Rmu_ssc0000289.1_g000027

Transmembrane Fragile-X-F protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000289.1
N/A
Physical Location & Seq
Reverse (-)
141068 .. 143591
2524 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1254 bp
atgagtcgttccccttcattttcagtgaagtcggaatttagtccaaagcttgatccagaatctttgcagcaatgggttgtggctttttgtatcatcagatttgatcttgaacagggtcagcttattgaagagtgttacccacctggttgtcttacgcaagatgaggagcttgaaatcgcttttagttcattcccagattccgtttcccagcatcagaatcgctcaagcattcatgactgtatattctttttccggtttcgaaggcgggaacgtcttcaaatggacaatgtggcttccacagagatcactgagagtaataagaagttacctacaaattccccagatgtaaaaccccctagaagttcaaaaagtaacaacatttctgatacttcgagatacatgtatggttatgtctttaatcgacagagacatgatgagaggctaaaacgaggtggggagcagaagtctgtggtaattttccaaataagagtagctacttcatcttctacaactattagatatcttgactggaacagcggtctagtagtttctgcagaagaggatcagcatcaagatagaatgtgtggtctgcaagacattggcgggcatgttatgaaaattccggtgattggttttcaagttctcctttgtatgcgccttgagggaacccctgatcgtgctagacatatcccacttccggttctattctctcctctttttctactacaaggtgttggtgtaatatttgctgcatctaggttggtggagaaaattgtacttctactgcgtagtggagctggcacaggattatattttagattttcttccagagcacacgattgcttggggtttttgcaccatggttccaggctacttggctggtggtcaatagatgaaggaagttgtgaggaacatgcccgactgtttcatgagggggcttcaggatacaacactttctgtggttatccacctgagatagtgaagaaaatgcctaaaaaggatctcactgaagaggtatggagacttcaagcagctcttggtgaacagacagaaatcacaaaatacagtcagcaggagtatgaaagacttcaaaatgaaaaagtgttatgccgtgtttgctttgagggagatatcagtgtggtcctgctaccatgtaggcatcgtgttctttgcagtacctgctgtgacaagtgtaaaaaatgcccaatatgccgtgtttctattgaggagcgcttacctgtgtatgatgtctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_185A zf-C3HC4_3

Protein Analysis

417

Amino Acids

47.71

Weight (kDa)

7.14

Isoelectric Point (pI)

