Rmu_ssc0000393.1_g000035

Coatomer subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000393.1
N/A
Physical Location & Seq
Forward (+)
197771 .. 203687
5917 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 999 bp
atgagcactatgcttcacaatgcccataccttttctctgtccctgagggcgcaactcttggacctggttggattttcgtataattgtgaaaagacggttcaaaaggagatctgtgagaattcgaccgttggatcatcgtataattttgtgaggatgattctaaaggcgatctgggatgatccaaccgttggatcttcgtataattgtgcaaagatgattcaaaaggcgatccgtaaggatccgaccgttggattgtcatataattttgtgaggatgattctaagggcgatccgggacgatccaaccgttgaattttcgtataattttgagaggatgaacctaagggctatccgtgaggatctgactgttggatcatggcctagacctgggtatgcattagcctgcaaggaatgttatttggtgggtggccaccctgaaagaccctgggaagcgtttgctgttagaaaggattcgagcccatctgtgaagaacattcttcttctagactctgaagggaagcgtgtggccgtcaaatattactctgatgattggccgacgaatgctgcaaagctagcttttgaaagatctgtgtttgctaagactctaaagacaaatgcaaggatagaagcggagataatgatgtttgagagccacgttgttacatataagtttgtgcaggaccttcacttctttgtgactggaggtgacgatgaaaacgaactcattttagccactgtacttcaggggttctttgatgcagttgcccttctcttgaggaacaacgtcgacaaaagagaggcactagagaacctggatctcatacttctatgctttgatgagatcgttgatggagggatgatacttgaaacagatccaactgttatcgcaggaaaggtggcaactcataccatggatgctgatgcaccattgtctgagcagactataacacaagcatgggctacagcaagggaacatctaaccagaacccttctaaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

332

Amino Acids

37.5

Weight (kDa)

