Rmu_sc0010754.1_g000016

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010754.1
N/A
Physical Location & Seq
Forward (+)
49582 .. 51339
1758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010754.1_g000016.1.cds

Sequence Viewer

Length: 513 bp
atggattactgtgaagaagaaagggaagacatggagttgccgctatttgacttcaccactattgctgatgccactgataacttttcaagcaacaacaaactgggactaggcggttttggacctgtgtacaagatcgtcttcttcttggacttcgacatcatcagaccagccgcctctatctcctgtcccgatcagcttcatcttcctcgttcaaacccaacgttttgcttgggcactagaacttctccagatcagtttccgtccagccagttgctccgtttggtgacgtcatcatcagatcggacccgtccaaacaaacccggcccgcgacttcctcaacccagtcttgcacaccccatcgttgagcttctcagccttgtcgcgagcatccagtcgtcgatccgtcatcaagcttctcagccccgtcgcactcatcccgtcgtcgactcagctcagacccttaccgttgtgcgctcagactcactgattcatccacattatgctttacggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

170

Amino Acids

18.94

Weight (kDa)

6.28

Isoelectric Point (pI)

61.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 290
AccI GTMKAC 1 cut(s) 444
AccII CGCG 2 cut(s) 328, 383
AciI CCGC 4 cut(s) 41, 111, 171, 326
AclI AACGTT 1 cut(s) 221
AclWI GGATC 1 cut(s) 394
AcyI GRCGYC 1 cut(s) 287
AfaI GTAC 1 cut(s) 128
AgsI TTSAA 2 cut(s) 87, 213
AluBI AGCT 4 cut(s) 196, 367, 413, 452
AluI AGCT 4 cut(s) 196, 367, 413, 452
AlwI GGATC 1 cut(s) 394
AoxI GGCC 1 cut(s) 322
AspLEI GCGC 1 cut(s) 474
AspS9I GGNCC 3 cut(s) 119, 303, 323
AsuC2I CCSGG 1 cut(s) 321
AsuHPI GGTGA 2 cut(s) 46, 295
AvaII GGWCC 2 cut(s) 119, 303
BaeGI GKGCMC 1 cut(s) 236
BbsI GAAGAC 2 cut(s) 33, 130
BccI CCATC 1 cut(s) 365
BcnI CCSGG 1 cut(s) 321
BfaI CTAG 2 cut(s) 107, 237
BisI GCNGC 2 cut(s) 41, 171
BlsI GCNGC 2 cut(s) 42, 172
Bme1390I CCNGG 1 cut(s) 321
Bme18I GGWCC 2 cut(s) 119, 303
BmgT120I GGNCC 3 cut(s) 119, 303, 323
BmiI GGNNCC 1 cut(s) 305
BmrFI CCNGG 1 cut(s) 321
BmrI ACTGGG 2 cut(s) 110, 336
BmsI GCATC 2 cut(s) 58, 396
BmuI ACTGGG 2 cut(s) 110, 336
BpiI GAAGAC 2 cut(s) 33, 130
BpmI CTGGAG 1 cut(s) 231
BpuMI CCSGG 1 cut(s) 321
BsaHI GRCGYC 1 cut(s) 287
Bse1I ACTGG 4 cut(s) 105, 268, 342, 391
BseGI GGATG 3 cut(s) 387, 433, 490
BseMII CTCAG 5 cut(s) 385, 431, 462, 467, 489
BseNI ACTGG 4 cut(s) 105, 268, 342, 391
BseSI GKGCMC 1 cut(s) 236
Bsh1236I CGCG 2 cut(s) 328, 383
BshFI GGCC 1 cut(s) 324
BsiSI CCGG 1 cut(s) 321
BslFI GGGAC 2 cut(s) 117, 171
BsmFI GGGAC 2 cut(s) 117, 171
