Rmu_ssc0000148.1_g000010

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000148.1
N/A
Physical Location & Seq
Reverse (-)
50239 .. 51385
1147 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 612 bp
atggcttggtggcaaggtttcgatgaaactcgtcttctcatagcacctgatcctcctgtagccggagacggcgccgggcaactgatttcgctccgccacccaaaatccgaacaagccctggaacgtatacatgctacctatcaagatattcatgatgatggtggtgatctatacaatgttgctcttcgttttcgactcctcagacaacatggatatagtgtttcctgcgatgtattcaacaagttcaaagatgctaacggtgacttcaaggaaagcttagttactgatgtttcaggtatgctaagcttctatgaggcatcccatcaaatgatgcatggagaaaaattacttgaagaggctctcgattttaccaccactcatctcaagactgcaacaaatttaaccagtgcaagctcattactgaaggcacaaataactgaagccctagagagacctctactaaaaactatagagaggttaggtgctcggcgttacatgtcaatataccaagatgaagcttcacacaacgaggagttactgaaacttgcaaagttagattttaatgttgtacagtgtttacacaaaaaggaactcagtgacattatgaggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

203

Amino Acids

23.05

Weight (kDa)

5.7

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 37 - 149 4.2e-39 Terpene synthase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccI GTMKAC 1 cut(s) 127
AciI CCGC 1 cut(s) 94
AclWI GGATC 1 cut(s) 44
AcsI RAATTY 1 cut(s) 397
AcuI CTGAAG 2 cut(s) 443, 459
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 1 cut(s) 62
AflIII ACRYGT 1 cut(s) 495
AgsI TTSAA 4 cut(s) 238, 247, 268, 353
AjnI CCWGG 1 cut(s) 117
AluBI AGCT 4 cut(s) 276, 306, 414, 518
AluI AGCT 4 cut(s) 276, 306, 414, 518
Alw21I GWGCWC 1 cut(s) 487
Alw26I GTCTC 2 cut(s) 60, 445
AlwI GGATC 1 cut(s) 44
ApoI RAATTY 1 cut(s) 397
AspLEI GCGC 1 cut(s) 74
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 176, 272
BanI GGYRCC 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 26
Bbv12I GWGCWC 1 cut(s) 487
BccI CCATC 2 cut(s) 152, 330
BceAI ACGGC 1 cut(s) 85
BciT130I CCWGG 1 cut(s) 119
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 2 cut(s) 60, 445
BfaI CTAG 1 cut(s) 446
BfmI CTRYAG 2 cut(s) 57, 468
BfoI RGCGCY 1 cut(s) 75
BlpI GCTNAGC 1 cut(s) 302
Bme1390I CCNGG 2 cut(s) 76, 119
BmiI GGNNCC 1 cut(s) 73
BmrFI CCNGG 2 cut(s) 76, 119
BmsI GCATC 3 cut(s) 241, 321, 326
BpiI GAAGAC 1 cut(s) 26
Bpu1102I GCTNAGC 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 1 cut(s) 76
BsaHI GRCGYC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 445
BsaJI CCNNGG 1 cut(s) 117
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 405
BseBI CCWGG 1 cut(s) 119
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 1 cut(s) 317
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMII CTCAG 2 cut(s) 214, 607
BseNI ACTGG 1 cut(s) 405
BseRI GAGGAG 2 cut(s) 188, 545
BshNI GGYRCC 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 487
BsiSI CCGG 2 cut(s) 63, 75
BslI CCNNNNNNNGG 1 cut(s) 62
