Rmu_ssc0000075.1_g000004

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000075.1
N/A
Physical Location & Seq
Reverse (-)
24094 .. 26990
2897 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 738 bp
atggctgacatccgttttgagactgaggaggtagggctacagatggactcgagccttgtggtgttgggggctgccggggctgcagcgtttcaagatgggaactggtgcatggtgggccgtttgttggcgccgaaagcaaggctgcccggctttaagggaactattgccacgatctggaacatcagatcaggattgaccattcaggacgcaggggagcgatttgtgttccaatttgagacagaagctgtgcgcaaccggatcttgcgtggaggtccctggttttatcggaatactatgctagtggtgggagaatatgatggtctcagtcctgtggaggaggtgcctctaaatcttttggaggcctgggtggttgtgaagggacttccttttgccctgaggatgtgttgttatattcgtttaagagacaaactatggtcacccacgacgcctgctctcgccattgcggtgtcgaaactcgtcgtcgtgctctccgccgccaggctcgatgccaagccaacggaagcccagacccgattccggcgtggagagcttcccggacatctgacgaacaaaaccggcgaagcccagaagggttgggatcgtggagctagatggcgtcatcctctggtggtccgacggtgggacggagcggcgatggtggaggttgacatcgacggtgaagtggtgggcgtggtagctcagagagagagagagaaagagagcctgagacgaggttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

245

Amino Acids

27.32

Weight (kDa)

