Rmu_ssc0000076.1_g000003

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000076.1
N/A
Physical Location & Seq
Reverse (-)
19358 .. 21121
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 828 bp
atgggggcggtgacgtcatccatggcggcgaagttcgcgttcttcccgccgcacccgccgtcgtacggggtggaggaggaggaggagagcgggaagctgaggatgacgggagtgccggcgagggcgaacgtcgacgttttgaagctgaagacgaagaggggaaacgacgtcgtcgcggtttactttaagaacccggcggcgaagctcaccgttctgcactcccacggtaacgcagctgatctgggccagatgtatgagctcttcagcgaggggcttcaatttctcgggacagctgatgatgttgtggattggtcacatggcaagcaactgtgggaacactgtaaggagaagtacgagcctttgtggataaaaggagggaaccattgtgacttggagctctacccacagtacatcaagcatctgaagaagtttgtatcggccattgagaagtttccacgtcttaaaaatggatctgcaccagttcttaaaaatggatctgcaccagttacggatcaactagaaaagccccgaggcagcaccgacatcagggagaaaccttggtccaacacagatcataaagaggtttccagaacaagtactgaccatagagaaaaccctagggtaagcacagactgcagagagaagtcaagggccagcactgataagagagatagatcaaaaaagattatggatcaacctgaaaagccaagtaatagcgtggagcagccggagaaagcaaggaatagcattgaccgctttggggagatgttcagatcagttggcttctgcaatattgattgttttaggcccacggcgaccagtgtatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4 Peptidase_S9

Protein Analysis

275

Amino Acids

30.98

Weight (kDa)

8.83

Isoelectric Point (pI)

