Rmu_ssc0000058.1_g000008

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000058.1
N/A
Physical Location & Seq
Reverse (-)
42440 .. 42817
378 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 378 bp
atgggtcagtcaggggggctgggactattttggatggatgatgtcaaggtccatttccagtcaaaatctgagtggcatattgacgttgtcgtggaggatggttgcggtggtcaacggtggaggctcacttgcttcttcggccgtgttgcagcaacagaaaggcttcaatcatggaatctgctgaaggcattgggtgatgcatactcattgctgtgggttgtgataagtgatttcaatgaaatattaaattcgtcggaaaagcttggtgatagacaatgtcctgaagatcaaatgcagcggttccgtgatgctttggcttatactaatctgatcgacttagggtactttggagtgcctttcattggagctaccctataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283

Protein Analysis

125

Amino Acids

14.17

Weight (kDa)

4.85

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 105, 298
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 1 cut(s) 247
AcuI CTGAAG 2 cut(s) 203, 303
AfaI GTAC 1 cut(s) 344
AfiI CCNNNNNNNGG 1 cut(s) 362
AgsI TTSAA 2 cut(s) 167, 235
AluBI AGCT 2 cut(s) 262, 368
AluI AGCT 2 cut(s) 262, 368
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 149, 295
ApoI RAATTY 1 cut(s) 247
Asp700I GAANNNNTTC 1 cut(s) 162
AspS9I GGNCC 1 cut(s) 49
AsuHPI GGTGA 2 cut(s) 206, 278
AvaII GGWCC 1 cut(s) 49
BbvI GCAGC 2 cut(s) 161, 307
BccI CCATC 2 cut(s) 28, 92
BceAI ACGGC 1 cut(s) 126
BisI GCNGC 2 cut(s) 150, 296
BlsI GCNGC 2 cut(s) 151, 297
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 1 cut(s) 302
BmsI GCATC 2 cut(s) 187, 298
Bsc4I CCNNNNNNNGG 1 cut(s) 362
Bse1I ACTGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 206
BseGI GGATG 3 cut(s) 39, 43, 103
BseLI CCNNNNNNNGG 1 cut(s) 362
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 58
BseX3I CGGCCG 1 cut(s) 139
BseXI GCAGC 2 cut(s) 161, 307
BseYI CCCAGC 1 cut(s) 19
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 1 cut(s) 141
BsiEI CGRYCG 1 cut(s) 142
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 362
BsmFI GGGAC 1 cut(s) 36
BsnI GGCC 1 cut(s) 141
Bsp143I GATC 2 cut(s) 286, 330
BspACI CCGC 2 cut(s) 105, 298
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 206
BsrI ACTGG 1 cut(s) 58
BssMI GATC 2 cut(s) 286, 330
Bst4CI ACNGT 1 cut(s) 117
BstDEI CTNAG 2 cut(s) 69, 337
BstF5I GGATG 3 cut(s) 39, 43, 103
BstKTI GATC 2 cut(s) 289, 333
BstMBI GATC 2 cut(s) 286, 330
BstMCI CGRYCG 1 cut(s) 142
BstMWI GCNNNNNNNGC 1 cut(s) 138
BstV1I GCAGC 2 cut(s) 161, 307
BstZI CGGCCG 1 cut(s) 139
BsuRI GGCC 1 cut(s) 141
BtsCI GGATG 3 cut(s) 39, 43, 103
Cfr13I GGNCC 1 cut(s) 49
Csp6I GTAC 1 cut(s) 343
CviAII CATG 1 cut(s) 171
CviJI RGCY 7 cut(s) 19, 124, 141, 163, 262, 317, 368
CviKI_1 RGCY 7 cut(s) 19, 124, 141, 163, 262, 317, 368
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 2 cut(s) 69, 337
DpnI GATC 2 cut(s) 288, 332
DpnII GATC 2 cut(s) 286, 330
EaeI YGGCCR 1 cut(s) 139
