Rmu_ssc0000075.1_g000013

IMPACT family member in pol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000075.1
N/A
Physical Location & Seq
Reverse (-)
55258 .. 59758
4501 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1053 bp
atgcagtctgccaccattcttcacacccagcccaagatctcgcctacaccaccgcgcccaccactccacaatcaacgtcaggtctccaccaccaccgctctgtcgccgtcgtcagaccaccacaggaggacatcatccaactccactatcccatccccacgcagccgtgatccagacccgagaggaaacctgcccaaacaccgcccctctccatccctccacctacccgtgatagcaccgccgcctgtcccaccaccgcatcatggaacctcccaacgtcgatcccatccctcaccgacggatcataccctggtgcagccggatccgagaataaatggggaaagaaacctccacaccataacctccgaaacatcatctccggccatcacctcttgtcaccgccacctcctatcaacgcaacaccaaaatgcgacccaaacccaagagaaatcgaatccatacatcaaccccgacctctctgttccgctctccttctctctgccattgccgggaaacccaacttccggtcaccacctcacgtcaccaccaccatccttcgaaccagcccctaatttcaatcaaaacccgaaccctagcctcaaactccgaccaccggaaaacgccatacaagctcgccggaactcgaggttctgcatgtcttcattctccaagagccagttcgaatcgagcttcaacacttccataaacggagttcgaatggttgatggtggtgaagtgcaacttccacgatttagcctcaaacctagaaaagaagaaatggtaagggcctggtttggtgatcttgcaactgttgtggttgagttgggtgatttgagcatgttggtaagaatgggtgtagtggttccatttgatcttatcggcattctatatcatccgctacaatcttttcaagttgaagacatcaaacaagattatgagaccggtaaagatggtttcaccatggttacctttcaagttaattttgaccgtgttgagaaattagaggatgcgatcaaaaacaactgtagccgggagtcggtgttctatgagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0029

Protein Analysis

350

Amino Acids

39.02

Weight (kDa)

9.03

Isoelectric Point (pI)

