Rmu_ssc0000218.1_g000025

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000218.1
N/A
Physical Location & Seq
Forward (+)
108176 .. 108935
760 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 681 bp
atgcataagctcgatttttctaaactagattcaaatgtcacaagatatcacagttgggcggctgatgtcgaaaatcacttcactgccaatggaattttgcccaatattcaagcaccaaatgttgacttgctgtttcagcctactacggctaagagtgcccaagcccttatattgatgaggcgacatatggatgagtcactccaaagggagtatatgtcgatcaaggaccctagggctttatggattgtgcttgaagagttctttggagatatccaagatatcctcctcctccttgacttgaagatgcagtggaatgatctaagcttcactgacttcaagacagtttctgaatataatttagaagctctacgaatcaagctttcagcattattggtttcaaagcaatattggcttgagtacaatgctcaacggatcacgaggtttcatcagcttgtcactgctatgtcagtcgtagctaagagcgttcatgaggtgaattatggtgacacacctaaaggagggcgcaaggagcagaactctgaagttaagggacatcaaactagatgtgcgagtccatacaacagccctacaaatgaaggaaatcaccataacgatgagcagacatgggagaggcagggtcgacactaccgaccgtggtggagctgccaacaacgtccatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

226

Amino Acids

26.46

Weight (kDa)

