Rmu_ssc0000340.1_g000004

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000340.1
OG0031019
Physical Location & Seq
Reverse (-)
35991 .. 37536
1546 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
atgatgttaaggctctcccacggccatggcatgaagcctagagaagttgctgccatgagggactgcgtcgaactgttgagtgactcggtggacgagcttcagaggtctatttcgaagatggttaactttaggagttctgggtttgagcttatgataagtgatgttcaaacttgggttagtgctgccttgacggatgagggcacctgctcggatgggtttgctggaaatggattgaatgggaatttgaagactgttgtgaggggtcggattgtgaacattgcacaattgactagcaatgccttggctttgattaacagatacgcctcgcaaatactctaccggttcaccacctttcggacctcctatttccaccaaatttttccagcaaaagcatttcaactccaggattcaggaagcgaaggaaaatcactgaaaaccggcctagaagttgatgaaccagggccaaaaccttctgcagaaaaaccggcagaagaagaagaaggaaaaaagaagaagggagaaggtagcccaaggcccagcggctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

181

Amino Acids

19.79

Weight (kDa)

7.77

Isoelectric Point (pI)

31.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 11 - 103 1.2e-24 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031019)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004947.1_g000014 Rmu_ssc0000340.1_g000004

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 212
Acc36I ACCTGC 1 cut(s) 212
AccB1I GGYRCC 1 cut(s) 200
AciI CCGC 1 cut(s) 540
AcoI YGGCCR 1 cut(s) 22
AcsI RAATTY 2 cut(s) 241, 375
AcuI CTGAAG 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 354
AgeI ACCGGT 1 cut(s) 339
AgsI TTSAA 4 cut(s) 167, 235, 247, 398
AjnI CCWGG 2 cut(s) 402, 457
AluBI AGCT 2 cut(s) 97, 148
AluI AGCT 2 cut(s) 97, 148
AlwNI CAGNNNCTG 1 cut(s) 543
AoxI GGCC 4 cut(s) 22, 439, 461, 533
ApeKI GCWGC 2 cut(s) 50, 182
ApoI RAATTY 2 cut(s) 241, 375
AsiGI ACCGGT 1 cut(s) 339
AspS9I GGNCC 3 cut(s) 357, 461, 534
AsuHPI GGTGA 1 cut(s) 337
AsuII TTCGAA 1 cut(s) 113
AvaII GGWCC 1 cut(s) 357
BaeGI GKGCMC 1 cut(s) 203
BanI GGYRCC 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 254
BbvI GCAGC 2 cut(s) 37, 169
BccI CCATC 2 cut(s) 112, 206
BceAI ACGGC 1 cut(s) 37
BciT130I CCWGG 2 cut(s) 404, 459
BfaI CTAG 3 cut(s) 39, 291, 443
BfmI CTRYAG 1 cut(s) 474
BfuAI ACCTGC 1 cut(s) 212
BisI GCNGC 3 cut(s) 51, 183, 541
BlsI GCNGC 3 cut(s) 52, 184, 542
Bme1390I CCNGG 2 cut(s) 404, 459
Bme18I GGWCC 1 cut(s) 357
BmgT120I GGNCC 3 cut(s) 357, 461, 534
BmiI GGNNCC 1 cut(s) 202
BmrFI CCNGG 2 cut(s) 404, 459
BpiI GAAGAC 1 cut(s) 254
BpmI CTGGAG 1 cut(s) 386
Bpu14I TTCGAA 1 cut(s) 113
BsaJI CCNNGG 5 cut(s) 19, 25, 300, 458, 530
BsaWI WCCGGW 1 cut(s) 339
Bsc4I CCNNNNNNNGG 1 cut(s) 354
Bse118I RCCGGY 3 cut(s) 339, 437, 484
Bse3DI GCAATG 2 cut(s) 276, 301
BseBI CCWGG 2 cut(s) 404, 459
