Rmu_ssc0000248.1_g000006

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000248.1
N/A
Physical Location & Seq
Reverse (-)
19033 .. 22674
3642 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 993 bp
atggaacgactgggagtcgggtcgaaattattgggcgccgtgccagataggtgggtcgttttggactgtttatgtgagagagaaaatcggatacgcagcaatctcccaacgaacccaactggctattgggccgatgtaaccggcgggcccaaatttaaattggagtctcgcgcatttggactaccacatgacagtgctacaccggaaccacataataattacccttcaaatggactccttggcactaggtatgtgaaagtccaaaacttaactgttcatgcttctcctgcatctccatatactagcggaatagtcccagattcttctgagcatgtttgcattgagaactgcaacattagtatgggtcatgatgcaattgttcttaagagcggttgggatgagtatggaatcgcctatggcaaaccgaccaaaagtgtacacatccgggccgtcaaccttcaatcttctttaggctgcggcctggcatttgggagtgagatgtctggaggcattttaaacgtactagtagagcggcttcatctgtctaactcattcgttggcattgaactcaagacaaccagaggaagaggaggatatatgaaagacattgttatatcgaatgtagaaatgcacaacattcacacagcaattcaggtcactggtcagagtggctctcatcctgatgataagtttgaccctgatcaagttccatttgttaaaggaatcacctttgacaacatggtcggcagtaatattacatttgctgggaatttttctggaatctacgaatcacctttcacatctatttgtctctcaaatgtgtcattctccatgactggggattcttctagctcatggttctgttccgatgtgaaggggttttcccagaaggtttctccagaaccatgttcagacctcaaggaatcatcttttaatgcttcctcatcttgcttcttttccttatacccttacaattatgctacagatttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

330

Amino Acids

35.81

Weight (kDa)

