Rmu_sc0032878.1_g000001
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0032878.1
N/A
Physical Location & Seq
Forward (+)
139 .. 2960
2822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0032878.1_g000001.1.cds

Sequence Viewer

Length: 1398 bp
atgcgtcagattgctgagagctatattggatcaactgtgaaaaatatagtcatcactgtccctgcatatttcaatgacgcgcaacgtcaagctaccaaaaatgctggtgttactgcaggcctaaatgtgattcgtataatcaatgaaccaactgctgcagccattgcatatggacttgacaagaaggctgatttgtattgtaagagaaatattatgatatttgatttgggcggcggtactttagatgtctcattgctcaacattggagatggtgtctttgaagtgaaggctaccgctggtgatactcaccttggaggtgaggacttcaataacagaatggtgaattattgtgttgagcaattcaagaggaagcataatgtagatctcagtggaaactctagagctctcaggaggctacgaaatgaatgtgagaaggcaaagaggaggctttcatattcctcttcaattggcattgaagttgactgtttgaatgagggtattgatttctctactactattactcgtgccaaatttgaagatctcaacagggatttcttcaacaagtgtatggagcctgtggagaagtgtttgagtgatgctaacatggacacaagtagtgtcgatgacattgttcttgttggtggctcttctagagttccaaaggtacagcagctattgcagaatttcttcaaggggaaggagttgcgcaagggcattaatcctgatgaggcagtggcttatggtgctgctgttcaagctgccattttaagtgggaatagaaatgagaagatttgtgatataagcctcttggatgtcacacctctgtcacttggcatggcgagtggcacacaacgtgccatgggagtttttattccaagaaactcaagaattcccattactgtgaagcgaacttccactactacatctaatggacaattgtttatgctgaggacaagtccaccggtcggaagagagggattacaattaacagtgaccggagatcttgagcgcgtcaggatcggcgtcgtggtgatcgaattctccttatctgtggtggtttgtttgggcttggagcagcgtctggtatttgaggttgtcggggaggcagcgtaccttgatttgggcttctgggtcaacttgattctagtctacaacggcttcagtgctgtcctcatgcttgctcggcgaggcagaccacgggagttgagggcggtggtaactagttggcctcaggggcgacgaaggactctggccagccagtttgcttggatttgtgcctttggttatgggctcaggtcatgggggctcattaacccatgggctcaattgagcaggcttgattctctgcagaattgggctagtttggagggtgctttaacaccctcattcatcagttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

465

Amino Acids

51.21

Weight (kDa)

9.07

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 707
AccI GTMKAC 1 cut(s) 1149
AccII CGCG 2 cut(s) 80, 1011
AciI CCGC 4 cut(s) 231, 234, 294, 1211
AclWI GGATC 2 cut(s) 37, 1025
AcoI YGGCCR 1 cut(s) 1251
AcsI RAATTY 4 cut(s) 530, 682, 888, 1037
AcuI CTGAAG 1 cut(s) 1144
AcyI GRCGYC 1 cut(s) 1023
AdeI CACNNNGTG 1 cut(s) 854
AfaI GTAC 3 cut(s) 238, 666, 1112
AfiI CCNNNNNNNGG 2 cut(s) 965, 1120
AgeI ACCGGT 1 cut(s) 961
AhlI ACTAGT 1 cut(s) 1220
AluBI AGCT 5 cut(s) 21, 92, 404, 673, 758
AluI AGCT 5 cut(s) 21, 92, 404, 673, 758
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 1 cut(s) 253
AlwI GGATC 2 cut(s) 37, 1025
AlwNI CAGNNNCTG 1 cut(s) 1081
AoxI GGCC 3 cut(s) 118, 1226, 1251