BseDI CCNNGG 5 cut(s) 19, 25, 300, 458, 530
BseGI GGATG 2 cut(s) 199, 217
BseLI CCNNNNNNNGG 1 cut(s) 354
BseMI GCAATG 2 cut(s) 276, 301
BseSI GKGCMC 1 cut(s) 203
BseXI GCAGC 2 cut(s) 37, 169
BseYI CCCAGC 1 cut(s) 536
BshFI GGCC 4 cut(s) 24, 441, 463, 535
BshNI GGYRCC 1 cut(s) 200
BshTI ACCGGT 1 cut(s) 339
BsiSI CCGG 3 cut(s) 340, 438, 485
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 1 cut(s) 354
BsmFI GGGAC 1 cut(s) 74
BsnI GGCC 4 cut(s) 24, 441, 463, 535
Bsp119I TTCGAA 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 203
Bsp19I CCATGG 1 cut(s) 25
BspACI CCGC 1 cut(s) 540
BspANI GGCC 4 cut(s) 24, 441, 463, 535
BspLI GGNNCC 1 cut(s) 202
BspMAI CTGCAG 1 cut(s) 478
BspMI ACCTGC 1 cut(s) 212
BspT104I TTCGAA 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 200
BsrDI GCAATG 2 cut(s) 276, 301
BsrFI RCCGGY 3 cut(s) 339, 437, 484
BssAI RCCGGY 3 cut(s) 339, 437, 484
BssECI CCNNGG 5 cut(s) 19, 25, 300, 458, 530
BssT1I CCWWGG 3 cut(s) 25, 300, 530
Bst2UI CCWGG 2 cut(s) 404, 459
Bst4CI ACNGT 2 cut(s) 75, 253
BstBI TTCGAA 1 cut(s) 113
BstDSI CCRYGG 2 cut(s) 19, 25
BstF5I GGATG 2 cut(s) 199, 217
BstNI CCWGG 2 cut(s) 404, 459
BstSCI CCNGG 2 cut(s) 402, 457
BstSFI CTRYAG 1 cut(s) 474
BstSLI GKGCMC 1 cut(s) 203
BstV1I GCAGC 2 cut(s) 37, 169
BstV2I GAAGAC 1 cut(s) 254
BstXI CCANNNNNNTGG 1 cut(s) 26
BsuRI GGCC 4 cut(s) 24, 441, 463, 535
BtgI CCRYGG 2 cut(s) 19, 25
BtsCI GGATG 2 cut(s) 199, 217
BtsIMutI CAGTG 1 cut(s) 428
BveI ACCTGC 1 cut(s) 212
CaiI CAGNNNCTG 1 cut(s) 543
Cfr10I RCCGGY 3 cut(s) 339, 437, 484
Cfr13I GGNCC 3 cut(s) 357, 461, 534
CseI GACGC 1 cut(s) 55
CspAI ACCGGT 1 cut(s) 339
CviAII CATG 3 cut(s) 26, 31, 55
EaeI YGGCCR 1 cut(s) 22
Eco130I CCWWGG 3 cut(s) 25, 300, 530
Eco47I GGWCC 1 cut(s) 357
Eco57I CTGAAG 1 cut(s) 83
EcoRII CCWGG 2 cut(s) 402, 457
EcoT14I CCWWGG 3 cut(s) 25, 300, 530
ErhI CCWWGG 3 cut(s) 25, 300, 530
FaeI CATG 3 cut(s) 29, 34, 58
FaiI YATR 4 cut(s) 27, 32, 56, 152
FaqI GGGAC 1 cut(s) 74
FatI CATG 3 cut(s) 25, 30, 54
Fnu4HI GCNGC 3 cut(s) 51, 183, 541
FokI GGATG 2 cut(s) 206, 224
Fsp4HI GCNGC 3 cut(s) 51, 183, 541
FspBI CTAG 3 cut(s) 39, 291, 443
GluI GCNGC 3 cut(s) 51, 183, 541
GsaI CCCAGC 1 cut(s) 540
GsuI CTGGAG 1 cut(s) 386
HaeIII GGCC 4 cut(s) 24, 441, 463, 535
HapII CCGG 3 cut(s) 340, 438, 485
HgaI GACGC 1 cut(s) 55
Hin1II CATG 3 cut(s) 29, 34, 58
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 2 cut(s) 83, 407
HpaI GTTAAC 1 cut(s) 124
HpaII CCGG 3 cut(s) 340, 438, 485
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 4 cut(s) 91, 124, 274, 345
Hpy188I TCNGA 4 cut(s) 102, 211, 267, 357