5.79

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 85 - 292 8.5e-17 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 740
AccB1I GGYRCC 1 cut(s) 35
AccBSI CCGCTC 2 cut(s) 390, 532
AccII CGCG 1 cut(s) 171
AciI CCGC 5 cut(s) 144, 306, 390, 477, 532
AcsI RAATTY 2 cut(s) 152, 769
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 2 cut(s) 438, 522
AfiI CCNNNNNNNGG 2 cut(s) 230, 837
AflII CTTAAG 1 cut(s) 383
AgsI TTSAA 3 cut(s) 228, 461, 566
AhdI GACNNNNNGTC 1 cut(s) 14
AhlI ACTAGT 1 cut(s) 523
AjnI CCWGG 1 cut(s) 480
AluBI AGCT 1 cut(s) 852
AluI AGCT 1 cut(s) 852
Alw26I GTCTC 2 cut(s) 171, 815
AoxI GGCC 4 cut(s) 129, 146, 447, 478
ApaI GGGCCC 1 cut(s) 150
ApeKI GCWGC 2 cut(s) 96, 474
ApoI RAATTY 2 cut(s) 152, 769
AspLEI GCGC 2 cut(s) 38, 173
AspS9I GGNCC 4 cut(s) 129, 146, 147, 447
AsuC2I CCSGG 1 cut(s) 446
AsuHPI GGTGA 2 cut(s) 718, 783
BaeGI GKGCMC 1 cut(s) 150
BanI GGYRCC 1 cut(s) 35
BanII GRGCYC 1 cut(s) 150
BarI GAAGNNNNNNTAC 2 cut(s) 519, 551
BbvI GCAGC 2 cut(s) 108, 461
BceAI ACGGC 2 cut(s) 23, 434
BciT130I CCWGG 1 cut(s) 482
BciVI GTATCC 1 cut(s) 84
BclI TGATCA 1 cut(s) 700
BcnI CCSGG 1 cut(s) 446
BcoDI GTCTC 2 cut(s) 171, 815
BcuI ACTAGT 1 cut(s) 523
BfaI CTAG 4 cut(s) 246, 303, 524, 849
BfmI CTRYAG 1 cut(s) 981
BfoI RGCGCY 1 cut(s) 39
BfrI CTTAAG 1 cut(s) 383
BfuI GTATCC 1 cut(s) 84
BisI GCNGC 4 cut(s) 97, 475, 478, 533
BlsI GCNGC 4 cut(s) 98, 476, 479, 534
Bme1390I CCNGG 2 cut(s) 446, 482
BmeRI GACNNNNNGTC 1 cut(s) 14
BmgT120I GGNCC 4 cut(s) 129, 146, 147, 447
BmiI GGNNCC 3 cut(s) 37, 148, 207
BmrFI CCNGG 2 cut(s) 446, 482
BmrI ACTGGG 2 cut(s) 20, 846
BmsI GCATC 2 cut(s) 299, 361
BmuI ACTGGG 2 cut(s) 20, 846
BplI GAGNNNNNCTC 2 cut(s) 658, 690
BpmI CTGGAG 2 cut(s) 525, 882
BpuEI CTTGAG 2 cut(s) 554, 902
BpuMI CCSGG 1 cut(s) 446
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 238
BsaWI WCCGGW 1 cut(s) 202
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 837
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 4 cut(s) 15, 124, 664, 841
BseBI CCWGG 1 cut(s) 482
BseDI CCNNGG 1 cut(s) 238
BseGI GGATG 3 cut(s) 403, 441, 676
BseLI CCNNNNNNNGG 2 cut(s) 230, 837
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 4 cut(s) 15, 124, 664, 841
BseRI GAGGAG 1 cut(s) 603
BseSI GKGCMC 1 cut(s) 150
BseXI GCAGC 2 cut(s) 108, 461
BseYI CCCAGC 1 cut(s) 764
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 4 cut(s) 131, 148, 449, 480
BshNI GGYRCC 1 cut(s) 35
BsiSI CCGG 3 cut(s) 141, 203, 445
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 2 cut(s) 230, 837
BsmAI GTCTC 2 cut(s) 171, 815
BsmFI GGGAC 1 cut(s) 299
BsnI GGCC 4 cut(s) 131, 148, 449, 480
Bsp120I GGGCCC 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 150
Bsp1407I TGTACA 1 cut(s) 436
Bsp143I GATC 1 cut(s) 700
BspACI CCGC 5 cut(s) 144, 306, 390, 477, 532
BspANI GGCC 4 cut(s) 131, 148, 449, 480
BspCNI CTCAG 1 cut(s) 319
BspFNI CGCG 1 cut(s) 171
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 3 cut(s) 37, 148, 207
BspT107I GGYRCC 1 cut(s) 35
BspTI CTTAAG 1 cut(s) 383
BsrBI CCGCTC 2 cut(s) 390, 532
BsrFI RCCGGY 1 cut(s) 140
BsrGI TGTACA 1 cut(s) 436
BsrI ACTGG 4 cut(s) 15, 124, 664, 841
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 1 cut(s) 238
BssMI GATC 1 cut(s) 700