ApeKI GCWGC 7 cut(s) 155, 158, 670, 746, 758, 1075, 1106
ApoI RAATTY 4 cut(s) 530, 682, 888, 1037
AseI ATTAAT 1 cut(s) 717
AsiGI ACCGGT 1 cut(s) 961
Asp700I GAANNNNTTC 1 cut(s) 686
AspLEI GCGC 3 cut(s) 82, 708, 1011
AsuHPI GGTGA 5 cut(s) 299, 311, 329, 352, 1042
AxyI CCTNAGG 1 cut(s) 1230
BalI TGGCCA 1 cut(s) 1253
BanII GRGCYC 4 cut(s) 406, 1293, 1308, 1324
BauI CACGAG 1 cut(s) 522
Bbv12I GWGCWC 1 cut(s) 406
BbvCI CCTCAGC 1 cut(s) 947
BbvI GCAGC 7 cut(s) 142, 170, 682, 733, 745, 1087, 1118
BccI CCATC 1 cut(s) 263
BceAI ACGGC 1 cut(s) 1171
BcoDI GTCTC 1 cut(s) 253
BcuI ACTAGT 1 cut(s) 1220
BfaI CTAG 5 cut(s) 399, 651, 1145, 1221, 1359
BfmI CTRYAG 3 cut(s) 114, 156, 1346
BglI GCCNNNNNGGC 1 cut(s) 1234
BglII AGATCT 3 cut(s) 382, 538, 1000
BisI GCNGC 8 cut(s) 156, 159, 232, 671, 747, 759, 1076, 1107
BlsI GCNGC 8 cut(s) 157, 160, 233, 672, 748, 760, 1077, 1108
BmiI GGNNCC 1 cut(s) 573
BmsI GCATC 1 cut(s) 586
Bpu10I CCTNAGC 2 cut(s) 947, 1292
BpuEI CTTGAG 2 cut(s) 868, 1025
BsaHI GRCGYC 1 cut(s) 1023
BsaJI CCNNGG 4 cut(s) 310, 858, 1196, 1316
BsaWI WCCGGW 2 cut(s) 961, 995
BsaXI ACNNNNNCTCC 4 cut(s) 258, 288, 1094, 1124
Bsc4I CCNNNNNNNGG 2 cut(s) 965, 1120
Bse118I RCCGGY 1 cut(s) 961
Bse1I ACTGG 1 cut(s) 1258
Bse21I CCTNAGG 1 cut(s) 1230
Bse3DI GCAATG 2 cut(s) 162, 251
BseDI CCNNGG 4 cut(s) 310, 858, 1196, 1316
BseGI GGATG 1 cut(s) 817
BseLI CCNNNNNNNGG 2 cut(s) 965, 1120
BseMI GCAATG 2 cut(s) 162, 251
BseMII CTCAG 6 cut(s) 6, 400, 421, 938, 1244, 1306
BseNI ACTGG 1 cut(s) 1258
BseRI GAGGAG 1 cut(s) 457
BseXI GCAGC 7 cut(s) 142, 170, 682, 733, 745, 1087, 1118
Bsh1236I CGCG 2 cut(s) 80, 1011
Bsh1285I CGRYCG 1 cut(s) 966
BshFI GGCC 3 cut(s) 120, 1228, 1253
BshTI ACCGGT 1 cut(s) 961
BsiEI CGRYCG 1 cut(s) 966
BsiHKAI GWGCWC 1 cut(s) 406
BsiSI CCGG 2 cut(s) 962, 996
BslFI GGGAC 1 cut(s) 44
BslI CCNNNNNNNGG 2 cut(s) 965, 1120
BsmAI GTCTC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 44
BsnI GGCC 3 cut(s) 120, 1228, 1253
Bsp1286I GDGCHC 4 cut(s) 406, 1293, 1308, 1324
Bsp143I GATC 6 cut(s) 29, 382, 538, 1000, 1017, 1032
Bsp19I CCATGG 2 cut(s) 858, 1316
BspACI CCGC 4 cut(s) 231, 234, 294, 1211
BspANI GGCC 3 cut(s) 120, 1228, 1253
BspCNI CTCAG 6 cut(s) 7, 399, 420, 939, 1243, 1305
BspFNI CGCG 2 cut(s) 80, 1011
BspLI GGNNCC 1 cut(s) 573
BspMAI CTGCAG 3 cut(s) 118, 160, 1350
BspPI GGATC 2 cut(s) 37, 1025
BspQI GCTCTTC 1 cut(s) 652
BsrDI GCAATG 2 cut(s) 162, 251
BsrFI RCCGGY 1 cut(s) 961
BsrI ACTGG 1 cut(s) 1258
BssAI RCCGGY 1 cut(s) 961
