Rmu_ssc0000136.1_g000004

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000136.1
N/A
Physical Location & Seq
Reverse (-)
5612 .. 7896
2285 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 552 bp
atgtgcacggccgttgccacagtcacctcatcctctgactcagcttaccccacagtcaccgaggactgctcggtgggctccggcgaggccgtccctgtcccggtacgtagagaggtgtacgataacgcgacttcttgtggatttgtccactcgggcactcacgtcgacgcatctggtcacgtcttcgaccgttactaccatgctggatttgatgttgatgctttagacctcgaagtgttaaacggccccgcgctggtggttgatgttcccagggataagaacatgaccggaattgattacttgaacgtcgcagctttcgttgatgtagttccagtgcataatacttttctggaaggcaggcaaatggttccagatttcaaattagacactgatacagacttacaagcacataaaggagaccatcatttgataagaatgcatctgattggtgatccttctgcacctgtcagcctcaaaggtaagcaatggacagaatatattcctggttggatgtcatcaagttttagaccttctacaagaatgatctcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

183

Amino Acids

19.95

Weight (kDa)

5.04

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 48 - 104 7.1e-07 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 165
AccII CGCG 2 cut(s) 128, 251
AciI CCGC 1 cut(s) 249
AclWI GGATC 1 cut(s) 446
AcoI YGGCCR 1 cut(s) 9
AfaI GTAC 2 cut(s) 105, 119
AfiI CCNNNNNNNGG 2 cut(s) 100, 253
AgsI TTSAA 2 cut(s) 304, 379
AjiI CACGTC 2 cut(s) 163, 181
AjnI CCWGG 2 cut(s) 269, 502
AluBI AGCT 2 cut(s) 44, 314
AluI AGCT 2 cut(s) 44, 314
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 411
Alw44I GTGCAC 1 cut(s) 4
AlwI GGATC 1 cut(s) 446
Ama87I CYCGRG 1 cut(s) 151
AoxI GGCC 3 cut(s) 9, 87, 244
ApaLI GTGCAC 1 cut(s) 4
ApeKI GCWGC 1 cut(s) 311
Asp700I GAANNNNTTC 1 cut(s) 498
AspLEI GCGC 1 cut(s) 253
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 3 cut(s) 16, 49, 461
AvaI CYCGRG 1 cut(s) 151
BaeGI GKGCMC 2 cut(s) 8, 158
BanII GRGCYC 1 cut(s) 80
BbsI GAAGAC 1 cut(s) 175
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 1 cut(s) 323
BccI CCATC 1 cut(s) 429
BceAI ACGGC 3 cut(s) 24, 74, 259
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BciT130I CCWGG 2 cut(s) 271, 504
BcnI CCSGG 1 cut(s) 101
BcoDI GTCTC 1 cut(s) 411
BisI GCNGC 1 cut(s) 312
BlsI GCNGC 1 cut(s) 313
Bme1390I CCNGG 3 cut(s) 101, 271, 504
BmeT110I CYCGRG 1 cut(s) 151
BmgBI CACGTC 2 cut(s) 163, 181
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 3 cut(s) 79, 247, 369
BmrFI CCNGG 3 cut(s) 101, 271, 504
BmsI GCATC 3 cut(s) 179, 208, 448
BpiI GAAGAC 1 cut(s) 175
BplI GAGNNNNNCTC 2 cut(s) 53, 85
BpuMI CCSGG 1 cut(s) 101
BsaAI YACGTR 1 cut(s) 107
BsaI GGTCTC 1 cut(s) 411
BsaJI CCNNGG 3 cut(s) 60, 269, 270
BsaWI WCCGGW 1 cut(s) 287
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 253
Bse1I ACTGG 1 cut(s) 332
Bse3DI GCAATG 1 cut(s) 491
BseBI CCWGG 2 cut(s) 271, 504
BseDI CCNNGG 3 cut(s) 60, 269, 270
BseGI GGATG 2 cut(s) 29, 516
BseLI CCNNNNNNNGG 2 cut(s) 100, 253
BseMI GCAATG 1 cut(s) 491
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 1 cut(s) 332
