Rmu_sc0007199.1_g000010

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007199.1
N/A
Physical Location & Seq
Reverse (-)
49808 .. 52268
2461 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 939 bp
atggcggcgccggcgtacgaggcgactggaggagcggtgccggactggctgaacaaaggtgacaacgcatggcagatgatctccgccacgctagtgggtctccagagcgtgccggggctggtcatcctctacgggagcatagtgaagaagaagtgggcggtcaactccgccttcatggccctctatgccttcgccgccgtcgtgctttgctgggtgtcgtgggcctacaagatgtcgttcggggacgagctgctgccgttttgggggaaggccgggcctgccttagggcagaagtttctgctcaagcacgctgccctccccgaatccacacagcaccacgccgacggcagtattgagactcccatggccatgccgttatacccgatggcgaccatggtgtggttccagtgcgtgttcgcggccataacactgatactgttggcagggtcggtgctggggagaatgaacttcaaggcgtggatgatgttcgtgcctctgtggctgacgttcgcgtacaccgtcggtgcttttagcttgtggggcggagggttcttgttccactggggtgttatggattactccggtgggtacgtcattcatctttcttcagggattgctggtttcaccactgcttactggctgttttgccttggcagagtccttctgaagaggaacagaatcctagtgctagatgaggctactgcttccatcgattctgcaacagacgccgttctacaaagaatcatcaggcaggaatttgcggaatgcacagtgatgacggttgctcatagggttccaacagttatagatagtgacatggtcatggtcctctcctatgggaagctagtggagtatgaagagccttcaaagctcttggataccaactcatacttctccaagcttgtagctgaatattggtcaagctgtagaggaaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

312

Amino Acids

34.46

Weight (kDa)