5.6

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 162 - 301 2.8e-20 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 188
AccI GTMKAC 1 cut(s) 786
AciI CCGC 1 cut(s) 629
AcoI YGGCCR 3 cut(s) 427, 525, 551
AcsI RAATTY 2 cut(s) 118, 311
AcuI CTGAAG 2 cut(s) 531, 725
AfaI GTAC 1 cut(s) 738
AfiI CCNNNNNNNGG 3 cut(s) 188, 248, 386
AgsI TTSAA 5 cut(s) 101, 221, 311, 581, 866
AjnI CCWGG 4 cut(s) 63, 385, 443, 810
AluBI AGCT 2 cut(s) 571, 575
AluI AGCT 2 cut(s) 571, 575
Alw21I GWGCWC 1 cut(s) 8
AoxI GGCC 4 cut(s) 377, 427, 525, 551
ApeKI GCWGC 1 cut(s) 563
ApoI RAATTY 2 cut(s) 118, 311
Asp700I GAANNNNTTC 1 cut(s) 469
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 2 cut(s) 61, 679
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 716
AsuNHI GCTAGC 1 cut(s) 571
AvaII GGWCC 2 cut(s) 61, 679
AxyI CCTNAGG 2 cut(s) 44, 341
BalI TGGCCA 1 cut(s) 429
BamHI GGATCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 1 cut(s) 550
BccI CCATC 2 cut(s) 487, 842
BceAI ACGGC 1 cut(s) 512
BciT130I CCWGG 4 cut(s) 65, 387, 445, 812
BcnI CCSGG 1 cut(s) 293
BfaI CTAG 4 cut(s) 381, 503, 572, 803
BfmI CTRYAG 1 cut(s) 960
BglII AGATCT 2 cut(s) 108, 584
BisI GCNGC 1 cut(s) 564
BlsI GCNGC 1 cut(s) 565
Bme1390I CCNGG 5 cut(s) 65, 293, 387, 445, 812
Bme18I GGWCC 2 cut(s) 61, 679
BmgT120I GGNCC 2 cut(s) 61, 679
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 5 cut(s) 65, 293, 387, 445, 812
BmsI GCATC 3 cut(s) 745, 904, 910
BmtI GCTAGC 1 cut(s) 575
BpmI CTGGAG 1 cut(s) 720
BpuEI CTTGAG 1 cut(s) 793
BpuMI CCSGG 1 cut(s) 293
BsaJI CCNNGG 4 cut(s) 386, 443, 444, 909
Bsc4I CCNNNNNNNGG 3 cut(s) 188, 248, 386
Bse1I ACTGG 1 cut(s) 703
Bse21I CCTNAGG 2 cut(s) 44, 341
BseBI CCWGG 4 cut(s) 65, 387, 445, 812
BseDI CCNNGG 4 cut(s) 386, 443, 444, 909
BseGI GGATG 6 cut(s) 159, 181, 279, 339, 861, 919
BseLI CCNNNNNNNGG 3 cut(s) 188, 248, 386
BseMII CTCAG 2 cut(s) 35, 924
BseNI ACTGG 1 cut(s) 703
BseXI GCAGC 1 cut(s) 550
BsgI GTGCAG 1 cut(s) 695
Bsh1285I CGRYCG 2 cut(s) 126, 246
BshFI GGCC 4 cut(s) 379, 429, 527, 553
BsiEI CGRYCG 2 cut(s) 126, 246
BsiHKAI GWGCWC 1 cut(s) 8
BsiSI CCGG 1 cut(s) 292
BslFI GGGAC 2 cut(s) 25, 308
BslI CCNNNNNNNGG 3 cut(s) 188, 248, 386
BsmFI GGGAC 2 cut(s) 25, 308
BsmI GAATGC 1 cut(s) 565
BsnI GGCC 4 cut(s) 379, 429, 527, 553
Bsp1286I GDGCHC 2 cut(s) 8, 479
Bsp19I CCATGG 1 cut(s) 909
BspACI CCGC 1 cut(s) 629
BspANI GGCC 4 cut(s) 379, 429, 527, 553
BspCNI CTCAG 2 cut(s) 36, 925
BspLI GGNNCC 1 cut(s) 240
BspOI GCTAGC 1 cut(s) 575
BsrI ACTGG 1 cut(s) 703
BssECI CCNNGG 4 cut(s) 386, 443, 444, 909
BssT1I CCWWGG 1 cut(s) 909
Bst2UI CCWGG 4 cut(s) 65, 387, 445, 812
Bst4CI ACNGT 8 cut(s) 97, 127, 187, 247, 307, 367, 736, 880
BstC8I GCNNGC 2 cut(s) 403, 573
BstDEI CTNAG 5 cut(s) 44, 281, 341, 597, 933
