Rmu_ssc0000398.1_g000016

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000398.1
N/A
Physical Location & Seq
Forward (+)
43047 .. 45812
2766 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 816 bp
atgatcttatggttgcaagttcatccaacgtcgttttgttcaacatggcccacacacacacacacacacacacacacacgaagccgttcagaagaggacgtccgcacagatgaaaaagtgtggacgtccctcctccggccacgattaagcggcgatgtcggtgctactgttgccgaacccgaacccgaaccaggcctcgatgttgagcttttgcttgcagctggagtcgtcgatgaagagttcgaagtcgacctcgatgagcttccatgccttcgccttgatgatgacgccgtccgggatgtccagagggtttgtccagcaatgacagctcctgaaaccgctgctagatcgagcatgttcgatgcaagagcaaccatgttgctcattggcttgcgcgtaaggattcgagctgtcaaggtggttcagactctaccagctcgacgcctaaactttgccgaacgttcggttcattatcgcgagcaaagagatcacaggtcgagagctgtacattattctgacccagaccaagctgtgtggaactctcgaattgaagaagctccggtgctcggaagaaggatcaagctctggaccgatttggttgggttgcttatgtgtttttggtttcaggccgaggcttgtatgaacgctcaggctttgaggaagtttgtggaggagaatcagagactagcggcagagtgtgcgcatctggtgagtcagtgtaacaagttggagaaggagtgccagagggccaaggaggctgagttggaggatgaactgggagaattgagggaagaattgaagttttacaaggaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

271

Amino Acids

31.17

Weight (kDa)

5.4

Isoelectric Point (pI)

