Rmu_ssc0000226.1_g000028

histone H2B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000226.1
N/A
Physical Location & Seq
Forward (+)
98548 .. 99051
504 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 504 bp
atggcgcccaaagccgagaagaagcccgcggagaagaagccggccgagaagaccacggtggccgagaaagccccggcggagaagaagcccaaggccggaaagaagctcccgaaggaagccggagccaccgccggtgacaagaagaagaagagggccaaaaagagcgtggagacctacaagatctacatcttcaaggtagacaagaagaagaagagggccaagaagagcgtggagacctacaagatctacatcttcaaggtcttgaagcaggtccaccctgacatcggaatctccagcaaggccatgggcatcatgaacagcttcatcaacgacatcttcgagaagctcgctcaggagtcgtcgaggctcgccaggtacaacaagaagccaacgattacttcccgggagatccagacggcggtgaggcttgtgcttcccggtgaattggctaagcatgctgtttctgaggggactaaggcggtgaccaagttcacaagctcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

167

Amino Acids

18.58

Weight (kDa)

10.16

Isoelectric Point (pI)

21.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 67 - 143 8e-20 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 97 - 165 6.2e-08 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 198
AccII CGCG 1 cut(s) 29
AciI CCGC 6 cut(s) 27, 29, 77, 129, 419, 479
AclWI GGATC 1 cut(s) 403
AcoI YGGCCR 2 cut(s) 42, 60
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 377
AfiI CCNNNNNNNGG 3 cut(s) 95, 284, 418
AgsI TTSAA 3 cut(s) 193, 256, 265
AjnI CCWGG 1 cut(s) 371
AjuI GAANNNNNNNTTGG 2 cut(s) 382, 414
AluBI AGCT 4 cut(s) 106, 321, 346, 498
AluI AGCT 4 cut(s) 106, 321, 346, 498
Alw26I GTCTC 2 cut(s) 164, 227
AlwI GGATC 1 cut(s) 403
Ama87I CYCGRG 1 cut(s) 402
AoxI GGCC 6 cut(s) 42, 60, 93, 153, 216, 300
Asp700I GAANNNNTTC 1 cut(s) 320
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 3 cut(s) 153, 216, 271
AsuC2I CCSGG 4 cut(s) 74, 403, 404, 438
AsuHPI GGTGA 4 cut(s) 146, 433, 452, 493
AvaI CYCGRG 1 cut(s) 402
AvaII GGWCC 1 cut(s) 271
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 56
BceAI ACGGC 1 cut(s) 432
BciT130I CCWGG 1 cut(s) 373
BcnI CCSGG 4 cut(s) 74, 403, 404, 438
BcoDI GTCTC 2 cut(s) 164, 227
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 259
BglII AGATCT 2 cut(s) 180, 243
BlpI GCTNAGC 1 cut(s) 450
Bme1390I CCNGG 5 cut(s) 74, 373, 403, 404, 438
Bme18I GGWCC 1 cut(s) 271
BmeT110I CYCGRG 1 cut(s) 402
BmgT120I GGNCC 3 cut(s) 153, 216, 271
BmiI GGNNCC 2 cut(s) 6, 124
BmrFI CCNGG 5 cut(s) 74, 373, 403, 404, 438
BmsI GCATC 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 56
BpmI CTGGAG 1 cut(s) 277
Bpu10I CCTNAGC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 450
BpuMI CCSGG 4 cut(s) 74, 403, 404, 438
BsaBI GATNNNNATC 2 cut(s) 185, 248
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 164, 227
BsaJI CCNNGG 6 cut(s) 27, 54, 72, 90, 303, 402
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 284, 418
Bse118I RCCGGY 2 cut(s) 40, 131
Bse8I GATNNNNATC 2 cut(s) 185, 248
BseBI CCWGG 1 cut(s) 373
BseDI CCNNGG 6 cut(s) 27, 54, 72, 90, 303, 402
BseJI GATNNNNATC 2 cut(s) 185, 248
BseLI CCNNNNNNNGG 3 cut(s) 95, 284, 418
BseMII CTCAG 2 cut(s) 365, 456
BseX3I CGGCCG 1 cut(s) 42
Bsh1236I CGCG 1 cut(s) 29
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 6 cut(s) 44, 62, 95, 155, 218, 302
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 402
BsiSI CCGG 7 cut(s) 41, 74, 96, 120, 132, 403, 438
