Rmu_ssc0000281.1_g000003

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000281.1
N/A
Physical Location & Seq
Forward (+)
5056 .. 7963
2908 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 765 bp
atgtcagagacccatttcaacaagggtgaccaagtcgaggtcacaacctccgtccacggcggctccaccggcccctactacccagccaccgtgctccgtccagtcatcaaagacaaggcccgcatcctagtccagtaccaaaccctaacggcccacggcggaggcacaaagcccttgaaggagattgtggaggtcggcaacgtgcggcccatgcctccccgggagctccaccggccgttcaaggttggcgatgacgtggacgcgttcagggcaaaagggtggtatcgtgggacggtgagggagattttggagaattcaaagtacttggtggtgttggatggtaaagctgacctggaatttgagcgtgaccattttgatttgagggttcatagagagtgggatgatggctcttgggtccctcctcttccacaagagccggccagaccagcacaccaggccgagcactgcctaacctcacccagtcgcccgccatgcccagtcgctgcccagcctcacccaatcgccctccgcgctattgccccctcaccagcagccccgcgtctcgcgcctgccagcccgccccacgcagcaagcctgcagcctgtcctcgatctcgccctgcagcctgcccaccctcgatccatcagcagccccgcgtcccgtgcctgcgcagcccaccccatgcagcaagcctgcagcctgcccaccagcgcccaccctcgatctcacctgcagcctgcccaccctcgatctaccaacaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

254

Amino Acids

27.76

Weight (kDa)

9.12

Isoelectric Point (pI)