9.29

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 34 - 103 2.4e-09 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 251
AccB1I GGYRCC 2 cut(s) 127, 340
AccB7I CCANNNNNTGG 1 cut(s) 174
AccBSI CCGCTC 1 cut(s) 650
AciI CCGC 4 cut(s) 464, 492, 495, 650
AclWI GGATC 2 cut(s) 266, 606
AcyI GRCGYC 3 cut(s) 128, 446, 616
AfiI CCNNNNNNNGG 5 cut(s) 124, 174, 498, 537, 640
AgsI TTSAA 1 cut(s) 92
AjnI CCWGG 3 cut(s) 275, 362, 497
AluBI AGCT 4 cut(s) 245, 550, 608, 698
AluI AGCT 4 cut(s) 245, 550, 608, 698
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 5 cut(s) 14, 230, 326, 417, 721
AlwI GGATC 2 cut(s) 266, 606
AlwNI CAGNNNCTG 1 cut(s) 245
Ama87I CYCGRG 1 cut(s) 49
AoxI GGCC 2 cut(s) 115, 360
ApeKI GCWGC 4 cut(s) 71, 80, 83, 142
ArsI GACNNNNNNTTYG 1 cut(s) 31
AspLEI GCGC 2 cut(s) 130, 252
AspS9I GGNCC 3 cut(s) 115, 272, 631
AsuC2I CCSGG 3 cut(s) 76, 147, 555
AsuHPI GGTGA 2 cut(s) 429, 689
AvaI CYCGRG 1 cut(s) 49
AvaII GGWCC 2 cut(s) 272, 631
AxyI CCTNAGG 1 cut(s) 395
BanI GGYRCC 2 cut(s) 127, 340
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 4 cut(s) 58, 67, 95, 129
BccI CCATC 5 cut(s) 37, 89, 311, 606, 649
BceAI ACGGC 1 cut(s) 102
BciT130I CCWGG 3 cut(s) 277, 364, 499
BcnI CCSGG 3 cut(s) 76, 147, 555
BcoDI GTCTC 5 cut(s) 14, 230, 326, 417, 721
BfaI CTAG 2 cut(s) 299, 609
BfmI CTRYAG 2 cut(s) 38, 81
BfoI RGCGCY 1 cut(s) 131
BisI GCNGC 6 cut(s) 72, 81, 84, 143, 495, 651
BlsI GCNGC 6 cut(s) 73, 82, 85, 144, 496, 652
Bme1390I CCNGG 6 cut(s) 76, 147, 277, 364, 499, 555
Bme18I GGWCC 2 cut(s) 272, 631
BmeT110I CYCGRG 1 cut(s) 49
BmgT120I GGNCC 3 cut(s) 115, 272, 631
BmiI GGNNCC 3 cut(s) 129, 274, 342
BmrFI CCNGG 6 cut(s) 76, 147, 277, 364, 499, 555
BmsI GCATC 1 cut(s) 496
BpuMI CCSGG 3 cut(s) 76, 147, 555
BsaHI GRCGYC 3 cut(s) 128, 446, 616
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 3 cut(s) 75, 275, 363
BsaWI WCCGGW 1 cut(s) 255
Bsc4I CCNNNNNNNGG 5 cut(s) 124, 174, 498, 537, 640
Bse118I RCCGGY 1 cut(s) 575
Bse1I ACTGG 1 cut(s) 107
Bse21I CCTNAGG 1 cut(s) 395
Bse3DI GCAATG 1 cut(s) 459
BseBI CCWGG 3 cut(s) 277, 364, 499
BseDI CCNNGG 3 cut(s) 75, 275, 363
BseGI GGATG 3 cut(s) 9, 405, 619
BseLI CCNNNNNNNGG 5 cut(s) 124, 174, 498, 537, 640
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 5 cut(s) 15, 337, 386, 713, 716
BseNI ACTGG 1 cut(s) 107
BseRI GAGGAG 2 cut(s) 41, 350
BseXI GCAGC 4 cut(s) 58, 67, 95, 129
BshFI GGCC 2 cut(s) 117, 362
BshNI GGYRCC 2 cut(s) 127, 340
BsiHKAI GWGCWC 1 cut(s) 489
BsiHKCI CYCGRG 1 cut(s) 49
BsiSI CCGG 6 cut(s) 75, 147, 256, 538, 555, 576
BslFI GGGAC 3 cut(s) 258, 393, 656
BslI CCNNNNNNNGG 5 cut(s) 124, 174, 498, 537, 640
BsmAI GTCTC 5 cut(s) 14, 230, 326, 417, 721
BsmBI CGTCTC 1 cut(s) 721
BsmFI GGGAC 3 cut(s) 258, 393, 656
BsnI GGCC 2 cut(s) 117, 362
Bso31I GGTCTC 1 cut(s) 326
BsoBI CYCGRG 1 cut(s) 49
Bsp1286I GDGCHC 1 cut(s) 489
Bsp143I GATC 4 cut(s) 171, 185, 258, 598
BspACI CCGC 4 cut(s) 464, 492, 495, 650
BspANI GGCC 2 cut(s) 117, 362
BspCNI CTCAG 5 cut(s) 16, 336, 387, 712, 717
BspLI GGNNCC 3 cut(s) 129, 274, 342