BslFI GGGAC 1 cut(s) 484
BslI CCNNNNNNNGG 3 cut(s) 95, 284, 418
BsmAI GTCTC 2 cut(s) 164, 227
BsmFI GGGAC 1 cut(s) 484
BsnI GGCC 6 cut(s) 44, 62, 95, 155, 218, 302
Bso31I GGTCTC 2 cut(s) 164, 227
BsoBI CYCGRG 1 cut(s) 402
Bsp143I GATC 3 cut(s) 180, 243, 408
Bsp1720I GCTNAGC 1 cut(s) 450
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 6 cut(s) 27, 29, 77, 129, 419, 479
BspANI GGCC 6 cut(s) 44, 62, 95, 155, 218, 302
BspCNI CTCAG 2 cut(s) 364, 457
BspFNI CGCG 1 cut(s) 29
BspHI TCATGA 1 cut(s) 312
BspLI GGNNCC 2 cut(s) 6, 124
BspMI ACCTGC 1 cut(s) 259
BspPI GGATC 1 cut(s) 403
BspQI GCTCTTC 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 2 cut(s) 164, 227
BsrFI RCCGGY 2 cut(s) 40, 131
BssAI RCCGGY 2 cut(s) 40, 131
BssECI CCNNGG 6 cut(s) 27, 54, 72, 90, 303, 402
BssMI GATC 3 cut(s) 180, 243, 408
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 90, 303
Bst2UI CCWGG 1 cut(s) 373
Bst4CI ACNGT 1 cut(s) 58
Bst6I CTCTTC 3 cut(s) 143, 206, 218
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 5 cut(s) 27, 42, 348, 369, 456
BstDEI CTNAG 4 cut(s) 351, 450, 465, 474
BstDSI CCRYGG 3 cut(s) 27, 54, 303
BstEII GGTNACC 1 cut(s) 481
BstFNI CGCG 1 cut(s) 29
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 3 cut(s) 183, 246, 411
BstMAI GTCTC 2 cut(s) 164, 227
BstMBI GATC 3 cut(s) 180, 243, 408
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 3 cut(s) 11, 68, 455
BstNI CCWGG 1 cut(s) 373
BstNSI RCATGY 1 cut(s) 458
BstPI GGTNACC 1 cut(s) 481
BstSCI CCNGG 5 cut(s) 72, 371, 401, 402, 436
BstUI CGCG 1 cut(s) 29
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 3 cut(s) 180, 243, 408
BstYI RGATCY 3 cut(s) 180, 243, 408
BstZI CGGCCG 1 cut(s) 42
BsuRI GGCC 6 cut(s) 44, 62, 95, 155, 218, 302
BtgI CCRYGG 3 cut(s) 27, 54, 303
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 5 cut(s) 27, 42, 348, 369, 456
CciI TCATGA 1 cut(s) 312
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 2 cut(s) 40, 131
Cfr13I GGNCC 3 cut(s) 153, 216, 271
Cfr42I CCGCGG 1 cut(s) 30
Cfr9I CCCGGG 1 cut(s) 402
Csp6I GTAC 1 cut(s) 376
CviAII CATG 3 cut(s) 304, 313, 455
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 4 cut(s) 351, 450, 465, 474
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 182, 245, 410
DpnII GATC 3 cut(s) 180, 243, 408
EaeI YGGCCR 2 cut(s) 42, 60
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 3 cut(s) 143, 206, 218
EarI CTCTTC 3 cut(s) 143, 206, 218
EciI GGCGGA 1 cut(s) 92
EclXI CGGCCG 1 cut(s) 42
Eco130I CCWWGG 2 cut(s) 90, 303
Eco31I GGTCTC 2 cut(s) 164, 227
Eco47I GGWCC 1 cut(s) 271
Eco52I CGGCCG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 402
Eco91I GGTNACC 1 cut(s) 481
EcoO65I GGTNACC 1 cut(s) 481
EcoRII CCWGG 1 cut(s) 371
EcoT14I CCWWGG 2 cut(s) 90, 303
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 90, 303
FaeI CATG 3 cut(s) 307, 316, 458
FaiI YATR 3 cut(s) 305, 314, 456
FaqI GGGAC 1 cut(s) 484
FatI CATG 3 cut(s) 303, 312, 454
FauI CCCGC 1 cut(s) 34
FblI GTMKAC 1 cut(s) 198
GlaI GCGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 277
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 6 cut(s) 44, 62, 95, 155, 218, 302
HapII CCGG 7 cut(s) 41, 74, 96, 120, 132, 403, 438
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 307, 316, 458