59.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 198
AccBSI CCGCTC 2 cut(s) 98, 487
AccII CGCG 1 cut(s) 55
AciI CCGC 9 cut(s) 53, 96, 202, 239, 242, 257, 400, 485, 896
AclWI GGATC 5 cut(s) 164, 276, 309, 317, 330
AcoI YGGCCR 1 cut(s) 381
AfiI CCNNNNNNNGG 5 cut(s) 263, 509, 524, 613, 1036
AgeI ACCGGT 1 cut(s) 941
AgsI TTSAA 5 cut(s) 577, 694, 911, 917, 974
AjiI CACGTC 1 cut(s) 540
AjnI CCWGG 2 cut(s) 309, 788
AjuI GAANNNNNNNTTGG 2 cut(s) 662, 694
AloI GAACNNNNNNTCC 1 cut(s) 1025
AluBI AGCT 2 cut(s) 632, 690
AluI AGCT 2 cut(s) 632, 690
Alw26I GTCTC 2 cut(s) 88, 932
AlwI GGATC 5 cut(s) 164, 276, 309, 317, 330
Ama87I CYCGRG 2 cut(s) 178, 643
AoxI GGCC 2 cut(s) 381, 786
ApeKI GCWGC 2 cut(s) 162, 316
AsiGI ACCGGT 1 cut(s) 941
AspLEI GCGC 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 786
AsuC2I CCSGG 2 cut(s) 510, 1031
AsuHPI GGTGA 9 cut(s) 285, 379, 389, 521, 534, 743, 809, 839, 949
AsuII TTCGAA 3 cut(s) 558, 681, 715
AvaI CYCGRG 2 cut(s) 178, 643
BamHI GGATCC 1 cut(s) 322
BbsI GAAGAC 2 cut(s) 651, 924
BbvI GCAGC 2 cut(s) 174, 328
BccI CCATC 7 cut(s) 160, 220, 294, 392, 559, 719, 944
BceAI ACGGC 2 cut(s) 91, 150
BciT130I CCWGG 2 cut(s) 311, 790
BcnI CCSGG 2 cut(s) 510, 1031
BcoDI GTCTC 2 cut(s) 88, 932
BfaI CTAG 2 cut(s) 594, 765
BfmI CTRYAG 1 cut(s) 1024
BfuAI ACCTGC 1 cut(s) 198
BglII AGATCT 1 cut(s) 36
BisI GCNGC 3 cut(s) 163, 242, 317
BlsI GCNGC 3 cut(s) 164, 243, 318
Bme1390I CCNGG 4 cut(s) 311, 510, 790, 1031
BmeT110I CYCGRG 2 cut(s) 178, 643
BmgBI CACGTC 1 cut(s) 540
BmgT120I GGNCC 1 cut(s) 786
BmiI GGNNCC 3 cut(s) 268, 324, 864
BmrFI CCNGG 4 cut(s) 311, 510, 790, 1031
BmsI GCATC 2 cut(s) 268, 997
BpiI GAAGAC 2 cut(s) 651, 924
Bpu14I TTCGAA 3 cut(s) 558, 681, 715
BpuMI CCSGG 2 cut(s) 510, 1031
BsaI GGTCTC 2 cut(s) 88, 932
BsaJI CCNNGG 2 cut(s) 309, 960
BsaWI WCCGGW 3 cut(s) 524, 613, 941
BsaXI ACNNNNNCTCC 3 cut(s) 361, 391, 1025
Bsc4I CCNNNNNNNGG 5 cut(s) 263, 509, 524, 613, 1036
Bse118I RCCGGY 1 cut(s) 941
Bse1I ACTGG 1 cut(s) 676
Bse3DI GCAATG 1 cut(s) 503
BseBI CCWGG 2 cut(s) 311, 790
BseDI CCNNGG 2 cut(s) 309, 960
BseGI GGATG 7 cut(s) 134, 152, 212, 286, 551, 892, 1012
BseLI CCNNNNNNNGG 5 cut(s) 263, 509, 524, 613, 1036
BseMI GCAATG 1 cut(s) 503
BseNI ACTGG 1 cut(s) 676
BseXI GCAGC 2 cut(s) 174, 328
BseYI CCCAGC 1 cut(s) 27
BsgI GTGCAG 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 55
BshFI GGCC 2 cut(s) 383, 788
BshTI ACCGGT 1 cut(s) 941
BsiHKCI CYCGRG 2 cut(s) 178, 643
BsiSI CCGG 8 cut(s) 320, 380, 509, 525, 614, 637, 942, 1030
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 5 cut(s) 263, 509, 524, 613, 1036
BsmAI GTCTC 2 cut(s) 88, 932
BsmFI GGGAC 1 cut(s) 233
BsmI GAATGC 1 cut(s) 882
BsnI GGCC 2 cut(s) 383, 788
Bso31I GGTCTC 2 cut(s) 88, 932
BsoBI CYCGRG 2 cut(s) 178, 643
Bsp119I TTCGAA 3 cut(s) 558, 681, 715
Bsp143I GATC 8 cut(s) 36, 169, 281, 301, 322, 799, 871, 1011
Bsp19I CCATGG 1 cut(s) 960
BspACI CCGC 9 cut(s) 53, 96, 202, 239, 242, 257, 400, 485, 896
BspANI GGCC 2 cut(s) 383, 788
