Rmu_sc0006677.1_g000007

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006677.1
N/A
Physical Location & Seq
Forward (+)
25468 .. 26598
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 630 bp
atgccactaggaggttgcttccatttaatcaagcaaacggctagcctcctcgctaggtcagacaaggcacatagagtgtgcaatccgggacagagcccacgtcgccctaccaaaactaggcttgccgcctcttacttggcacagagtaactattggtcgttcctccaccccttcatcatccccatcgcgccgaggctatcatcgctgccacccattcgtcaccgcccgacaacctcacctcttgccatccacactccaatcttgggtcgatcagcaccagcgccactcgtctgcctcctaatcgacgccgcccttcgccagtctactctggccagtccgaccacgccgctggaccgggcattactgtcgtcggggactaaccaagcacgtctaggggccgttccgttaactactcaggagttttggattaaagtaaaaagttttcctccagcgtttctgaatgataagatggggaggaagattggtggaagtattgggaagttcattatgacttatcagaatgtgtttagtgactgtagtggaagctttctcaggttgagagtggaacttcgactttcggaaccattaaggaaaggtctaaggctgcaactggactcgagagacaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

209

Amino Acids

23.06

Weight (kDa)

11.08

Isoelectric Point (pI)

56.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 323
AccII CGCG 1 cut(s) 188
AciI CCGC 4 cut(s) 126, 223, 309, 347
AcoI YGGCCR 1 cut(s) 330
AcyI GRCGYC 1 cut(s) 306
AfiI CCNNNNNNNGG 3 cut(s) 11, 117, 263
AjiI CACGTC 2 cut(s) 101, 389
AluBI AGCT 1 cut(s) 546
AluI AGCT 1 cut(s) 546
Alw26I GTCTC 1 cut(s) 615
Ama87I CYCGRG 1 cut(s) 616
AoxI GGCC 2 cut(s) 330, 396
ApeKI GCWGC 2 cut(s) 205, 604
AspLEI GCGC 2 cut(s) 190, 283
AspS9I GGNCC 2 cut(s) 352, 396
AsuC2I CCSGG 2 cut(s) 87, 356
AsuHPI GGTGA 2 cut(s) 212, 228
AsuNHI GCTAGC 1 cut(s) 41
AvaI CYCGRG 1 cut(s) 616
AvaII GGWCC 1 cut(s) 352
BalI TGGCCA 1 cut(s) 332
BanII GRGCYC 1 cut(s) 98
BbvI GCAGC 2 cut(s) 192, 591
BccI CCATC 3 cut(s) 191, 254, 463
BceAI ACGGC 2 cut(s) 54, 383
BcgI CGANNNNNNTGC 2 cut(s) 348, 382
BcnI CCSGG 2 cut(s) 87, 356
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 5 cut(s) 8, 42, 54, 117, 392
BfmI CTRYAG 1 cut(s) 535
BfoI RGCGCY 1 cut(s) 284
BisI GCNGC 5 cut(s) 126, 206, 309, 347, 605
BlsI GCNGC 5 cut(s) 127, 207, 310, 348, 606
Bme1390I CCNGG 2 cut(s) 87, 356
Bme18I GGWCC 1 cut(s) 352
BmeT110I CYCGRG 1 cut(s) 616
BmgBI CACGTC 2 cut(s) 101, 389
BmgT120I GGNCC 2 cut(s) 352, 396
BmiI GGNNCC 2 cut(s) 397, 582
BmrFI CCNGG 2 cut(s) 87, 356
BmtI GCTAGC 1 cut(s) 45
BpmI CTGGAG 1 cut(s) 432
BpuMI CCSGG 2 cut(s) 87, 356
BsaHI GRCGYC 1 cut(s) 306
BsaJI CCNNGG 1 cut(s) 191
Bsc4I CCNNNNNNNGG 3 cut(s) 11, 117, 263
Bse1I ACTGG 3 cut(s) 319, 333, 615
BseDI CCNNGG 1 cut(s) 191
BseGI GGATG 2 cut(s) 177, 246
BseLI CCNNNNNNNGG 3 cut(s) 11, 117, 263
BseMII CTCAG 2 cut(s) 428, 565
BseNI ACTGG 3 cut(s) 319, 333, 615