BstDSI CCRYGG 1 cut(s) 909
BstF5I GGATG 6 cut(s) 159, 181, 279, 339, 861, 919
BstHHI GCGC 1 cut(s) 52
BstMCI CGRYCG 2 cut(s) 126, 246
BstMWI GCNNNNNNNGC 1 cut(s) 572
BstNI CCWGG 4 cut(s) 65, 387, 445, 812
BstSCI CCNGG 5 cut(s) 63, 291, 385, 443, 810
BstSFI CTRYAG 1 cut(s) 960
BstV1I GCAGC 1 cut(s) 550
BstX2I RGATCY 7 cut(s) 108, 191, 238, 358, 584, 814, 871
BstYI RGATCY 7 cut(s) 108, 191, 238, 358, 584, 814, 871
Bsu36I CCTNAGG 2 cut(s) 44, 341
BsuRI GGCC 4 cut(s) 379, 429, 527, 553
BtgI CCRYGG 1 cut(s) 909
BtsCI GGATG 6 cut(s) 159, 181, 279, 339, 861, 919
BtsIMutI CAGTG 1 cut(s) 732
Cac8I GCNNGC 2 cut(s) 403, 573
CfoI GCGC 1 cut(s) 52
Cfr13I GGNCC 2 cut(s) 61, 679
CsiI ACCWGGT 1 cut(s) 63
Csp6I GTAC 1 cut(s) 737
CviAII CATG 3 cut(s) 375, 910, 954
CviQI GTAC 1 cut(s) 737
DdeI CTNAG 5 cut(s) 44, 281, 341, 597, 933
EaeI YGGCCR 3 cut(s) 427, 525, 551
Eco130I CCWWGG 1 cut(s) 909
Eco24I GRGCYC 1 cut(s) 479
Eco47I GGWCC 2 cut(s) 61, 679
Eco57I CTGAAG 2 cut(s) 531, 725
Eco81I CCTNAGG 2 cut(s) 44, 341
EcoO109I RGGNCCY 1 cut(s) 679
EcoRI GAATTC 1 cut(s) 118
EcoRII CCWGG 4 cut(s) 63, 385, 443, 810
EcoT14I CCWWGG 1 cut(s) 909
EcoT22I ATGCAT 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 479
ErhI CCWWGG 1 cut(s) 909
FaeI CATG 3 cut(s) 378, 913, 957
FaqI GGGAC 2 cut(s) 25, 308
FatI CATG 3 cut(s) 374, 909, 953
FblI GTMKAC 1 cut(s) 786
Fnu4HI GCNGC 1 cut(s) 564
FokI GGATG 6 cut(s) 166, 188, 286, 346, 868, 926
FriOI GRGCYC 1 cut(s) 479
Fsp4HI GCNGC 1 cut(s) 564
FspBI CTAG 4 cut(s) 381, 503, 572, 803
GlaI GCGC 1 cut(s) 51
GluI GCNGC 1 cut(s) 564
GsuI CTGGAG 1 cut(s) 720
HaeIII GGCC 4 cut(s) 379, 429, 527, 553
HapII CCGG 1 cut(s) 292
HhaI GCGC 1 cut(s) 52
Hin1II CATG 3 cut(s) 378, 913, 957
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HincII GTYRAC 1 cut(s) 787
HindII GTYRAC 1 cut(s) 787
HinfI GANTC 6 cut(s) 157, 217, 277, 470, 506, 601
HpaII CCGG 1 cut(s) 292
HphI GGTGA 1 cut(s) 716
Hpy166II GTNNAC 1 cut(s) 787
Hpy188I TCNGA 5 cut(s) 243, 363, 511, 544, 934
Hpy188III TCNNGA 2 cut(s) 503, 772
Hpy8I GTNNAC 1 cut(s) 787
Hpy99I CGWCG 2 cut(s) 559, 788
HpyAV CCTTC 4 cut(s) 506, 692, 776, 998
HpyCH4III ACNGT 8 cut(s) 97, 127, 187, 247, 307, 367, 736, 880
HpyCH4IV ACGT 2 cut(s) 654, 783
HpyCH4V TGCA 8 cut(s) 209, 395, 405, 566, 617, 676, 758, 923
HpyF10VI GCNNNNNNNGC 1 cut(s) 572
HpyF3I CTNAG 5 cut(s) 44, 281, 341, 597, 933
HpySE526I ACGT 2 cut(s) 654, 783
Hsp92II CATG 3 cut(s) 378, 913, 957
HspAI GCGC 1 cut(s) 50
Lsp1109I GCAGC 1 cut(s) 550
LweI GCATC 3 cut(s) 745, 904, 910
MabI ACCWGGT 1 cut(s) 63
MaeI CTAG 4 cut(s) 381, 503, 572, 803
MaeII ACGT 2 cut(s) 654, 783
MaeIII GTNAC 3 cut(s) 658, 694, 704
MboII GAAGA 4 cut(s) 186, 488, 491, 499
MflI RGATCY 7 cut(s) 108, 191, 238, 358, 584, 814, 871
MhlI GDGCHC 2 cut(s) 8, 479