63.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_185A PF10269 161 - 242 1.2e-14 Transmembrane Fragile-X-F protein
zf-C3HC4_3 PF13920 367 - 410 2.7e-11 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1187
AciI CCGC 3 cut(s) 265, 537, 603
AclWI GGATC 3 cut(s) 47, 570, 1008
AcsI RAATTY 3 cut(s) 35, 334, 618
AcuI CTGAAG 2 cut(s) 924, 1029
AfaI GTAC 2 cut(s) 777, 1177
AfeI AGCGCT 1 cut(s) 1232
AfiI CCNNNNNNNGG 2 cut(s) 629, 697
AflIII ACRYGT 1 cut(s) 399
AgsI TTSAA 8 cut(s) 110, 128, 173, 278, 366, 638, 1028, 1091
AjnI CCWGG 2 cut(s) 142, 866
AluBI AGCT 6 cut(s) 49, 121, 169, 494, 797, 1034
AluI AGCT 6 cut(s) 49, 121, 169, 494, 797, 1034
Alw21I GWGCWC 1 cut(s) 835
Alw26I GTCTC 2 cut(s) 421, 1015
AlwI GGATC 3 cut(s) 47, 570, 1008
AlwNI CAGNNNCTG 1 cut(s) 1179
Aor51HI AGCGCT 1 cut(s) 1232
ApeKI GCWGC 3 cut(s) 67, 749, 1031
ApoI RAATTY 3 cut(s) 35, 334, 618
Asp700I GAANNNNTTC 2 cut(s) 273, 1086
AspLEI GCGC 2 cut(s) 657, 1233
AspS9I GGNCC 1 cut(s) 1141
AsuHPI GGTGA 2 cut(s) 637, 1052
AsuII TTCGAA 1 cut(s) 259
AvaII GGWCC 1 cut(s) 1141
BarI GAAGNNNNNNTAC 2 cut(s) 1008, 1040
BbsI GAAGAC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 835
BbvI GCAGC 3 cut(s) 79, 736, 1043
BceAI ACGGC 2 cut(s) 1095, 1197
BcgI CGANNNNNNTGC 2 cut(s) 200, 234
BciT130I CCWGG 2 cut(s) 144, 868
BciVI GTATCC 1 cut(s) 938
BcoDI GTCTC 2 cut(s) 421, 1015
BfaI CTAG 5 cut(s) 357, 542, 681, 756, 1252
BfmI CTRYAG 1 cut(s) 552
BfoI RGCGCY 1 cut(s) 1234
BfuAI ACCTGC 1 cut(s) 1187
BfuI GTATCC 1 cut(s) 938
BisI GCNGC 3 cut(s) 68, 750, 1032
BlsI GCNGC 3 cut(s) 69, 751, 1033
Bme1390I CCNGG 2 cut(s) 144, 868
Bme18I GGWCC 1 cut(s) 1141
BmgT120I GGNCC 1 cut(s) 1141
BmiI GGNNCC 2 cut(s) 667, 865
BmrFI CCNGG 2 cut(s) 144, 868
BmsI GCATC 4 cut(s) 220, 577, 761, 1168
BpiI GAAGAC 1 cut(s) 266
Bpu14I TTCGAA 1 cut(s) 259
BpuEI CTTGAG 2 cut(s) 208, 680
BsaBI GATNNNNATC 1 cut(s) 567
BsaJI CCNNGG 1 cut(s) 859
BsaWI WCCGGW 3 cut(s) 252, 622, 697
BsaXI ACNNNNNCTCC 2 cut(s) 449, 479
Bsc4I CCNNNNNNNGG 2 cut(s) 629, 697
Bse1I ACTGG 1 cut(s) 533
Bse3DI GCAATG 1 cut(s) 77
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 2 cut(s) 144, 868
BseDI CCNNGG 1 cut(s) 859
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 2 cut(s) 629, 697
BseMI GCAATG 1 cut(s) 77
BseMII CTCAG 2 cut(s) 300, 963
BseNI ACTGG 1 cut(s) 533
BseRI GAGGAG 3 cut(s) 179, 702, 1241
BseXI GCAGC 3 cut(s) 79, 736, 1043
BseYI CCCAGC 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 835
BsiSI CCGG 3 cut(s) 253, 623, 698
BslI CCNNNNNNNGG 2 cut(s) 629, 697
BsmAI GTCTC 2 cut(s) 421, 1015
BsmI GAATGC 1 cut(s) 228
Bsp119I TTCGAA 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 835
Bsp143I GATC 6 cut(s) 52, 103, 303, 562, 673, 1000
Bsp19I CCATGG 1 cut(s) 859
BspACI CCGC 3 cut(s) 265, 537, 603
BspCNI CTCAG 2 cut(s) 301, 964
BspHI TCATGA 2 cut(s) 232, 928
BspLI GGNNCC 2 cut(s) 667, 865
BspMAI CTGCAG 1 cut(s) 556
BspMI ACCTGC 1 cut(s) 1187
BspPI GGATC 3 cut(s) 47, 570, 1008
BspT104I TTCGAA 1 cut(s) 259
BsrDI GCAATG 1 cut(s) 77
BsrI ACTGG 1 cut(s) 533
