Rmu_sc0006842.1_g000005

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006842.1
N/A
Physical Location & Seq
Forward (+)
32443 .. 33600
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 771 bp
atggactactccactgatggtaatgagctctctgaacccatcttgactgacaaaagatcttcatctgttcaactagtgagctcggaactggaagaaacgctatccaacaccgagcttccgtacttccaccgacttagacgtgccacgtgggtcgaactgaagattctcttccgccttgcagctccggcagtggtggtttacttgctaaacaacgttatttccatgtccacccaaatctactgcgggcatctcggcaatctcgaactcgctgcctcttctcttggcaacaccggcatccaagtttttgcctacggcctcatgttatgttcatcttcatcttctccgagcccatcttttgatttccctaggcgaatcggccaccattgtctctgcagccgcaatcttcgtttacggacttatcccccaaatatttgcctacgcttgcaatttccccatacaaacctccaggcccagagcatcgtgttcccgagcgcgtacatatccgcggctgccctcgtggtgcatgtggtgctgagttgggtagtgatttataagttgggatggggcttgttaggcgcatcgctgatgttgagcttttcgtggtggattatagtggtggagcagtttgtttacatattgctgagtccaaggtgtaaacgtacttggacaggttttagcatgcaggctttttctgggttttggagttttctgaagctatctgtttcgtctgccgtaatgctttgcctcgagacttggtactatctgtgttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

256

Amino Acids

28.87

Weight (kDa)

8.63

Isoelectric Point (pI)