58.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 9 - 73 8.4e-11 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 738
Acc16I TGCGCA 1 cut(s) 670
Acc36I ACCTGC 1 cut(s) 738
AccII CGCG 5 cut(s) 263, 531, 559, 566, 656
AciI CCGC 9 cut(s) 60, 121, 159, 205, 488, 529, 557, 578, 654
AclWI GGATC 1 cut(s) 633
AcoI YGGCCR 2 cut(s) 233, 438
AcsI RAATTY 2 cut(s) 313, 356
AfaI GTAC 2 cut(s) 137, 323
AfiI CCNNNNNNNGG 1 cut(s) 37
AflIII ACRYGT 1 cut(s) 261
AgsI TTSAA 4 cut(s) 19, 178, 241, 318
AjiI CACGTC 1 cut(s) 256
AjnI CCWGG 2 cut(s) 351, 453
AluBI AGCT 2 cut(s) 226, 347
AluI AGCT 2 cut(s) 226, 347
Alw21I GWGCWC 3 cut(s) 96, 228, 465
Alw26I GTCTC 2 cut(s) 2, 566
AlwI GGATC 1 cut(s) 633
AlwNI CAGNNNCTG 1 cut(s) 503
Ama87I CYCGRG 1 cut(s) 219
AoxI GGCC 7 cut(s) 70, 117, 150, 206, 233, 438, 456
ApoI RAATTY 2 cut(s) 313, 356
ArsI GACNNNNNNTTYG 2 cut(s) 341, 373
AspLEI GCGC 4 cut(s) 533, 568, 671, 713
AspS9I GGNCC 5 cut(s) 71, 118, 151, 207, 415
AsuC2I CCSGG 2 cut(s) 220, 221
AsuHPI GGTGA 6 cut(s) 38, 307, 468, 506, 537, 719
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 1 cut(s) 415
BanII GRGCYC 1 cut(s) 228
Bbv12I GWGCWC 3 cut(s) 96, 228, 465
BccI CCATC 3 cut(s) 332, 398, 650
BceAI ACGGC 4 cut(s) 73, 165, 172, 220
BciT130I CCWGG 2 cut(s) 353, 455
BcnI CCSGG 2 cut(s) 220, 221
BcoDI GTCTC 2 cut(s) 2, 566
BfaI CTAG 1 cut(s) 128
BfmI CTRYAG 4 cut(s) 596, 620, 694, 731
BfoI RGCGCY 1 cut(s) 714
BfuAI ACCTGC 1 cut(s) 738
BmcAI AGTACT 1 cut(s) 323
Bme1390I CCNGG 4 cut(s) 220, 221, 353, 455
Bme18I GGWCC 1 cut(s) 415
BmeT110I CYCGRG 1 cut(s) 219
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 5 cut(s) 71, 118, 151, 207, 415
BmiI GGNNCC 4 cut(s) 64, 73, 416, 417
BmrFI CCNGG 4 cut(s) 220, 221, 353, 455
BmrI ACTGGG 2 cut(s) 474, 491
BmsI GCATC 1 cut(s) 132
BmuI ACTGGG 2 cut(s) 474, 491
BpuMI CCSGG 2 cut(s) 220, 221
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 4 cut(s) 55, 154, 218, 219
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse118I RCCGGY 3 cut(s) 68, 231, 436
Bse1I ACTGG 4 cut(s) 101, 133, 480, 497
BseBI CCWGG 2 cut(s) 353, 455
BseDI CCNNGG 4 cut(s) 55, 154, 218, 219
BseGI GGATG 3 cut(s) 123, 343, 406
BseLI CCNNNNNNNGG 1 cut(s) 37
BseNI ACTGG 4 cut(s) 101, 133, 480, 497
BseRI GAGGAG 1 cut(s) 411
BseX3I CGGCCG 1 cut(s) 233
BseYI CCCAGC 2 cut(s) 82, 507
Bsh1236I CGCG 5 cut(s) 263, 531, 559, 566, 656
Bsh1285I CGRYCG 1 cut(s) 236
BshFI GGCC 7 cut(s) 72, 119, 152, 208, 235, 440, 458
BsiEI CGRYCG 1 cut(s) 236
BsiHKAI GWGCWC 3 cut(s) 96, 228, 465
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 4 cut(s) 69, 220, 232, 437
BslFI GGGAC 3 cut(s) 304, 401, 643
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 2 cut(s) 2, 566
BsmBI CGTCTC 1 cut(s) 566
BsmFI GGGAC 3 cut(s) 304, 401, 643
BsnI GGCC 7 cut(s) 72, 119, 152, 208, 235, 440, 458
Bso31I GGTCTC 1 cut(s) 2
BsoBI CYCGRG 1 cut(s) 219
Bsp1286I GDGCHC 3 cut(s) 96, 228, 465
Bsp143I GATC 4 cut(s) 610, 638, 722, 749
BspACI CCGC 9 cut(s) 60, 121, 159, 205, 488, 529, 557, 578, 654