BsnI GGCC 1 cut(s) 324
Bsp1286I GDGCHC 1 cut(s) 236
Bsp1407I TGTACA 1 cut(s) 126
Bsp143I GATC 5 cut(s) 132, 190, 250, 298, 399
Bsp68I TCGCGA 1 cut(s) 383
BspACI CCGC 4 cut(s) 41, 111, 171, 326
BspANI GGCC 1 cut(s) 324
BspCNI CTCAG 5 cut(s) 384, 430, 461, 466, 488
BspFNI CGCG 2 cut(s) 328, 383
BspLI GGNNCC 1 cut(s) 305
BspPI GGATC 1 cut(s) 394
BsrGI TGTACA 1 cut(s) 126
BsrI ACTGG 4 cut(s) 105, 268, 342, 391
BssMI GATC 5 cut(s) 132, 190, 250, 298, 399
BssNI GRCGYC 1 cut(s) 287
Bst4CI ACNGT 3 cut(s) 11, 466, 510
BstACI GRCGYC 1 cut(s) 287
BstAUI TGTACA 1 cut(s) 126
BstC8I GCNNGC 2 cut(s) 326, 385
BstDEI CTNAG 5 cut(s) 371, 417, 448, 453, 475
BstF5I GGATG 3 cut(s) 387, 433, 490
BstFNI CGCG 2 cut(s) 328, 383
BstHHI GCGC 1 cut(s) 474
BstKTI GATC 5 cut(s) 135, 193, 253, 301, 402
BstMBI GATC 5 cut(s) 132, 190, 250, 298, 399
BstSCI CCNGG 1 cut(s) 319
BstSLI GKGCMC 1 cut(s) 236
BstUI CGCG 2 cut(s) 328, 383
BstV2I GAAGAC 2 cut(s) 33, 130
BsuRI GGCC 1 cut(s) 324
BtsCI GGATG 3 cut(s) 387, 433, 490
BtsIMutI CAGTG 2 cut(s) 72, 482
BtuMI TCGCGA 1 cut(s) 383
Cac8I GCNNGC 2 cut(s) 326, 385
CfoI GCGC 1 cut(s) 474
Cfr13I GGNCC 3 cut(s) 119, 303, 323
Csp6I GTAC 1 cut(s) 127
CviAII CATG 1 cut(s) 31
CviJI RGCY 9 cut(s) 170, 196, 267, 324, 367, 375, 413, 421, 452
CviKI_1 RGCY 9 cut(s) 170, 196, 267, 324, 367, 375, 413, 421, 452
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 5 cut(s) 371, 417, 448, 453, 475
DpnI GATC 5 cut(s) 134, 192, 252, 300, 401
DpnII GATC 5 cut(s) 132, 190, 250, 298, 399
Eco47I GGWCC 2 cut(s) 119, 303
FaeI CATG 1 cut(s) 34
FaiI YATR 2 cut(s) 32, 501
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FaqI GGGAC 2 cut(s) 117, 171
FatI CATG 1 cut(s) 30
FauI CCCGC 1 cut(s) 333
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 2 cut(s) 41, 171
FokI GGATG 3 cut(s) 374, 420, 477
Fsp4HI GCNGC 2 cut(s) 41, 171
FspBI CTAG 2 cut(s) 107, 237
GlaI GCGC 1 cut(s) 473
GluI GCNGC 2 cut(s) 41, 171
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 1 cut(s) 324
HapII CCGG 1 cut(s) 321
HhaI GCGC 1 cut(s) 474
Hin1I GRCGYC 1 cut(s) 287
Hin1II CATG 1 cut(s) 34
Hin6I GCGC 1 cut(s) 472
HinP1I GCGC 1 cut(s) 472
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 3 cut(s) 446, 479, 487
HpaII CCGG 1 cut(s) 321
HphI GGTGA 2 cut(s) 46, 295