BssECI CCNNGG 4 cut(s) 310, 858, 1196, 1316
BssMI GATC 6 cut(s) 29, 382, 538, 1000, 1017, 1032
BssNI GRCGYC 1 cut(s) 1023
BssSI CACGAG 1 cut(s) 522
BssT1I CCWWGG 3 cut(s) 310, 858, 1316
Bst2BI CACGAG 1 cut(s) 522
Bst4CI ACNGT 5 cut(s) 37, 58, 485, 901, 991
Bst6I CTCTTC 3 cut(s) 466, 652, 964
BstACI GRCGYC 1 cut(s) 1023
BstAPI GCANNNNNTGC 2 cut(s) 164, 676
BstC8I GCNNGC 4 cut(s) 118, 1179, 1255, 1334
BstDEI CTNAG 6 cut(s) 15, 386, 407, 947, 1230, 1292
BstDSI CCRYGG 3 cut(s) 858, 1196, 1316
BstF5I GGATG 1 cut(s) 817
BstFNI CGCG 2 cut(s) 80, 1011
BstHHI GCGC 3 cut(s) 82, 708, 1011
BstKTI GATC 6 cut(s) 32, 385, 541, 1003, 1020, 1035
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 6 cut(s) 29, 382, 538, 1000, 1017, 1032
BstMCI CGRYCG 1 cut(s) 966
BstMWI GCNNNNNNNGC 6 cut(s) 164, 676, 743, 755, 1183, 1234
BstSFI CTRYAG 3 cut(s) 114, 156, 1346
BstUI CGCG 2 cut(s) 80, 1011
BstV1I GCAGC 7 cut(s) 142, 170, 682, 733, 745, 1087, 1118
BstX2I RGATCY 3 cut(s) 382, 538, 1000
BstYI RGATCY 3 cut(s) 382, 538, 1000
Bsu36I CCTNAGG 1 cut(s) 1230
BsuRI GGCC 3 cut(s) 120, 1228, 1253
BtgI CCRYGG 3 cut(s) 858, 1196, 1316
BtsCI GGATG 1 cut(s) 817
BtsI GCAGTG 1 cut(s) 738
BtsIMutI CAGTG 5 cut(s) 54, 394, 738, 996, 1168
Cac8I GCNNGC 4 cut(s) 118, 1179, 1255, 1334
CaiI CAGNNNCTG 1 cut(s) 1081
CfoI GCGC 3 cut(s) 82, 708, 1011
Cfr10I RCCGGY 1 cut(s) 961
CseI GACGC 4 cut(s) 86, 1000, 1012, 1067
Csp6I GTAC 3 cut(s) 237, 665, 1111
CspAI ACCGGT 1 cut(s) 961
CspCI CAANNNNNGTGG 2 cut(s) 1185, 1220
CviAII CATG 6 cut(s) 604, 835, 859, 1174, 1299, 1317
CviQI GTAC 3 cut(s) 237, 665, 1111
DdeI CTNAG 6 cut(s) 15, 386, 407, 947, 1230, 1292
DpnI GATC 6 cut(s) 31, 384, 540, 1002, 1019, 1034
DpnII GATC 6 cut(s) 29, 382, 538, 1000, 1017, 1032
DraIII CACNNNGTG 1 cut(s) 854
EaeI YGGCCR 1 cut(s) 1251
Eam1104I CTCTTC 3 cut(s) 466, 652, 964
EarI CTCTTC 3 cut(s) 466, 652, 964
Ecl136II GAGCTC 1 cut(s) 404
Eco130I CCWWGG 3 cut(s) 310, 858, 1316
Eco147I AGGCCT 1 cut(s) 120
Eco24I GRGCYC 4 cut(s) 406, 1293, 1308, 1324
Eco53kI GAGCTC 1 cut(s) 404
Eco57I CTGAAG 1 cut(s) 1144
Eco81I CCTNAGG 1 cut(s) 1230
EcoICRI GAGCTC 1 cut(s) 404
EcoRI GAATTC 2 cut(s) 888, 1037
EcoT14I CCWWGG 3 cut(s) 310, 858, 1316
EcoT38I GRGCYC 4 cut(s) 406, 1293, 1308, 1324
ErhI CCWWGG 3 cut(s) 310, 858, 1316
FaeI CATG 6 cut(s) 607, 838, 862, 1177, 1302, 1320
FaqI GGGAC 1 cut(s) 44
FatI CATG 6 cut(s) 603, 834, 858, 1173, 1298, 1316
FauNDI CATATG 1 cut(s) 169
FblI GTMKAC 1 cut(s) 1149