6.58

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 23 - 214 1e-26 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 7, 37
AccB7I CCANNNNNTGG 1 cut(s) 399
AccBSI CCGCTC 1 cut(s) 35
AccII CGCG 2 cut(s) 419, 512
AciI CCGC 9 cut(s) 5, 35, 84, 158, 168, 195, 419, 543, 761
AcoI YGGCCR 2 cut(s) 366, 420
AcsI RAATTY 1 cut(s) 755
AcuI CTGAAG 2 cut(s) 591, 686
AcyI GRCGYC 2 cut(s) 8, 726
AfaI GTAC 3 cut(s) 17, 515, 590
AfiI CCNNNNNNNGG 3 cut(s) 263, 284, 399
AgsI TTSAA 2 cut(s) 472, 867
AjuI GAANNNNNNNTTGG 2 cut(s) 875, 907
AleI CACNNNNGTG 2 cut(s) 92, 564
AluBI AGCT 7 cut(s) 250, 534, 844, 871, 901, 908, 924
AluI AGCT 7 cut(s) 250, 534, 844, 871, 901, 908, 924
Alw26I GTCTC 2 cut(s) 104, 350
AoxI GGCC 6 cut(s) 177, 222, 270, 275, 366, 420
ApeKI GCWGC 3 cut(s) 250, 253, 311
ApoI RAATTY 1 cut(s) 755
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 4 cut(s) 178, 222, 275, 826
AsuC2I CCSGG 2 cut(s) 114, 274
AsuHPI GGTGA 2 cut(s) 71, 616
AvaII GGWCC 1 cut(s) 826
AxyI CCTNAGG 1 cut(s) 283
BalI TGGCCA 1 cut(s) 368
BanI GGYRCC 2 cut(s) 7, 37
BbvI GCAGC 3 cut(s) 237, 240, 298
BccI CCATC 2 cut(s) 379, 716
BceAI ACGGC 5 cut(s) 182, 241, 358, 361, 713
BciVI GTATCC 1 cut(s) 871
BcnI CCSGG 2 cut(s) 114, 274
BcoDI GTCTC 2 cut(s) 104, 350
BfaI CTAG 4 cut(s) 92, 683, 689, 845
BfmI CTRYAG 1 cut(s) 925
BfoI RGCGCY 1 cut(s) 11
BfuI GTATCC 1 cut(s) 871
BglI GCCNNNNNGGC 3 cut(s) 46, 176, 499
BisI GCNGC 6 cut(s) 6, 195, 251, 254, 312, 420
BlsI GCNGC 6 cut(s) 7, 196, 252, 255, 313, 421
Bme1390I CCNGG 2 cut(s) 114, 274
Bme18I GGWCC 1 cut(s) 826
BmgT120I GGNCC 4 cut(s) 178, 222, 275, 826
BmiI GGNNCC 4 cut(s) 9, 39, 404, 795
BmrFI CCNGG 2 cut(s) 114, 274
BmrI ACTGGG 1 cut(s) 571
BmuI ACTGGG 1 cut(s) 571
BpmI CTGGAG 2 cut(s) 48, 86
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 2 cut(s) 114, 274
Bsa29I ATCGAT 1 cut(s) 711
BsaHI GRCGYC 2 cut(s) 8, 726
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 4 cut(s) 113, 363, 393, 649
BsaWI WCCGGW 1 cut(s) 581
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 3 cut(s) 263, 284, 399
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 5 cut(s) 31, 50, 406, 566, 641
Bse21I CCTNAGG 1 cut(s) 283
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 4 cut(s) 113, 363, 393, 649
BseGI GGATG 2 cut(s) 123, 486
BseLI CCNNNNNNNGG 3 cut(s) 263, 284, 399
BseNI ACTGG 5 cut(s) 31, 50, 406, 566, 641
BseRI GAGGAG 1 cut(s) 45
BseXI GCAGC 3 cut(s) 237, 240, 298
BseYI CCCAGC 2 cut(s) 210, 454
Bsh1236I CGCG 2 cut(s) 419, 512
BshFI GGCC 6 cut(s) 179, 224, 272, 277, 368, 422
BshNI GGYRCC 2 cut(s) 7, 37
BshVI ATCGAT 1 cut(s) 711
BsiSI CCGG 5 cut(s) 11, 41, 113, 273, 582
BsiWI CGTACG 1 cut(s) 15
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 3 cut(s) 263, 284, 399
BsmAI GTCTC 2 cut(s) 104, 350
BsmFI GGGAC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 770
BsnI GGCC 6 cut(s) 179, 224, 272, 277, 368, 422
Bso31I GGTCTC 1 cut(s) 104
Bsp143I GATC 1 cut(s) 78
Bsp19I CCATGG 2 cut(s) 363, 393
BspACI CCGC 9 cut(s) 5, 35, 84, 158, 168, 195, 419, 543, 761
BspANI GGCC 6 cut(s) 179, 224, 272, 277, 368, 422
BspDI ATCGAT 1 cut(s) 711
BspFNI CGCG 2 cut(s) 419, 512
BspLI GGNNCC 4 cut(s) 9, 39, 404, 795
BspQI GCTCTTC 1 cut(s) 852
BspT107I GGYRCC 2 cut(s) 7, 37
BspTNI GGTCTC 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 35