56.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 102, 128
Acc16I TGCGCA 1 cut(s) 702
AccI GTMKAC 1 cut(s) 249
AccII CGCG 2 cut(s) 396, 477
AciI CCGC 4 cut(s) 103, 150, 339, 689
AclI AACGTT 1 cut(s) 460
AclWI GGATC 1 cut(s) 584
AcoI YGGCCR 1 cut(s) 137
AcyI GRCGYC 4 cut(s) 99, 125, 288, 442
AfaI GTAC 1 cut(s) 507
AfiI CCNNNNNNNGG 3 cut(s) 135, 191, 566
AgsI TTSAA 3 cut(s) 42, 551, 799
AjnI CCWGG 1 cut(s) 190
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 1 cut(s) 676
AlwI GGATC 1 cut(s) 584
AlwNI CAGNNNCTG 1 cut(s) 332
AoxI GGCC 5 cut(s) 47, 137, 193, 627, 747
ApeKI GCWGC 2 cut(s) 218, 341
Asp700I GAANNNNTTC 1 cut(s) 85
AspLEI GCGC 2 cut(s) 396, 703
AspS9I GGNCC 3 cut(s) 48, 588, 747
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 721
AsuII TTCGAA 1 cut(s) 243
AvaII GGWCC 1 cut(s) 588
Bbv12I GWGCWC 1 cut(s) 567
BbvI GCAGC 2 cut(s) 230, 328
BceAI ACGGC 2 cut(s) 69, 275
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 1 cut(s) 192
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 1 cut(s) 676
BfaI CTAG 2 cut(s) 345, 686
BglI GCCNNNNNGGC 1 cut(s) 755
BisI GCNGC 4 cut(s) 151, 219, 342, 690
BlsI GCNGC 4 cut(s) 152, 220, 343, 691
Bme1390I CCNGG 2 cut(s) 192, 296
Bme18I GGWCC 1 cut(s) 588
BmgT120I GGNCC 3 cut(s) 48, 588, 747
BmrFI CCNGG 2 cut(s) 192, 296
BmrI ACTGGG 1 cut(s) 785
BmsI GCATC 2 cut(s) 352, 712
BmuI ACTGGG 1 cut(s) 785
BpmI CTGGAG 1 cut(s) 243
Bpu10I CCTNAGC 1 cut(s) 648
Bpu14I TTCGAA 1 cut(s) 243
BpuMI CCSGG 1 cut(s) 296
BsaHI GRCGYC 4 cut(s) 99, 125, 288, 442
BsaJI CCNNGG 2 cut(s) 630, 750
BsaWI WCCGGW 1 cut(s) 559
BsaXI ACNNNNNCTCC 4 cut(s) 722, 746, 752, 776
Bsc4I CCNNNNNNNGG 3 cut(s) 135, 191, 566
Bse1I ACTGG 1 cut(s) 780
Bse3DI GCAATG 1 cut(s) 327
BseBI CCWGG 1 cut(s) 192
BseDI CCNNGG 2 cut(s) 630, 750
BseGI GGATG 3 cut(s) 22, 304, 775
BseLI CCNNNNNNNGG 3 cut(s) 135, 191, 566
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 2 cut(s) 662, 750
BseNI ACTGG 1 cut(s) 780
BseRI GAGGAG 2 cut(s) 122, 686
BseXI GCAGC 2 cut(s) 230, 328
Bsh1236I CGCG 2 cut(s) 396, 477
BshFI GGCC 5 cut(s) 49, 139, 195, 629, 749
BsiHKAI GWGCWC 1 cut(s) 567
BsiSI CCGG 3 cut(s) 136, 295, 560
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 3 cut(s) 135, 191, 566
BsmAI GTCTC 1 cut(s) 676
BsmFI GGGAC 1 cut(s) 112
BsnI GGCC 5 cut(s) 49, 139, 195, 629, 749
Bsp119I TTCGAA 1 cut(s) 243
Bsp1286I GDGCHC 1 cut(s) 567
Bsp1407I TGTACA 1 cut(s) 505
Bsp143I GATC 4 cut(s) 3, 347, 487, 576
Bsp68I TCGCGA 1 cut(s) 477
BspACI CCGC 4 cut(s) 103, 150, 339, 689
BspANI GGCC 5 cut(s) 49, 139, 195, 629, 749
BspCNI CTCAG 2 cut(s) 661, 751
BspFNI CGCG 2 cut(s) 396, 477
BspPI GGATC 1 cut(s) 584
BspT104I TTCGAA 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 327
BsrGI TGTACA 1 cut(s) 505
BsrI ACTGG 1 cut(s) 780
BssECI CCNNGG 2 cut(s) 630, 750
BssMI GATC 4 cut(s) 3, 347, 487, 576
BssNI GRCGYC 4 cut(s) 99, 125, 288, 442
