Rmu_sc0006835.1_g000001

ethanolamine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006835.1
N/A
Physical Location & Seq
Reverse (-)
1 .. 1014
1014 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 399 bp
atgggagcggtgaagaacatctggaacgagagggaagtagctgaagctccagattgctctcagatccaaatccccacttcttctctctccgtcgacccttccctccctctcccccaaatcacgccgctcataacagagttgtgcaaggatctttttgagaaatggtcaaatttggacgattctcgcttctcggtcgagacggtttccggcggcatcacgaatctgttactgaaggctactgcgaaggaggaagatggaaatgacgtgtctgtaacagtcagattgtatggccctaacaccgattacgttatcaatcgcgaaagagaattgcaggcgatcaagtacctctcggccgcagggtttggtgcaaatttgcttgcagtttttggaaatggaatg

Protein Analysis

133

Amino Acids

14.5

Weight (kDa)

4.49

Isoelectric Point (pI)

25.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_kinase PF01633 65 - 133 2.1e-15 Choline/ethanolamine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 8, 127
AccI GTMKAC 1 cut(s) 93
AccII CGCG 1 cut(s) 318
AciI CCGC 4 cut(s) 8, 125, 210, 354
AclWI GGATC 2 cut(s) 58, 156
AcoI YGGCCR 1 cut(s) 351
AcsI RAATTY 2 cut(s) 169, 370
AcuI CTGAAG 2 cut(s) 63, 251
AfaI GTAC 1 cut(s) 344
AflIII ACRYGT 1 cut(s) 264
AjiI CACGTC 1 cut(s) 265
AluBI AGCT 2 cut(s) 41, 47
AluI AGCT 2 cut(s) 41, 47
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 2 cut(s) 58, 156
AoxI GGCC 2 cut(s) 289, 351
ApoI RAATTY 2 cut(s) 169, 370
AspS9I GGNCC 1 cut(s) 290
AsuHPI GGTGA 1 cut(s) 22
BccI CCATC 1 cut(s) 248
BcoDI GTCTC 1 cut(s) 191
BisI GCNGC 3 cut(s) 125, 211, 354
BlsI GCNGC 3 cut(s) 126, 212, 355
BmgBI CACGTC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 290
BmsI GCATC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 33
BsaBI GATNNNNATC 1 cut(s) 68
Bse8I GATNNNNATC 1 cut(s) 68
BseJI GATNNNNATC 1 cut(s) 68
BseMII CTCAG 1 cut(s) 74
BseX3I CGGCCG 1 cut(s) 351
Bsh1236I CGCG 1 cut(s) 318
Bsh1285I CGRYCG 2 cut(s) 195, 354
BshFI GGCC 2 cut(s) 291, 353
BsiEI CGRYCG 2 cut(s) 195, 354
BsiSI CCGG 1 cut(s) 207
BsmAI GTCTC 1 cut(s) 191
BsmBI CGTCTC 1 cut(s) 191
BsnI GGCC 2 cut(s) 291, 353
Bsp143I GATC 3 cut(s) 63, 148, 336
Bsp68I TCGCGA 1 cut(s) 318
BspACI CCGC 4 cut(s) 8, 125, 210, 354
BspANI GGCC 2 cut(s) 291, 353
BspCNI CTCAG 1 cut(s) 73
BspFNI CGCG 1 cut(s) 318
BspPI GGATC 2 cut(s) 58, 156
BsrBI CCGCTC 2 cut(s) 8, 127
BssMI GATC 3 cut(s) 63, 148, 336
Bst4CI ACNGT 2 cut(s) 202, 277
BstC8I GCNNGC 2 cut(s) 333, 378
BstDEI CTNAG 1 cut(s) 60
BstFNI CGCG 1 cut(s) 318
BstKTI GATC 3 cut(s) 66, 151, 339
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 3 cut(s) 63, 148, 336
BstMCI CGRYCG 2 cut(s) 195, 354
BstUI CGCG 1 cut(s) 318
BstX2I RGATCY 2 cut(s) 63, 148
BstYI RGATCY 2 cut(s) 63, 148
BstZI CGGCCG 1 cut(s) 351