BspANI GGCC 7 cut(s) 72, 119, 152, 208, 235, 440, 458
BspFNI CGCG 5 cut(s) 263, 531, 559, 566, 656
BspLI GGNNCC 4 cut(s) 64, 73, 416, 417
BspMAI CTGCAG 4 cut(s) 600, 624, 698, 735
BspMI ACCTGC 1 cut(s) 738
BspPI GGATC 1 cut(s) 633
BspTNI GGTCTC 1 cut(s) 2
BsrFI RCCGGY 3 cut(s) 68, 231, 436
BsrI ACTGG 4 cut(s) 101, 133, 480, 497
BssAI RCCGGY 3 cut(s) 68, 231, 436
BssECI CCNNGG 4 cut(s) 55, 154, 218, 219
BssMI GATC 4 cut(s) 610, 638, 722, 749
Bst2UI CCWGG 2 cut(s) 353, 455
Bst4CI ACNGT 2 cut(s) 91, 295
Bst6I CTCTTC 1 cut(s) 429
BstDSI CCRYGG 2 cut(s) 55, 154
BstEII GGTNACC 1 cut(s) 26
BstF5I GGATG 3 cut(s) 123, 343, 406
BstFNI CGCG 5 cut(s) 263, 531, 559, 566, 656
BstH2I RGCGCY 1 cut(s) 714
BstHHI GCGC 4 cut(s) 533, 568, 671, 713
BstKTI GATC 4 cut(s) 613, 641, 725, 752
BstMAI GTCTC 2 cut(s) 2, 566
BstMBI GATC 4 cut(s) 610, 638, 722, 749
BstMCI CGRYCG 1 cut(s) 236
BstNI CCWGG 2 cut(s) 353, 455
BstPI GGTNACC 1 cut(s) 26
BstSCI CCNGG 4 cut(s) 218, 219, 351, 453
BstSFI CTRYAG 4 cut(s) 596, 620, 694, 731
BstUI CGCG 5 cut(s) 263, 531, 559, 566, 656
BstZI CGGCCG 1 cut(s) 233
BsuRI GGCC 7 cut(s) 72, 119, 152, 208, 235, 440, 458
BtgI CCRYGG 2 cut(s) 55, 154
BtgZI GCGATG 1 cut(s) 264
BtrI CACGTC 1 cut(s) 256
BtsCI GGATG 3 cut(s) 123, 343, 406
BtsI GCAGTG 1 cut(s) 463
BtsIMutI CAGTG 1 cut(s) 463
BveI ACCTGC 1 cut(s) 738
CaiI CAGNNNCTG 1 cut(s) 503
CfoI GCGC 4 cut(s) 533, 568, 671, 713
Cfr10I RCCGGY 3 cut(s) 68, 231, 436
Cfr13I GGNCC 5 cut(s) 71, 118, 151, 207, 415
Cfr9I CCCGGG 1 cut(s) 219
CseI GACGC 3 cut(s) 269, 548, 645
Csp6I GTAC 2 cut(s) 136, 322
CviAII CATG 3 cut(s) 211, 492, 682
CviQI GTAC 2 cut(s) 136, 322
DpnI GATC 4 cut(s) 612, 640, 724, 751
DpnII GATC 4 cut(s) 610, 638, 722, 749
EaeI YGGCCR 2 cut(s) 233, 438
EagI CGGCCG 1 cut(s) 233
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
EciI GGCGGA 1 cut(s) 174
Ecl136II GAGCTC 1 cut(s) 226
EclXI CGGCCG 1 cut(s) 233
Eco24I GRGCYC 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 2
Eco47I GGWCC 1 cut(s) 415
Eco52I CGGCCG 1 cut(s) 233
Eco53kI GAGCTC 1 cut(s) 226
Eco88I CYCGRG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 415
EcoO65I GGTNACC 1 cut(s) 26
EcoRI GAATTC 1 cut(s) 313
EcoRII CCWGG 2 cut(s) 351, 453
EcoT38I GRGCYC 1 cut(s) 228
Esp3I CGTCTC 1 cut(s) 566
FaeI CATG 3 cut(s) 214, 495, 685
FaiI YATR 4 cut(s) 212, 390, 493, 683
FaqI GGGAC 3 cut(s) 304, 401, 643
FatI CATG 3 cut(s) 210, 491, 681
FauI CCCGC 5 cut(s) 128, 495, 564, 585, 661
FokI GGATG 3 cut(s) 110, 350, 413
FriOI GRGCYC 1 cut(s) 228
FspBI CTAG 1 cut(s) 128
FspI TGCGCA 1 cut(s) 670
GlaI GCGC 4 cut(s) 532, 567, 670, 712
GsaI CCCAGC 2 cut(s) 86, 511
HaeII RGCGCY 1 cut(s) 714
HaeIII GGCC 7 cut(s) 72, 119, 152, 208, 235, 440, 458
HapII CCGG 4 cut(s) 69, 220, 232, 437
HgaI GACGC 3 cut(s) 269, 548, 645
HhaI GCGC 4 cut(s) 533, 568, 671, 713
Hin1II CATG 3 cut(s) 214, 495, 685
Hin6I GCGC 4 cut(s) 531, 566, 669, 711
HinP1I GCGC 4 cut(s) 531, 566, 669, 711
HpaII CCGG 4 cut(s) 69, 220, 232, 437