BsmAI GTCTC 2 cut(s) 60, 445
BsmBI CGTCTC 1 cut(s) 60
Bso31I GGTCTC 1 cut(s) 445
Bsp1286I GDGCHC 1 cut(s) 487
Bsp1407I TGTACA 1 cut(s) 568
Bsp143I GATC 2 cut(s) 49, 166
Bsp1720I GCTNAGC 1 cut(s) 302
BspACI CCGC 1 cut(s) 94
BspCNI CTCAG 2 cut(s) 213, 606
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 73
BspPI GGATC 1 cut(s) 44
BspQI GCTCTTC 1 cut(s) 189
BspT107I GGYRCC 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 445
BsrGI TGTACA 1 cut(s) 568
BsrI ACTGG 1 cut(s) 405
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 2 cut(s) 49, 166
BssNAI GTATAC 1 cut(s) 128
BssNI GRCGYC 1 cut(s) 72
Bst1107I GTATAC 1 cut(s) 128
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 2 cut(s) 260, 573
Bst6I CTCTTC 2 cut(s) 189, 348
BstACI GRCGYC 1 cut(s) 72
BstAUI TGTACA 1 cut(s) 568
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 4 cut(s) 200, 277, 302, 593
BstF5I GGATG 1 cut(s) 317
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 2 cut(s) 52, 169
BstMAI GTCTC 2 cut(s) 60, 445
BstMBI GATC 2 cut(s) 49, 166
BstNI CCWGG 1 cut(s) 119
BstNSI RCATGY 2 cut(s) 134, 499
BstSCI CCNGG 2 cut(s) 74, 117
BstSFI CTRYAG 2 cut(s) 57, 468
BstV2I GAAGAC 1 cut(s) 26
BstZ17I GTATAC 1 cut(s) 128
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 1 cut(s) 317
BtsIMutI CAGTG 3 cut(s) 412, 578, 601
Cac8I GCNNGC 1 cut(s) 412
CciI TCATGA 1 cut(s) 151
CfoI GCGC 1 cut(s) 74
Csp6I GTAC 1 cut(s) 569
CviAII CATG 5 cut(s) 131, 152, 209, 335, 496
CviJI RGCY 9 cut(s) 5, 62, 116, 276, 306, 359, 414, 443, 518
CviKI_1 RGCY 9 cut(s) 5, 62, 116, 276, 306, 359, 414, 443, 518
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 4 cut(s) 200, 277, 302, 593
DinI GGCGCC 1 cut(s) 73
DpnI GATC 2 cut(s) 51, 168
DpnII GATC 2 cut(s) 49, 166
Eam1104I CTCTTC 2 cut(s) 189, 348
EarI CTCTTC 2 cut(s) 189, 348
EciI GGCGGA 1 cut(s) 83
Eco31I GGTCTC 1 cut(s) 445
Eco57I CTGAAG 2 cut(s) 443, 459
EcoRII CCWGG 1 cut(s) 117
EcoT22I ATGCAT 1 cut(s) 336
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
Esp3I CGTCTC 1 cut(s) 60
FaeI CATG 5 cut(s) 134, 155, 212, 338, 499
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FatI CATG 5 cut(s) 130, 151, 208, 334, 495
FblI GTMKAC 1 cut(s) 127
FokI GGATG 1 cut(s) 304
FspBI CTAG 1 cut(s) 446
GlaI GCGC 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 75
HapII CCGG 2 cut(s) 63, 75
HhaI GCGC 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 5 cut(s) 134, 155, 212, 338, 499
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HindIII AAGCTT 3 cut(s) 274, 304, 516
HinfI GANTC 1 cut(s) 195
HpaII CCGG 2 cut(s) 63, 75
HphI GGTGA 2 cut(s) 176, 272
Hpy166II GTNNAC 2 cut(s) 128, 578
Hpy188I TCNGA 2 cut(s) 109, 203
Hpy188III TCNNGA 4 cut(s) 143, 152, 362, 385