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 5 cut(s) 31, 50, 406, 566, 641
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 4 cut(s) 113, 363, 393, 649
BssMI GATC 1 cut(s) 78
BssNI GRCGYC 2 cut(s) 8, 726
BssT1I CCWWGG 3 cut(s) 363, 393, 649
Bst4CI ACNGT 5 cut(s) 438, 520, 772, 781, 802
Bst6I CTCTTC 2 cut(s) 662, 852
BstACI GRCGYC 2 cut(s) 8, 726
BstC8I GCNNGC 4 cut(s) 12, 110, 279, 309
BstDEI CTNAG 1 cut(s) 283
BstDSI CCRYGG 2 cut(s) 363, 393
BstENI CCTNNNNNAGG 1 cut(s) 282
BstF5I GGATG 2 cut(s) 123, 486
BstFNI CGCG 2 cut(s) 419, 512
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 1 cut(s) 81
BstMAI GTCTC 2 cut(s) 104, 350
BstMBI GATC 1 cut(s) 78
BstSCI CCNGG 2 cut(s) 112, 272
BstSFI CTRYAG 1 cut(s) 925
BstUI CGCG 2 cut(s) 419, 512
BstV1I GCAGC 3 cut(s) 237, 240, 298
BstXI CCANNNNNNTGG 1 cut(s) 94
Bsu15I ATCGAT 1 cut(s) 711
Bsu36I CCTNAGG 1 cut(s) 283
BsuI GTATCC 1 cut(s) 871
BsuRI GGCC 6 cut(s) 179, 224, 272, 277, 368, 422
BsuTUI ATCGAT 1 cut(s) 711
BtgI CCRYGG 2 cut(s) 363, 393
BtsCI GGATG 2 cut(s) 123, 486
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 5 cut(s) 413, 428, 559, 627, 777
Cac8I GCNNGC 4 cut(s) 12, 110, 279, 309
CfoI GCGC 1 cut(s) 10
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 4 cut(s) 178, 222, 275, 826
ClaI ATCGAT 1 cut(s) 711
CseI GACGC 1 cut(s) 734
Csp6I GTAC 3 cut(s) 16, 514, 589
CviAII CATG 7 cut(s) 69, 175, 364, 370, 394, 817, 823
CviQI GTAC 3 cut(s) 16, 514, 589
DdeI CTNAG 1 cut(s) 283
DinI GGCGCC 1 cut(s) 9
DpnI GATC 1 cut(s) 80
DpnII GATC 1 cut(s) 78
EaeI YGGCCR 2 cut(s) 366, 420
Eam1104I CTCTTC 2 cut(s) 662, 852
EarI CTCTTC 2 cut(s) 662, 852
EciI GGCGGA 3 cut(s) 73, 157, 558
Eco130I CCWWGG 3 cut(s) 363, 393, 649
Eco31I GGTCTC 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 826
Eco57I CTGAAG 2 cut(s) 591, 686
Eco81I CCTNAGG 1 cut(s) 283
EcoNI CCTNNNNNAGG 1 cut(s) 282
EcoT14I CCWWGG 3 cut(s) 363, 393, 649
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
ErhI CCWWGG 3 cut(s) 363, 393, 649
FaeI CATG 7 cut(s) 72, 178, 367, 373, 397, 820, 826
FaqI GGGAC 1 cut(s) 257
FatI CATG 7 cut(s) 68, 174, 363, 369, 393, 816, 822
Fnu4HI GCNGC 6 cut(s) 6, 195, 251, 254, 312, 420
FokI GGATG 2 cut(s) 110, 493
Fsp4HI GCNGC 6 cut(s) 6, 195, 251, 254, 312, 420
FspBI CTAG 4 cut(s) 92, 683, 689, 845
GlaI GCGC 1 cut(s) 9
GluI GCNGC 6 cut(s) 6, 195, 251, 254, 312, 420
GsaI CCCAGC 2 cut(s) 214, 458
GsuI CTGGAG 2 cut(s) 48, 86
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 6 cut(s) 179, 224, 272, 277, 368, 422
HapII CCGG 5 cut(s) 11, 41, 113, 273, 582
HgaI GACGC 1 cut(s) 734
HhaI GCGC 1 cut(s) 10
Hin1I GRCGYC 2 cut(s) 8, 726
Hin1II CATG 7 cut(s) 72, 178, 367, 373, 397, 820, 826
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HindIII AAGCTT 1 cut(s) 899
HinfI GANTC 6 cut(s) 323, 358, 657, 678, 713, 741
HpaII CCGG 5 cut(s) 11, 41, 113, 273, 582
HphI GGTGA 2 cut(s) 71, 616
Hpy166II GTNNAC 2 cut(s) 163, 516
Hpy188I TCNGA 1 cut(s) 666
Hpy188III TCNNGA 1 cut(s) 103
Hpy8I GTNNAC 2 cut(s) 163, 516
Hpy99I CGWCG 3 cut(s) 203, 347, 524
HpyAV CCTTC 5 cut(s) 181, 199, 262, 671, 873
HpyCH4III ACNGT 5 cut(s) 438, 520, 772, 781, 802
HpyCH4IV ACGT 2 cut(s) 506, 591
HpyCH4V TGCA 2 cut(s) 719, 768
HpyF3I CTNAG 1 cut(s) 283
