Rmu_sc0007349.1_g000003

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007349.1
N/A
Physical Location & Seq
Forward (+)
12122 .. 13688
1567 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
atggtgccatttcaggtcattcgtagcaccttctggatggagcgccagaggttcccaagggatggtgagttaaagaatccttacaattcatcggtggaagtttgtggatcaagtagtggacctgcaatatcagtagctgctaatatggtttctgtgacattagggacggagactgacagctccattatctgtccggctagctttaactctgtggtgggcatcaaacccactgttggtctcactagtcgagcaggagtcataccaatcagtccaagacaggacacagttgggccgatttgtaggactgtagcggatgctgttcacgtccttgatatcattgttggcattgataggaatgataatgcaacaagtgaagcatcaaaggaggtgatagagttcaacaagaatcattcaaaggtggaaaagatcaatgaatatgggcaagatgtcttcgaattagctgagaagactaatgggatgggaccaaaagagcaggaggcattgtcaaatttagaaagactatctagacaggggttcaagaagttgatgaccgatcatagcctagatgccttggtggcttatgctaacagtgcttctagtatcctcgcaattggtggttttccgggcattgttgtgccggcaggatatcataacactaaaaagtatccttttgggatatgctttgggggactcaagggttcagagccaaagctgattgagatcgcctatgcctttgagcaagcaactaagatgaggagaaagcctcctctagcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

258

Amino Acids

27.88

Weight (kDa)

