Rmu_sc0007224.1_g000001

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007224.1
N/A
Physical Location & Seq
Reverse (-)
2 .. 2194
2193 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 806 bp
atggtcataggcactgtcaagaagggtaagcctgatctgatgacgaaggtcctgccagctgtcatttggaggcagcgcaagccatggcaccaaaaggatggtgtcttcatgtgcttcgaagataatgctggtgttagtgttaatccgaagggagaaatgaaggatctgcaattactggtcttattgggaggagtgggaacccgaaggcttcctagactcaaaataaaccccttcggcgcatgtgaaatgctccaactgtgcattgcagcacagtgcctcgccactttgacagcttcaatagcagagtccgagtggaacattttgaatcagagaaccaggaatgggatgaagatcagcttgaagaattactgtgagagctggaggatgaatgtagagctccacaacattagagagttccaagtggtgcctgaggagtgcataggatacgttggaaaatatgttacttcgactcagtacaaggtggactcagaaagggctattgaggagtccattgtgtatctaagcactagctgtaatttgaagaatgatggtagagatgtatggatttttgacattgatgatacactgctctctactgtgccttactacaggggacatcatttcgggggtgagaagttgaacctgactgctttggaggagtggatgagccatggcaaggcacctgctttggaacactcgctcaaactcttcaatgagatcaaagccagagggctgcatatcattttggtttcttcaaggagggagcatcttagatcagccacaataaacaaccttctccatgttgg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

269

Amino Acids

30.54

Weight (kDa)

