Rmu_ssc0000067.1_g000041

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000067.1
N/A
Physical Location & Seq
Forward (+)
148943 .. 150346
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000067.1_g000041.1.cds

Sequence Viewer

Length: 477 bp
atggttggcattttagatgtcgggggaagtgatgttcgcatggttgttatatgggatattggtgggctagggaaaacaacaattgctaaggctatatgcaactcaattgcccataagtttgaaggttggtgtttcttgggaaatgttagagcagcttcagtaccccatggaggcctagttcaactacaaaacaatcttcttaatgagattttaggaggccggaaaatggagatgaccaatgctgatagaggaatccagccccgatttgttgtcgtcgaggctttgagccactacgagttcatggaccagagctctgacgacgccgcagctggcaatgaattttggatcgagttgtcgccaaatacgatcaccgccggggatgaggtggtgatgagtttggcgctggcgagtgagagagctgcaatgctgccagaggtggaggtagaagggctgcttctgtccaactcgtttttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5 NB-ARC TIR

Protein Analysis

158

Amino Acids

17.03

Weight (kDa)

4.57

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 324, 372
AclWI GGATC 1 cut(s) 353
AcsI RAATTY 1 cut(s) 338
AcuI CTGAAG 1 cut(s) 141
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 1 cut(s) 162
AfiI CCNNNNNNNGG 2 cut(s) 170, 226
AgsI TTSAA 2 cut(s) 122, 182
AjuI GAANNNNNNNTTGG 1 cut(s) 455
AloI GAACNNNNNNTCC 4 cut(s) 18, 50, 162, 194
AluBI AGCT 4 cut(s) 155, 312, 329, 419
AluI AGCT 4 cut(s) 155, 312, 329, 419
Alw21I GWGCWC 1 cut(s) 314
AlwI GGATC 1 cut(s) 353
AoxI GGCC 2 cut(s) 172, 217
ApeKI GCWGC 5 cut(s) 152, 326, 419, 427, 451
ApoI RAATTY 1 cut(s) 338
AspLEI GCGC 1 cut(s) 403
AspS9I GGNCC 1 cut(s) 304
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 2 cut(s) 361, 400
AvaII GGWCC 1 cut(s) 304
BanII GRGCYC 1 cut(s) 314
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 5 cut(s) 164, 338, 406, 414, 438
BcnI CCSGG 1 cut(s) 376
BfaI CTAG 3 cut(s) 68, 176, 475
BfoI RGCGCY 1 cut(s) 404
BisI GCNGC 6 cut(s) 153, 324, 327, 420, 428, 452
BlsI GCNGC 6 cut(s) 154, 325, 328, 421, 429, 453
Bme1390I CCNGG 1 cut(s) 376
Bme18I GGWCC 1 cut(s) 304
BmgT120I GGNCC 1 cut(s) 304
BmrFI CCNGG 1 cut(s) 376
Bpu10I CCTNAGC 1 cut(s) 87
BpuMI CCSGG 1 cut(s) 376
BsaHI GRCGYC 1 cut(s) 321
BsaJI CCNNGG 2 cut(s) 166, 375
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 226
Bse3DI GCAATG 2 cut(s) 340, 429
BseDI CCNNGG 2 cut(s) 166, 375
BseGI GGATG 1 cut(s) 385
BseLI CCNNNNNNNGG 2 cut(s) 170, 226
BseMI GCAATG 2 cut(s) 340, 429
BseXI GCAGC 5 cut(s) 164, 338, 406, 414, 438
BshFI GGCC 2 cut(s) 174, 219
BsiHKAI GWGCWC 1 cut(s) 314
BsiSI CCGG 2 cut(s) 220, 375
BslI CCNNNNNNNGG 2 cut(s) 170, 226
BsnI GGCC 2 cut(s) 174, 219
Bsp1286I GDGCHC 1 cut(s) 314
Bsp143I GATC 2 cut(s) 345, 366
Bsp19I CCATGG 1 cut(s) 166
BspACI CCGC 2 cut(s) 324, 372
BspANI GGCC 2 cut(s) 174, 219
BspPI GGATC 1 cut(s) 353
BsrDI GCAATG 2 cut(s) 340, 429
BssECI CCNNGG 2 cut(s) 166, 375
BssMI GATC 2 cut(s) 345, 366
BssNI GRCGYC 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 166
BstACI GRCGYC 1 cut(s) 321
BstC8I GCNNGC 2 cut(s) 331, 405
BstDEI CTNAG 1 cut(s) 87
BstDSI CCRYGG 1 cut(s) 166
BstF5I GGATG 1 cut(s) 385
BstH2I RGCGCY 1 cut(s) 404
BstHHI GCGC 1 cut(s) 403
BstKTI GATC 2 cut(s) 348, 369
BstMBI GATC 2 cut(s) 345, 366
BstSCI CCNGG 1 cut(s) 374
BstV1I GCAGC 5 cut(s) 164, 338, 406, 414, 438
BsuRI GGCC 2 cut(s) 174, 219
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 1 cut(s) 385
Cac8I GCNNGC 2 cut(s) 331, 405
CfoI GCGC 1 cut(s) 403
Cfr13I GGNCC 1 cut(s) 304