48.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AccII CGCG 2 cut(s) 494, 506
AciI CCGC 5 cut(s) 172, 243, 397, 504, 506
AclI AACGTT 1 cut(s) 213
AcoI YGGCCR 1 cut(s) 376
AcuI CTGAAG 2 cut(s) 179, 731
AcvI CACGTG 1 cut(s) 147
AfaI GTAC 4 cut(s) 122, 497, 661, 758
AgsI TTSAA 1 cut(s) 71
AhlI ACTAGT 1 cut(s) 73
AjiI CACGTC 1 cut(s) 140
AjnI CCWGG 1 cut(s) 465
AluBI AGCT 6 cut(s) 28, 81, 115, 182, 594, 715
AluI AGCT 6 cut(s) 28, 81, 115, 182, 594, 715
Alw21I GWGCWC 2 cut(s) 30, 83
Alw26I GTCTC 2 cut(s) 392, 743
Ama87I CYCGRG 2 cut(s) 487, 746
AoxI GGCC 3 cut(s) 313, 376, 468
ApeKI GCWGC 4 cut(s) 179, 269, 393, 509
AspA2I CCTAGG 1 cut(s) 365
AspLEI GCGC 2 cut(s) 494, 578
AspS9I GGNCC 1 cut(s) 469
AvaI CYCGRG 2 cut(s) 487, 746
AvrII CCTAGG 1 cut(s) 365
BanII GRGCYC 3 cut(s) 30, 83, 350
BauI CACGAG 1 cut(s) 515
BbrPI CACGTG 1 cut(s) 147
Bbv12I GWGCWC 2 cut(s) 30, 83
BbvI GCAGC 4 cut(s) 191, 256, 405, 496
BccI CCATC 4 cut(s) 11, 47, 358, 555
BceAI ACGGC 2 cut(s) 328, 716
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 1 cut(s) 467
BcoDI GTCTC 2 cut(s) 392, 743
BcuI ACTAGT 1 cut(s) 73
BfaI CTAG 2 cut(s) 74, 366
BfmI CTRYAG 1 cut(s) 391
BglII AGATCT 1 cut(s) 56
BisI GCNGC 6 cut(s) 180, 270, 394, 397, 507, 510
BlnI CCTAGG 1 cut(s) 365
BlsI GCNGC 6 cut(s) 181, 271, 395, 398, 508, 511
Bme1390I CCNGG 1 cut(s) 467
BmeT110I CYCGRG 2 cut(s) 487, 746
BmgBI CACGTC 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 469
BmrFI CCNGG 1 cut(s) 467
BmsI GCATC 4 cut(s) 256, 303, 486, 587
BpmI CTGGAG 1 cut(s) 449
BsaAI YACGTR 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 61
BsaJI CCNNGG 3 cut(s) 365, 504, 647
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 61
BseBI CCWGG 1 cut(s) 467
BseDI CCNNGG 3 cut(s) 365, 504, 647
BseGI GGATG 2 cut(s) 294, 566
BseJI GATNNNNATC 1 cut(s) 61
BseMII CTCAG 2 cut(s) 524, 632
BseNI ACTGG 1 cut(s) 93
BseXI GCAGC 4 cut(s) 191, 256, 405, 496
Bsh1236I CGCG 2 cut(s) 494, 506
BshFI GGCC 3 cut(s) 315, 378, 470
BsiHKAI GWGCWC 2 cut(s) 30, 83
BsiHKCI CYCGRG 2 cut(s) 487, 746
BsiSI CCGG 2 cut(s) 185, 291
BsmAI GTCTC 2 cut(s) 392, 743
BsnI GGCC 3 cut(s) 315, 378, 470
BsoBI CYCGRG 2 cut(s) 487, 746
Bsp1286I GDGCHC 3 cut(s) 30, 83, 350
Bsp143I GATC 1 cut(s) 56
BspACI CCGC 5 cut(s) 172, 243, 397, 504, 506
BspANI GGCC 3 cut(s) 315, 378, 470
BspCNI CTCAG 2 cut(s) 525, 633
BspFNI CGCG 2 cut(s) 494, 506
BspMAI CTGCAG 1 cut(s) 395
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 1 cut(s) 93
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 3 cut(s) 365, 504, 647
BssMI GATC 1 cut(s) 56
BssSI CACGAG 1 cut(s) 515
BssT1I CCWWGG 2 cut(s) 365, 647
Bst2BI CACGAG 1 cut(s) 515
Bst2UI CCWGG 1 cut(s) 467
Bst6I CTCTTC 2 cut(s) 173, 280
BstAPI GCANNNNNTGC 1 cut(s) 529
BstBAI YACGTR 1 cut(s) 147