BsuRI GGCC 2 cut(s) 291, 353
BtrI CACGTC 1 cut(s) 265
BtuMI TCGCGA 1 cut(s) 318
Cac8I GCNNGC 2 cut(s) 333, 378
Cfr13I GGNCC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 343
CviJI RGCY 5 cut(s) 41, 47, 236, 291, 353
CviKI_1 RGCY 5 cut(s) 41, 47, 236, 291, 353
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 1 cut(s) 60
DpnI GATC 3 cut(s) 65, 150, 338
DpnII GATC 3 cut(s) 63, 148, 336
EaeI YGGCCR 1 cut(s) 351
EagI CGGCCG 1 cut(s) 351
EclXI CGGCCG 1 cut(s) 351
Eco52I CGGCCG 1 cut(s) 351
Eco57I CTGAAG 2 cut(s) 63, 251
Esp3I CGTCTC 1 cut(s) 191
FaiI YATR 2 cut(s) 131, 288
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 3 cut(s) 125, 211, 354
Fsp4HI GCNGC 3 cut(s) 125, 211, 354
GluI GCNGC 3 cut(s) 125, 211, 354
GsuI CTGGAG 1 cut(s) 33
HaeIII GGCC 2 cut(s) 291, 353
HapII CCGG 1 cut(s) 207
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 2 cut(s) 179, 220
HpaII CCGG 1 cut(s) 207
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 2 cut(s) 63, 281
Hpy188III TCNNGA 5 cut(s) 22, 50, 196, 217, 317
Hpy8I GTNNAC 1 cut(s) 94
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 3 cut(s) 108, 226, 238
HpyCH4III ACNGT 2 cut(s) 202, 277
HpyCH4IV ACGT 2 cut(s) 264, 306
HpyCH4V TGCA 4 cut(s) 144, 331, 368, 380
HpyF3I CTNAG 1 cut(s) 60
HpySE526I ACGT 2 cut(s) 264, 306
Kzo9I GATC 3 cut(s) 63, 148, 336
LmnI GCTCC 2 cut(s) 5, 52
LpnPI CCDG 5 cut(s) 7, 63, 220, 317, 342
LweI GCATC 1 cut(s) 222
MaeII ACGT 2 cut(s) 264, 306
MaeIII GTNAC 2 cut(s) 225, 271
MalI GATC 3 cut(s) 65, 150, 338
MbiI CCGCTC 2 cut(s) 8, 127
MboI GATC 3 cut(s) 63, 148, 336
MboII GAAGA 3 cut(s) 25, 72, 263
MflI RGATCY 2 cut(s) 63, 148
MluCI AATT 3 cut(s) 169, 326, 370
MnlI CCTC 5 cut(s) 24, 113, 117, 241, 356
MspI CCGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 318
NdeII GATC 3 cut(s) 63, 148, 336
NmeAIII GCCGAG 1 cut(s) 329
NruI TCGCGA 1 cut(s) 318
PfeI GAWTC 2 cut(s) 179, 220
PkrI GCNGC 3 cut(s) 126, 212, 355
PspPI GGNCC 1 cut(s) 290
PsuI RGATCY 2 cut(s) 63, 148
RruI TCGCGA 1 cut(s) 318
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
SalI GTCGAC 1 cut(s) 92
SatI GCNGC 3 cut(s) 125, 211, 354
Sau3AI GATC 3 cut(s) 63, 148, 336
Sau96I GGNCC 1 cut(s) 290
SetI ASST 5 cut(s) 43, 49, 267, 309, 348
SfaNI GCATC 1 cut(s) 222
Sse9I AATT 3 cut(s) 169, 326, 370
SsiI CCGC 4 cut(s) 8, 125, 210, 354
TaaI ACNGT 2 cut(s) 202, 277
TaiI ACGT 2 cut(s) 267, 309
TaqI TCGA 2 cut(s) 93, 195
TaqII GACCGA 1 cut(s) 181
TasI AATT 3 cut(s) 169, 326, 370
TauI GCSGC 3 cut(s) 127, 213, 356
TfiI GAWTC 2 cut(s) 179, 220
TspGWI ACGGA 1 cut(s) 79
XapI RAATTY 2 cut(s) 169, 370
XmiI GTMKAC 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.