32.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 17, 171
AccBSI CCGCTC 1 cut(s) 90
AccI GTMKAC 1 cut(s) 132
AccII CGCG 2 cut(s) 38, 176
AciI CCGC 9 cut(s) 8, 26, 47, 50, 56, 90, 176, 197, 754
AclWI GGATC 4 cut(s) 478, 502, 519, 699
AcoI YGGCCR 1 cut(s) 438
AcuI CTGAAG 3 cut(s) 167, 247, 443
AcyI GRCGYC 2 cut(s) 14, 168
AfaI GTAC 4 cut(s) 65, 353, 410, 598
AfiI CCNNNNNNNGG 3 cut(s) 65, 546, 760
AgsI TTSAA 2 cut(s) 142, 278
AjiI CACGTC 1 cut(s) 458
AluBI AGCT 7 cut(s) 97, 145, 205, 236, 259, 293, 397
AluI AGCT 7 cut(s) 97, 145, 205, 236, 259, 293, 397
Alw21I GWGCWC 2 cut(s) 261, 399
AlwI GGATC 4 cut(s) 478, 502, 519, 699
Ama87I CYCGRG 2 cut(s) 284, 528
AoxI GGCC 4 cut(s) 244, 438, 651, 806
ApeKI GCWGC 3 cut(s) 233, 534, 724
AspA2I CCTAGG 1 cut(s) 617
AspS9I GGNCC 4 cut(s) 244, 561, 651, 807
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 22, 199
AvaI CYCGRG 2 cut(s) 284, 528
AvaII GGWCC 1 cut(s) 561
AvrII CCTAGG 1 cut(s) 617
BanII GRGCYC 2 cut(s) 261, 399
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
BbsI GAAGAC 1 cut(s) 155
Bbv12I GWGCWC 2 cut(s) 261, 399
BbvCI CCTCAGC 1 cut(s) 98
BbvI GCAGC 3 cut(s) 245, 546, 736
BceAI ACGGC 1 cut(s) 43
BcnI CCSGG 1 cut(s) 194
BfaI CTAG 2 cut(s) 518, 618
BfmI CTRYAG 1 cut(s) 634
BisI GCNGC 6 cut(s) 27, 50, 198, 234, 535, 725
BlnI CCTAGG 1 cut(s) 617
BlsI GCNGC 6 cut(s) 28, 51, 199, 235, 536, 726
BmcAI AGTACT 1 cut(s) 598
Bme1390I CCNGG 1 cut(s) 194
Bme18I GGWCC 1 cut(s) 561
BmeT110I CYCGRG 2 cut(s) 284, 528
BmgBI CACGTC 1 cut(s) 458
BmgT120I GGNCC 4 cut(s) 244, 561, 651, 807
BmiI GGNNCC 1 cut(s) 380
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 1 cut(s) 427
BpiI GAAGAC 1 cut(s) 155
Bpu10I CCTNAGC 1 cut(s) 98
BpuMI CCSGG 1 cut(s) 194
BsaHI GRCGYC 2 cut(s) 14, 168
BsaJI CCNNGG 6 cut(s) 21, 223, 529, 557, 617, 810
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 546, 760
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 3 cut(s) 479, 503, 819
BseDI CCNNGG 6 cut(s) 21, 223, 529, 557, 617, 810
BseGI GGATG 2 cut(s) 17, 108
BseLI CCNNNNNNNGG 3 cut(s) 65, 546, 760
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 3 cut(s) 479, 503, 819
BseRI GAGGAG 4 cut(s) 89, 92, 95, 98
BseXI GCAGC 3 cut(s) 245, 546, 736
BsgI GTGCAG 3 cut(s) 200, 459, 483
Bsh1236I CGCG 2 cut(s) 38, 176
BshFI GGCC 4 cut(s) 246, 440, 653, 808
BsiHKAI GWGCWC 2 cut(s) 261, 399
BsiHKCI CYCGRG 2 cut(s) 284, 528
BsiSI CCGG 3 cut(s) 116, 194, 728
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 301
BslI CCNNNNNNNGG 3 cut(s) 65, 546, 760
BsmFI GGGAC 1 cut(s) 301
BsnI GGCC 4 cut(s) 246, 440, 653, 808
BsoBI CYCGRG 2 cut(s) 284, 528
Bsp1286I GDGCHC 2 cut(s) 261, 399
Bsp143I GATC 8 cut(s) 238, 470, 494, 511, 571, 674, 691, 773
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 9 cut(s) 8, 26, 47, 50, 56, 90, 176, 197, 754
BspANI GGCC 4 cut(s) 246, 440, 653, 808
BspCNI CTCAG 1 cut(s) 90
BspFNI CGCG 2 cut(s) 38, 176
BspLI GGNNCC 1 cut(s) 380
BspMAI CTGCAG 1 cut(s) 638
BspPI GGATC 4 cut(s) 478, 502, 519, 699
BspQI GCTCTTC 1 cut(s) 266
BsrBI CCGCTC 1 cut(s) 90
BsrFI RCCGGY 1 cut(s) 115