Hpy166II GTNNAC 2 cut(s) 127, 445
Hpy188I TCNGA 5 cut(s) 164, 298, 303, 456, 478
Hpy188III TCNNGA 3 cut(s) 188, 248, 382
Hpy8I GTNNAC 2 cut(s) 127, 445
Hpy99I CGWCG 4 cut(s) 400, 429, 443, 446
HpyCH4III ACNGT 3 cut(s) 11, 466, 510
HpyCH4IV ACGT 2 cut(s) 221, 287
HpyCH4V TGCA 1 cut(s) 350
HpyF3I CTNAG 5 cut(s) 371, 417, 448, 453, 475
HpySE526I ACGT 2 cut(s) 221, 287
Hsp92I GRCGYC 1 cut(s) 287
Hsp92II CATG 1 cut(s) 34
HspAI GCGC 1 cut(s) 472
Kzo9I GATC 5 cut(s) 132, 190, 250, 298, 399
LmnI GCTCC 1 cut(s) 279
LweI GCATC 2 cut(s) 58, 396
MaeI CTAG 2 cut(s) 107, 237
MaeII ACGT 2 cut(s) 221, 287
MaeIII GTNAC 1 cut(s) 283
MalI GATC 5 cut(s) 134, 192, 252, 300, 401
MboI GATC 5 cut(s) 132, 190, 250, 298, 399
MboII GAAGA 6 cut(s) 26, 29, 38, 130, 133, 194
MhlI GDGCHC 1 cut(s) 236
MlyI GAGTC 2 cut(s) 440, 473
MnlI CCTC 3 cut(s) 184, 216, 345
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 1 cut(s) 321
MvnI CGCG 2 cut(s) 328, 383
NciI CCSGG 1 cut(s) 321
NdeII GATC 5 cut(s) 132, 190, 250, 298, 399
NlaIII CATG 1 cut(s) 34
NlaIV GGNNCC 1 cut(s) 305
NmuCI GTSAC 1 cut(s) 283
NruI TCGCGA 1 cut(s) 383
PfeI GAWTC 1 cut(s) 487
PkrI GCNGC 2 cut(s) 42, 172
PleI GAGTC 2 cut(s) 440, 473
PpsI GAGTC 2 cut(s) 440, 473
Psp1406I AACGTT 1 cut(s) 221
PspN4I GGNNCC 1 cut(s) 305
PspPI GGNCC 3 cut(s) 119, 303, 323
RruI TCGCGA 1 cut(s) 383
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
SalI GTCGAC 1 cut(s) 443
SatI GCNGC 2 cut(s) 41, 171
Sau3AI GATC 5 cut(s) 132, 190, 250, 298, 399
Sau96I GGNCC 3 cut(s) 119, 303, 323
SchI GAGTC 2 cut(s) 440, 473
ScrFI CCNGG 1 cut(s) 321
SduI GDGCHC 1 cut(s) 236
SetI ASST 7 cut(s) 124, 198, 224, 290, 369, 415, 454
SfaNI GCATC 2 cut(s) 58, 396
SinI GGWCC 2 cut(s) 119, 303
SsiI CCGC 4 cut(s) 41, 111, 171, 326
SspMI CTAG 2 cut(s) 107, 237
StyD4I CCNGG 1 cut(s) 319
TaaI ACNGT 3 cut(s) 11, 466, 510
TaiI ACGT 2 cut(s) 224, 290
TaqI TCGA 3 cut(s) 153, 398, 444
TatI WGTACW 1 cut(s) 126
TauI GCSGC 2 cut(s) 43, 173
TfiI GAWTC 1 cut(s) 487
TscAI CASTG 2 cut(s) 79, 489
TseFI GTSAC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 2 cut(s) 188, 479
TspGWI ACGGA 3 cut(s) 249, 266, 392
TspRI CASTG 2 cut(s) 79, 489
VpaK11BI GGWCC 2 cut(s) 119, 303
XmiI GTMKAC 1 cut(s) 444
XspI CTAG 2 cut(s) 107, 237
ZraI GACGTC 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.