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 2 cut(s) 288, 356
HpaII CCGG 7 cut(s) 41, 74, 96, 120, 132, 403, 438
HphI GGTGA 4 cut(s) 146, 433, 452, 493
Hpy166II GTNNAC 3 cut(s) 199, 274, 492
Hpy188I TCNGA 2 cut(s) 287, 466
Hpy188III TCNNGA 7 cut(s) 109, 262, 313, 340, 353, 412, 501
Hpy8I GTNNAC 3 cut(s) 199, 274, 492
Hpy99I CGWCG 1 cut(s) 364
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 68, 455
HpyF3I CTNAG 4 cut(s) 351, 450, 465, 474
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 307, 316, 458
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
KspI CCGCGG 1 cut(s) 30
Kzo9I GATC 3 cut(s) 180, 243, 408
LguI GCTCTTC 1 cut(s) 218
LmnI GCTCC 2 cut(s) 111, 122
LweI GCATC 1 cut(s) 318
MaeIII GTNAC 2 cut(s) 134, 481
MalI GATC 3 cut(s) 182, 245, 410
MboI GATC 3 cut(s) 180, 243, 408
MflI RGATCY 3 cut(s) 180, 243, 408
MluCI AATT 1 cut(s) 443
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 365
MnlI CCTC 5 cut(s) 144, 207, 357, 417, 460
MroNI GCCGGC 1 cut(s) 40
MroXI GAANNNNTTC 1 cut(s) 320
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 7 cut(s) 41, 74, 96, 120, 132, 403, 438
MspR9I CCNGG 5 cut(s) 74, 373, 403, 404, 438
MvaI CCWGG 1 cut(s) 373
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 3 cut(s) 11, 68, 455
NaeI GCCGGC 1 cut(s) 42
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 4 cut(s) 74, 403, 404, 438
NcoI CCATGG 1 cut(s) 303
NdeII GATC 3 cut(s) 180, 243, 408
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 3 cut(s) 307, 316, 458
NlaIV GGNNCC 2 cut(s) 6, 124
NmeAIII GCCGAG 3 cut(s) 40, 70, 88
NmuCI GTSAC 2 cut(s) 134, 481
NspI RCATGY 1 cut(s) 458
PaeI GCATGC 1 cut(s) 458
PagI TCATGA 1 cut(s) 312
PciSI GCTCTTC 1 cut(s) 218
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PdiI GCCGGC 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 320
PfeI GAWTC 1 cut(s) 288
PleI GAGTC 1 cut(s) 364
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 364
Psp6I CCWGG 1 cut(s) 371
PspEI GGTNACC 1 cut(s) 481
PspGI CCWGG 1 cut(s) 371
PspN4I GGNNCC 2 cut(s) 6, 124
PspPI GGNCC 3 cut(s) 153, 216, 271
PsuI RGATCY 3 cut(s) 180, 243, 408
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
SacII CCGCGG 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 218
Sau3AI GATC 3 cut(s) 180, 243, 408
Sau96I GGNCC 3 cut(s) 153, 216, 271
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 5 cut(s) 74, 373, 403, 404, 438
SfaNI GCATC 1 cut(s) 318
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 30
SgrAI CRCCGGYG 1 cut(s) 131
SgrBI CCGCGG 1 cut(s) 30
SinI GGWCC 1 cut(s) 271
SmaI CCCGGG 1 cut(s) 404
SphI GCATGC 1 cut(s) 458
Sse9I AATT 1 cut(s) 443
SsiI CCGC 6 cut(s) 27, 29, 77, 129, 419, 479
SspDI GGCGCC 1 cut(s) 4
StyD4I CCNGG 5 cut(s) 72, 371, 401, 402, 436
StyI CCWWGG 2 cut(s) 90, 303
TaaI ACNGT 1 cut(s) 58
TaqI TCGA 2 cut(s) 339, 362
TasI AATT 1 cut(s) 443
TfiI GAWTC 1 cut(s) 288
TseFI GTSAC 2 cut(s) 134, 481
Tsp45I GTSAC 2 cut(s) 134, 481
TspDTI ATGAA 2 cut(s) 313, 329
TspMI CCCGGG 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 271
XceI RCATGY 1 cut(s) 458
XcmI CCANNNNNNNNNTGG 3 cut(s) 163, 226, 301
XmaI CCCGGG 1 cut(s) 402
XmiI GTMKAC 1 cut(s) 198
XmnI GAANNNNTTC 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.