BsrI ACTGG 3 cut(s) 479, 503, 819
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 6 cut(s) 21, 223, 529, 557, 617, 810
BssMI GATC 8 cut(s) 238, 470, 494, 511, 571, 674, 691, 773
BssNI GRCGYC 2 cut(s) 14, 168
BssT1I CCWWGG 3 cut(s) 21, 557, 617
Bst4CI ACNGT 5 cut(s) 211, 227, 330, 341, 408
Bst6I CTCTTC 2 cut(s) 149, 266
BstACI GRCGYC 2 cut(s) 14, 168
BstAPI GCANNNNNTGC 1 cut(s) 633
BstC8I GCNNGC 3 cut(s) 117, 323, 655
BstDEI CTNAG 1 cut(s) 98
BstDSI CCRYGG 3 cut(s) 21, 223, 810
BstF5I GGATG 2 cut(s) 17, 108
BstFNI CGCG 2 cut(s) 38, 176
BstKTI GATC 8 cut(s) 241, 473, 497, 514, 574, 677, 694, 776
BstMBI GATC 8 cut(s) 238, 470, 494, 511, 571, 674, 691, 773
BstMWI GCNNNNNNNGC 4 cut(s) 35, 55, 633, 753
BstSCI CCNGG 1 cut(s) 192
BstSFI CTRYAG 1 cut(s) 634
BstUI CGCG 2 cut(s) 38, 176
BstV1I GCAGC 3 cut(s) 245, 546, 736
BstV2I GAAGAC 1 cut(s) 155
BstX2I RGATCY 2 cut(s) 470, 494
BstYI RGATCY 2 cut(s) 470, 494
BsuRI GGCC 4 cut(s) 246, 440, 653, 808
BtgI CCRYGG 3 cut(s) 21, 223, 810
BtrI CACGTC 1 cut(s) 458
BtsCI GGATG 2 cut(s) 17, 108
BtsIMutI CAGTG 3 cut(s) 337, 657, 826
Cac8I GCNNGC 3 cut(s) 117, 323, 655
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 4 cut(s) 244, 561, 651, 807
Csp6I GTAC 4 cut(s) 64, 352, 409, 597
CviAII CATG 2 cut(s) 22, 317
CviQI GTAC 4 cut(s) 64, 352, 409, 597
DdeI CTNAG 1 cut(s) 98
DpnI GATC 8 cut(s) 240, 472, 496, 513, 573, 676, 693, 775
DpnII GATC 8 cut(s) 238, 470, 494, 511, 571, 674, 691, 773
EaeI YGGCCR 1 cut(s) 438
Eam1104I CTCTTC 2 cut(s) 149, 266
EarI CTCTTC 2 cut(s) 149, 266
Ecl136II GAGCTC 2 cut(s) 259, 397
Eco130I CCWWGG 3 cut(s) 21, 557, 617
Eco24I GRGCYC 2 cut(s) 261, 399
Eco47I GGWCC 1 cut(s) 561
Eco53kI GAGCTC 2 cut(s) 259, 397
Eco57I CTGAAG 3 cut(s) 167, 247, 443
Eco88I CYCGRG 2 cut(s) 284, 528
EcoICRI GAGCTC 2 cut(s) 259, 397
EcoT14I CCWWGG 3 cut(s) 21, 557, 617
EcoT38I GRGCYC 2 cut(s) 261, 399
ErhI CCWWGG 3 cut(s) 21, 557, 617
FaeI CATG 2 cut(s) 25, 320
FaiI YATR 7 cut(s) 23, 255, 318, 576, 606, 689, 826
FaqI GGGAC 1 cut(s) 301
FatI CATG 2 cut(s) 21, 316
FauI CCCGC 3 cut(s) 54, 63, 83
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 6 cut(s) 27, 50, 198, 234, 535, 725
FokI GGATG 2 cut(s) 4, 115
FriOI GRGCYC 2 cut(s) 261, 399
Fsp4HI GCNGC 6 cut(s) 27, 50, 198, 234, 535, 725
FspBI CTAG 2 cut(s) 518, 618
GluI GCNGC 6 cut(s) 27, 50, 198, 234, 535, 725
HaeIII GGCC 4 cut(s) 246, 440, 653, 808
HapII CCGG 3 cut(s) 116, 194, 728
Hin1I GRCGYC 2 cut(s) 14, 168
Hin1II CATG 2 cut(s) 25, 320
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HpaII CCGG 3 cut(s) 116, 194, 728
HphI GGTGA 2 cut(s) 22, 199
Hpy166II GTNNAC 2 cut(s) 133, 181
Hpy188I TCNGA 2 cut(s) 423, 773
Hpy188III TCNNGA 2 cut(s) 286, 588
Hpy8I GTNNAC 2 cut(s) 133, 181
Hpy99I CGWCG 6 cut(s) 64, 134, 137, 170, 173, 176
HpyCH4III ACNGT 5 cut(s) 211, 227, 330, 341, 408
HpyCH4IV ACGT 5 cut(s) 14, 129, 135, 168, 457
HpyCH4V TGCA 5 cut(s) 217, 476, 500, 636, 789
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 55, 633, 753
HpyF3I CTNAG 1 cut(s) 98
HpySE526I ACGT 5 cut(s) 14, 129, 135, 168, 457
Hsp92I GRCGYC 2 cut(s) 14, 168