HphI GGTGA 6 cut(s) 38, 307, 468, 506, 537, 719
Hpy166II GTNNAC 2 cut(s) 55, 259
Hpy188I TCNGA 1 cut(s) 7
Hpy8I GTNNAC 2 cut(s) 55, 259
HpyAV CCTTC 1 cut(s) 172
HpyCH4III ACNGT 2 cut(s) 91, 295
HpyCH4IV ACGT 2 cut(s) 201, 255
HpyCH4V TGCA 5 cut(s) 598, 622, 685, 696, 733
HpySE526I ACGT 2 cut(s) 201, 255
Hsp92II CATG 3 cut(s) 214, 495, 685
HspAI GCGC 4 cut(s) 531, 566, 669, 711
KflI GGGWCCC 1 cut(s) 415
KroI GCCGGC 1 cut(s) 436
KroNI GCCGGC 1 cut(s) 438
Kzo9I GATC 4 cut(s) 610, 638, 722, 749
LmnI GCTCC 4 cut(s) 68, 99, 223, 231
LweI GCATC 1 cut(s) 132
MaeI CTAG 1 cut(s) 128
MaeII ACGT 2 cut(s) 201, 255
MaeIII GTNAC 3 cut(s) 26, 40, 365
MalI GATC 4 cut(s) 612, 640, 724, 751
MboI GATC 4 cut(s) 610, 638, 722, 749
MboII GAAGA 1 cut(s) 416
MhlI GDGCHC 3 cut(s) 96, 228, 465
MluCI AATT 2 cut(s) 313, 356
MluI ACGCGT 1 cut(s) 261
MmeI TCCRAC 1 cut(s) 315
MroNI GCCGGC 1 cut(s) 436
MspI CCGG 4 cut(s) 69, 220, 232, 437
MspR9I CCNGG 4 cut(s) 220, 221, 353, 455
MvaI CCWGG 2 cut(s) 353, 455
MvnI CGCG 5 cut(s) 263, 531, 559, 566, 656
NaeI GCCGGC 1 cut(s) 438
NciI CCSGG 2 cut(s) 220, 221
NdeII GATC 4 cut(s) 610, 638, 722, 749
NgoMIV GCCGGC 1 cut(s) 436
NlaIII CATG 3 cut(s) 214, 495, 685
NlaIV GGNNCC 4 cut(s) 64, 73, 416, 417
NmeAIII GCCGAG 1 cut(s) 484
NmuCI GTSAC 3 cut(s) 26, 40, 365
NsbI TGCGCA 1 cut(s) 670
PaqCI CACCTGC 1 cut(s) 738
PdiI GCCGGC 1 cut(s) 438
PflFI GACNNNGTC 1 cut(s) 32
PpuMI RGGWCCY 1 cut(s) 415
Psp124BI GAGCTC 1 cut(s) 228
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 2 cut(s) 351, 453
PspEI GGTNACC 1 cut(s) 26
PspFI CCCAGC 2 cut(s) 82, 507
PspGI CCWGG 2 cut(s) 351, 453
PspN4I GGNNCC 4 cut(s) 64, 73, 416, 417
PspPI GGNCC 5 cut(s) 71, 118, 151, 207, 415
PspPPI RGGWCCY 1 cut(s) 415
PstI CTGCAG 4 cut(s) 600, 624, 698, 735
PstNI CAGNNNCTG 1 cut(s) 503
PsyI GACNNNGTC 1 cut(s) 32
RsaI GTAC 2 cut(s) 137, 323
RsaNI GTAC 2 cut(s) 136, 322
SacI GAGCTC 1 cut(s) 228
Sau3AI GATC 4 cut(s) 610, 638, 722, 749
Sau96I GGNCC 5 cut(s) 71, 118, 151, 207, 415
ScaI AGTACT 1 cut(s) 323
ScrFI CCNGG 4 cut(s) 220, 221, 353, 455
SduI GDGCHC 3 cut(s) 96, 228, 465
SfaNI GCATC 1 cut(s) 132
SfcI CTRYAG 4 cut(s) 596, 620, 694, 731
SinI GGWCC 1 cut(s) 415
SmaI CCCGGG 1 cut(s) 221
Sse9I AATT 2 cut(s) 313, 356
SsiI CCGC 9 cut(s) 60, 121, 159, 205, 488, 529, 557, 578, 654
SspMI CTAG 1 cut(s) 128
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 4 cut(s) 218, 219, 351, 453
TaaI ACNGT 2 cut(s) 91, 295
TaiI ACGT 2 cut(s) 204, 258
TaqI TCGA 5 cut(s) 36, 609, 637, 721, 748
TasI AATT 2 cut(s) 313, 356
TatI WGTACW 1 cut(s) 321
TauI GCSGC 2 cut(s) 63, 208
TscAI CASTG 1 cut(s) 470
TseFI GTSAC 3 cut(s) 26, 40, 365
Tsp45I GTSAC 3 cut(s) 26, 40, 365
TspDTI ATGAA 1 cut(s) 377
TspGWI ACGGA 2 cut(s) 40, 86
TspMI CCCGGG 1 cut(s) 219
TspRI CASTG 1 cut(s) 470
Tth111I GACNNNGTC 1 cut(s) 32
VpaK11BI GGWCC 1 cut(s) 415
XapI RAATTY 2 cut(s) 313, 356
XmaI CCCGGG 1 cut(s) 219
XspI CTAG 1 cut(s) 128
ZrmI AGTACT 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.