BseSI GKGCMC 2 cut(s) 8, 158
BseX3I CGGCCG 1 cut(s) 9
BseXI GCAGC 1 cut(s) 323
BsgI GTGCAG 1 cut(s) 444
Bsh1236I CGCG 2 cut(s) 128, 251
Bsh1285I CGRYCG 2 cut(s) 12, 190
BshFI GGCC 3 cut(s) 11, 89, 246
BsiEI CGRYCG 2 cut(s) 12, 190
BsiHKAI GWGCWC 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 3 cut(s) 81, 101, 288
BslFI GGGAC 2 cut(s) 77, 83
BslI CCNNNNNNNGG 2 cut(s) 100, 253
BsmAI GTCTC 1 cut(s) 411
BsmFI GGGAC 2 cut(s) 77, 83
BsmI GAATGC 1 cut(s) 441
BsnI GGCC 3 cut(s) 11, 89, 246
Bso31I GGTCTC 1 cut(s) 411
BsoBI CYCGRG 1 cut(s) 151
Bsp1286I GDGCHC 3 cut(s) 8, 80, 158
Bsp143I GATC 2 cut(s) 451, 543
BspACI CCGC 1 cut(s) 249
BspANI GGCC 3 cut(s) 11, 89, 246
BspCNI CTCAG 1 cut(s) 53
BspFNI CGCG 2 cut(s) 128, 251
BspLI GGNNCC 3 cut(s) 79, 247, 369
BspPI GGATC 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 411
BsrDI GCAATG 1 cut(s) 491
BsrI ACTGG 1 cut(s) 332
BssECI CCNNGG 3 cut(s) 60, 269, 270
BssMI GATC 2 cut(s) 451, 543
Bst2UI CCWGG 2 cut(s) 271, 504
Bst4CI ACNGT 3 cut(s) 22, 55, 191
BstBAI YACGTR 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 1 cut(s) 40
BstF5I GGATG 2 cut(s) 29, 516
BstFNI CGCG 2 cut(s) 128, 251
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 2 cut(s) 454, 546
BstMAI GTCTC 1 cut(s) 411
BstMBI GATC 2 cut(s) 451, 543
BstMCI CGRYCG 2 cut(s) 12, 190
BstMWI GCNNNNNNNGC 1 cut(s) 75
BstNI CCWGG 2 cut(s) 271, 504
BstSCI CCNGG 3 cut(s) 99, 269, 502
BstSLI GKGCMC 2 cut(s) 8, 158
BstSNI TACGTA 1 cut(s) 107
BstUI CGCG 2 cut(s) 128, 251
BstV1I GCAGC 1 cut(s) 323
BstV2I GAAGAC 1 cut(s) 175
BstZI CGGCCG 1 cut(s) 9
BsuRI GGCC 3 cut(s) 11, 89, 246
BtrI CACGTC 2 cut(s) 163, 181
BtsCI GGATG 2 cut(s) 29, 516
BtsIMutI CAGTG 2 cut(s) 339, 387
Cac8I GCNNGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 245
CseI GACGC 1 cut(s) 176
Csp6I GTAC 2 cut(s) 104, 118
CviAII CATG 2 cut(s) 200, 283
CviJI RGCY 7 cut(s) 11, 44, 78, 89, 246, 314, 471
CviKI_1 RGCY 7 cut(s) 11, 44, 78, 89, 246, 314, 471
CviQI GTAC 2 cut(s) 104, 118
DdeI CTNAG 1 cut(s) 40
DpnI GATC 2 cut(s) 453, 545
DpnII GATC 2 cut(s) 451, 543
EaeI YGGCCR 1 cut(s) 9
EagI CGGCCG 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 9
Eco105I TACGTA 1 cut(s) 107
Eco24I GRGCYC 1 cut(s) 80
Eco31I GGTCTC 1 cut(s) 411
Eco52I CGGCCG 1 cut(s) 9
Eco88I CYCGRG 1 cut(s) 151
EcoRII CCWGG 2 cut(s) 269, 502
EcoT22I ATGCAT 1 cut(s) 441
EcoT38I GRGCYC 1 cut(s) 80
FaeI CATG 2 cut(s) 203, 286
FaiI YATR 5 cut(s) 201, 284, 339, 411, 498
FaqI GGGAC 2 cut(s) 77, 83
FatI CATG 2 cut(s) 199, 282
FauI CCCGC 1 cut(s) 256
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 2 cut(s) 16, 523
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 1 cut(s) 312
GlaI GCGC 1 cut(s) 252
GluI GCNGC 1 cut(s) 312
HaeIII GGCC 3 cut(s) 11, 89, 246
HapII CCGG 3 cut(s) 81, 101, 288
HgaI GACGC 1 cut(s) 176
HhaI GCGC 1 cut(s) 253
Hin1II CATG 2 cut(s) 203, 286