Hpy8I GTNNAC 2 cut(s) 128, 578
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 2 cut(s) 260, 573
HpyCH4IV ACGT 1 cut(s) 124
HpyCH4V TGCA 4 cut(s) 334, 392, 410, 548
HpyF3I CTNAG 4 cut(s) 200, 277, 302, 593
HpySE526I ACGT 1 cut(s) 124
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 5 cut(s) 134, 155, 212, 338, 499
HspAI GCGC 1 cut(s) 72
KasI GGCGCC 1 cut(s) 71
Kzo9I GATC 2 cut(s) 49, 166
LguI GCTCTTC 1 cut(s) 189
LmnI GCTCC 1 cut(s) 96
LpnPI CCDG 9 cut(s) 60, 69, 76, 88, 104, 131, 238, 279, 418
LweI GCATC 3 cut(s) 241, 321, 326
MaeI CTAG 1 cut(s) 446
MaeII ACGT 1 cut(s) 124
MaeIII GTNAC 5 cut(s) 260, 280, 491, 534, 596
MalI GATC 2 cut(s) 51, 168
MboI GATC 2 cut(s) 49, 166
MboII GAAGA 3 cut(s) 26, 176, 365
MhlI GDGCHC 1 cut(s) 487
MluCI AATT 2 cut(s) 344, 397
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 1 cut(s) 189
MnlI CCTC 8 cut(s) 63, 209, 307, 349, 465, 468, 523, 600
Mph1103I ATGCAT 1 cut(s) 336
MseI TTAA 2 cut(s) 401, 561
MslI CAYNNNNRTG 1 cut(s) 156
MspI CCGG 2 cut(s) 63, 75
MspR9I CCNGG 2 cut(s) 76, 119
MvaI CCWGG 1 cut(s) 119
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 1 cut(s) 76
NdeII GATC 2 cut(s) 49, 166
NlaIII CATG 5 cut(s) 134, 155, 212, 338, 499
NlaIV GGNNCC 1 cut(s) 73
NmeAIII GCCGAG 1 cut(s) 466
NmuCI GTSAC 2 cut(s) 260, 596
NsiI ATGCAT 1 cut(s) 336
NspI RCATGY 2 cut(s) 134, 499
PagI TCATGA 1 cut(s) 151
PciI ACATGT 1 cut(s) 495
PciSI GCTCTTC 1 cut(s) 189
PleI GAGTC 1 cut(s) 189
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 189
PscI ACATGT 1 cut(s) 495
Psp6I CCWGG 1 cut(s) 117
PspGI CCWGG 1 cut(s) 117
PspN4I GGNNCC 1 cut(s) 73
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
RseI CAYNNNNRTG 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 189
SaqAI TTAA 2 cut(s) 401, 561
Sau3AI GATC 2 cut(s) 49, 166
SchI GAGTC 1 cut(s) 189
ScrFI CCNGG 2 cut(s) 76, 119
SduI GDGCHC 1 cut(s) 487
SfaNI GCATC 3 cut(s) 241, 321, 326
SfcI CTRYAG 2 cut(s) 57, 468
SfoI GGCGCC 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 156
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 2 cut(s) 344, 397
SsiI CCGC 1 cut(s) 94
SspDI GGCGCC 1 cut(s) 71
SspMI CTAG 1 cut(s) 446
StyD4I CCNGG 2 cut(s) 74, 117
TaaI ACNGT 2 cut(s) 260, 573
TaiI ACGT 1 cut(s) 127
TaqI TCGA 3 cut(s) 21, 193, 363
TasI AATT 2 cut(s) 344, 397
TatI WGTACW 1 cut(s) 568
Tru1I TTAA 2 cut(s) 401, 561
Tru9I TTAA 2 cut(s) 401, 561
TscAI CASTG 3 cut(s) 412, 578, 601
TseFI GTSAC 2 cut(s) 260, 596
Tsp45I GTSAC 2 cut(s) 260, 596
TspDTI ATGAA 3 cut(s) 39, 140, 528
TspRI CASTG 3 cut(s) 412, 578, 601
XapI RAATTY 1 cut(s) 397
XceI RCATGY 2 cut(s) 134, 499
XmiI GTMKAC 1 cut(s) 127
XspI CTAG 1 cut(s) 446
Zsp2I ATGCAT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.