Fnu4HI GCNGC 8 cut(s) 156, 159, 232, 671, 747, 759, 1076, 1107
FokI GGATG 1 cut(s) 824
FriOI GRGCYC 4 cut(s) 406, 1293, 1308, 1324
Fsp4HI GCNGC 8 cut(s) 156, 159, 232, 671, 747, 759, 1076, 1107
FspBI CTAG 5 cut(s) 399, 651, 1145, 1221, 1359
FspI TGCGCA 1 cut(s) 707
GlaI GCGC 3 cut(s) 81, 707, 1010
GluI GCNGC 8 cut(s) 156, 159, 232, 671, 747, 759, 1076, 1107
HaeIII GGCC 3 cut(s) 120, 1228, 1253
HapII CCGG 2 cut(s) 962, 996
HgaI GACGC 4 cut(s) 86, 1000, 1012, 1067
HhaI GCGC 3 cut(s) 82, 708, 1011
Hin1I GRCGYC 1 cut(s) 1023
Hin1II CATG 6 cut(s) 607, 838, 862, 1177, 1302, 1320
Hin6I GCGC 3 cut(s) 80, 706, 1009
HinP1I GCGC 3 cut(s) 80, 706, 1009
HincII GTYRAC 2 cut(s) 481, 1135
HindII GTYRAC 2 cut(s) 481, 1135
HinfI GANTC 4 cut(s) 130, 1141, 1246, 1340
HpaII CCGG 2 cut(s) 962, 996
HphI GGTGA 5 cut(s) 299, 311, 329, 352, 1042
Hpy166II GTNNAC 4 cut(s) 481, 959, 1135, 1150
Hpy188I TCNGA 2 cut(s) 9, 968
Hpy188III TCNNGA 8 cut(s) 364, 399, 409, 651, 722, 885, 1004, 1015
Hpy8I GTNNAC 4 cut(s) 481, 959, 1135, 1150
Hpy99I CGWCG 2 cut(s) 1028, 1242
HpyAV CCTTC 5 cut(s) 178, 280, 427, 691, 1236
HpyCH4III ACNGT 5 cut(s) 37, 58, 485, 901, 991
HpyCH4IV ACGT 2 cut(s) 85, 853
HpyCH4V TGCA 6 cut(s) 65, 116, 158, 167, 679, 1348
HpyF10VI GCNNNNNNNGC 6 cut(s) 164, 676, 743, 755, 1183, 1234
HpyF3I CTNAG 6 cut(s) 15, 386, 407, 947, 1230, 1292
HpySE526I ACGT 2 cut(s) 85, 853
Hsp92I GRCGYC 1 cut(s) 1023
Hsp92II CATG 6 cut(s) 607, 838, 862, 1177, 1302, 1320
HspAI GCGC 3 cut(s) 80, 706, 1009
Kzo9I GATC 6 cut(s) 29, 382, 538, 1000, 1017, 1032
LguI GCTCTTC 1 cut(s) 652
LmnI GCTCC 2 cut(s) 571, 1072
Lsp1109I GCAGC 7 cut(s) 142, 170, 682, 733, 745, 1087, 1118
LweI GCATC 1 cut(s) 586
MaeI CTAG 5 cut(s) 399, 651, 1145, 1221, 1359
MaeII ACGT 2 cut(s) 85, 853
MaeIII GTNAC 5 cut(s) 109, 814, 825, 991, 1216
MalI GATC 6 cut(s) 31, 384, 540, 1002, 1019, 1034
MboI GATC 6 cut(s) 29, 382, 538, 1000, 1017, 1032
MboII GAAGA 7 cut(s) 453, 547, 548, 639, 679, 799, 981
MfeI CAATTG 3 cut(s) 465, 935, 1325
MflI RGATCY 3 cut(s) 382, 538, 1000
MhlI GDGCHC 4 cut(s) 406, 1293, 1308, 1324
MlsI TGGCCA 1 cut(s) 1253
MluNI TGGCCA 1 cut(s) 1253
MlyI GAGTC 1 cut(s) 1240
MmeI TCCRAC 1 cut(s) 946
Mox20I TGGCCA 1 cut(s) 1253
MroXI GAANNNNTTC 1 cut(s) 686
MscI TGGCCA 1 cut(s) 1253
MseI TTAA 5 cut(s) 717, 767, 986, 1311, 1376
MslI CAYNNNNRTG 1 cut(s) 899
Msp20I TGGCCA 1 cut(s) 1253
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 2 cut(s) 962, 996
MunI CAATTG 3 cut(s) 465, 935, 1325