7.66

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 22 - 172 4.7e-36 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 62
AciI CCGC 1 cut(s) 311
AclWI GGATC 1 cut(s) 115
AcsI RAATTY 1 cut(s) 508
AfiI CCNNNNNNNGG 2 cut(s) 62, 233
AgsI TTSAA 3 cut(s) 400, 414, 538
AhlI ACTAGT 1 cut(s) 242
AjiI CACGTC 1 cut(s) 325
AluBI AGCT 5 cut(s) 137, 180, 201, 461, 712
AluI AGCT 5 cut(s) 137, 180, 201, 461, 712
Alw26I GTCTC 2 cut(s) 164, 242
AlwI GGATC 1 cut(s) 115
AlwNI CAGNNNCTG 1 cut(s) 137
AoxI GGCC 1 cut(s) 290
ApeKI GCWGC 1 cut(s) 137
ApoI RAATTY 1 cut(s) 508
AspLEI GCGC 1 cut(s) 45
AspS9I GGNCC 3 cut(s) 119, 290, 482
AsuC2I CCSGG 1 cut(s) 624
AsuHPI GGTGA 2 cut(s) 77, 400
AsuII TTCGAA 1 cut(s) 453
AsuNHI GCTAGC 1 cut(s) 197
AvaII GGWCC 2 cut(s) 119, 482
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 2 cut(s) 442, 473
BbvI GCAGC 1 cut(s) 124
BccI CCATC 3 cut(s) 31, 56, 472
BciVI GTATCC 2 cut(s) 611, 675
BcnI CCSGG 1 cut(s) 624
BcoDI GTCTC 2 cut(s) 164, 242
BcuI ACTAGT 1 cut(s) 242
BfaI CTAG 6 cut(s) 198, 243, 525, 563, 597, 770
BfmI CTRYAG 1 cut(s) 306
BfoI RGCGCY 1 cut(s) 46
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 2 cut(s) 611, 675
BglI GCCNNNNNGGC 1 cut(s) 575
BisI GCNGC 1 cut(s) 138
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 1 cut(s) 624
Bme18I GGWCC 2 cut(s) 119, 482
BmgBI CACGTC 1 cut(s) 325
BmgT120I GGNCC 3 cut(s) 119, 290, 482
BmiI GGNNCC 3 cut(s) 6, 53, 483
BmrFI CCNGG 1 cut(s) 624
BmsI GCATC 4 cut(s) 228, 304, 386, 556
BmtI GCTAGC 1 cut(s) 201
BpiI GAAGAC 2 cut(s) 442, 473
BplI GAGNNNNNCTC 1 cut(s) 748
Bpu14I TTCGAA 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 677
BpuMI CCSGG 1 cut(s) 624
BsaBI GATNNNNATC 1 cut(s) 719
BsaI GGTCTC 1 cut(s) 242
BsaJI CCNNGG 2 cut(s) 56, 570
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 233
Bse118I RCCGGY 1 cut(s) 637
Bse8I GATNNNNATC 1 cut(s) 719
BseDI CCNNGG 2 cut(s) 56, 570
BseGI GGATG 4 cut(s) 42, 67, 319, 483
BseJI GATNNNNATC 1 cut(s) 719
BseLI CCNNNNNNNGG 2 cut(s) 62, 233
BseMII CTCAG 1 cut(s) 453
BseRI GAGGAG 2 cut(s) 756, 769
BseXI GCAGC 1 cut(s) 124
BshFI GGCC 1 cut(s) 292
BshNI GGYRCC 1 cut(s) 4
BsiSI CCGG 3 cut(s) 194, 623, 638
BslFI GGGAC 3 cut(s) 178, 495, 702
BslI CCNNNNNNNGG 2 cut(s) 62, 233
BsmAI GTCTC 2 cut(s) 164, 242
BsmFI GGGAC 3 cut(s) 178, 495, 702
BsnI GGCC 1 cut(s) 292
Bso31I GGTCTC 1 cut(s) 242
Bsp119I TTCGAA 1 cut(s) 453
Bsp143I GATC 4 cut(s) 107, 426, 553, 720
BspACI CCGC 1 cut(s) 311
BspANI GGCC 1 cut(s) 292
BspCNI CTCAG 1 cut(s) 454
BspLI GGNNCC 3 cut(s) 6, 53, 483
BspMI ACCTGC 1 cut(s) 130
BspOI GCTAGC 1 cut(s) 201
BspPI GGATC 1 cut(s) 115
BspT104I TTCGAA 1 cut(s) 453
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 242
BsrFI RCCGGY 1 cut(s) 637
BssAI RCCGGY 1 cut(s) 637
BssECI CCNNGG 2 cut(s) 56, 570
BssMI GATC 4 cut(s) 107, 426, 553, 720
BssT1I CCWWGG 2 cut(s) 56, 570
Bst4CI ACNGT 4 cut(s) 232, 286, 307, 590
BstBI TTCGAA 1 cut(s) 453
BstC8I GCNNGC 3 cut(s) 199, 639, 741
BstDEI CTNAG 2 cut(s) 462, 747
BstF5I GGATG 4 cut(s) 42, 67, 319, 483
BstH2I RGCGCY 1 cut(s) 46
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 4 cut(s) 110, 429, 556, 723
BstMAI GTCTC 2 cut(s) 164, 242
BstMBI GATC 4 cut(s) 107, 426, 553, 720
BstMWI GCNNNNNNNGC 2 cut(s) 575, 590
BstSCI CCNGG 1 cut(s) 622
BstSFI CTRYAG 1 cut(s) 306
BstV1I GCAGC 1 cut(s) 124
BstV2I GAAGAC 2 cut(s) 442, 473
BsuI GTATCC 2 cut(s) 611, 675
BsuRI GGCC 1 cut(s) 292
BtrI CACGTC 1 cut(s) 325
BtsCI GGATG 4 cut(s) 42, 67, 319, 483
BtsIMutI CAGTG 2 cut(s) 228, 595
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 3 cut(s) 199, 639, 741
CaiI CAGNNNCTG 1 cut(s) 137
CfoI GCGC 1 cut(s) 45
Cfr10I RCCGGY 1 cut(s) 637
Cfr13I GGNCC 3 cut(s) 119, 290, 482
DdeI CTNAG 2 cut(s) 462, 747
DpnI GATC 4 cut(s) 109, 428, 555, 722
