Rmu_sc0030050.1_g000001
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0030050.1
N/A
Physical Location & Seq
Forward (+)
1 .. 872
872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0030050.1_g000001.1.cds

Sequence Viewer

Length: 694 bp
ggtcattgaaggacctgatgacaaaccaatgattatgtttactcacaaaggtcaagagaaacaaattgctcctgaagaagtctcatctatggttcttggcaagatgcgggatattgctgagtcattcctcggctcaactgtgaaaaatgcagtggtcactgtccctgatcacttcagtgacgcgcaacgtcaaggtacaaagagagctggtgctgctgctggcctaaatgtcatacgtattattaatgaaccaactgccgcagccattgcttatggaattgacaagaaggcaggatggccactgctggagacactcaccttggaggcgaagactttgataacagaatttaagaggaagcatgatttggacgttagtggaaactcgagagctcttaggaggttaagacatgcatgtgagaaggcaaagaggagactttcttatgcatttgacactgacattgaaattgactgtttggatcagggtattgatttctatacaacttttactcgtgccaaatttgaacaactgaacatggatttcttcaacaagtgtatggagcctgtggagaagtgtttaagggatgctaacatggatgtaaggagtgtcaatgatgttgatctttctgggggctcttctagaattcccaaggtacaacaactattgcagaatgtgttcaatggaaaggagttgtga

Protein Analysis

230

Amino Acids

25.72

Weight (kDa)