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 2 cut(s) 192, 591
Bsh1236I CGCG 1 cut(s) 188
BshFI GGCC 2 cut(s) 332, 398
BsiHKCI CYCGRG 1 cut(s) 616
BsiSI CCGG 2 cut(s) 86, 355
BslFI GGGAC 2 cut(s) 102, 388
BslI CCNNNNNNNGG 3 cut(s) 11, 117, 263
BsmAI GTCTC 1 cut(s) 615
BsmFI GGGAC 2 cut(s) 102, 388
BsnI GGCC 2 cut(s) 332, 398
BsoBI CYCGRG 1 cut(s) 616
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 1 cut(s) 269
BspACI CCGC 4 cut(s) 126, 223, 309, 347
BspANI GGCC 2 cut(s) 332, 398
BspCNI CTCAG 2 cut(s) 427, 564
BspFNI CGCG 1 cut(s) 188
BspLI GGNNCC 2 cut(s) 397, 582
BspOI GCTAGC 1 cut(s) 45
BsrI ACTGG 3 cut(s) 319, 333, 615
BssECI CCNNGG 1 cut(s) 191
BssMI GATC 1 cut(s) 269
BssNI GRCGYC 1 cut(s) 306
Bst4CI ACNGT 2 cut(s) 366, 536
BstACI GRCGYC 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 43, 123
BstDEI CTNAG 3 cut(s) 414, 551, 599
BstF5I GGATG 2 cut(s) 177, 246
BstFNI CGCG 1 cut(s) 188
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 2 cut(s) 190, 283
BstKTI GATC 1 cut(s) 272
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 1 cut(s) 269
BstMWI GCNNNNNNNGC 2 cut(s) 102, 202
BstSCI CCNGG 2 cut(s) 85, 354
BstSFI CTRYAG 1 cut(s) 535
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 2 cut(s) 192, 591
BstXI CCANNNNNNTGG 1 cut(s) 349
BsuRI GGCC 2 cut(s) 332, 398
BtgZI GCGATG 2 cut(s) 169, 186
BtrI CACGTC 2 cut(s) 101, 389
BtsCI GGATG 2 cut(s) 177, 246
Cac8I GCNNGC 2 cut(s) 43, 123
CfoI GCGC 2 cut(s) 190, 283
Cfr13I GGNCC 2 cut(s) 352, 396
CseI GACGC 1 cut(s) 314
CviJI RGCY 9 cut(s) 41, 45, 96, 121, 196, 332, 398, 546, 604
CviKI_1 RGCY 9 cut(s) 41, 45, 96, 121, 196, 332, 398, 546, 604
DdeI CTNAG 3 cut(s) 414, 551, 599
DpnI GATC 1 cut(s) 271
DpnII GATC 1 cut(s) 269
EaeI YGGCCR 1 cut(s) 330
Eco24I GRGCYC 1 cut(s) 98
Eco47I GGWCC 1 cut(s) 352
Eco88I CYCGRG 1 cut(s) 616
EcoT38I GRGCYC 1 cut(s) 98
FaiI YATR 2 cut(s) 72, 509
FaqI GGGAC 2 cut(s) 102, 388
FblI GTMKAC 1 cut(s) 323
Fnu4HI GCNGC 5 cut(s) 126, 206, 309, 347, 605
FokI GGATG 2 cut(s) 164, 233
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 5 cut(s) 126, 206, 309, 347, 605
FspBI CTAG 5 cut(s) 8, 42, 54, 117, 392
GlaI GCGC 2 cut(s) 189, 282
GluI GCNGC 5 cut(s) 126, 206, 309, 347, 605
GsuI CTGGAG 1 cut(s) 432
HaeII RGCGCY 1 cut(s) 284
HaeIII GGCC 2 cut(s) 332, 398
HapII CCGG 2 cut(s) 86, 355
HgaI GACGC 1 cut(s) 314
HhaI GCGC 2 cut(s) 190, 283
Hin1I GRCGYC 1 cut(s) 306
Hin6I GCGC 2 cut(s) 188, 281
HinP1I GCGC 2 cut(s) 188, 281
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HindIII AAGCTT 1 cut(s) 544
HinfI GANTC 1 cut(s) 614
HpaI GTTAAC 1 cut(s) 408
HpaII CCGG 2 cut(s) 86, 355