BspFNI CGCG 1 cut(s) 55
BspLI GGNNCC 3 cut(s) 268, 324, 864
BspMI ACCTGC 1 cut(s) 198
BspPI GGATC 5 cut(s) 164, 276, 309, 317, 330
BspT104I TTCGAA 3 cut(s) 558, 681, 715
BspTNI GGTCTC 2 cut(s) 88, 932
BsrBI CCGCTC 2 cut(s) 98, 487
BsrDI GCAATG 1 cut(s) 503
BsrFI RCCGGY 1 cut(s) 941
BsrI ACTGG 1 cut(s) 676
BssAI RCCGGY 1 cut(s) 941
BssECI CCNNGG 2 cut(s) 309, 960
BssMI GATC 8 cut(s) 36, 169, 281, 301, 322, 799, 871, 1011
BssT1I CCWWGG 1 cut(s) 960
Bst2UI CCWGG 2 cut(s) 311, 790
Bst4CI ACNGT 3 cut(s) 811, 989, 1025
BstBI TTCGAA 3 cut(s) 558, 681, 715
BstC8I GCNNGC 1 cut(s) 634
BstDSI CCRYGG 1 cut(s) 960
BstEII GGTNACC 2 cut(s) 527, 964
BstF5I GGATG 7 cut(s) 134, 152, 212, 286, 551, 892, 1012
BstFNI CGCG 1 cut(s) 55
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 8 cut(s) 39, 172, 284, 304, 325, 802, 874, 1014
BstMAI GTCTC 2 cut(s) 88, 932
BstMBI GATC 8 cut(s) 36, 169, 281, 301, 322, 799, 871, 1011
BstMWI GCNNNNNNNGC 1 cut(s) 629
BstNI CCWGG 2 cut(s) 311, 790
BstNSI RCATGY 2 cut(s) 658, 841
BstPI GGTNACC 2 cut(s) 527, 964
BstSCI CCNGG 4 cut(s) 309, 508, 788, 1029
BstSFI CTRYAG 1 cut(s) 1024
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 2 cut(s) 174, 328
BstV2I GAAGAC 2 cut(s) 651, 924
BstX2I RGATCY 2 cut(s) 36, 322
BstYI RGATCY 2 cut(s) 36, 322
BsuRI GGCC 2 cut(s) 383, 788
BtgI CCRYGG 1 cut(s) 960
BtrI CACGTC 1 cut(s) 540
BtsCI GGATG 7 cut(s) 134, 152, 212, 286, 551, 892, 1012
BveI ACCTGC 1 cut(s) 198
Cac8I GCNNGC 1 cut(s) 634
CfoI GCGC 1 cut(s) 57
Cfr10I RCCGGY 1 cut(s) 941
Cfr13I GGNCC 1 cut(s) 786
CspAI ACCGGT 1 cut(s) 941
CspCI CAANNNNNGTGG 2 cut(s) 795, 830
CviAII CATG 4 cut(s) 263, 655, 838, 961
DpnI GATC 8 cut(s) 38, 171, 283, 303, 324, 801, 873, 1013
DpnII GATC 8 cut(s) 36, 169, 281, 301, 322, 799, 871, 1011
EaeI YGGCCR 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 960
Eco31I GGTCTC 2 cut(s) 88, 932
Eco88I CYCGRG 2 cut(s) 178, 643
Eco91I GGTNACC 2 cut(s) 527, 964
EcoO109I RGGNCCY 1 cut(s) 786
EcoO65I GGTNACC 2 cut(s) 527, 964
EcoRII CCWGG 2 cut(s) 309, 788
EcoT14I CCWWGG 1 cut(s) 960
ErhI CCWWGG 1 cut(s) 960
FaeI CATG 4 cut(s) 266, 658, 841, 964
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 1 cut(s) 233
FatI CATG 4 cut(s) 262, 654, 837, 960
Fnu4HI GCNGC 3 cut(s) 163, 242, 317
FokI GGATG 7 cut(s) 121, 139, 199, 273, 538, 879, 1019
Fsp4HI GCNGC 3 cut(s) 163, 242, 317
FspBI CTAG 2 cut(s) 594, 765
GlaI GCGC 1 cut(s) 56
GluI GCNGC 3 cut(s) 163, 242, 317
GsaI CCCAGC 1 cut(s) 31
HaeIII GGCC 2 cut(s) 383, 788
HapII CCGG 8 cut(s) 320, 380, 509, 525, 614, 637, 942, 1030
HhaI GCGC 1 cut(s) 57
Hin1II CATG 4 cut(s) 266, 658, 841, 964
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HinfI GANTC 3 cut(s) 454, 683, 1034
HpaII CCGG 8 cut(s) 320, 380, 509, 525, 614, 637, 942, 1030
HphI GGTGA 9 cut(s) 285, 379, 389, 521, 534, 743, 809, 839, 949
Hpy188I TCNGA 4 cut(s) 115, 327, 367, 608
Hpy188III TCNNGA 1 cut(s) 173
Hpy99I CGWCG 3 cut(s) 112, 282, 301
HpyAV CCTTC 2 cut(s) 502, 565