DpnII GATC 4 cut(s) 107, 426, 553, 720
Eco130I CCWWGG 2 cut(s) 56, 570
Eco31I GGTCTC 1 cut(s) 242
Eco32I GATATC 2 cut(s) 334, 647
Eco47I GGWCC 2 cut(s) 119, 482
EcoRV GATATC 2 cut(s) 334, 647
EcoT14I CCWWGG 2 cut(s) 56, 570
ErhI CCWWGG 2 cut(s) 56, 570
FaiI YATR 8 cut(s) 146, 260, 438, 558, 582, 651, 679, 729
FaqI GGGAC 3 cut(s) 178, 495, 702
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 4 cut(s) 49, 74, 326, 490
Fsp4HI GCNGC 1 cut(s) 138
FspBI CTAG 6 cut(s) 198, 243, 525, 563, 597, 770
GlaI GCGC 1 cut(s) 44
GluI GCNGC 1 cut(s) 138
HaeII RGCGCY 1 cut(s) 46
HaeIII GGCC 1 cut(s) 292
HapII CCGG 3 cut(s) 194, 623, 638
HhaI GCGC 1 cut(s) 45
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HinfI GANTC 4 cut(s) 76, 255, 406, 690
HpaII CCGG 3 cut(s) 194, 623, 638
HphI GGTGA 2 cut(s) 77, 400
Hpy166II GTNNAC 2 cut(s) 119, 322
Hpy188I TCNGA 1 cut(s) 703
Hpy188III TCNNGA 3 cut(s) 34, 525, 538
Hpy8I GTNNAC 2 cut(s) 119, 322
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 4 cut(s) 232, 286, 307, 590
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 2 cut(s) 125, 365
HpyF10VI GCNNNNNNNGC 2 cut(s) 575, 590
HpyF3I CTNAG 2 cut(s) 462, 747
HpySE526I ACGT 1 cut(s) 324
HspAI GCGC 1 cut(s) 43
KroI GCCGGC 1 cut(s) 637
KroNI GCCGGC 1 cut(s) 639
Kzo9I GATC 4 cut(s) 107, 426, 553, 720
LmnI GCTCC 2 cut(s) 40, 185
Lsp1109I GCAGC 1 cut(s) 124
LweI GCATC 4 cut(s) 228, 304, 386, 556
MaeI CTAG 6 cut(s) 198, 243, 525, 563, 597, 770
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 1 cut(s) 154
MalI GATC 4 cut(s) 109, 428, 555, 722
MboI GATC 4 cut(s) 107, 426, 553, 720
MboII GAAGA 2 cut(s) 442, 478
MfeI CAATTG 1 cut(s) 609
MluCI AATT 4 cut(s) 85, 455, 508, 609
MlyI GAGTC 2 cut(s) 264, 684
MnlI CCTC 7 cut(s) 42, 379, 490, 614, 747, 774, 777
MroNI GCCGGC 1 cut(s) 637
MseI TTAA 2 cut(s) 71, 204
MslI CAYNNNNRTG 1 cut(s) 632
MspI CCGG 3 cut(s) 194, 623, 638
MspR9I CCNGG 1 cut(s) 624
MunI CAATTG 1 cut(s) 609
MwoI GCNNNNNNNGC 2 cut(s) 575, 590
NaeI GCCGGC 1 cut(s) 639
NciI CCSGG 1 cut(s) 624
NdeII GATC 4 cut(s) 107, 426, 553, 720
NgoMIV GCCGGC 1 cut(s) 637
NheI GCTAGC 1 cut(s) 197
NlaIV GGNNCC 3 cut(s) 6, 53, 483
NmuCI GTSAC 1 cut(s) 154
NspV TTCGAA 1 cut(s) 453
PdiI GCCGGC 1 cut(s) 639
PfeI GAWTC 2 cut(s) 76, 406
PflMI CCANNNNNTGG 1 cut(s) 62
PkrI GCNGC 1 cut(s) 139
PleI GAGTC 2 cut(s) 263, 684
PpsI GAGTC 2 cut(s) 263, 684
PspN4I GGNNCC 3 cut(s) 6, 53, 483
PspPI GGNCC 3 cut(s) 119, 290, 482
PstNI CAGNNNCTG 1 cut(s) 137
RseI CAYNNNNRTG 1 cut(s) 632
SaqAI TTAA 2 cut(s) 71, 204
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 4 cut(s) 107, 426, 553, 720
Sau96I GGNCC 3 cut(s) 119, 290, 482
SchI GAGTC 2 cut(s) 264, 684
ScrFI CCNGG 1 cut(s) 624
SfaNI GCATC 4 cut(s) 228, 304, 386, 556
SfcI CTRYAG 1 cut(s) 306
SfuI TTCGAA 1 cut(s) 453
SinI GGWCC 2 cut(s) 119, 482
SmiMI CAYNNNNRTG 1 cut(s) 632
SmlI CTYRAG 1 cut(s) 692
SmoI CTYRAG 1 cut(s) 692
SpeI ACTAGT 1 cut(s) 242
Sse9I AATT 4 cut(s) 85, 455, 508, 609
SsiI CCGC 1 cut(s) 311
SspMI CTAG 6 cut(s) 198, 243, 525, 563, 597, 770
StyD4I CCNGG 1 cut(s) 622
StyI CCWWGG 2 cut(s) 56, 570
TaaI ACNGT 4 cut(s) 232, 286, 307, 590
TaiI ACGT 1 cut(s) 327
TaqI TCGA 2 cut(s) 247, 453
TaqII GACCGA 1 cut(s) 566
TasI AATT 4 cut(s) 85, 455, 508, 609
TfiI GAWTC 2 cut(s) 76, 406
Tru1I TTAA 2 cut(s) 71, 204
Tru9I TTAA 2 cut(s) 71, 204
TscAI CASTG 2 cut(s) 235, 595
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 1 cut(s) 137
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 2 cut(s) 78, 447
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 2 cut(s) 235, 595
Van91I CCANNNNNTGG 1 cut(s) 62
VpaK11BI GGWCC 2 cut(s) 119, 482
XapI RAATTY 1 cut(s) 508
XbaI TCTAGA 1 cut(s) 524
XspI CTAG 6 cut(s) 198, 243, 525, 563, 597, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.