8.78

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L14 PF00238 1 - 54 4.4e-10 Ribosomal protein L14p/L23e
Acid_phosphat_B PF03767 118 - 269 3.5e-45 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 691
Acc36I ACCTGC 1 cut(s) 691
AccB1I GGYRCC 3 cut(s) 87, 424, 679
AclWI GGATC 1 cut(s) 171
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 2 cut(s) 342, 676
AgsI TTSAA 7 cut(s) 297, 325, 361, 541, 640, 712, 756
AjnI CCWGG 1 cut(s) 335
AluBI AGCT 6 cut(s) 59, 293, 357, 378, 397, 531
AluI AGCT 6 cut(s) 59, 293, 357, 378, 397, 531
Alw21I GWGCWC 1 cut(s) 399
AlwI GGATC 1 cut(s) 171
ApeKI GCWGC 3 cut(s) 73, 266, 733
AspLEI GCGC 2 cut(s) 78, 239
AspS9I GGNCC 1 cut(s) 49
AsuHPI GGTGA 1 cut(s) 641
AsuII TTCGAA 1 cut(s) 117
AvaII GGWCC 1 cut(s) 49
AxyI CCTNAGG 1 cut(s) 429
BanI GGYRCC 3 cut(s) 87, 424, 679
BanII GRGCYC 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 97
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 3 cut(s) 85, 278, 720
BccI CCATC 2 cut(s) 92, 542
BcgI CGANNNNNNTGC 4 cut(s) 34, 68, 107, 141
BciT130I CCWGG 1 cut(s) 337
BciVI GTATCC 1 cut(s) 437
BfaI CTAG 2 cut(s) 213, 528
BfmI CTRYAG 1 cut(s) 607
BfuAI ACCTGC 1 cut(s) 691
BfuI GTATCC 1 cut(s) 437
BisI GCNGC 3 cut(s) 74, 267, 734
BlsI GCNGC 3 cut(s) 75, 268, 735
Bme1390I CCNGG 1 cut(s) 337
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 4 cut(s) 89, 199, 426, 681
BmrFI CCNGG 1 cut(s) 337
BmsI GCATC 1 cut(s) 775
BoxI GACNNNNGTC 1 cut(s) 47
BpiI GAAGAC 1 cut(s) 97
BpmI CTGGAG 1 cut(s) 400
Bpu14I TTCGAA 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 350
BsaJI CCNNGG 2 cut(s) 83, 670
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 676
Bse1I ACTGG 1 cut(s) 180
Bse21I CCTNAGG 1 cut(s) 429
Bse3DI GCAATG 1 cut(s) 261
Bse8I GATNNNNATC 1 cut(s) 350
BseBI CCWGG 1 cut(s) 337
BseDI CCNNGG 2 cut(s) 83, 670
BseGI GGATG 4 cut(s) 103, 351, 390, 669
BseJI GATNNNNATC 1 cut(s) 350
BseLI CCNNNNNNNGG 2 cut(s) 342, 676
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 3 cut(s) 420, 485, 501
BseNI ACTGG 1 cut(s) 180
BseRI GAGGAG 4 cut(s) 204, 446, 518, 671
BseXI GCAGC 3 cut(s) 85, 278, 720
BshNI GGYRCC 3 cut(s) 87, 424, 679
BsiHKAI GWGCWC 1 cut(s) 399
BslFI GGGAC 1 cut(s) 627
BslI CCNNNNNNNGG 2 cut(s) 342, 676
BsmFI GGGAC 1 cut(s) 627
Bsp119I TTCGAA 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 5 cut(s) 34, 163, 351, 717, 773
Bsp19I CCATGG 2 cut(s) 83, 670
BspCNI CTCAG 3 cut(s) 421, 484, 500
BspLI GGNNCC 4 cut(s) 89, 199, 426, 681
BspMI ACCTGC 1 cut(s) 691
BspPI GGATC 1 cut(s) 171
BspT104I TTCGAA 1 cut(s) 117
BspT107I GGYRCC 3 cut(s) 87, 424, 679
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 1 cut(s) 180
BssECI CCNNGG 2 cut(s) 83, 670
BssMI GATC 5 cut(s) 34, 163, 351, 717, 773
BssT1I CCWWGG 2 cut(s) 83, 670
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 5 cut(s) 16, 258, 273, 371, 598
Bst6I CTCTTC 1 cut(s) 713
BstBI TTCGAA 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 57, 80
BstDEI CTNAG 5 cut(s) 429, 471, 487, 521, 770
BstDSI CCRYGG 2 cut(s) 83, 670
BstF5I GGATG 4 cut(s) 103, 351, 390, 669
BstHHI GCGC 2 cut(s) 78, 239
BstKTI GATC 5 cut(s) 37, 166, 354, 720, 776
BstMBI GATC 5 cut(s) 34, 163, 351, 717, 773
BstMWI GCNNNNNNNGC 2 cut(s) 79, 299
BstNI CCWGG 1 cut(s) 337
BstNSI RCATGY 1 cut(s) 243
BstPAI GACNNNNGTC 1 cut(s) 47
BstSCI CCNGG 1 cut(s) 335
BstSFI CTRYAG 1 cut(s) 607
BstV1I GCAGC 3 cut(s) 85, 278, 720
BstV2I GAAGAC 1 cut(s) 97
BstX2I RGATCY 1 cut(s) 163
BstXI CCANNNNNNTGG 1 cut(s) 98
BstYI RGATCY 1 cut(s) 163
Bsu36I CCTNAGG 1 cut(s) 429
BsuI GTATCC 1 cut(s) 437
BtgI CCRYGG 2 cut(s) 83, 670
BtsCI GGATG 4 cut(s) 103, 351, 390, 669
BtsI GCAGTG 1 cut(s) 584
BtsIMutI CAGTG 3 cut(s) 12, 278, 584
BveI ACCTGC 1 cut(s) 691
Cac8I GCNNGC 2 cut(s) 57, 80
CfoI GCGC 2 cut(s) 78, 239
Cfr13I GGNCC 1 cut(s) 49
Csp6I GTAC 1 cut(s) 475
CviAII CATG 5 cut(s) 84, 109, 240, 671, 800
CviQI GTAC 1 cut(s) 475
DdeI CTNAG 5 cut(s) 429, 471, 487, 521, 770
DpnI GATC 5 cut(s) 36, 165, 353, 719, 775
DpnII GATC 5 cut(s) 34, 163, 351, 717, 773
Eam1104I CTCTTC 1 cut(s) 713
EarI CTCTTC 1 cut(s) 713
