Rmu_sc0016540.1_g000001
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0016540.1
N/A
Physical Location & Seq
Reverse (-)
2 .. 693
692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0016540.1_g000001.1.cds

Sequence Viewer

Length: 519 bp
atggctggaatagaaggtcatgcaattggaattgatctcggcacaaagtactcatgtgtgggagtttggaagcatgataacaataatgttgatatcatagtgaacgatcagggcaacaggaccactccgtcatgtgttgccttcactgatactgagcttttggtaggagaagcagcgtttaatcaggtcgtaagaaaccctgacaactccatcttcgatgcaaagcggttaattggcaggaaattcagtgatgcatctgttcaaagtgacaagaagctctggccattcaaggtggttgaaggtcctgatgataagcctatgattttggttatgcatgaaggcaaagagaaacagtgtgctgctgaagagatctcaagtatggttcttgcaaaaatgcgggagattgctgaagtctatcttggatccactgtgaagaatgctgtgatcacggttcctgcttactttaataattcacagcgtgaggctacaaaaaaagctggtattactgcaggcctaaat
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

173

Amino Acids

18.87

Weight (kDa)

5.93

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 127
AciI CCGC 2 cut(s) 226, 397
AclWI GGATC 2 cut(s) 417, 430
AcoI YGGCCR 1 cut(s) 281
AcsI RAATTY 1 cut(s) 242
AcuI CTGAAG 2 cut(s) 384, 429
AdeI CACNNNGTG 1 cut(s) 479
AfaI GTAC 1 cut(s) 50
AgsI TTSAA 3 cut(s) 263, 289, 299
AjuI GAANNNNNNNTTGG 2 cut(s) 402, 434
AluBI AGCT 3 cut(s) 157, 277, 497
AluI AGCT 3 cut(s) 157, 277, 497
AlwI GGATC 2 cut(s) 417, 430
AoxI GGCC 2 cut(s) 281, 511
ApeKI GCWGC 2 cut(s) 173, 359
ApoI RAATTY 1 cut(s) 242
AspS9I GGNCC 2 cut(s) 120, 302
AvaII GGWCC 2 cut(s) 120, 302
BalI TGGCCA 1 cut(s) 283
BamHI GGATCC 1 cut(s) 422
BbvI GCAGC 2 cut(s) 185, 346
BccI CCATC 1 cut(s) 218
BclI TGATCA 1 cut(s) 444
BfmI CTRYAG 1 cut(s) 507
BglII AGATCT 1 cut(s) 369
BisI GCNGC 2 cut(s) 174, 360
BlsI GCNGC 2 cut(s) 175, 361
BmcAI AGTACT 1 cut(s) 50
Bme18I GGWCC 2 cut(s) 120, 302
BmgT120I GGNCC 2 cut(s) 120, 302
BmiI GGNNCC 2 cut(s) 424, 453
BmsI GCATC 3 cut(s) 208, 241, 263
BpuEI CTTGAG 1 cut(s) 358
BseMII CTCAG 1 cut(s) 144
BseXI GCAGC 2 cut(s) 185, 346
BshFI GGCC 2 cut(s) 283, 513
BsmI GAATGC 1 cut(s) 442
BsnI GGCC 2 cut(s) 283, 513
Bsp143I GATC 5 cut(s) 34, 106, 369, 422, 444
BspACI CCGC 2 cut(s) 226, 397
BspANI GGCC 2 cut(s) 283, 513
BspCNI CTCAG 1 cut(s) 145
BspLI GGNNCC 2 cut(s) 424, 453
BspMAI CTGCAG 1 cut(s) 511
BspPI GGATC 2 cut(s) 417, 430
BssMI GATC 5 cut(s) 34, 106, 369, 422, 444
Bst4CI ACNGT 3 cut(s) 354, 430, 451
Bst6I CTCTTC 1 cut(s) 360
BstC8I GCNNGC 1 cut(s) 511
BstDEI CTNAG 1 cut(s) 153
BstKTI GATC 5 cut(s) 37, 109, 372, 425, 447
BstMBI GATC 5 cut(s) 34, 106, 369, 422, 444
BstSFI CTRYAG 1 cut(s) 507
BstV1I GCAGC 2 cut(s) 185, 346
BstX2I RGATCY 2 cut(s) 369, 422
BstYI RGATCY 2 cut(s) 369, 422
BsuRI GGCC 2 cut(s) 283, 513
BtsIMutI CAGTG 4 cut(s) 144, 253, 359, 426
Cac8I GCNNGC 1 cut(s) 511
Cfr13I GGNCC 2 cut(s) 120, 302
Csp6I GTAC 1 cut(s) 49
CviAII CATG 5 cut(s) 20, 54, 74, 132, 335
CviJI RGCY 8 cut(s) 5, 157, 277, 283, 316, 485, 497, 513
CviKI_1 RGCY 8 cut(s) 5, 157, 277, 283, 316, 485, 497, 513
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 1 cut(s) 153
DpnI GATC 5 cut(s) 36, 108, 371, 424, 446
DpnII GATC 5 cut(s) 34, 106, 369, 422, 444
DraIII CACNNNGTG 1 cut(s) 479
DrdI GACNNNNNNGTC 1 cut(s) 127
DseDI GACNNNNNNGTC 1 cut(s) 127
EaeI YGGCCR 1 cut(s) 281
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