MlsI TGGCCA 1 cut(s) 429
MluCI AATT 7 cut(s) 82, 118, 142, 202, 262, 311, 322
MluNI TGGCCA 1 cut(s) 429
MlyI GAGTC 2 cut(s) 500, 595
MmeI TCCRAC 9 cut(s) 49, 109, 169, 206, 229, 266, 326, 349, 899
MnlI CCTC 9 cut(s) 39, 144, 264, 324, 349, 695, 768, 790, 845
Mox20I TGGCCA 1 cut(s) 429
Mph1103I ATGCAT 1 cut(s) 397
MroXI GAANNNNTTC 1 cut(s) 469
MscI TGGCCA 1 cut(s) 429
MslI CAYNNNNRTG 1 cut(s) 952
Msp20I TGGCCA 1 cut(s) 429
MspI CCGG 1 cut(s) 292
MspR9I CCNGG 5 cut(s) 65, 293, 387, 445, 812
Mva1269I GAATGC 1 cut(s) 565
MvaI CCWGG 4 cut(s) 65, 387, 445, 812
MwoI GCNNNNNNNGC 1 cut(s) 572
NciI CCSGG 1 cut(s) 293
NcoI CCATGG 1 cut(s) 909
NheI GCTAGC 1 cut(s) 571
NlaIII CATG 3 cut(s) 378, 913, 957
NlaIV GGNNCC 1 cut(s) 240
NmuCI GTSAC 2 cut(s) 694, 704
NsiI ATGCAT 1 cut(s) 397
PasI CCCWGGG 1 cut(s) 444
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 714
PctI GAATGC 1 cut(s) 565
PdmI GAANNNNTTC 1 cut(s) 469
PfeI GAWTC 4 cut(s) 157, 217, 277, 470
PflMI CCANNNNNTGG 1 cut(s) 188
PfoI TCCNGGA 1 cut(s) 291
PkrI GCNGC 1 cut(s) 565
PleI GAGTC 2 cut(s) 500, 595
PpsI GAGTC 2 cut(s) 500, 595
PpuMI RGGWCCY 1 cut(s) 679
Psp5II RGGWCCY 1 cut(s) 679
Psp6I CCWGG 4 cut(s) 63, 385, 443, 810
PspGI CCWGG 4 cut(s) 63, 385, 443, 810
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 2 cut(s) 61, 679
PspPPI RGGWCCY 1 cut(s) 679
PsuI RGATCY 7 cut(s) 108, 191, 238, 358, 584, 814, 871
RsaI GTAC 1 cut(s) 738
RsaNI GTAC 1 cut(s) 737
RseI CAYNNNNRTG 1 cut(s) 952
SalI GTCGAC 1 cut(s) 785
SatI GCNGC 1 cut(s) 564
Sau96I GGNCC 2 cut(s) 61, 679
SchI GAGTC 2 cut(s) 500, 595
ScrFI CCNGG 5 cut(s) 65, 293, 387, 445, 812
SduI GDGCHC 2 cut(s) 8, 479
SexAI ACCWGGT 1 cut(s) 63
SfaNI GCATC 3 cut(s) 745, 904, 910
SfcI CTRYAG 1 cut(s) 960
SinI GGWCC 2 cut(s) 61, 679
SmiMI CAYNNNNRTG 1 cut(s) 952
SmlI CTYRAG 1 cut(s) 772
SmoI CTYRAG 1 cut(s) 772
Sse9I AATT 7 cut(s) 82, 118, 142, 202, 262, 311, 322
SsiI CCGC 1 cut(s) 629
SspI AATATT 1 cut(s) 536
SspMI CTAG 4 cut(s) 381, 503, 572, 803
StyD4I CCNGG 5 cut(s) 63, 291, 385, 443, 810
StyI CCWWGG 1 cut(s) 909
TaaI ACNGT 8 cut(s) 97, 127, 187, 247, 307, 367, 736, 880
TaiI ACGT 2 cut(s) 657, 786
TaqI TCGA 3 cut(s) 122, 473, 786
TasI AATT 7 cut(s) 82, 118, 142, 202, 262, 311, 322
TatI WGTACW 1 cut(s) 736
TfiI GAWTC 4 cut(s) 157, 217, 277, 470
TscAI CASTG 1 cut(s) 739
TseFI GTSAC 2 cut(s) 694, 704
TseI GCWGC 1 cut(s) 563
Tsp45I GTSAC 2 cut(s) 694, 704
TspDTI ATGAA 2 cut(s) 350, 726
TspGWI ACGGA 2 cut(s) 221, 341
TspRI CASTG 1 cut(s) 739
Van91I CCANNNNNTGG 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 61, 679
XapI RAATTY 2 cut(s) 118, 311
XbaI TCTAGA 1 cut(s) 502
XmiI GTMKAC 1 cut(s) 786
XmnI GAANNNNTTC 1 cut(s) 469
XspI CTAG 4 cut(s) 381, 503, 572, 803
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.