BstC8I GCNNGC 4 cut(s) 245, 443, 680, 684
BstDEI CTNAG 3 cut(s) 134, 533, 641
BstDSI CCRYGG 1 cut(s) 504
BstF5I GGATG 2 cut(s) 294, 566
BstFNI CGCG 2 cut(s) 494, 506
BstHHI GCGC 2 cut(s) 494, 578
BstKTI GATC 1 cut(s) 59
BstMAI GTCTC 2 cut(s) 392, 743
BstMBI GATC 1 cut(s) 56
BstMWI GCNNNNNNNGC 4 cut(s) 185, 291, 529, 573
BstNI CCWGG 1 cut(s) 467
BstNSI RCATGY 2 cut(s) 527, 682
BstSCI CCNGG 1 cut(s) 465
BstSFI CTRYAG 1 cut(s) 391
BstUI CGCG 2 cut(s) 494, 506
BstV1I GCAGC 4 cut(s) 191, 256, 405, 496
BstX2I RGATCY 1 cut(s) 56
BstYI RGATCY 1 cut(s) 56
BsuRI GGCC 3 cut(s) 315, 378, 470
BtgI CCRYGG 1 cut(s) 504
BtgZI GCGATG 1 cut(s) 564
BtrI CACGTC 1 cut(s) 140
BtsCI GGATG 2 cut(s) 294, 566
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 12, 195
Cac8I GCNNGC 4 cut(s) 245, 443, 680, 684
CfoI GCGC 2 cut(s) 494, 578
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 469
Cfr42I CCGCGG 1 cut(s) 507
Csp6I GTAC 4 cut(s) 121, 496, 660, 757
CviAII CATG 4 cut(s) 223, 319, 524, 679
CviQI GTAC 4 cut(s) 121, 496, 660, 757
DdeI CTNAG 3 cut(s) 134, 533, 641
DpnI GATC 1 cut(s) 58
DpnII GATC 1 cut(s) 56
EaeI YGGCCR 1 cut(s) 376
Eam1104I CTCTTC 2 cut(s) 173, 280
EarI CTCTTC 2 cut(s) 173, 280
EciI GGCGGA 1 cut(s) 161
Ecl136II GAGCTC 2 cut(s) 28, 81
Eco130I CCWWGG 2 cut(s) 365, 647
Eco24I GRGCYC 3 cut(s) 30, 83, 350
Eco53kI GAGCTC 2 cut(s) 28, 81
Eco57I CTGAAG 2 cut(s) 179, 731
Eco72I CACGTG 1 cut(s) 147
Eco88I CYCGRG 2 cut(s) 487, 746
EcoICRI GAGCTC 2 cut(s) 28, 81
EcoRII CCWGG 1 cut(s) 465
EcoT14I CCWWGG 2 cut(s) 365, 647
EcoT38I GRGCYC 3 cut(s) 30, 83, 350
ErhI CCWWGG 2 cut(s) 365, 647
FaeI CATG 4 cut(s) 226, 322, 527, 682
FalI AAGNNNNNCTT 2 cut(s) 152, 184
FatI CATG 4 cut(s) 222, 318, 523, 678
FauI CCCGC 1 cut(s) 236
Fnu4HI GCNGC 6 cut(s) 180, 270, 394, 397, 507, 510
FokI GGATG 2 cut(s) 281, 573
FriOI GRGCYC 3 cut(s) 30, 83, 350
Fsp4HI GCNGC 6 cut(s) 180, 270, 394, 397, 507, 510
FspBI CTAG 2 cut(s) 74, 366
GlaI GCGC 2 cut(s) 493, 577
GluI GCNGC 6 cut(s) 180, 270, 394, 397, 507, 510
GsuI CTGGAG 1 cut(s) 449
HaeIII GGCC 3 cut(s) 315, 378, 470
HapII CCGG 2 cut(s) 185, 291
HhaI GCGC 2 cut(s) 494, 578
Hin1II CATG 4 cut(s) 226, 322, 527, 682
Hin6I GCGC 2 cut(s) 492, 576
HinP1I GCGC 2 cut(s) 492, 576
HinfI GANTC 3 cut(s) 163, 372, 643
HpaII CCGG 2 cut(s) 185, 291
Hpy166II GTNNAC 5 cut(s) 199, 228, 410, 631, 656
Hpy188I TCNGA 4 cut(s) 34, 85, 345, 711
Hpy188III TCNNGA 4 cut(s) 43, 260, 487, 748
Hpy8I GTNNAC 5 cut(s) 199, 228, 410, 631, 656
HpyCH4IV ACGT 4 cut(s) 139, 146, 213, 658
HpyCH4V TGCA 5 cut(s) 179, 393, 445, 523, 682
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 291, 529, 573
HpyF3I CTNAG 3 cut(s) 134, 533, 641
HpySE526I ACGT 4 cut(s) 139, 146, 213, 658
Hsp92II CATG 4 cut(s) 226, 322, 527, 682