HphI GGTGA 2 cut(s) 212, 228
Hpy166II GTNNAC 2 cut(s) 324, 408
Hpy188I TCNGA 5 cut(s) 61, 339, 459, 519, 580
Hpy188III TCNNGA 2 cut(s) 416, 618
Hpy8I GTNNAC 2 cut(s) 324, 408
Hpy99I CGWCG 3 cut(s) 105, 308, 373
HpyAV CCTTC 2 cut(s) 181, 323
HpyCH4III ACNGT 2 cut(s) 366, 536
HpyCH4IV ACGT 2 cut(s) 100, 388
HpyCH4V TGCA 2 cut(s) 81, 607
HpyF10VI GCNNNNNNNGC 2 cut(s) 102, 202
HpyF3I CTNAG 3 cut(s) 414, 551, 599
HpySE526I ACGT 2 cut(s) 100, 388
Hsp92I GRCGYC 1 cut(s) 306
HspAI GCGC 2 cut(s) 188, 281
KspAI GTTAAC 1 cut(s) 408
Kzo9I GATC 1 cut(s) 269
Lsp1109I GCAGC 2 cut(s) 192, 591
MaeI CTAG 5 cut(s) 8, 42, 54, 117, 392
MaeII ACGT 2 cut(s) 100, 388
MaeIII GTNAC 3 cut(s) 146, 218, 530
MalI GATC 1 cut(s) 271
MboI GATC 1 cut(s) 269
MboII GAAGA 1 cut(s) 490
MhlI GDGCHC 1 cut(s) 98
MlsI TGGCCA 1 cut(s) 332
MluNI TGGCCA 1 cut(s) 332
MlyI GAGTC 1 cut(s) 608
MmeI TCCRAC 1 cut(s) 362
Mox20I TGGCCA 1 cut(s) 332
MscI TGGCCA 1 cut(s) 332
MseI TTAA 4 cut(s) 26, 407, 429, 587
Msp20I TGGCCA 1 cut(s) 332
MspA1I CMGCKG 1 cut(s) 349
MspI CCGG 2 cut(s) 86, 355
MspR9I CCNGG 2 cut(s) 87, 356
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 2 cut(s) 102, 202
NciI CCSGG 2 cut(s) 87, 356
NdeII GATC 1 cut(s) 269
NheI GCTAGC 1 cut(s) 41
NlaIV GGNNCC 2 cut(s) 397, 582
NmeAIII GCCGAG 1 cut(s) 216
NmuCI GTSAC 2 cut(s) 218, 530
PaeR7I CTCGAG 1 cut(s) 616
PfoI TCCNGGA 1 cut(s) 85
PkrI GCNGC 5 cut(s) 127, 207, 310, 348, 606
PleI GAGTC 1 cut(s) 608
PpsI GAGTC 1 cut(s) 608
PspN4I GGNNCC 2 cut(s) 397, 582
PspPI GGNCC 2 cut(s) 352, 396
SaqAI TTAA 4 cut(s) 26, 407, 429, 587
SatI GCNGC 5 cut(s) 126, 206, 309, 347, 605
Sau3AI GATC 1 cut(s) 269
Sau96I GGNCC 2 cut(s) 352, 396
SchI GAGTC 1 cut(s) 608
ScrFI CCNGG 2 cut(s) 87, 356
SduI GDGCHC 1 cut(s) 98
SetI ASST 9 cut(s) 16, 59, 103, 236, 241, 391, 548, 557, 598
SfcI CTRYAG 1 cut(s) 535
Sfr274I CTCGAG 1 cut(s) 616
SinI GGWCC 1 cut(s) 352
SlaI CTCGAG 1 cut(s) 616
SmlI CTYRAG 1 cut(s) 616
SmoI CTYRAG 1 cut(s) 616
SsiI CCGC 4 cut(s) 126, 223, 309, 347
SspMI CTAG 5 cut(s) 8, 42, 54, 117, 392
StyD4I CCNGG 2 cut(s) 85, 354
TaaI ACNGT 2 cut(s) 366, 536
TaiI ACGT 2 cut(s) 103, 391
TaqI TCGA 4 cut(s) 268, 303, 571, 617
TauI GCSGC 3 cut(s) 128, 311, 349
Tru1I TTAA 4 cut(s) 26, 407, 429, 587
Tru9I TTAA 4 cut(s) 26, 407, 429, 587
TseFI GTSAC 2 cut(s) 218, 530
TseI GCWGC 2 cut(s) 205, 604
Tsp45I GTSAC 2 cut(s) 218, 530
TspDTI ATGAA 2 cut(s) 163, 493
TspGWI ACGGA 1 cut(s) 393
VpaK11BI GGWCC 1 cut(s) 352
XhoI CTCGAG 1 cut(s) 616
XmiI GTMKAC 1 cut(s) 323
XspI CTAG 5 cut(s) 8, 42, 54, 117, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.