7.06

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 183
AciI CCGC 2 cut(s) 107, 259
AclWI GGATC 1 cut(s) 484
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 3 cut(s) 345, 516, 640
AcuI CTGAAG 2 cut(s) 94, 158
AfaI GTAC 2 cut(s) 197, 652
AgsI TTSAA 5 cut(s) 9, 462, 522, 545, 677
AjuI GAANNNNNNNTTGG 2 cut(s) 348, 380
AleI CACNNNNGTG 1 cut(s) 175
AluBI AGCT 2 cut(s) 207, 390
AluI AGCT 2 cut(s) 207, 390
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 3 cut(s) 86, 303, 425
AlwI GGATC 1 cut(s) 484
Ama87I CYCGRG 1 cut(s) 383
AoxI GGCC 2 cut(s) 221, 297
ApeKI GCWGC 3 cut(s) 213, 216, 261
ApoI RAATTY 3 cut(s) 345, 516, 640
AseI ATTAAT 1 cut(s) 244
Asp700I GAANNNNTTC 1 cut(s) 672
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 1 cut(s) 12
AsuHPI GGTGA 1 cut(s) 308
AvaI CYCGRG 1 cut(s) 383
AvaII GGWCC 1 cut(s) 12
BalI TGGCCA 1 cut(s) 299
BanII GRGCYC 2 cut(s) 392, 633
BauI CACGAG 1 cut(s) 508
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 392
BbvI GCAGC 3 cut(s) 200, 203, 273
BccI CCATC 1 cut(s) 289
BclI TGATCA 1 cut(s) 167
BcoDI GTCTC 3 cut(s) 86, 303, 425
BfaI CTAG 1 cut(s) 637
BisI GCNGC 4 cut(s) 214, 217, 259, 262
BlsI GCNGC 4 cut(s) 215, 218, 260, 263
Bme18I GGWCC 1 cut(s) 12
BmeT110I CYCGRG 1 cut(s) 383
BmgT120I GGNCC 1 cut(s) 12
BmiI GGNNCC 1 cut(s) 559
BmsI GCATC 2 cut(s) 94, 572
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 1 cut(s) 327
BsaAI YACGTR 1 cut(s) 237
BsaBI GATNNNNATC 1 cut(s) 616
BsaJI CCNNGG 3 cut(s) 128, 319, 646
Bse3DI GCAATG 1 cut(s) 265
Bse8I GATNNNNATC 1 cut(s) 616
BseDI CCNNGG 3 cut(s) 128, 319, 646
BseGI GGATG 3 cut(s) 300, 587, 599
BseJI GATNNNNATC 1 cut(s) 616
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 1 cut(s) 109
BseRI GAGGAG 1 cut(s) 443
BseXI GCAGC 3 cut(s) 200, 203, 273
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 2 cut(s) 223, 299
BsiHKAI GWGCWC 1 cut(s) 392
BsiHKCI CYCGRG 1 cut(s) 383
BslFI GGGAC 1 cut(s) 147
BsmAI GTCTC 3 cut(s) 86, 303, 425
BsmFI GGGAC 1 cut(s) 147
BsnI GGCC 2 cut(s) 223, 299
BsoBI CYCGRG 1 cut(s) 383
Bsp1286I GDGCHC 2 cut(s) 392, 633
Bsp143I GATC 3 cut(s) 167, 476, 617
BspACI CCGC 2 cut(s) 107, 259
BspANI GGCC 2 cut(s) 223, 299
BspCNI CTCAG 1 cut(s) 110
BspFNI CGCG 1 cut(s) 183
BspLI GGNNCC 1 cut(s) 559
BspPI GGATC 1 cut(s) 484
BspQI GCTCTTC 1 cut(s) 638
BsrDI GCAATG 1 cut(s) 265
BssECI CCNNGG 3 cut(s) 128, 319, 646
BssMI GATC 3 cut(s) 167, 476, 617
BssSI CACGAG 1 cut(s) 508
BssT1I CCWWGG 2 cut(s) 319, 646
Bst2BI CACGAG 1 cut(s) 508
Bst4CI ACNGT 3 cut(s) 140, 161, 471
Bst6I CTCTTC 1 cut(s) 638
BstAPI GCANNNNNTGC 1 cut(s) 267
BstBAI YACGTR 1 cut(s) 237
BstC8I GCNNGC 1 cut(s) 221
BstDEI CTNAG 2 cut(s) 118, 393
BstF5I GGATG 3 cut(s) 300, 587, 599
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 3 cut(s) 170, 479, 620
BstMAI GTCTC 3 cut(s) 86, 303, 425
BstMBI GATC 3 cut(s) 167, 476, 617
BstMWI GCNNNNNNNGC 2 cut(s) 213, 267
BstNSI RCATGY 2 cut(s) 411, 415
BstSNI TACGTA 1 cut(s) 237
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 3 cut(s) 200, 203, 273
BstV2I GAAGAC 1 cut(s) 336
BsuRI GGCC 2 cut(s) 223, 299
BtsCI GGATG 3 cut(s) 300, 587, 599
BtsI GCAGTG 2 cut(s) 157, 300
BtsIMutI CAGTG 5 cut(s) 157, 157, 182, 300, 451
Cac8I GCNNGC 1 cut(s) 221
CfoI GCGC 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 12
CseI GACGC 1 cut(s) 189
Csp6I GTAC 2 cut(s) 196, 651
CviAII CATG 5 cut(s) 360, 408, 412, 533, 590
CviJI RGCY 8 cut(s) 133, 207, 223, 264, 299, 390, 560, 631
CviKI_1 RGCY 8 cut(s) 133, 207, 223, 264, 299, 390, 560, 631
CviQI GTAC 2 cut(s) 196, 651
DdeI CTNAG 2 cut(s) 118, 393
DpnI GATC 3 cut(s) 169, 478, 619
DpnII GATC 3 cut(s) 167, 476, 617
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 1 cut(s) 638
EarI CTCTTC 1 cut(s) 638
Ecl136II GAGCTC 1 cut(s) 390
Eco105I TACGTA 1 cut(s) 237
Eco130I CCWWGG 2 cut(s) 319, 646
Eco24I GRGCYC 2 cut(s) 392, 633
Eco47I GGWCC 1 cut(s) 12
Eco53kI GAGCTC 1 cut(s) 390
Eco57I CTGAAG 2 cut(s) 94, 158
Eco88I CYCGRG 1 cut(s) 383
EcoICRI GAGCTC 1 cut(s) 390
EcoO109I RGGNCCY 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 640
EcoT14I CCWWGG 2 cut(s) 319, 646
EcoT22I ATGCAT 2 cut(s) 413, 446
EcoT38I GRGCYC 2 cut(s) 392, 633