HspAI GCGC 2 cut(s) 492, 576
KspI CCGCGG 1 cut(s) 507
Kzo9I GATC 1 cut(s) 56
LmnI GCTCC 2 cut(s) 187, 619
LpnPI CCDG 9 cut(s) 74, 198, 304, 452, 479, 485, 654, 668, 678
Lsp1109I GCAGC 4 cut(s) 191, 256, 405, 496
LweI GCATC 4 cut(s) 256, 303, 486, 587
MaeI CTAG 2 cut(s) 74, 366
MaeII ACGT 4 cut(s) 139, 146, 213, 658
MalI GATC 1 cut(s) 58
MboI GATC 1 cut(s) 56
MboII GAAGA 8 cut(s) 51, 104, 160, 172, 267, 324, 330, 395
MflI RGATCY 1 cut(s) 56
MhlI GDGCHC 3 cut(s) 30, 83, 350
MluCI AATT 1 cut(s) 446
MlyI GAGTC 1 cut(s) 652
MmeI TCCRAC 1 cut(s) 129
MnlI CCTC 5 cut(s) 283, 326, 473, 524, 755
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 2 cut(s) 185, 291
MspR9I CCNGG 1 cut(s) 467
MvaI CCWGG 1 cut(s) 467
MvnI CGCG 2 cut(s) 494, 506
MwoI GCNNNNNNNGC 4 cut(s) 185, 291, 529, 573
NdeII GATC 1 cut(s) 56
NlaIII CATG 4 cut(s) 226, 322, 527, 682
NmeAIII GCCGAG 1 cut(s) 231
NspI RCATGY 2 cut(s) 527, 682
PaeI GCATGC 1 cut(s) 682
PaeR7I CTCGAG 1 cut(s) 746
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PfeI GAWTC 2 cut(s) 163, 372
PkrI GCNGC 6 cut(s) 181, 271, 395, 398, 508, 511
PleI GAGTC 1 cut(s) 651
PmaCI CACGTG 1 cut(s) 147
PmlI CACGTG 1 cut(s) 147
PpsI GAGTC 1 cut(s) 651
Ppu21I YACGTR 1 cut(s) 147
PsiI TTATAA 1 cut(s) 552
Psp124BI GAGCTC 2 cut(s) 30, 83
Psp1406I AACGTT 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 465
PspCI CACGTG 1 cut(s) 147
PspGI CCWGG 1 cut(s) 465
PspPI GGNCC 1 cut(s) 469
PstI CTGCAG 1 cut(s) 395
PsuI RGATCY 1 cut(s) 56
RsaI GTAC 4 cut(s) 122, 497, 661, 758
RsaNI GTAC 4 cut(s) 121, 496, 660, 757
SacI GAGCTC 2 cut(s) 30, 83
SacII CCGCGG 1 cut(s) 507
SatI GCNGC 6 cut(s) 180, 270, 394, 397, 507, 510
Sau3AI GATC 1 cut(s) 56
Sau96I GGNCC 1 cut(s) 469
SchI GAGTC 1 cut(s) 652
ScrFI CCNGG 1 cut(s) 467
SduI GDGCHC 3 cut(s) 30, 83, 350
SfaNI GCATC 4 cut(s) 256, 303, 486, 587
SfcI CTRYAG 1 cut(s) 391
Sfr274I CTCGAG 1 cut(s) 746
Sfr303I CCGCGG 1 cut(s) 507
SgrBI CCGCGG 1 cut(s) 507
SlaI CTCGAG 1 cut(s) 746
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
SpeI ACTAGT 1 cut(s) 73
SphI GCATGC 1 cut(s) 682
Sse9I AATT 1 cut(s) 446
SsiI CCGC 5 cut(s) 172, 243, 397, 504, 506
SspI AATATT 1 cut(s) 430
SspMI CTAG 2 cut(s) 74, 366
SstI GAGCTC 2 cut(s) 30, 83
StyD4I CCNGG 1 cut(s) 465
StyI CCWWGG 2 cut(s) 365, 647
TaiI ACGT 4 cut(s) 142, 149, 216, 661
TaqI TCGA 3 cut(s) 153, 261, 747
TasI AATT 1 cut(s) 446
TauI GCSGC 2 cut(s) 399, 509
TfiI GAWTC 2 cut(s) 163, 372
TscAI CASTG 2 cut(s) 19, 195
TseI GCWGC 4 cut(s) 179, 269, 393, 509
TspDTI ATGAA 3 cut(s) 51, 318, 324
TspGWI ACGGA 2 cut(s) 108, 427
TspRI CASTG 2 cut(s) 19, 195
XceI RCATGY 2 cut(s) 527, 682
XhoI CTCGAG 1 cut(s) 746
XmaJI CCTAGG 1 cut(s) 365
XspI CTAG 2 cut(s) 74, 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.