HpyCH4III ACNGT 3 cut(s) 811, 989, 1025
HpyCH4IV ACGT 3 cut(s) 76, 277, 539
HpyCH4V TGCA 5 cut(s) 4, 316, 654, 739, 806
HpyF10VI GCNNNNNNNGC 1 cut(s) 629
HpySE526I ACGT 3 cut(s) 76, 277, 539
Hsp92II CATG 4 cut(s) 266, 658, 841, 964
HspAI GCGC 1 cut(s) 55
Kzo9I GATC 8 cut(s) 36, 169, 281, 301, 322, 799, 871, 1011
Lsp1109I GCAGC 2 cut(s) 174, 328
LweI GCATC 2 cut(s) 268, 997
MaeI CTAG 2 cut(s) 594, 765
MaeII ACGT 3 cut(s) 76, 277, 539
MaeIII GTNAC 4 cut(s) 395, 527, 540, 964
MalI GATC 8 cut(s) 38, 171, 283, 303, 324, 801, 873, 1013
MbiI CCGCTC 2 cut(s) 98, 487
MboI GATC 8 cut(s) 36, 169, 281, 301, 322, 799, 871, 1011
MboII GAAGA 4 cut(s) 11, 651, 785, 929
MflI RGATCY 2 cut(s) 36, 322
MluCI AATT 3 cut(s) 571, 979, 998
MlyI GAGTC 1 cut(s) 1043
MmeI TCCRAC 2 cut(s) 162, 631
MseI TTAA 1 cut(s) 978
MslI CAYNNNNRTG 1 cut(s) 426
MspI CCGG 8 cut(s) 320, 380, 509, 525, 614, 637, 942, 1030
MspR9I CCNGG 4 cut(s) 311, 510, 790, 1031
Mva1269I GAATGC 1 cut(s) 882
MvaI CCWGG 2 cut(s) 311, 790
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 1 cut(s) 629
NciI CCSGG 2 cut(s) 510, 1031
NcoI CCATGG 1 cut(s) 960
NdeII GATC 8 cut(s) 36, 169, 281, 301, 322, 799, 871, 1011
NlaIII CATG 4 cut(s) 266, 658, 841, 964
NlaIV GGNNCC 3 cut(s) 268, 324, 864
NmuCI GTSAC 3 cut(s) 395, 527, 540
NspI RCATGY 2 cut(s) 658, 841
NspV TTCGAA 3 cut(s) 558, 681, 715
PaeR7I CTCGAG 1 cut(s) 643
PctI GAATGC 1 cut(s) 882
PfeI GAWTC 2 cut(s) 454, 683
PinAI ACCGGT 1 cut(s) 941
PkrI GCNGC 3 cut(s) 164, 243, 318
PleI GAGTC 1 cut(s) 1042
PpsI GAGTC 1 cut(s) 1042
Psp6I CCWGG 2 cut(s) 309, 788
PspEI GGTNACC 2 cut(s) 527, 964
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 2 cut(s) 309, 788
PspN4I GGNNCC 3 cut(s) 268, 324, 864
PspPI GGNCC 1 cut(s) 786
PspXI VCTCGAGB 1 cut(s) 643
PsuI RGATCY 2 cut(s) 36, 322
RseI CAYNNNNRTG 1 cut(s) 426
SaqAI TTAA 1 cut(s) 978
SatI GCNGC 3 cut(s) 163, 242, 317
Sau3AI GATC 8 cut(s) 36, 169, 281, 301, 322, 799, 871, 1011
Sau96I GGNCC 1 cut(s) 786
SchI GAGTC 1 cut(s) 1043
ScrFI CCNGG 4 cut(s) 311, 510, 790, 1031
SfaNI GCATC 2 cut(s) 268, 997
SfcI CTRYAG 1 cut(s) 1024
Sfr274I CTCGAG 1 cut(s) 643
SfuI TTCGAA 3 cut(s) 558, 681, 715
SlaI CTCGAG 1 cut(s) 643
SmiMI CAYNNNNRTG 1 cut(s) 426
SmlI CTYRAG 1 cut(s) 643
SmoI CTYRAG 1 cut(s) 643
Sse9I AATT 3 cut(s) 571, 979, 998
SsiI CCGC 9 cut(s) 53, 96, 202, 239, 242, 257, 400, 485, 896
SspMI CTAG 2 cut(s) 594, 765
StyD4I CCNGG 4 cut(s) 309, 508, 788, 1029
StyI CCWWGG 1 cut(s) 960
TaaI ACNGT 3 cut(s) 811, 989, 1025
TaiI ACGT 3 cut(s) 79, 280, 542
TaqI TCGA 7 cut(s) 280, 452, 558, 644, 681, 686, 715
TasI AATT 3 cut(s) 571, 979, 998
TauI GCSGC 1 cut(s) 244
TfiI GAWTC 2 cut(s) 454, 683
Tru1I TTAA 1 cut(s) 978
Tru9I TTAA 1 cut(s) 978
TseFI GTSAC 3 cut(s) 395, 527, 540
TseI GCWGC 2 cut(s) 162, 316
Tsp45I GTSAC 3 cut(s) 395, 527, 540
TspDTI ATGAA 1 cut(s) 651
TspGWI ACGGA 2 cut(s) 314, 723
XceI RCATGY 2 cut(s) 658, 841
XhoI CTCGAG 1 cut(s) 643
XspI CTAG 2 cut(s) 594, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.