6.8

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 640
AciI CCGC 1 cut(s) 59
AclWI GGATC 1 cut(s) 440
AcsI RAATTY 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 561
AfaI GTAC 1 cut(s) 419
AfiI CCNNNNNNNGG 1 cut(s) 518
AgsI TTSAA 6 cut(s) 33, 110, 254, 301, 337, 399
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AluBI AGCT 7 cut(s) 10, 324, 365, 379, 451, 476, 663
AluI AGCT 7 cut(s) 10, 324, 365, 379, 451, 476, 663
AlwI GGATC 1 cut(s) 440
AlwNI CAGNNNCTG 1 cut(s) 347
ApeKI GCWGC 1 cut(s) 663
ApoI RAATTY 1 cut(s) 93
AspA2I CCTAGG 1 cut(s) 230
AspLEI GCGC 1 cut(s) 525
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 3 cut(s) 505, 515, 596
AvaII GGWCC 1 cut(s) 226
AvrII CCTAGG 1 cut(s) 230
BaeGI GKGCMC 1 cut(s) 160
BauI CACGAG 1 cut(s) 436
BbvI GCAGC 1 cut(s) 650
BceAI ACGGC 1 cut(s) 162
BfaI CTAG 3 cut(s) 26, 231, 561
BisI GCNGC 2 cut(s) 60, 664
BlnI CCTAGG 1 cut(s) 230
BlsI GCNGC 2 cut(s) 61, 665
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 1 cut(s) 228
BmsI GCATC 1 cut(s) 294
BplI GAGNNNNNCTC 2 cut(s) 521, 553
BpuEI CTTGAG 1 cut(s) 434
BsaJI CCNNGG 2 cut(s) 230, 653
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bsc4I CCNNNNNNNGG 1 cut(s) 518
BseDI CCNNGG 2 cut(s) 230, 653
BseGI GGATG 1 cut(s) 196
BseLI CCNNNNNNNGG 1 cut(s) 518
BseRI GAGGAG 2 cut(s) 275, 278
BseSI GKGCMC 1 cut(s) 160
BseXI GCAGC 1 cut(s) 650
Bsh1285I CGRYCG 1 cut(s) 653
BsiEI CGRYCG 1 cut(s) 653
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 1 cut(s) 518
BsmFI GGGAC 1 cut(s) 564
Bsp1286I GDGCHC 1 cut(s) 160
Bsp143I GATC 3 cut(s) 219, 316, 432
BspACI CCGC 1 cut(s) 59
BspHI TCATGA 1 cut(s) 487
BspLI GGNNCC 1 cut(s) 228
BspPI GGATC 1 cut(s) 440
BssECI CCNNGG 2 cut(s) 230, 653
BssMI GATC 3 cut(s) 219, 316, 432
BssSI CACGAG 1 cut(s) 436
BssT1I CCWWGG 1 cut(s) 230
Bst2BI CACGAG 1 cut(s) 436
Bst4CI ACNGT 3 cut(s) 53, 343, 654
Bst6I CTCTTC 1 cut(s) 249
BstDEI CTNAG 3 cut(s) 150, 320, 477
BstDSI CCRYGG 1 cut(s) 653
BstENI CCTNNNNNAGG 1 cut(s) 516
BstF5I GGATG 1 cut(s) 196
BstHHI GCGC 1 cut(s) 525
BstKTI GATC 3 cut(s) 222, 319, 435
BstMBI GATC 3 cut(s) 219, 316, 432
BstMCI CGRYCG 1 cut(s) 653
BstMWI GCNNNNNNNGC 4 cut(s) 136, 155, 409, 529
BstSLI GKGCMC 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 650
BtgI CCRYGG 1 cut(s) 653
BtsCI GGATG 1 cut(s) 196
BtsI GCAGTG 3 cut(s) 81, 314, 456
BtsIMutI CAGTG 4 cut(s) 81, 314, 327, 456
CaiI CAGNNNCTG 1 cut(s) 347
CciI TCATGA 1 cut(s) 487
CfoI GCGC 1 cut(s) 525
Cfr13I GGNCC 1 cut(s) 226
Csp6I GTAC 1 cut(s) 418
CviAII CATG 2 cut(s) 488, 624
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 3 cut(s) 150, 320, 477
DpnI GATC 3 cut(s) 221, 318, 434
DpnII GATC 3 cut(s) 219, 316, 432
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 230
Eco32I GATATC 3 cut(s) 47, 271, 280
Eco47I GGWCC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 561
EcoNI CCTNNNNNAGG 1 cut(s) 516
EcoO109I RGGNCCY 1 cut(s) 226
EcoRV GATATC 3 cut(s) 47, 271, 280
EcoT14I CCWWGG 1 cut(s) 230
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 230
FaeI CATG 2 cut(s) 491, 627
FaqI GGGAC 1 cut(s) 564
FatI CATG 2 cut(s) 487, 623
FauNDI CATATG 1 cut(s) 186
FblI GTMKAC 1 cut(s) 640
Fnu4HI GCNGC 2 cut(s) 60, 664
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 2 cut(s) 60, 664