BssT1I CCWWGG 1 cut(s) 750
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 1 cut(s) 169
Bst6I CTCTTC 2 cut(s) 87, 231
BstACI GRCGYC 4 cut(s) 99, 125, 288, 442
BstAPI GCANNNNNTGC 1 cut(s) 698
BstAUI TGTACA 1 cut(s) 505
BstBI TTCGAA 1 cut(s) 243
BstC8I GCNNGC 3 cut(s) 216, 392, 479
BstDEI CTNAG 2 cut(s) 648, 759
BstF5I GGATG 3 cut(s) 22, 304, 775
BstFNI CGCG 2 cut(s) 396, 477
BstHHI GCGC 2 cut(s) 396, 703
BstKTI GATC 4 cut(s) 6, 350, 490, 579
BstMAI GTCTC 1 cut(s) 676
BstMBI GATC 4 cut(s) 3, 347, 487, 576
BstMWI GCNNNNNNNGC 4 cut(s) 170, 326, 698, 755
BstNI CCWGG 1 cut(s) 192
BstNSI RCATGY 1 cut(s) 358
BstSCI CCNGG 2 cut(s) 190, 294
BstUI CGCG 2 cut(s) 396, 477
BstV1I GCAGC 2 cut(s) 230, 328
BsuRI GGCC 5 cut(s) 49, 139, 195, 629, 749
BtgZI GCGATG 1 cut(s) 168
BtsCI GGATG 3 cut(s) 22, 304, 775
BtsIMutI CAGTG 1 cut(s) 722
BtuMI TCGCGA 1 cut(s) 477
Cac8I GCNNGC 3 cut(s) 216, 392, 479
CaiI CAGNNNCTG 1 cut(s) 332
CfoI GCGC 2 cut(s) 396, 703
Cfr13I GGNCC 3 cut(s) 48, 588, 747
CseI GACGC 2 cut(s) 296, 450
Csp6I GTAC 1 cut(s) 506
CspCI CAANNNNNGTGG 2 cut(s) 515, 550
CviAII CATG 4 cut(s) 45, 267, 355, 376
CviQI GTAC 1 cut(s) 506
DdeI CTNAG 2 cut(s) 648, 759
DpnI GATC 4 cut(s) 5, 349, 489, 578
DpnII GATC 4 cut(s) 3, 347, 487, 576
EaeI YGGCCR 1 cut(s) 137
Eam1104I CTCTTC 2 cut(s) 87, 231
EarI CTCTTC 2 cut(s) 87, 231
Eco130I CCWWGG 1 cut(s) 750
Eco147I AGGCCT 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 588
EcoRII CCWGG 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 750
ErhI CCWWGG 1 cut(s) 750
FaeI CATG 4 cut(s) 48, 270, 358, 379
FaiI YATR 7 cut(s) 10, 46, 268, 356, 377, 611, 641
FaqI GGGAC 1 cut(s) 112
FatI CATG 4 cut(s) 44, 266, 354, 375
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 4 cut(s) 151, 219, 342, 690
FokI GGATG 3 cut(s) 9, 311, 782
Fsp4HI GCNGC 4 cut(s) 151, 219, 342, 690
FspAI RTGCGCAY 1 cut(s) 702
FspBI CTAG 2 cut(s) 345, 686
FspI TGCGCA 1 cut(s) 702
GlaI GCGC 2 cut(s) 395, 702
GluI GCNGC 4 cut(s) 151, 219, 342, 690
GsuI CTGGAG 1 cut(s) 243
HaeIII GGCC 5 cut(s) 49, 139, 195, 629, 749
HapII CCGG 3 cut(s) 136, 295, 560
HgaI GACGC 2 cut(s) 296, 450
HhaI GCGC 2 cut(s) 396, 703
Hin1I GRCGYC 4 cut(s) 99, 125, 288, 442
Hin1II CATG 4 cut(s) 48, 270, 358, 379
Hin6I GCGC 2 cut(s) 394, 701
HinP1I GCGC 2 cut(s) 394, 701
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 5 cut(s) 225, 403, 427, 676, 712
HpaII CCGG 3 cut(s) 136, 295, 560
HphI GGTGA 1 cut(s) 721
Hpy166II GTNNAC 2 cut(s) 123, 250
Hpy188I TCNGA 5 cut(s) 91, 426, 517, 569, 681
Hpy188III TCNNGA 6 cut(s) 304, 332, 476, 498, 543, 586
Hpy8I GTNNAC 2 cut(s) 123, 250
Hpy99I CGWCG 3 cut(s) 34, 233, 444
HpyAV CCTTC 3 cut(s) 281, 567, 727
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4IV ACGT 4 cut(s) 29, 99, 125, 460
HpyCH4V TGCA 3 cut(s) 16, 218, 365
HpyF10VI GCNNNNNNNGC 4 cut(s) 170, 326, 698, 755
HpyF3I CTNAG 2 cut(s) 648, 759