EagI CGGCCG 1 cut(s) 139
EclXI CGGCCG 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 49
Eco52I CGGCCG 1 cut(s) 139
Eco57I CTGAAG 2 cut(s) 203, 303
EcoT22I ATGCAT 1 cut(s) 202
FaeI CATG 1 cut(s) 174
FaiI YATR 5 cut(s) 78, 172, 202, 321, 376
FaqI GGGAC 1 cut(s) 36
FatI CATG 1 cut(s) 170
Fnu4HI GCNGC 2 cut(s) 150, 296
FokI GGATG 3 cut(s) 46, 50, 110
Fsp4HI GCNGC 2 cut(s) 150, 296
GluI GCNGC 2 cut(s) 150, 296
GsaI CCCAGC 1 cut(s) 23
HaeIII GGCC 1 cut(s) 141
Hin1II CATG 1 cut(s) 174
HincII GTYRAC 1 cut(s) 113
HindII GTYRAC 1 cut(s) 113
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 1 cut(s) 175
HphI GGTGA 2 cut(s) 206, 278
Hpy166II GTNNAC 1 cut(s) 113
Hpy188I TCNGA 3 cut(s) 70, 256, 330
Hpy188III TCNNGA 1 cut(s) 281
Hpy8I GTNNAC 1 cut(s) 113
Hpy99I CGWCG 1 cut(s) 256
HpyAV CCTTC 1 cut(s) 178
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 3 cut(s) 149, 200, 295
HpyF10VI GCNNNNNNNGC 1 cut(s) 138
HpyF3I CTNAG 2 cut(s) 69, 337
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 1 cut(s) 174
Kzo9I GATC 2 cut(s) 286, 330
LmnI GCTCC 1 cut(s) 365
LpnPI CCDG 3 cut(s) 5, 71, 294
Lsp1109I GCAGC 2 cut(s) 161, 307
LweI GCATC 2 cut(s) 187, 298
MaeII ACGT 1 cut(s) 84
MalI GATC 2 cut(s) 288, 332
MboI GATC 2 cut(s) 286, 330
MboII GAAGA 2 cut(s) 127, 296
MluCI AATT 1 cut(s) 247
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 2 cut(s) 88, 114
Mph1103I ATGCAT 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 162
MseI TTAA 1 cut(s) 245
MslI CAYNNNNRTG 1 cut(s) 211
MspA1I CMGCKG 1 cut(s) 298
MwoI GCNNNNNNNGC 1 cut(s) 138
NdeII GATC 2 cut(s) 286, 330
NlaIII CATG 1 cut(s) 174
NlaIV GGNNCC 1 cut(s) 302
NsiI ATGCAT 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 162
PfeI GAWTC 1 cut(s) 175
PflFI GACNNNGTC 2 cut(s) 86, 276
PkrI GCNGC 2 cut(s) 151, 297
PspFI CCCAGC 1 cut(s) 19
PspN4I GGNNCC 1 cut(s) 302
PspPI GGNCC 1 cut(s) 49
PsyI GACNNNGTC 2 cut(s) 86, 276
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
RseI CAYNNNNRTG 1 cut(s) 211
SaqAI TTAA 1 cut(s) 245
SatI GCNGC 2 cut(s) 150, 296
Sau3AI GATC 2 cut(s) 286, 330
Sau96I GGNCC 1 cut(s) 49
SetI ASST 4 cut(s) 51, 87, 264, 370
SfaNI GCATC 2 cut(s) 187, 298
SinI GGWCC 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 211
Sse9I AATT 1 cut(s) 247
SsiI CCGC 2 cut(s) 105, 298
SspI AATATT 1 cut(s) 243
TaaI ACNGT 1 cut(s) 117
TaiI ACGT 1 cut(s) 87
TaqI TCGA 1 cut(s) 333
TasI AATT 1 cut(s) 247
TfiI GAWTC 1 cut(s) 175
Tru1I TTAA 1 cut(s) 245
Tru9I TTAA 1 cut(s) 245
TseI GCWGC 2 cut(s) 149, 295
TspDTI ATGAA 2 cut(s) 252, 349
TspGWI ACGGA 1 cut(s) 293
Tth111I GACNNNGTC 2 cut(s) 86, 276
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 1 cut(s) 247
XmnI GAANNNNTTC 1 cut(s) 162
Zsp2I ATGCAT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.