Hsp92II CATG 2 cut(s) 25, 320
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
Kzo9I GATC 8 cut(s) 238, 470, 494, 511, 571, 674, 691, 773
LguI GCTCTTC 1 cut(s) 266
LmnI GCTCC 2 cut(s) 394, 721
Lsp1109I GCAGC 3 cut(s) 245, 546, 736
LweI GCATC 1 cut(s) 427
MaeI CTAG 2 cut(s) 518, 618
MaeII ACGT 5 cut(s) 14, 129, 135, 168, 457
MaeIII GTNAC 5 cut(s) 10, 227, 312, 386, 505
MalI GATC 8 cut(s) 240, 472, 496, 513, 573, 676, 693, 775
MbiI CCGCTC 1 cut(s) 90
MboI GATC 8 cut(s) 238, 470, 494, 511, 571, 674, 691, 773
MboII GAAGA 5 cut(s) 34, 160, 166, 253, 436
MflI RGATCY 2 cut(s) 470, 494
MhlI GDGCHC 2 cut(s) 261, 399
MluCI AATT 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 588
MroNI GCCGGC 1 cut(s) 115
MseI TTAA 3 cut(s) 186, 462, 486
MspA1I CMGCKG 2 cut(s) 236, 293
MspI CCGG 3 cut(s) 116, 194, 728
MspR9I CCNGG 1 cut(s) 194
MvnI CGCG 2 cut(s) 38, 176
MwoI GCNNNNNNNGC 4 cut(s) 35, 55, 633, 753
NaeI GCCGGC 1 cut(s) 117
NciI CCSGG 1 cut(s) 194
NcoI CCATGG 1 cut(s) 21
NdeII GATC 8 cut(s) 238, 470, 494, 511, 571, 674, 691, 773
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 2 cut(s) 25, 320
NlaIV GGNNCC 1 cut(s) 380
NmuCI GTSAC 3 cut(s) 10, 312, 386
PciSI GCTCTTC 1 cut(s) 266
PdiI GCCGGC 1 cut(s) 117
Pfl23II CGTACG 1 cut(s) 63
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 6 cut(s) 28, 51, 199, 235, 536, 726
Psp124BI GAGCTC 2 cut(s) 261, 399
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 380
PspPI GGNCC 4 cut(s) 244, 561, 651, 807
PstI CTGCAG 1 cut(s) 638
PsuI RGATCY 2 cut(s) 470, 494
PsyI GACNNNGTC 1 cut(s) 170
PvuII CAGCTG 2 cut(s) 236, 293
RsaI GTAC 4 cut(s) 65, 353, 410, 598
RsaNI GTAC 4 cut(s) 64, 352, 409, 597
SacI GAGCTC 2 cut(s) 261, 399
SalI GTCGAC 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 266
SaqAI TTAA 3 cut(s) 186, 462, 486
SatI GCNGC 6 cut(s) 27, 50, 198, 234, 535, 725
Sau3AI GATC 8 cut(s) 238, 470, 494, 511, 571, 674, 691, 773
Sau96I GGNCC 4 cut(s) 244, 561, 651, 807
ScaI AGTACT 1 cut(s) 598
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 2 cut(s) 261, 399
SfaNI GCATC 1 cut(s) 427
SfcI CTRYAG 1 cut(s) 634
SgrDI CGTCGACG 1 cut(s) 131
SinI GGWCC 1 cut(s) 561
Sse9I AATT 1 cut(s) 278
SsiI CCGC 9 cut(s) 8, 26, 47, 50, 56, 90, 176, 197, 754
SspI AATATT 1 cut(s) 793
SspMI CTAG 2 cut(s) 518, 618
SstI GAGCTC 2 cut(s) 261, 399
StyD4I CCNGG 1 cut(s) 192
StyI CCWWGG 3 cut(s) 21, 557, 617
TaaI ACNGT 5 cut(s) 211, 227, 330, 341, 408
TaiI ACGT 5 cut(s) 17, 132, 138, 171, 460
TaqI TCGA 1 cut(s) 132
TasI AATT 1 cut(s) 278
TatI WGTACW 2 cut(s) 408, 596
TauI GCSGC 3 cut(s) 29, 52, 200
Tru1I TTAA 3 cut(s) 186, 462, 486
Tru9I TTAA 3 cut(s) 186, 462, 486
TscAI CASTG 3 cut(s) 344, 664, 826
TseFI GTSAC 3 cut(s) 10, 312, 386
TseI GCWGC 3 cut(s) 233, 534, 724
Tsp45I GTSAC 3 cut(s) 10, 312, 386
TspGWI ACGGA 1 cut(s) 524
TspRI CASTG 3 cut(s) 344, 664, 826
Tth111I GACNNNGTC 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 561
XmaJI CCTAGG 1 cut(s) 617
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 2 cut(s) 518, 618
ZraI GACGTC 2 cut(s) 15, 169
ZrmI AGTACT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.