BssECI CCNNGG 1 cut(s) 859
BssMI GATC 6 cut(s) 52, 103, 303, 562, 673, 1000
BssT1I CCWWGG 1 cut(s) 859
Bst2UI CCWGG 2 cut(s) 144, 868
Bst4CI ACNGT 3 cut(s) 239, 924, 1067
Bst6I CTCTTC 3 cut(s) 123, 552, 1005
BstAPI GCANNNNNTGC 1 cut(s) 1179
BstBI TTCGAA 1 cut(s) 259
BstC8I GCNNGC 2 cut(s) 605, 799
BstDEI CTNAG 2 cut(s) 309, 972
BstDSI CCRYGG 1 cut(s) 859
BstH2I RGCGCY 1 cut(s) 1234
BstHHI GCGC 2 cut(s) 657, 1233
BstKTI GATC 6 cut(s) 55, 106, 306, 565, 676, 1003
BstMAI GTCTC 2 cut(s) 421, 1015
BstMBI GATC 6 cut(s) 52, 103, 303, 562, 673, 1000
BstMWI GCNNNNNNNGC 3 cut(s) 1116, 1179, 1209
BstNI CCWGG 2 cut(s) 144, 868
BstNSI RCATGY 3 cut(s) 403, 611, 917
BstSCI CCNGG 2 cut(s) 142, 866
BstSFI CTRYAG 1 cut(s) 552
BstV1I GCAGC 3 cut(s) 79, 736, 1043
BstV2I GAAGAC 1 cut(s) 266
BstX2I RGATCY 1 cut(s) 1000
BstYI RGATCY 1 cut(s) 1000
BsuI GTATCC 1 cut(s) 938
BtgI CCRYGG 1 cut(s) 859
BtsIMutI CAGTG 4 cut(s) 30, 306, 1005, 1141
BveI ACCTGC 1 cut(s) 1187
Cac8I GCNNGC 2 cut(s) 605, 799
CaiI CAGNNNCTG 1 cut(s) 1179
CciI TCATGA 2 cut(s) 232, 928
CfoI GCGC 2 cut(s) 657, 1233
Cfr13I GGNCC 1 cut(s) 1141
CsiI ACCWGGT 1 cut(s) 142
Csp6I GTAC 2 cut(s) 776, 1176
CviAII CATG 8 cut(s) 233, 400, 431, 608, 860, 914, 929, 1152
CviQI GTAC 2 cut(s) 776, 1176
DdeI CTNAG 2 cut(s) 309, 972
DpnI GATC 6 cut(s) 54, 105, 305, 564, 675, 1002
DpnII GATC 6 cut(s) 52, 103, 303, 562, 673, 1000
Eam1104I CTCTTC 3 cut(s) 123, 552, 1005
EarI CTCTTC 3 cut(s) 123, 552, 1005
Eco130I CCWWGG 1 cut(s) 859
Eco32I GATATC 2 cut(s) 521, 1132
Eco47I GGWCC 1 cut(s) 1141
Eco47III AGCGCT 1 cut(s) 1232
Eco57I CTGAAG 2 cut(s) 924, 1029
EcoRII CCWGG 2 cut(s) 142, 866
EcoRV GATATC 2 cut(s) 521, 1132
EcoT14I CCWWGG 1 cut(s) 859
ErhI CCWWGG 1 cut(s) 859
FaeI CATG 8 cut(s) 236, 403, 434, 611, 863, 917, 932, 1155
FalI AAGNNNNNCTT 4 cut(s) 630, 662, 1020, 1052
FatI CATG 8 cut(s) 232, 399, 430, 607, 859, 913, 928, 1151
FauI CCCGC 2 cut(s) 258, 596
Fnu4HI GCNGC 3 cut(s) 68, 750, 1032
Fsp4HI GCNGC 3 cut(s) 68, 750, 1032
FspBI CTAG 5 cut(s) 357, 542, 681, 756, 1252
GlaI GCGC 2 cut(s) 656, 1232
GluI GCNGC 3 cut(s) 68, 750, 1032
GsaI CCCAGC 1 cut(s) 211
HaeII RGCGCY 1 cut(s) 1234
HapII CCGG 3 cut(s) 253, 623, 698
HhaI GCGC 2 cut(s) 657, 1233
Hin1II CATG 8 cut(s) 236, 403, 434, 611, 863, 917, 932, 1155
Hin6I GCGC 2 cut(s) 655, 1231
HinP1I GCGC 2 cut(s) 655, 1231
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 4 cut(s) 4, 59, 197, 217
HpaII CCGG 3 cut(s) 253, 623, 698
HphI GGTGA 2 cut(s) 637, 1052
Hpy166II GTNNAC 1 cut(s) 1043
Hpy188I TCNGA 4 cut(s) 34, 98, 216, 385
Hpy188III TCNNGA 9 cut(s) 56, 107, 233, 393, 524, 572, 828, 929, 942
Hpy8I GTNNAC 1 cut(s) 1043
HpyAV CCTTC 3 cut(s) 24, 255, 890
HpyCH4III ACNGT 3 cut(s) 239, 924, 1067
HpyCH4IV ACGT 1 cut(s) 271
HpyCH4V TGCA 6 cut(s) 67, 554, 592, 752, 856, 1173
HpyF10VI GCNNNNNNNGC 3 cut(s) 1116, 1179, 1209
HpyF3I CTNAG 2 cut(s) 309, 972
HpySE526I ACGT 1 cut(s) 271
Hsp92II CATG 8 cut(s) 236, 403, 434, 611, 863, 917, 932, 1155
HspAI GCGC 2 cut(s) 655, 1231
Kzo9I GATC 6 cut(s) 52, 103, 303, 562, 673, 1000