BspMAI CTGCAG 1 cut(s) 85
BspPI GGATC 2 cut(s) 266, 606
BspT107I GGYRCC 2 cut(s) 127, 340
BspTNI GGTCTC 1 cut(s) 326
BsrBI CCGCTC 1 cut(s) 650
BsrDI GCAATG 1 cut(s) 459
BsrFI RCCGGY 1 cut(s) 575
BsrI ACTGG 1 cut(s) 107
BssAI RCCGGY 1 cut(s) 575
BssECI CCNNGG 3 cut(s) 75, 275, 363
BssMI GATC 4 cut(s) 171, 185, 258, 598
BssNI GRCGYC 3 cut(s) 128, 446, 616
Bst2UI CCWGG 3 cut(s) 277, 364, 499
Bst4CI ACNGT 2 cut(s) 639, 677
BstACI GRCGYC 3 cut(s) 128, 446, 616
BstC8I GCNNGC 1 cut(s) 450
BstDEI CTNAG 5 cut(s) 24, 323, 395, 699, 725
BstEII GGTNACC 1 cut(s) 435
BstF5I GGATG 3 cut(s) 9, 405, 619
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 2 cut(s) 130, 252
BstKTI GATC 4 cut(s) 174, 188, 261, 601
BstMAI GTCTC 5 cut(s) 14, 230, 326, 417, 721
BstMBI GATC 4 cut(s) 171, 185, 258, 598
BstMWI GCNNNNNNNGC 5 cut(s) 77, 80, 114, 134, 547
BstNI CCWGG 3 cut(s) 277, 364, 499
BstPI GGTNACC 1 cut(s) 435
BstSCI CCNGG 6 cut(s) 74, 145, 275, 362, 497, 553
BstSFI CTRYAG 2 cut(s) 38, 81
BstV1I GCAGC 4 cut(s) 58, 67, 95, 129
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
Bsu36I CCTNAGG 1 cut(s) 395
BsuRI GGCC 2 cut(s) 117, 362
BtgZI GCGATG 1 cut(s) 668
BtsCI GGATG 3 cut(s) 9, 405, 619
Cac8I GCNNGC 1 cut(s) 450
CaiI CAGNNNCTG 1 cut(s) 245
CfoI GCGC 2 cut(s) 130, 252
Cfr10I RCCGGY 1 cut(s) 575
Cfr13I GGNCC 3 cut(s) 115, 272, 631
CseI GACGC 3 cut(s) 215, 454, 605
CviAII CATG 1 cut(s) 109
DdeI CTNAG 5 cut(s) 24, 323, 395, 699, 725
DinI GGCGCC 1 cut(s) 129
DpnI GATC 4 cut(s) 173, 187, 260, 600
DpnII GATC 4 cut(s) 171, 185, 258, 598
EciI GGCGGA 1 cut(s) 481
Eco147I AGGCCT 1 cut(s) 362
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 2 cut(s) 272, 631
Eco81I CCTNAGG 1 cut(s) 395
Eco88I CYCGRG 1 cut(s) 49
Eco91I GGTNACC 1 cut(s) 435
EcoO109I RGGNCCY 1 cut(s) 272
EcoO65I GGTNACC 1 cut(s) 435
EcoRII CCWGG 3 cut(s) 275, 362, 497
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 721
FaeI CATG 1 cut(s) 112
FaiI YATR 5 cut(s) 110, 296, 315, 411, 433
FaqI GGGAC 3 cut(s) 258, 393, 656
FatI CATG 1 cut(s) 108
Fnu4HI GCNGC 6 cut(s) 72, 81, 84, 143, 495, 651
FokI GGATG 2 cut(s) 412, 606
Fsp4HI GCNGC 6 cut(s) 72, 81, 84, 143, 495, 651
FspBI CTAG 2 cut(s) 299, 609
FspI TGCGCA 1 cut(s) 251
GlaI GCGC 2 cut(s) 129, 251
GluI GCNGC 6 cut(s) 72, 81, 84, 143, 495, 651
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 2 cut(s) 117, 362
HapII CCGG 6 cut(s) 75, 147, 256, 538, 555, 576
HgaI GACGC 3 cut(s) 215, 454, 605
HhaI GCGC 2 cut(s) 130, 252
Hin1I GRCGYC 3 cut(s) 128, 446, 616
Hin1II CATG 1 cut(s) 112
Hin6I GCGC 2 cut(s) 128, 250
HinP1I GCGC 2 cut(s) 128, 250
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HinfI GANTC 2 cut(s) 47, 534
HpaII CCGG 6 cut(s) 75, 147, 256, 538, 555, 576
HphI GGTGA 2 cut(s) 429, 689
Hpy166II GTNNAC 1 cut(s) 667
Hpy188I TCNGA 5 cut(s) 185, 288, 564, 635, 702
Hpy188III TCNNGA 4 cut(s) 92, 175, 189, 203
Hpy8I GTNNAC 1 cut(s) 667
Hpy99I CGWCG 5 cut(s) 448, 482, 485, 639, 677
HpyAV CCTTC 2 cut(s) 370, 583
HpyCH4III ACNGT 2 cut(s) 639, 677
HpyCH4V TGCA 2 cut(s) 83, 108