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 238
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 3 cut(s) 68, 194, 274
Bst6I CTCTTC 1 cut(s) 580
BstACI GRCGYC 1 cut(s) 36
BstAFI CTTAAG 1 cut(s) 383
BstAUI TGTACA 1 cut(s) 436
BstC8I GCNNGC 1 cut(s) 146
BstDEI CTNAG 1 cut(s) 327
BstF5I GGATG 3 cut(s) 403, 441, 676
BstFNI CGCG 1 cut(s) 171
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 2 cut(s) 38, 173
BstKTI GATC 1 cut(s) 703
BstMAI GTCTC 2 cut(s) 171, 815
BstMBI GATC 1 cut(s) 700
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstNI CCWGG 1 cut(s) 482
BstNSI RCATGY 1 cut(s) 335
BstSCI CCNGG 2 cut(s) 444, 480
BstSFI CTRYAG 1 cut(s) 981
BstSLI GKGCMC 1 cut(s) 150
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 108, 461
BstXI CCANNNNNNTGG 1 cut(s) 51
BsuI GTATCC 1 cut(s) 84
BsuRI GGCC 4 cut(s) 131, 148, 449, 480
BtsCI GGATG 3 cut(s) 403, 441, 676
BtsIMutI CAGTG 2 cut(s) 199, 657
Cac8I GCNNGC 1 cut(s) 146
CciI TCATGA 1 cut(s) 367
CfoI GCGC 2 cut(s) 38, 173
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 4 cut(s) 129, 146, 147, 447
Csp6I GTAC 2 cut(s) 437, 521
CviAII CATG 8 cut(s) 188, 278, 332, 368, 739, 832, 855, 906
CviJI RGCY 9 cut(s) 123, 131, 148, 449, 474, 480, 535, 672, 852
CviKI_1 RGCY 9 cut(s) 123, 131, 148, 449, 474, 480, 535, 672, 852
CviQI GTAC 2 cut(s) 437, 521
DdeI CTNAG 1 cut(s) 327
DinI GGCGCC 1 cut(s) 37
DpnI GATC 1 cut(s) 702
DpnII GATC 1 cut(s) 700
DraI TTTAAA 2 cut(s) 157, 516
DrdI GACNNNNNNGTC 1 cut(s) 740
DriI GACNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 740
Eam1104I CTCTTC 1 cut(s) 580
Eam1105I GACNNNNNGTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 580
Eco130I CCWWGG 1 cut(s) 238
Eco24I GRGCYC 1 cut(s) 150
EcoRII CCWGG 1 cut(s) 480
EcoT14I CCWWGG 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 150
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 8 cut(s) 191, 281, 335, 371, 742, 835, 858, 909
FaqI GGGAC 1 cut(s) 299
FatI CATG 8 cut(s) 187, 277, 331, 367, 738, 831, 854, 905
FauI CCCGC 1 cut(s) 137
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 4 cut(s) 97, 475, 478, 533
FokI GGATG 3 cut(s) 410, 428, 663
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 4 cut(s) 97, 475, 478, 533
FspBI CTAG 4 cut(s) 246, 303, 524, 849
GlaI GCGC 2 cut(s) 37, 172
GluI GCNGC 4 cut(s) 97, 475, 478, 533
GsaI CCCAGC 1 cut(s) 768
GsuI CTGGAG 2 cut(s) 525, 882
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 4 cut(s) 131, 148, 449, 480
HapII CCGG 3 cut(s) 141, 203, 445
HhaI GCGC 2 cut(s) 38, 173
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 8 cut(s) 191, 281, 335, 371, 742, 835, 858, 909
Hin6I GCGC 2 cut(s) 36, 171
HinP1I GCGC 2 cut(s) 36, 171
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HpaII CCGG 3 cut(s) 141, 203, 445
HphI GGTGA 2 cut(s) 718, 783
Hpy166II GTNNAC 3 cut(s) 437, 439, 454
Hpy188I TCNGA 5 cut(s) 90, 328, 666, 868, 913
Hpy188III TCNNGA 6 cut(s) 368, 504, 571, 680, 777, 899
Hpy8I GTNNAC 3 cut(s) 437, 439, 454
HpyAV CCTTC 4 cut(s) 234, 467, 868, 883
HpyCH4III ACNGT 3 cut(s) 68, 194, 274
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 5 cut(s) 290, 339, 351, 374, 631
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpyF3I CTNAG 1 cut(s) 327
HpySE526I ACGT 1 cut(s) 519