LmnI GCTCC 4 cut(s) 166, 457, 794, 1228
Lsp1109I GCAGC 3 cut(s) 79, 736, 1043
LweI GCATC 4 cut(s) 220, 577, 761, 1168
MabI ACCWGGT 1 cut(s) 142
MaeI CTAG 5 cut(s) 357, 542, 681, 756, 1252
MaeII ACGT 1 cut(s) 271
MaeIII GTNAC 4 cut(s) 134, 324, 371, 1184
MalI GATC 6 cut(s) 54, 105, 305, 564, 675, 1002
MboI GATC 6 cut(s) 52, 103, 303, 562, 673, 1000
MboII GAAGA 7 cut(s) 140, 266, 495, 569, 816, 994, 1022
MflI RGATCY 1 cut(s) 1000
MhlI GDGCHC 1 cut(s) 835
MluCI AATT 5 cut(s) 35, 334, 474, 618, 771
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 12
MroXI GAANNNNTTC 2 cut(s) 273, 1086
MseI TTAA 1 cut(s) 417
MspA1I CMGCKG 1 cut(s) 537
MspI CCGG 3 cut(s) 253, 623, 698
MspR9I CCNGG 2 cut(s) 144, 868
Mva1269I GAATGC 1 cut(s) 228
MvaI CCWGG 2 cut(s) 144, 868
MwoI GCNNNNNNNGC 3 cut(s) 1116, 1179, 1209
NcoI CCATGG 1 cut(s) 859
NdeII GATC 6 cut(s) 52, 103, 303, 562, 673, 1000
NlaIII CATG 8 cut(s) 236, 403, 434, 611, 863, 917, 932, 1155
NlaIV GGNNCC 2 cut(s) 667, 865
NmuCI GTSAC 1 cut(s) 1184
NspI RCATGY 3 cut(s) 403, 611, 917
NspV TTCGAA 1 cut(s) 259
PagI TCATGA 2 cut(s) 232, 928
PciI ACATGT 1 cut(s) 399
PctI GAATGC 1 cut(s) 228
PdmI GAANNNNTTC 2 cut(s) 273, 1086
PfeI GAWTC 3 cut(s) 59, 197, 217
PkrI GCNGC 3 cut(s) 69, 751, 1033
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PscI ACATGT 1 cut(s) 399
Psp6I CCWGG 2 cut(s) 142, 866
PspFI CCCAGC 1 cut(s) 207
PspGI CCWGG 2 cut(s) 142, 866
PspN4I GGNNCC 2 cut(s) 667, 865
PspPI GGNCC 1 cut(s) 1141
PstI CTGCAG 1 cut(s) 556
PstNI CAGNNNCTG 1 cut(s) 1179
PsuI RGATCY 1 cut(s) 1000
RsaI GTAC 2 cut(s) 777, 1177
RsaNI GTAC 2 cut(s) 776, 1176
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 3 cut(s) 68, 750, 1032
Sau3AI GATC 6 cut(s) 52, 103, 303, 562, 673, 1000
Sau96I GGNCC 1 cut(s) 1141
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 2 cut(s) 144, 868
SduI GDGCHC 1 cut(s) 835
SexAI ACCWGGT 1 cut(s) 142
SfaNI GCATC 4 cut(s) 220, 577, 761, 1168
SfcI CTRYAG 1 cut(s) 552
SfuI TTCGAA 1 cut(s) 259
SinI GGWCC 1 cut(s) 1141
SmlI CTYRAG 2 cut(s) 223, 659
SmoI CTYRAG 2 cut(s) 223, 659
Sse9I AATT 5 cut(s) 35, 334, 474, 618, 771
SsiI CCGC 3 cut(s) 265, 537, 603
SspI AATATT 1 cut(s) 744
SspMI CTAG 5 cut(s) 357, 542, 681, 756, 1252
StyD4I CCNGG 2 cut(s) 142, 866
StyI CCWWGG 1 cut(s) 859
TaaI ACNGT 3 cut(s) 239, 924, 1067
TaiI ACGT 1 cut(s) 274
TaqI TCGA 3 cut(s) 259, 392, 421
TasI AATT 5 cut(s) 35, 334, 474, 618, 771
TatI WGTACW 1 cut(s) 775
TfiI GAWTC 3 cut(s) 59, 197, 217
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 4 cut(s) 30, 313, 1012, 1141
TseFI GTSAC 1 cut(s) 1184
TseI GCWGC 3 cut(s) 67, 749, 1031
Tsp45I GTSAC 1 cut(s) 1184
TspDTI ATGAA 9 cut(s) 6, 177, 221, 489, 629, 909, 917, 1095, 1110
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 4 cut(s) 30, 313, 1012, 1141
VpaK11BI GGWCC 1 cut(s) 1141
XapI RAATTY 3 cut(s) 35, 334, 618
XceI RCATGY 3 cut(s) 403, 611, 917
XmnI GAANNNNTTC 2 cut(s) 273, 1086
XspI CTAG 5 cut(s) 357, 542, 681, 756, 1252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.