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 80, 114, 134, 547
HpyF3I CTNAG 5 cut(s) 24, 323, 395, 699, 725
Hsp92I GRCGYC 3 cut(s) 128, 446, 616
Hsp92II CATG 1 cut(s) 112
HspAI GCGC 2 cut(s) 128, 250
KasI GGCGCC 1 cut(s) 127
Kzo9I GATC 4 cut(s) 171, 185, 258, 598
LmnI GCTCC 3 cut(s) 214, 605, 647
Lsp1109I GCAGC 4 cut(s) 58, 67, 95, 129
LweI GCATC 1 cut(s) 496
MaeI CTAG 2 cut(s) 299, 609
MaeIII GTNAC 1 cut(s) 435
MalI GATC 4 cut(s) 173, 187, 260, 600
MbiI CCGCTC 1 cut(s) 650
MboI GATC 4 cut(s) 171, 185, 258, 598
MflI RGATCY 1 cut(s) 258
MhlI GDGCHC 1 cut(s) 489
MluCI AATT 1 cut(s) 230
Mly113I GGCGCC 1 cut(s) 128
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 658
MseI TTAA 2 cut(s) 153, 419
MslI CAYNNNNRTG 1 cut(s) 464
MspI CCGG 6 cut(s) 75, 147, 256, 538, 555, 576
MspR9I CCNGG 6 cut(s) 76, 147, 277, 364, 499, 555
MvaI CCWGG 3 cut(s) 277, 364, 499
MwoI GCNNNNNNNGC 5 cut(s) 77, 80, 114, 134, 547
NarI GGCGCC 1 cut(s) 128
NciI CCSGG 3 cut(s) 76, 147, 555
NdeII GATC 4 cut(s) 171, 185, 258, 598
NlaIII CATG 1 cut(s) 112
NlaIV GGNNCC 3 cut(s) 129, 274, 342
NmuCI GTSAC 1 cut(s) 435
NsbI TGCGCA 1 cut(s) 251
PaeR7I CTCGAG 1 cut(s) 49
PceI AGGCCT 1 cut(s) 362
PfeI GAWTC 1 cut(s) 534
PflMI CCANNNNNTGG 1 cut(s) 174
PfoI TCCNGGA 1 cut(s) 553
PkrI GCNGC 6 cut(s) 73, 82, 85, 144, 496, 652
PleI GAGTC 1 cut(s) 41
PluTI GGCGCC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 41
PpuMI RGGWCCY 1 cut(s) 272
Psp5II RGGWCCY 1 cut(s) 272
Psp6I CCWGG 3 cut(s) 275, 362, 497
PspEI GGTNACC 1 cut(s) 435
PspGI CCWGG 3 cut(s) 275, 362, 497
PspN4I GGNNCC 3 cut(s) 129, 274, 342
PspPI GGNCC 3 cut(s) 115, 272, 631
PspPPI RGGWCCY 1 cut(s) 272
PspXI VCTCGAGB 1 cut(s) 49
PstI CTGCAG 1 cut(s) 85
PstNI CAGNNNCTG 1 cut(s) 245
PsuI RGATCY 1 cut(s) 258
RseI CAYNNNNRTG 1 cut(s) 464
SaqAI TTAA 2 cut(s) 153, 419
SatI GCNGC 6 cut(s) 72, 81, 84, 143, 495, 651
Sau3AI GATC 4 cut(s) 171, 185, 258, 598
Sau96I GGNCC 3 cut(s) 115, 272, 631
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 6 cut(s) 76, 147, 277, 364, 499, 555
SduI GDGCHC 1 cut(s) 489
SetI ASST 9 cut(s) 33, 247, 274, 342, 552, 610, 666, 700, 736
SfaNI GCATC 1 cut(s) 496
SfcI CTRYAG 2 cut(s) 38, 81
SfoI GGCGCC 1 cut(s) 129
Sfr274I CTCGAG 1 cut(s) 49
SinI GGWCC 2 cut(s) 272, 631
SlaI CTCGAG 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 1 cut(s) 49
SmoI CTYRAG 1 cut(s) 49
Sse9I AATT 1 cut(s) 230
SseBI AGGCCT 1 cut(s) 362
SsiI CCGC 4 cut(s) 464, 492, 495, 650
SspDI GGCGCC 1 cut(s) 127
SspMI CTAG 2 cut(s) 299, 609
StuI AGGCCT 1 cut(s) 362
StyD4I CCNGG 6 cut(s) 74, 145, 275, 362, 497, 553
TaaI ACNGT 2 cut(s) 639, 677
TaqI TCGA 4 cut(s) 50, 470, 504, 672
TasI AATT 1 cut(s) 230
TauI GCSGC 2 cut(s) 497, 653
TfiI GAWTC 1 cut(s) 534
Tru1I TTAA 2 cut(s) 153, 419
Tru9I TTAA 2 cut(s) 153, 419
TseFI GTSAC 1 cut(s) 435
TseI GCWGC 4 cut(s) 71, 80, 83, 142
Tsp45I GTSAC 1 cut(s) 435
TspGWI ACGGA 2 cut(s) 533, 660
Van91I CCANNNNNTGG 1 cut(s) 174
VpaK11BI GGWCC 2 cut(s) 272, 631
XhoI CTCGAG 1 cut(s) 49
XspI CTAG 2 cut(s) 299, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.