FspBI CTAG 3 cut(s) 26, 231, 561
GlaI GCGC 1 cut(s) 524
GluI GCNGC 2 cut(s) 60, 664
HhaI GCGC 1 cut(s) 525
Hin1II CATG 2 cut(s) 491, 627
Hin6I GCGC 1 cut(s) 523
HinP1I GCGC 1 cut(s) 523
HincII GTYRAC 2 cut(s) 124, 641
HindII GTYRAC 2 cut(s) 124, 641
HindIII AAGCTT 2 cut(s) 322, 377
HinfI GANTC 4 cut(s) 29, 194, 372, 571
HphI GGTGA 3 cut(s) 505, 515, 596
Hpy166II GTNNAC 2 cut(s) 124, 641
Hpy188I TCNGA 2 cut(s) 349, 541
Hpy188III TCNNGA 3 cut(s) 337, 436, 488
Hpy8I GTNNAC 2 cut(s) 124, 641
HpyAV CCTTC 1 cut(s) 590
HpyCH4III ACNGT 3 cut(s) 53, 343, 654
HpyCH4IV ACGT 1 cut(s) 673
HpyCH4V TGCA 2 cut(s) 4, 307
HpyF10VI GCNNNNNNNGC 4 cut(s) 136, 155, 409, 529
HpyF3I CTNAG 3 cut(s) 150, 320, 477
HpySE526I ACGT 1 cut(s) 673
Hsp92II CATG 2 cut(s) 491, 627
HspAI GCGC 1 cut(s) 523
Kzo9I GATC 3 cut(s) 219, 316, 432
LmnI GCTCC 2 cut(s) 529, 660
LpnPI CCDG 1 cut(s) 620
Lsp1109I GCAGC 1 cut(s) 650
LweI GCATC 1 cut(s) 294
MaeI CTAG 3 cut(s) 26, 231, 561
MaeII ACGT 1 cut(s) 673
MaeIII GTNAC 4 cut(s) 37, 195, 454, 503
MalI GATC 3 cut(s) 221, 318, 434
MboI GATC 3 cut(s) 219, 316, 432
MboII GAAGA 2 cut(s) 266, 313
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 3 cut(s) 93, 355, 496
MlyI GAGTC 2 cut(s) 203, 580
MnlI CCTC 8 cut(s) 171, 293, 296, 299, 432, 484, 512, 624
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 546
MslI CAYNNNNRTG 3 cut(s) 189, 461, 612
MwoI GCNNNNNNNGC 4 cut(s) 136, 155, 409, 529
NdeI CATATG 1 cut(s) 186
NdeII GATC 3 cut(s) 219, 316, 432
NlaIII CATG 2 cut(s) 491, 627
NlaIV GGNNCC 1 cut(s) 228
NmuCI GTSAC 4 cut(s) 37, 195, 454, 503
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 646
PfeI GAWTC 2 cut(s) 29, 372
PkrI GCNGC 2 cut(s) 61, 665
PleI GAGTC 2 cut(s) 202, 579
PpsI GAGTC 2 cut(s) 202, 579
PpuMI RGGWCCY 1 cut(s) 226
Psp5II RGGWCCY 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 1 cut(s) 226
PspPPI RGGWCCY 1 cut(s) 226
PstNI CAGNNNCTG 1 cut(s) 347
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
RseI CAYNNNNRTG 3 cut(s) 189, 461, 612
SalI GTCGAC 1 cut(s) 639
SaqAI TTAA 1 cut(s) 546
SatI GCNGC 2 cut(s) 60, 664
Sau3AI GATC 3 cut(s) 219, 316, 432
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 2 cut(s) 203, 580
SduI GDGCHC 1 cut(s) 160
SfaNI GCATC 1 cut(s) 294
SinI GGWCC 1 cut(s) 226
SmiMI CAYNNNNRTG 3 cut(s) 189, 461, 612
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse9I AATT 3 cut(s) 93, 355, 496
SsiI CCGC 1 cut(s) 59
SspI AATATT 2 cut(s) 106, 407
SspMI CTAG 3 cut(s) 26, 231, 561
StyI CCWWGG 1 cut(s) 230
TaaI ACNGT 3 cut(s) 53, 343, 654
TaiI ACGT 1 cut(s) 676
TaqI TCGA 4 cut(s) 12, 69, 218, 640
TasI AATT 3 cut(s) 93, 355, 496
TatI WGTACW 1 cut(s) 417
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 2 cut(s) 29, 372
Tru1I TTAA 1 cut(s) 546
Tru9I TTAA 1 cut(s) 546
TscAI CASTG 4 cut(s) 88, 314, 334, 463
TseFI GTSAC 4 cut(s) 37, 195, 454, 503
TseI GCWGC 1 cut(s) 663
Tsp45I GTSAC 4 cut(s) 37, 195, 454, 503
TspDTI ATGAA 3 cut(s) 434, 476, 609
TspGWI ACGGA 1 cut(s) 445
TspRI CASTG 4 cut(s) 88, 314, 334, 463
VpaK11BI GGWCC 1 cut(s) 226
XagI CCTNNNNNAGG 1 cut(s) 516
XapI RAATTY 1 cut(s) 93
XmaJI CCTAGG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 640
XspI CTAG 3 cut(s) 26, 231, 561
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.