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 1 cut(s) 38
HpaII CCGG 3 cut(s) 81, 101, 288
HphI GGTGA 3 cut(s) 16, 49, 461
Hpy166II GTNNAC 4 cut(s) 6, 118, 148, 166
Hpy188I TCNGA 2 cut(s) 37, 444
Hpy188III TCNNGA 3 cut(s) 350, 371, 549
Hpy8I GTNNAC 4 cut(s) 6, 118, 148, 166
Hpy99I CGWCG 3 cut(s) 167, 170, 311
HpyAV CCTTC 3 cut(s) 347, 465, 540
HpyCH4III ACNGT 3 cut(s) 22, 55, 191
HpyCH4IV ACGT 4 cut(s) 106, 162, 180, 306
HpyCH4V TGCA 4 cut(s) 6, 337, 439, 461
HpyF10VI GCNNNNNNNGC 1 cut(s) 75
HpyF3I CTNAG 1 cut(s) 40
HpySE526I ACGT 4 cut(s) 106, 162, 180, 306
Hsp92II CATG 2 cut(s) 203, 286
HspAI GCGC 1 cut(s) 251
Kzo9I GATC 2 cut(s) 451, 543
LmnI GCTCC 1 cut(s) 83
Lsp1109I GCAGC 1 cut(s) 323
LweI GCATC 3 cut(s) 179, 208, 448
MaeII ACGT 4 cut(s) 106, 162, 180, 306
MaeIII GTNAC 4 cut(s) 22, 55, 176, 191
MalI GATC 2 cut(s) 453, 545
MboI GATC 2 cut(s) 451, 543
MboII GAAGA 1 cut(s) 175
MhlI GDGCHC 3 cut(s) 8, 80, 158
MluCI AATT 2 cut(s) 291, 380
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 488
MnlI CCTC 7 cut(s) 37, 43, 55, 79, 106, 239, 482
Mph1103I ATGCAT 1 cut(s) 441
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 1 cut(s) 239
MspI CCGG 3 cut(s) 81, 101, 288
MspR9I CCNGG 3 cut(s) 101, 271, 504
Mva1269I GAATGC 1 cut(s) 441
MvaI CCWGG 2 cut(s) 271, 504
MvnI CGCG 2 cut(s) 128, 251
MwoI GCNNNNNNNGC 1 cut(s) 75
NciI CCSGG 1 cut(s) 101
NdeII GATC 2 cut(s) 451, 543
NlaIII CATG 2 cut(s) 203, 286
NlaIV GGNNCC 3 cut(s) 79, 247, 369
NmuCI GTSAC 3 cut(s) 22, 55, 176
NsiI ATGCAT 1 cut(s) 441
PasI CCCWGGG 1 cut(s) 270
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 498
PkrI GCNGC 1 cut(s) 313
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
Ppu21I YACGTR 1 cut(s) 107
Psp6I CCWGG 2 cut(s) 269, 502
PspGI CCWGG 2 cut(s) 269, 502
PspN4I GGNNCC 3 cut(s) 79, 247, 369
PspPI GGNCC 1 cut(s) 245
RsaI GTAC 2 cut(s) 105, 119
RsaNI GTAC 2 cut(s) 104, 118
SalI GTCGAC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 239
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 2 cut(s) 451, 543
Sau96I GGNCC 1 cut(s) 245
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 3 cut(s) 101, 271, 504
SduI GDGCHC 3 cut(s) 8, 80, 158
SfaNI GCATC 3 cut(s) 179, 208, 448
SgrDI CGTCGACG 1 cut(s) 164
SnaBI TACGTA 1 cut(s) 107
Sse9I AATT 2 cut(s) 291, 380
SsiI CCGC 1 cut(s) 249
StyD4I CCNGG 3 cut(s) 99, 269, 502
TaaI ACNGT 3 cut(s) 22, 55, 191
TaiI ACGT 4 cut(s) 109, 165, 183, 309
TaqI TCGA 3 cut(s) 165, 186, 231
TasI AATT 2 cut(s) 291, 380
Tru1I TTAA 1 cut(s) 239
Tru9I TTAA 1 cut(s) 239
TscAI CASTG 2 cut(s) 339, 394
TseFI GTSAC 3 cut(s) 22, 55, 176
TseI GCWGC 1 cut(s) 311
Tsp45I GTSAC 3 cut(s) 22, 55, 176
TspRI CASTG 2 cut(s) 339, 394
VneI GTGCAC 1 cut(s) 4
XmiI GTMKAC 1 cut(s) 165
XmnI GAANNNNTTC 1 cut(s) 498
Zsp2I ATGCAT 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.