Hpy188III TCNNGA 1 cut(s) 411
Hpy8I GTNNAC 4 cut(s) 91, 124, 274, 345
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 5 cut(s) 413, 480, 494, 508, 515
HpyCH4III ACNGT 2 cut(s) 75, 253
HpyCH4V TGCA 2 cut(s) 281, 476
Hsp92II CATG 3 cut(s) 29, 34, 58
KspAI GTTAAC 1 cut(s) 124
Lsp1109I GCAGC 2 cut(s) 37, 169
MaeI CTAG 3 cut(s) 39, 291, 443
MaeIII GTNAC 1 cut(s) 80
MboII GAAGA 6 cut(s) 127, 259, 503, 506, 509, 523
MfeI CAATTG 1 cut(s) 284
MhlI GDGCHC 1 cut(s) 203
MluCI AATT 3 cut(s) 241, 284, 375
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 245
MnlI CCTC 6 cut(s) 51, 96, 190, 252, 334, 370
MseI TTAA 3 cut(s) 8, 123, 312
MslI CAYNNNNRTG 1 cut(s) 24
MspA1I CMGCKG 1 cut(s) 540
MspI CCGG 3 cut(s) 340, 438, 485
MspR9I CCNGG 2 cut(s) 404, 459
MunI CAATTG 1 cut(s) 284
MvaI CCWGG 2 cut(s) 404, 459
NcoI CCATGG 1 cut(s) 25
NlaIII CATG 3 cut(s) 29, 34, 58
NlaIV GGNNCC 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 80
NspV TTCGAA 1 cut(s) 113
PaqCI CACCTGC 1 cut(s) 212
PfeI GAWTC 1 cut(s) 407
PflFI GACNNNGTC 1 cut(s) 65
PfoI TCCNGGA 1 cut(s) 402
PinAI ACCGGT 1 cut(s) 339
PkrI GCNGC 3 cut(s) 52, 184, 542
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp6I CCWGG 2 cut(s) 402, 457
PspFI CCCAGC 1 cut(s) 536
PspGI CCWGG 2 cut(s) 402, 457
PspN4I GGNNCC 1 cut(s) 202
PspPI GGNCC 3 cut(s) 357, 461, 534
PstI CTGCAG 1 cut(s) 478
PstNI CAGNNNCTG 1 cut(s) 543
PsyI GACNNNGTC 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 24
SaqAI TTAA 3 cut(s) 8, 123, 312
SatI GCNGC 3 cut(s) 51, 183, 541
Sau96I GGNCC 3 cut(s) 357, 461, 534
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 404, 459
SduI GDGCHC 1 cut(s) 203
SetI ASST 8 cut(s) 99, 107, 150, 206, 353, 362, 472, 526
SfcI CTRYAG 1 cut(s) 474
SfuI TTCGAA 1 cut(s) 113
SinI GGWCC 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 24
Sse9I AATT 3 cut(s) 241, 284, 375
SsiI CCGC 1 cut(s) 540
SspMI CTAG 3 cut(s) 39, 291, 443
StyD4I CCNGG 2 cut(s) 402, 457
StyI CCWWGG 3 cut(s) 25, 300, 530
TaaI ACNGT 2 cut(s) 75, 253
TaqI TCGA 2 cut(s) 69, 113
TasI AATT 3 cut(s) 241, 284, 375
TauI GCSGC 1 cut(s) 543
TfiI GAWTC 1 cut(s) 407
Tru1I TTAA 3 cut(s) 8, 123, 312
Tru9I TTAA 3 cut(s) 8, 123, 312
TscAI CASTG 1 cut(s) 435
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 2 cut(s) 50, 182
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 2 cut(s) 47, 468
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 1 cut(s) 435
Tth111I GACNNNGTC 1 cut(s) 65
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 2 cut(s) 241, 375
XspI CTAG 3 cut(s) 39, 291, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.