Eco147I AGGCCT 1 cut(s) 513
Eco32I GATATC 1 cut(s) 94
Eco47I GGWCC 2 cut(s) 120, 302
Eco57I CTGAAG 2 cut(s) 384, 429
EcoO109I RGGNCCY 1 cut(s) 302
EcoRV GATATC 1 cut(s) 94
EcoT22I ATGCAT 2 cut(s) 256, 336
FaeI CATG 5 cut(s) 23, 57, 77, 135, 338
FaiI YATR 9 cut(s) 21, 55, 75, 98, 133, 320, 332, 336, 380
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FatI CATG 5 cut(s) 19, 53, 73, 131, 334
FauI CCCGC 1 cut(s) 390
FbaI TGATCA 1 cut(s) 444
Fnu4HI GCNGC 2 cut(s) 174, 360
Fsp4HI GCNGC 2 cut(s) 174, 360
GluI GCNGC 2 cut(s) 174, 360
HaeIII GGCC 2 cut(s) 283, 513
Hin1II CATG 5 cut(s) 23, 57, 77, 135, 338
Hpy166II GTNNAC 1 cut(s) 103
Hpy188III TCNNGA 1 cut(s) 305
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 4 cut(s) 8, 151, 293, 332
HpyCH4III ACNGT 3 cut(s) 354, 430, 451
HpyCH4V TGCA 6 cut(s) 23, 221, 254, 334, 389, 509
HpyF3I CTNAG 1 cut(s) 153
Hsp92II CATG 5 cut(s) 23, 57, 77, 135, 338
Ksp22I TGATCA 1 cut(s) 444
Kzo9I GATC 5 cut(s) 34, 106, 369, 422, 444
Lsp1109I GCAGC 2 cut(s) 185, 346
LweI GCATC 3 cut(s) 208, 241, 263
MaeIII GTNAC 1 cut(s) 266
MalI GATC 5 cut(s) 36, 108, 371, 424, 446
MboI GATC 5 cut(s) 34, 106, 369, 422, 444
MboII GAAGA 3 cut(s) 205, 377, 445
MfeI CAATTG 1 cut(s) 24
MflI RGATCY 2 cut(s) 369, 422
MlsI TGGCCA 1 cut(s) 283
MluCI AATT 5 cut(s) 24, 30, 231, 242, 469
MluNI TGGCCA 1 cut(s) 283
MnlI CCTC 1 cut(s) 475
Mox20I TGGCCA 1 cut(s) 283
Mph1103I ATGCAT 2 cut(s) 256, 336
MscI TGGCCA 1 cut(s) 283
MseI TTAA 3 cut(s) 180, 230, 465
Msp20I TGGCCA 1 cut(s) 283
MunI CAATTG 1 cut(s) 24
Mva1269I GAATGC 1 cut(s) 442
NdeII GATC 5 cut(s) 34, 106, 369, 422, 444
NlaIII CATG 5 cut(s) 23, 57, 77, 135, 338
NlaIV GGNNCC 2 cut(s) 424, 453
NmeAIII GCCGAG 1 cut(s) 18
NmuCI GTSAC 1 cut(s) 266
NsiI ATGCAT 2 cut(s) 256, 336
PceI AGGCCT 1 cut(s) 513
PctI GAATGC 1 cut(s) 442
PkrI GCNGC 2 cut(s) 175, 361
PpuMI RGGWCCY 1 cut(s) 302
Psp5II RGGWCCY 1 cut(s) 302
PspN4I GGNNCC 2 cut(s) 424, 453
PspPI GGNCC 2 cut(s) 120, 302
PspPPI RGGWCCY 1 cut(s) 302
PstI CTGCAG 1 cut(s) 511
PsuI RGATCY 2 cut(s) 369, 422
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SaqAI TTAA 3 cut(s) 180, 230, 465
SatI GCNGC 2 cut(s) 174, 360
Sau3AI GATC 5 cut(s) 34, 106, 369, 422, 444
Sau96I GGNCC 2 cut(s) 120, 302
ScaI AGTACT 1 cut(s) 50
SetI ASST 7 cut(s) 19, 159, 189, 279, 294, 304, 499
SfaNI GCATC 3 cut(s) 208, 241, 263
SfcI CTRYAG 1 cut(s) 507
SinI GGWCC 2 cut(s) 120, 302
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 5 cut(s) 24, 30, 231, 242, 469
SseBI AGGCCT 1 cut(s) 513
SsiI CCGC 2 cut(s) 226, 397
StuI AGGCCT 1 cut(s) 513
TaaI ACNGT 3 cut(s) 354, 430, 451
TaqI TCGA 1 cut(s) 216
TasI AATT 5 cut(s) 24, 30, 231, 242, 469
TatI WGTACW 1 cut(s) 48
Tru1I TTAA 3 cut(s) 180, 230, 465
Tru9I TTAA 3 cut(s) 180, 230, 465
TscAI CASTG 4 cut(s) 151, 253, 359, 433
TseFI GTSAC 1 cut(s) 266
TseI GCWGC 2 cut(s) 173, 359
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 1 cut(s) 351
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 4 cut(s) 151, 253, 359, 433
VpaK11BI GGWCC 2 cut(s) 120, 302
XapI RAATTY 1 cut(s) 242
ZrmI AGTACT 1 cut(s) 50
Zsp2I ATGCAT 2 cut(s) 256, 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.