HpySE526I ACGT 4 cut(s) 29, 99, 125, 460
Hsp92I GRCGYC 4 cut(s) 99, 125, 288, 442
Hsp92II CATG 4 cut(s) 48, 270, 358, 379
HspAI GCGC 2 cut(s) 394, 701
Kzo9I GATC 4 cut(s) 3, 347, 487, 576
LmnI GCTCC 2 cut(s) 334, 562
Lsp1109I GCAGC 2 cut(s) 230, 328
LweI GCATC 2 cut(s) 352, 712
MaeI CTAG 2 cut(s) 345, 686
MaeII ACGT 4 cut(s) 29, 99, 125, 460
MaeIII GTNAC 1 cut(s) 719
MalI GATC 4 cut(s) 5, 349, 489, 578
MboI GATC 4 cut(s) 3, 347, 487, 576
MboII GAAGA 5 cut(s) 104, 248, 563, 582, 803
MhlI GDGCHC 1 cut(s) 567
MluCI AATT 3 cut(s) 546, 782, 794
MlyI GAGTC 3 cut(s) 234, 421, 721
MmeI TCCRAC 3 cut(s) 50, 708, 744
MroXI GAANNNNTTC 1 cut(s) 85
MseI TTAA 1 cut(s) 146
MspA1I CMGCKG 2 cut(s) 221, 341
MspI CCGG 3 cut(s) 136, 295, 560
MspR9I CCNGG 2 cut(s) 192, 296
MvaI CCWGG 1 cut(s) 192
MvnI CGCG 2 cut(s) 396, 477
MwoI GCNNNNNNNGC 4 cut(s) 170, 326, 698, 755
NciI CCSGG 1 cut(s) 296
NdeII GATC 4 cut(s) 3, 347, 487, 576
NlaIII CATG 4 cut(s) 48, 270, 358, 379
NmeAIII GCCGAG 1 cut(s) 655
NruI TCGCGA 1 cut(s) 477
NsbI TGCGCA 1 cut(s) 702
NspI RCATGY 1 cut(s) 358
NspV TTCGAA 1 cut(s) 243
PceI AGGCCT 1 cut(s) 195
PdmI GAANNNNTTC 1 cut(s) 85
PfeI GAWTC 2 cut(s) 403, 676
PflFI GACNNNGTC 1 cut(s) 290
PfoI TCCNGGA 1 cut(s) 294
PkrI GCNGC 4 cut(s) 152, 220, 343, 691
PleI GAGTC 3 cut(s) 233, 421, 720
PpsI GAGTC 3 cut(s) 233, 421, 720
Psp1406I AACGTT 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 190
PspGI CCWGG 1 cut(s) 190
PspPI GGNCC 3 cut(s) 48, 588, 747
PstNI CAGNNNCTG 1 cut(s) 332
PsyI GACNNNGTC 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 221
RruI TCGCGA 1 cut(s) 477
RsaI GTAC 1 cut(s) 507
RsaNI GTAC 1 cut(s) 506
SalI GTCGAC 1 cut(s) 248
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 4 cut(s) 151, 219, 342, 690
Sau3AI GATC 4 cut(s) 3, 347, 487, 576
Sau96I GGNCC 3 cut(s) 48, 588, 747
SchI GAGTC 3 cut(s) 234, 421, 721
ScrFI CCNGG 2 cut(s) 192, 296
SduI GDGCHC 1 cut(s) 567
SfaNI GCATC 2 cut(s) 352, 712
SfuI TTCGAA 1 cut(s) 243
SinI GGWCC 1 cut(s) 588
Sse9I AATT 3 cut(s) 546, 782, 794
SseBI AGGCCT 1 cut(s) 195
SsiI CCGC 4 cut(s) 103, 150, 339, 689
SspMI CTAG 2 cut(s) 345, 686
StuI AGGCCT 1 cut(s) 195
StyD4I CCNGG 2 cut(s) 190, 294
StyI CCWWGG 1 cut(s) 750
TaaI ACNGT 1 cut(s) 169
TaiI ACGT 4 cut(s) 32, 102, 128, 463
TaqII GACCGA 1 cut(s) 605
TasI AATT 3 cut(s) 546, 782, 794
TatI WGTACW 1 cut(s) 505
TauI GCSGC 2 cut(s) 153, 692
TfiI GAWTC 2 cut(s) 403, 676
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TscAI CASTG 1 cut(s) 722
TseI GCWGC 2 cut(s) 218, 341
TspDTI ATGAA 6 cut(s) 11, 126, 249, 458, 656, 786
TspRI CASTG 1 cut(s) 722
Tth111I GACNNNGTC 1 cut(s) 290
VpaK11BI GGWCC 1 cut(s) 588
XceI RCATGY 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 249
XmnI GAANNNNTTC 1 cut(s) 85
XspI CTAG 2 cut(s) 345, 686
ZraI GACGTC 2 cut(s) 100, 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.