MvnI CGCG 2 cut(s) 80, 1011
MwoI GCNNNNNNNGC 6 cut(s) 164, 676, 743, 755, 1183, 1234
NcoI CCATGG 2 cut(s) 858, 1316
NdeI CATATG 1 cut(s) 169
NdeII GATC 6 cut(s) 29, 382, 538, 1000, 1017, 1032
NlaIII CATG 6 cut(s) 607, 838, 862, 1177, 1302, 1320
NlaIV GGNNCC 1 cut(s) 573
NmeAIII GCCGAG 1 cut(s) 1162
NmuCI GTSAC 3 cut(s) 814, 825, 991
NsbI TGCGCA 1 cut(s) 707
PceI AGGCCT 1 cut(s) 120
PciSI GCTCTTC 1 cut(s) 652
PcsI WCGNNNNNNNCGW 1 cut(s) 1032
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 3 cut(s) 130, 1141, 1340
PinAI ACCGGT 1 cut(s) 961
PkrI GCNGC 8 cut(s) 157, 160, 233, 672, 748, 760, 1077, 1108
PleI GAGTC 1 cut(s) 1240
PpsI GAGTC 1 cut(s) 1240
PshBI ATTAAT 1 cut(s) 717
Psp124BI GAGCTC 1 cut(s) 406
PspN4I GGNNCC 1 cut(s) 573
PsrI GAACNNNNNNTAC 2 cut(s) 901, 933
PstI CTGCAG 3 cut(s) 118, 160, 1350
PstNI CAGNNNCTG 1 cut(s) 1081
PsuI RGATCY 3 cut(s) 382, 538, 1000
RsaI GTAC 3 cut(s) 238, 666, 1112
RsaNI GTAC 3 cut(s) 237, 665, 1111
RseI CAYNNNNRTG 1 cut(s) 899
SacI GAGCTC 1 cut(s) 406
SapI GCTCTTC 1 cut(s) 652
SaqAI TTAA 5 cut(s) 717, 767, 986, 1311, 1376
SatI GCNGC 8 cut(s) 156, 159, 232, 671, 747, 759, 1076, 1107
Sau3AI GATC 6 cut(s) 29, 382, 538, 1000, 1017, 1032
SchI GAGTC 1 cut(s) 1240
SduI GDGCHC 4 cut(s) 406, 1293, 1308, 1324
SfaNI GCATC 1 cut(s) 586
SfcI CTRYAG 3 cut(s) 114, 156, 1346
SmiMI CAYNNNNRTG 1 cut(s) 899
SmlI CTYRAG 2 cut(s) 883, 1004
SmoI CTYRAG 2 cut(s) 883, 1004
SpeI ACTAGT 1 cut(s) 1220
SseBI AGGCCT 1 cut(s) 120
SsiI CCGC 4 cut(s) 231, 234, 294, 1211
SspI AATATT 1 cut(s) 211
SspMI CTAG 5 cut(s) 399, 651, 1145, 1221, 1359
SstI GAGCTC 1 cut(s) 406
StuI AGGCCT 1 cut(s) 120
StyI CCWWGG 3 cut(s) 310, 858, 1316
TaaI ACNGT 5 cut(s) 37, 58, 485, 901, 991
TaiI ACGT 2 cut(s) 88, 856
TaqI TCGA 2 cut(s) 621, 1035
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 3 cut(s) 130, 1141, 1340
Tru1I TTAA 5 cut(s) 717, 767, 986, 1311, 1376
Tru9I TTAA 5 cut(s) 717, 767, 986, 1311, 1376
TscAI CASTG 5 cut(s) 61, 394, 738, 996, 1168
TseFI GTSAC 3 cut(s) 814, 825, 991
TseI GCWGC 7 cut(s) 155, 158, 670, 746, 758, 1075, 1106
Tsp45I GTSAC 3 cut(s) 814, 825, 991
TspDTI ATGAA 4 cut(s) 159, 438, 441, 1378
TspRI CASTG 5 cut(s) 61, 394, 738, 996, 1168
VspI ATTAAT 1 cut(s) 717
XapI RAATTY 4 cut(s) 530, 682, 888, 1037
XbaI TCTAGA 2 cut(s) 398, 650
XmiI GTMKAC 1 cut(s) 1149
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 5 cut(s) 399, 651, 1145, 1221, 1359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.