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 8 cut(s) 191, 281, 335, 371, 742, 835, 858, 909
HspAI GCGC 2 cut(s) 36, 171
KasI GGCGCC 1 cut(s) 35
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 1 cut(s) 700
Lsp1109I GCAGC 2 cut(s) 108, 461
LweI GCATC 2 cut(s) 299, 361
MaeI CTAG 4 cut(s) 246, 303, 524, 849
MaeII ACGT 1 cut(s) 519
MaeIII GTNAC 2 cut(s) 136, 655
MalI GATC 1 cut(s) 702
MbiI CCGCTC 2 cut(s) 390, 532
MboI GATC 1 cut(s) 700
MboII GAAGA 4 cut(s) 315, 456, 597, 837
MfeI CAATTG 1 cut(s) 375
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 8 cut(s) 26, 152, 158, 217, 375, 648, 769, 973
Mly113I GGCGCC 1 cut(s) 36
MlyI GAGTC 3 cut(s) 24, 173, 228
MnlI CCTC 6 cut(s) 500, 575, 581, 584, 926, 952
MseI TTAA 6 cut(s) 156, 269, 384, 515, 717, 933
MslI CAYNNNNRTG 3 cut(s) 192, 359, 681
MspCI CTTAAG 1 cut(s) 383
MspI CCGG 3 cut(s) 141, 203, 445
MspR9I CCNGG 2 cut(s) 446, 482
MunI CAATTG 1 cut(s) 375
MvaI CCWGG 1 cut(s) 482
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 1 cut(s) 287
NarI GGCGCC 1 cut(s) 36
NciI CCSGG 1 cut(s) 446
NdeII GATC 1 cut(s) 700
NlaIII CATG 8 cut(s) 191, 281, 335, 371, 742, 835, 858, 909
NlaIV GGNNCC 3 cut(s) 37, 148, 207
NmuCI GTSAC 1 cut(s) 655
NspI RCATGY 1 cut(s) 335
PagI TCATGA 1 cut(s) 367
PfeI GAWTC 7 cut(s) 320, 408, 723, 780, 788, 842, 923
PkrI GCNGC 4 cut(s) 98, 476, 479, 534
PleI GAGTC 3 cut(s) 23, 172, 228
PluTI GGCGCC 1 cut(s) 39
PpsI GAGTC 3 cut(s) 23, 172, 228
Psp6I CCWGG 1 cut(s) 480
PspFI CCCAGC 1 cut(s) 764
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 3 cut(s) 37, 148, 207
PspOMI GGGCCC 1 cut(s) 146
PspPI GGNCC 4 cut(s) 129, 146, 147, 447
RsaI GTAC 2 cut(s) 438, 522
RsaNI GTAC 2 cut(s) 437, 521
RseI CAYNNNNRTG 3 cut(s) 192, 359, 681
SaqAI TTAA 6 cut(s) 156, 269, 384, 515, 717, 933
SatI GCNGC 4 cut(s) 97, 475, 478, 533
Sau3AI GATC 1 cut(s) 700
Sau96I GGNCC 4 cut(s) 129, 146, 147, 447
SchI GAGTC 3 cut(s) 24, 173, 228
ScrFI CCNGG 2 cut(s) 446, 482
SduI GDGCHC 1 cut(s) 150
SfaNI GCATC 2 cut(s) 299, 361
SfcI CTRYAG 1 cut(s) 981
SfoI GGCGCC 1 cut(s) 37
SmiI ATTTAAAT 1 cut(s) 157
SmiMI CAYNNNNRTG 3 cut(s) 192, 359, 681
SmlI CTYRAG 3 cut(s) 383, 569, 917
SmoI CTYRAG 3 cut(s) 383, 569, 917
SpeI ACTAGT 1 cut(s) 523
Sse9I AATT 8 cut(s) 26, 152, 158, 217, 375, 648, 769, 973
SsiI CCGC 5 cut(s) 144, 306, 390, 477, 532
SspDI GGCGCC 1 cut(s) 35
SspI AATATT 1 cut(s) 754
SspMI CTAG 4 cut(s) 246, 303, 524, 849
StyD4I CCNGG 2 cut(s) 444, 480
StyI CCWWGG 1 cut(s) 238
SwaI ATTTAAAT 1 cut(s) 157
TaaI ACNGT 3 cut(s) 68, 194, 274
TaiI ACGT 1 cut(s) 522
TaqI TCGA 2 cut(s) 23, 617
TasI AATT 8 cut(s) 26, 152, 158, 217, 375, 648, 769, 973
TatI WGTACW 1 cut(s) 436
TauI GCSGC 2 cut(s) 480, 535
TfiI GAWTC 7 cut(s) 320, 408, 723, 780, 788, 842, 923
Tru1I TTAA 6 cut(s) 156, 269, 384, 515, 717, 933
Tru9I TTAA 6 cut(s) 156, 269, 384, 515, 717, 933
TscAI CASTG 2 cut(s) 199, 664
TseFI GTSAC 1 cut(s) 655
TseI GCWGC 2 cut(s) 96, 474
Tsp45I GTSAC 1 cut(s) 655
TspDTI ATGAA 3 cut(s) 266, 527, 614
TspRI CASTG 2 cut(s) 199, 664
Vha464I CTTAAG 1 cut(s) 383
XapI RAATTY 2 cut(s) 152, 769
XceI RCATGY 1 cut(s) 335
XcmI CCANNNNNNNNNTGG 2 cut(s) 123, 157
XspI CTAG 4 cut(s) 246, 303, 524, 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.