMI CCANNNNNTGG 1 cut(s) 23
PkrI GCNGC 3 cut(s) 43, 152, 155
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
PscI ACATGT 1 cut(s) 298
Psp124BI GAGCTC 2 cut(s) 16, 86
Psp6I CCWGG 1 cut(s) 493
PspFI CCCAGC 1 cut(s) 462
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 1 cut(s) 599
PsuI RGATCY 1 cut(s) 338
RseI CAYNNNNRTG 3 cut(s) 166, 242, 563
SacI GAGCTC 2 cut(s) 16, 86
SaqAI TTAA 2 cut(s) 383, 661
SatI GCNGC 3 cut(s) 42, 151, 154
Sau3AI GATC 2 cut(s) 180, 338
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 2 cut(s) 145, 495
SduI GDGCHC 2 cut(s) 16, 86
SetI ASST 8 cut(s) 16, 46, 73, 86, 99, 125, 366, 490
SfaNI GCATC 2 cut(s) 243, 426
SmiMI CAYNNNNRTG 3 cut(s) 166, 242, 563
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
SphI GCATGC 1 cut(s) 443
Sse9I AATT 4 cut(s) 288, 578, 592, 645
SsiI CCGC 1 cut(s) 154
SspMI CTAG 4 cut(s) 57, 231, 350, 633
SstI GAGCTC 2 cut(s) 16, 86
StyD4I CCNGG 2 cut(s) 143, 493
TaaI ACNGT 1 cut(s) 112
TaqI TCGA 4 cut(s) 89, 107, 204, 434
TasI AATT 4 cut(s) 288, 578, 592, 645
TauI GCSGC 1 cut(s) 156
Tru1I TTAA 2 cut(s) 383, 661
Tru9I TTAA 2 cut(s) 383, 661
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 483
TseI GCWGC 2 cut(s) 41, 150
Tsp45I GTSAC 1 cut(s) 483
TspDTI ATGAA 3 cut(s) 17, 41, 537
TspRI CASTG 1 cut(s) 190
Van91I CCANNNNNTGG 1 cut(s) 23
XagI CCTNNNNNAGG 1 cut(s) 492
XapI RAATTY 1 cut(s) 288
XceI RCATGY 2 cut(s) 302, 443
XmnI GAANNNNTTC 2 cut(s) 72, 549
XspI CTAG 4 cut(s) 57, 231, 350, 633
Zsp2I ATGCAT 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.