ErhI CCWWGG 2 cut(s) 319, 646
FaeI CATG 5 cut(s) 363, 411, 415, 536, 593
FaqI GGGAC 1 cut(s) 147
FatI CATG 5 cut(s) 359, 407, 411, 532, 589
FauI CCCGC 1 cut(s) 100
FbaI TGATCA 1 cut(s) 167
Fnu4HI GCNGC 4 cut(s) 214, 217, 259, 262
FokI GGATG 3 cut(s) 307, 594, 606
FriOI GRGCYC 2 cut(s) 392, 633
Fsp4HI GCNGC 4 cut(s) 214, 217, 259, 262
FspBI CTAG 1 cut(s) 637
GlaI GCGC 1 cut(s) 184
GluI GCNGC 4 cut(s) 214, 217, 259, 262
GsuI CTGGAG 1 cut(s) 327
HaeIII GGCC 2 cut(s) 223, 299
HgaI GACGC 1 cut(s) 189
HhaI GCGC 1 cut(s) 185
Hin1II CATG 5 cut(s) 363, 411, 415, 536, 593
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HinfI GANTC 1 cut(s) 120
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 1 cut(s) 40
Hpy188III TCNNGA 4 cut(s) 54, 72, 385, 637
Hpy8I GTNNAC 1 cut(s) 40
HpyAV CCTTC 3 cut(s) 3, 281, 413
HpyCH4III ACNGT 3 cut(s) 140, 161, 471
HpyCH4IV ACGT 3 cut(s) 188, 236, 370
HpyCH4V TGCA 4 cut(s) 150, 411, 444, 665
HpyF10VI GCNNNNNNNGC 2 cut(s) 213, 267
HpyF3I CTNAG 2 cut(s) 118, 393
HpySE526I ACGT 3 cut(s) 188, 236, 370
Hsp92II CATG 5 cut(s) 363, 411, 415, 536, 593
HspAI GCGC 1 cut(s) 183
Ksp22I TGATCA 1 cut(s) 167
Kzo9I GATC 3 cut(s) 167, 476, 617
LguI GCTCTTC 1 cut(s) 638
LmnI GCTCC 2 cut(s) 74, 557
Lsp1109I GCAGC 3 cut(s) 200, 203, 273
LweI GCATC 2 cut(s) 94, 572
MaeI CTAG 1 cut(s) 637
MaeII ACGT 3 cut(s) 188, 236, 370
MaeIII GTNAC 2 cut(s) 155, 177
MalI GATC 3 cut(s) 169, 478, 619
MboI GATC 3 cut(s) 167, 476, 617
MboII GAAGA 4 cut(s) 87, 341, 533, 625
MhlI GDGCHC 2 cut(s) 392, 633
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 6 cut(s) 64, 277, 345, 463, 516, 640
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 1 cut(s) 129
MnlI CCTC 5 cut(s) 138, 317, 346, 391, 421
Mox20I TGGCCA 1 cut(s) 299
Mph1103I ATGCAT 2 cut(s) 413, 446
MroXI GAANNNNTTC 1 cut(s) 672
MscI TGGCCA 1 cut(s) 299
MseI TTAA 4 cut(s) 244, 349, 402, 576
MslI CAYNNNNRTG 2 cut(s) 175, 412
Msp20I TGGCCA 1 cut(s) 299
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 2 cut(s) 213, 267
NdeII GATC 3 cut(s) 167, 476, 617
NlaIII CATG 5 cut(s) 363, 411, 415, 536, 593
NlaIV GGNNCC 1 cut(s) 559
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 2 cut(s) 155, 177
NsiI ATGCAT 2 cut(s) 413, 446
NspI RCATGY 2 cut(s) 411, 415
OliI CACNNNNGTG 1 cut(s) 175
PaeR7I CTCGAG 1 cut(s) 383
PciSI GCTCTTC 1 cut(s) 638
PdmI GAANNNNTTC 1 cut(s) 672
PkrI GCNGC 4 cut(s) 215, 218, 260, 263
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
Ppu21I YACGTR 1 cut(s) 237
PpuMI RGGWCCY 1 cut(s) 12
PshBI ATTAAT 1 cut(s) 244
Psp124BI GAGCTC 1 cut(s) 392
Psp5II RGGWCCY 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 559
PspPI GGNCC 1 cut(s) 12
PspPPI RGGWCCY 1 cut(s) 12
RsaI GTAC 2 cut(s) 197, 652
RsaNI GTAC 2 cut(s) 196, 651
RseI CAYNNNNRTG 2 cut(s) 175, 412
SacI GAGCTC 1 cut(s) 392
SapI GCTCTTC 1 cut(s) 638
SaqAI TTAA 4 cut(s) 244, 349, 402, 576
SatI GCNGC 4 cut(s) 214, 217, 259, 262
Sau3AI GATC 3 cut(s) 167, 476, 617
Sau96I GGNCC 1 cut(s) 12
SchI GAGTC 1 cut(s) 129
SduI GDGCHC 2 cut(s) 392, 633
SfaNI GCATC 2 cut(s) 94, 572
Sfr274I CTCGAG 1 cut(s) 383
SinI GGWCC 1 cut(s) 12
SlaI CTCGAG 1 cut(s) 383
SmiMI CAYNNNNRTG 2 cut(s) 175, 412
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
SnaBI TACGTA 1 cut(s) 237
Sse9I AATT 6 cut(s) 64, 277, 345, 463, 516, 640
SsiI CCGC 2 cut(s) 107, 259
SspMI CTAG 1 cut(s) 637
SstI GAGCTC 1 cut(s) 392
StyI CCWWGG 2 cut(s) 319, 646
TaaI ACNGT 3 cut(s) 140, 161, 471
TaiI ACGT 3 cut(s) 191, 239, 373
TaqI TCGA 1 cut(s) 384
TasI AATT 6 cut(s) 64, 277, 345, 463, 516, 640
TauI GCSGC 1 cut(s) 261
Tru1I TTAA 4 cut(s) 244, 349, 402, 576
Tru9I TTAA 4 cut(s) 244, 349, 402, 576
TscAI CASTG 5 cut(s) 157, 164, 182, 307, 458
TseFI GTSAC 2 cut(s) 155, 177
TseI GCWGC 3 cut(s) 213, 216, 261
Tsp45I GTSAC 2 cut(s) 155, 177
TspDTI ATGAA 1 cut(s) 262
TspRI CASTG 5 cut(s) 157, 164, 182, 307, 458
VpaK11BI GGWCC 1 cut(s) 12
VspI ATTAAT 1 cut(s) 244
XapI RAATTY 3 cut(s) 345, 516, 640
XbaI TCTAGA 1 cut(s) 636
XceI RCATGY 2 cut(s) 411, 415
XhoI CTCGAG 1 cut(s) 383
XmnI GAANNNNTTC 1 cut(s) 672
XspI CTAG 1 cut(s) 637
Zsp2I ATGCAT 2 cut(s) 413, 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.