CseI GACGC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 161
CviAII CATG 3 cut(s) 40, 167, 301
CviQI GTAC 1 cut(s) 161
DdeI CTNAG 1 cut(s) 87
DpnI GATC 2 cut(s) 347, 368
DpnII GATC 2 cut(s) 345, 366
Ecl136II GAGCTC 1 cut(s) 312
Eco130I CCWWGG 1 cut(s) 166
Eco147I AGGCCT 1 cut(s) 174
Eco24I GRGCYC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 304
Eco53kI GAGCTC 1 cut(s) 312
Eco57I CTGAAG 1 cut(s) 141
EcoICRI GAGCTC 1 cut(s) 312
EcoT14I CCWWGG 1 cut(s) 166
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 166
FaeI CATG 3 cut(s) 43, 170, 304
FaiI YATR 8 cut(s) 41, 50, 52, 95, 97, 114, 168, 302
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FatI CATG 3 cut(s) 39, 166, 300
Fnu4HI GCNGC 6 cut(s) 153, 324, 327, 420, 428, 452
FokI GGATG 1 cut(s) 392
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 6 cut(s) 153, 324, 327, 420, 428, 452
FspBI CTAG 3 cut(s) 68, 176, 475
GlaI GCGC 1 cut(s) 402
GluI GCNGC 6 cut(s) 153, 324, 327, 420, 428, 452
HaeII RGCGCY 1 cut(s) 404
HaeIII GGCC 2 cut(s) 174, 219
HapII CCGG 2 cut(s) 220, 375
HgaI GACGC 1 cut(s) 329
HhaI GCGC 1 cut(s) 403
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 3 cut(s) 43, 170, 304
Hin6I GCGC 1 cut(s) 401
HinP1I GCGC 1 cut(s) 401
HinfI GANTC 1 cut(s) 252
HpaII CCGG 2 cut(s) 220, 375
HphI GGTGA 2 cut(s) 361, 400
Hpy188I TCNGA 1 cut(s) 316
Hpy99I CGWCG 2 cut(s) 278, 323
HpyAV CCTTC 2 cut(s) 116, 440
HpyCH4V TGCA 2 cut(s) 99, 422
HpyF3I CTNAG 1 cut(s) 87
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 3 cut(s) 43, 170, 304
HspAI GCGC 1 cut(s) 401
Kzo9I GATC 2 cut(s) 345, 366
LpnPI CCDG 7 cut(s) 233, 269, 315, 320, 388, 389, 444
Lsp1109I GCAGC 5 cut(s) 164, 338, 406, 414, 438
MaeI CTAG 3 cut(s) 68, 176, 475
MalI GATC 2 cut(s) 347, 368
MboI GATC 2 cut(s) 345, 366
MboII GAAGA 1 cut(s) 188
MfeI CAATTG 2 cut(s) 81, 105
MhlI GDGCHC 1 cut(s) 314
MluCI AATT 3 cut(s) 81, 105, 338
MnlI CCTC 7 cut(s) 164, 209, 242, 271, 376, 427, 433
MseI TTAA 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 2 cut(s) 220, 375
MspR9I CCNGG 1 cut(s) 376
MunI CAATTG 2 cut(s) 81, 105
NciI CCSGG 1 cut(s) 376
NcoI CCATGG 1 cut(s) 166
NdeII GATC 2 cut(s) 345, 366
NlaIII CATG 3 cut(s) 43, 170, 304
PceI AGGCCT 1 cut(s) 174
PcsI WCGNNNNNNNCGW 1 cut(s) 362
PfeI GAWTC 1 cut(s) 252
PkrI GCNGC 6 cut(s) 154, 325, 328, 421, 429, 453
Psp124BI GAGCTC 1 cut(s) 314
PspPI GGNCC 1 cut(s) 304
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 1 cut(s) 162
RsaNI GTAC 1 cut(s) 161
SacI GAGCTC 1 cut(s) 314
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 6 cut(s) 153, 324, 327, 420, 428, 452
Sau3AI GATC 2 cut(s) 345, 366
Sau96I GGNCC 1 cut(s) 304
ScrFI CCNGG 1 cut(s) 376
SduI GDGCHC 1 cut(s) 314
SetI ASST 8 cut(s) 127, 157, 314, 331, 387, 421, 438, 444
SinI GGWCC 1 cut(s) 304
Sse9I AATT 3 cut(s) 81, 105, 338
SseBI AGGCCT 1 cut(s) 174
SsiI CCGC 2 cut(s) 324, 372
SspMI CTAG 3 cut(s) 68, 176, 475
SstI GAGCTC 1 cut(s) 314
StuI AGGCCT 1 cut(s) 174
StyD4I CCNGG 1 cut(s) 374
StyI CCWWGG 1 cut(s) 166
TaqI TCGA 2 cut(s) 276, 348
TasI AATT 3 cut(s) 81, 105, 338
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 1 cut(s) 252
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TseI GCWGC 5 cut(s) 152, 326, 419, 427, 451
TspDTI ATGAA 2 cut(s) 289, 351
VpaK11BI GGWCC 1 cut(s) 304
XapI RAATTY 1 cut(s) 338
XspI CTAG 3 cut(s) 68, 176, 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.