HpySE526I ACGT 2 cut(s) 506, 591
Hsp92I GRCGYC 2 cut(s) 8, 726
Hsp92II CATG 7 cut(s) 72, 178, 367, 373, 397, 820, 826
HspAI GCGC 1 cut(s) 8
KasI GGCGCC 1 cut(s) 7
KroI GCCGGC 1 cut(s) 10
KroNI GCCGGC 1 cut(s) 12
Kzo9I GATC 1 cut(s) 78
LguI GCTCTTC 1 cut(s) 852
LmnI GCTCC 2 cut(s) 32, 135
Lsp1109I GCAGC 3 cut(s) 237, 240, 298
MaeI CTAG 4 cut(s) 92, 683, 689, 845
MaeII ACGT 2 cut(s) 506, 591
MaeIII GTNAC 2 cut(s) 59, 812
MalI GATC 1 cut(s) 80
MbiI CCGCTC 1 cut(s) 35
MboI GATC 1 cut(s) 78
MboII GAAGA 5 cut(s) 157, 160, 597, 679, 869
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 1 cut(s) 755
MluNI TGGCCA 1 cut(s) 368
Mly113I GGCGCC 1 cut(s) 8
MlyI GAGTC 2 cut(s) 352, 666
MmeI TCCRAC 1 cut(s) 821
Mox20I TGGCCA 1 cut(s) 368
MreI CGCCGGCG 1 cut(s) 10
MroNI GCCGGC 1 cut(s) 10
MscI TGGCCA 1 cut(s) 368
MslI CAYNNNNRTG 5 cut(s) 92, 368, 564, 773, 821
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 5 cut(s) 11, 41, 113, 273, 582
MspR9I CCNGG 2 cut(s) 114, 274
Mva1269I GAATGC 1 cut(s) 770
MvnI CGCG 2 cut(s) 419, 512
NaeI GCCGGC 1 cut(s) 12
NarI GGCGCC 1 cut(s) 8
NciI CCSGG 2 cut(s) 114, 274
NcoI CCATGG 2 cut(s) 363, 393
NdeII GATC 1 cut(s) 78
NgoMIV GCCGGC 1 cut(s) 10
NlaIII CATG 7 cut(s) 72, 178, 367, 373, 397, 820, 826
NlaIV GGNNCC 4 cut(s) 9, 39, 404, 795
NmuCI GTSAC 2 cut(s) 59, 812
OliI CACNNNNGTG 2 cut(s) 92, 564
PciSI GCTCTTC 1 cut(s) 852
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 516
PctI GAATGC 1 cut(s) 770
PdiI GCCGGC 1 cut(s) 12
PfeI GAWTC 4 cut(s) 323, 678, 713, 741
Pfl23II CGTACG 1 cut(s) 15
PflFI GACNNNGTC 1 cut(s) 818
PflMI CCANNNNNTGG 1 cut(s) 399
PkrI GCNGC 6 cut(s) 7, 196, 252, 255, 313, 421
PleI GAGTC 2 cut(s) 352, 665
PluTI GGCGCC 1 cut(s) 11
PpsI GAGTC 2 cut(s) 352, 665
PspFI CCCAGC 2 cut(s) 210, 454
PspLI CGTACG 1 cut(s) 15
PspN4I GGNNCC 4 cut(s) 9, 39, 404, 795
PspPI GGNCC 4 cut(s) 178, 222, 275, 826
PsyI GACNNNGTC 1 cut(s) 818
RsaI GTAC 3 cut(s) 17, 515, 590
RsaNI GTAC 3 cut(s) 16, 514, 589
RseI CAYNNNNRTG 5 cut(s) 92, 368, 564, 773, 821
SapI GCTCTTC 1 cut(s) 852
SatI GCNGC 6 cut(s) 6, 195, 251, 254, 312, 420
Sau3AI GATC 1 cut(s) 78
Sau96I GGNCC 4 cut(s) 178, 222, 275, 826
SchI GAGTC 2 cut(s) 352, 666
ScrFI CCNGG 2 cut(s) 114, 274
SfcI CTRYAG 1 cut(s) 925
SfoI GGCGCC 1 cut(s) 9
SgrAI CRCCGGYG 1 cut(s) 10
SinI GGWCC 1 cut(s) 826
SmiMI CAYNNNNRTG 5 cut(s) 92, 368, 564, 773, 821
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 1 cut(s) 755
SsiI CCGC 9 cut(s) 5, 35, 84, 158, 168, 195, 419, 543, 761
SspDI GGCGCC 1 cut(s) 7
SspI AATATT 1 cut(s) 914
SspMI CTAG 4 cut(s) 92, 683, 689, 845
StyD4I CCNGG 2 cut(s) 112, 272
StyI CCWWGG 3 cut(s) 363, 393, 649
TaaI ACNGT 5 cut(s) 438, 520, 772, 781, 802
TaiI ACGT 2 cut(s) 509, 594
TaqI TCGA 1 cut(s) 711
TasI AATT 1 cut(s) 755
TauI GCSGC 3 cut(s) 8, 197, 422
TfiI GAWTC 4 cut(s) 323, 678, 713, 741
TscAI CASTG 5 cut(s) 413, 435, 566, 634, 777
TseFI GTSAC 2 cut(s) 59, 812
TseI GCWGC 3 cut(s) 250, 253, 311
Tsp45I GTSAC 2 cut(s) 59, 812
TspDTI ATGAA 4 cut(s) 163, 479, 587, 870
TspRI CASTG 5 cut(s) 413, 435, 566, 634, 777
Tth111I GACNNNGTC 1 cut(s) 818
Van91I CCANNNNNTGG 1 cut(s) 399
VpaK11BI GGWCC 1 cut(s) 826
XagI CCTNNNNNAGG 1 cut(s) 282
XapI RAATTY 1 cut(s) 755
XspI CTAG 4 cut(s) 92, 683, 689, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.