Ecl136II GAGCTC 1 cut(s) 397
Eco130I CCWWGG 2 cut(s) 83, 670
Eco24I GRGCYC 1 cut(s) 399
Eco47I GGWCC 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 397
Eco81I CCTNAGG 1 cut(s) 429
EcoICRI GAGCTC 1 cut(s) 397
EcoO109I RGGNCCY 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 335
EcoT14I CCWWGG 2 cut(s) 83, 670
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 2 cut(s) 83, 670
FaeI CATG 5 cut(s) 87, 112, 243, 674, 803
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 1 cut(s) 627
FatI CATG 5 cut(s) 83, 108, 239, 670, 799
Fnu4HI GCNGC 3 cut(s) 74, 267, 734
FokI GGATG 4 cut(s) 110, 358, 397, 676
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 3 cut(s) 74, 267, 734
FspBI CTAG 2 cut(s) 213, 528
GlaI GCGC 2 cut(s) 77, 238
GluI GCNGC 3 cut(s) 74, 267, 734
GsuI CTGGAG 1 cut(s) 400
HhaI GCGC 2 cut(s) 78, 239
Hin1II CATG 5 cut(s) 87, 112, 243, 674, 803
Hin6I GCGC 2 cut(s) 76, 237
HinP1I GCGC 2 cut(s) 76, 237
HinfI GANTC 6 cut(s) 216, 305, 325, 469, 485, 506
HphI GGTGA 1 cut(s) 641
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 5 cut(s) 39, 147, 310, 330, 490
Hpy188III TCNNGA 1 cut(s) 19
Hpy8I GTNNAC 1 cut(s) 484
HpyAV CCTTC 7 cut(s) 16, 40, 142, 154, 198, 241, 803
HpyCH4III ACNGT 5 cut(s) 16, 258, 273, 371, 598
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 5 cut(s) 169, 261, 266, 438, 736
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 299
HpyF3I CTNAG 5 cut(s) 429, 471, 487, 521, 770
HpySE526I ACGT 1 cut(s) 447
Hsp92II CATG 5 cut(s) 87, 112, 243, 674, 803
HspAI GCGC 2 cut(s) 76, 237
Kzo9I GATC 5 cut(s) 34, 163, 351, 717, 773
LmnI GCTCC 3 cut(s) 255, 402, 763
Lsp1109I GCAGC 3 cut(s) 85, 278, 720
LweI GCATC 1 cut(s) 775
MaeI CTAG 2 cut(s) 213, 528
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 1 cut(s) 460
MalI GATC 5 cut(s) 36, 165, 353, 719, 775
MboI GATC 5 cut(s) 34, 163, 351, 717, 773
MboII GAAGA 7 cut(s) 97, 131, 361, 373, 553, 700, 744
MflI RGATCY 1 cut(s) 163
MhlI GDGCHC 1 cut(s) 399
MluCI AATT 3 cut(s) 170, 364, 535
MlyI GAGTC 5 cut(s) 210, 314, 463, 479, 515
MmeI TCCRAC 2 cut(s) 277, 430
MnlI CCTC 9 cut(s) 63, 182, 287, 375, 424, 496, 649, 722, 753
MseI TTAA 1 cut(s) 141
MspA1I CMGCKG 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 337
MvaI CCWGG 1 cut(s) 337
MwoI GCNNNNNNNGC 2 cut(s) 79, 299
NcoI CCATGG 2 cut(s) 83, 670
NdeII GATC 5 cut(s) 34, 163, 351, 717, 773
NlaIII CATG 5 cut(s) 87, 112, 243, 674, 803
NlaIV GGNNCC 4 cut(s) 89, 199, 426, 681
NspI RCATGY 1 cut(s) 243
NspV TTCGAA 1 cut(s) 117
PaqCI CACCTGC 1 cut(s) 691
PfeI GAWTC 1 cut(s) 325
PkrI GCNGC 3 cut(s) 75, 268, 735
PleI GAGTC 5 cut(s) 210, 313, 463, 479, 514
PpsI GAGTC 5 cut(s) 210, 313, 463, 479, 514
PpuMI RGGWCCY 1 cut(s) 49
PshAI GACNNNNGTC 1 cut(s) 47
Psp124BI GAGCTC 1 cut(s) 399
Psp5II RGGWCCY 1 cut(s) 49
Psp6I CCWGG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 335
PspN4I GGNNCC 4 cut(s) 89, 199, 426, 681
PspPI GGNCC 1 cut(s) 49
PspPPI RGGWCCY 1 cut(s) 49
PsuI RGATCY 1 cut(s) 163
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
SacI GAGCTC 1 cut(s) 399
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 3 cut(s) 74, 267, 734
Sau3AI GATC 5 cut(s) 34, 163, 351, 717, 773
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 5 cut(s) 210, 314, 463, 479, 515
ScrFI CCNGG 1 cut(s) 337
SduI GDGCHC 1 cut(s) 399
SfaNI GCATC 1 cut(s) 775
SfcI CTRYAG 1 cut(s) 607
SfuI TTCGAA 1 cut(s) 117
SinI GGWCC 1 cut(s) 49
Sse9I AATT 3 cut(s) 170, 364, 535
SspMI CTAG 2 cut(s) 213, 528
SstI GAGCTC 1 cut(s) 399
StyD4I CCNGG 1 cut(s) 335
StyI CCWWGG 2 cut(s) 83, 670
TaaI ACNGT 5 cut(s) 16, 258, 273, 371, 598
TaiI ACGT 1 cut(s) 450
TaqI TCGA 2 cut(s) 117, 467
TasI AATT 3 cut(s) 170, 364, 535
TatI WGTACW 1 cut(s) 474
TfiI GAWTC 1 cut(s) 325
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 3 cut(s) 19, 278, 591
TseI GCWGC 3 cut(s) 73, 266, 733
TspDTI ATGAA 4 cut(s) 97, 173, 362, 401
TspRI CASTG 3 cut(s) 19, 278, 591
VpaK11BI GGWCC 1 cut(s) 49
XceI RCATGY 1 cut(s) 243
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XspI CTAG 2 cut(s) 213, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.