Rmu_ssc0000416.1_g000007

Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000416.1
N/A
Physical Location & Seq
Reverse (-)
43696 .. 45398
1703 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 402 bp
atggctgctcaggctgtcgaaaacgtcgccgtatcggcgctccggtcggtcgttctccgagtccggcaggctgcggagcggtcgggccggaatccgggtcaggtgaggctggtagcggtgtcgaagacgaagcccatttctctcatccggcaagtctacgacgccggccaccggcgttttggggagaattacgtccaagaaatcctggagaaagctcctcagatgctatctaagtgtagaactgaggtatgcaaggagcttggtatggcagaggagaactttgagctgtcaatgggcatgtctggtgattttgagcaagcgattgaaatgggcagcaccaatgtcagagttggatccaccatctttgggccaagggagtatccaaagaagcaaccaaattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ala_racemase_N

Protein Analysis

133

Amino Acids

14.76

Weight (kDa)

9.3

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 79
AccI GTMKAC 1 cut(s) 156
AciI CCGC 3 cut(s) 74, 79, 116
AclWI GGATC 2 cut(s) 348, 361
AcoI YGGCCR 1 cut(s) 166
AcyI GRCGYC 1 cut(s) 162
AfiI CCNNNNNNNGG 3 cut(s) 94, 171, 366
AgsI TTSAA 1 cut(s) 326
AjnI CCWGG 1 cut(s) 204
AluBI AGCT 3 cut(s) 215, 259, 286
AluI AGCT 3 cut(s) 215, 259, 286
AlwI GGATC 2 cut(s) 348, 361
AoxI GGCC 3 cut(s) 85, 166, 368
ApeKI GCWGC 3 cut(s) 5, 71, 333
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 2 cut(s) 85, 368
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 2 cut(s) 115, 317
BamHI GGATCC 1 cut(s) 353
BbsI GAAGAC 1 cut(s) 131
BbvI GCAGC 2 cut(s) 58, 345
BccI CCATC 1 cut(s) 368
BceAI ACGGC 1 cut(s) 14
BciT130I CCWGG 1 cut(s) 206
BciVI GTATCC 1 cut(s) 390
BcnI CCSGG 1 cut(s) 96
BfoI RGCGCY 1 cut(s) 41
BfuI GTATCC 1 cut(s) 390
BglI GCCNNNNNGGC 1 cut(s) 35
BisI GCNGC 3 cut(s) 6, 72, 334
BlsI GCNGC 3 cut(s) 7, 73, 335
Bme1390I CCNGG 2 cut(s) 96, 206
BmgT120I GGNCC 2 cut(s) 85, 368
BmiI GGNNCC 1 cut(s) 355
BmrFI CCNGG 2 cut(s) 96, 206
BmsI GCATC 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 131
BpmI CTGGAG 1 cut(s) 227
Bpu10I CCTNAGC 1 cut(s) 9
BpuMI CCSGG 1 cut(s) 96
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 371
BsaWI WCCGGW 1 cut(s) 42
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 171, 366
Bse118I RCCGGY 2 cut(s) 164, 171
BseBI CCWGG 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 371
BseGI GGATG 1 cut(s) 144
BseLI CCNNNNNNNGG 3 cut(s) 94, 171, 366
BseMII CTCAG 3 cut(s) 23, 233, 234
BseRI GAGGAG 2 cut(s) 207, 287
BseXI GCAGC 2 cut(s) 58, 345
Bsh1285I CGRYCG 3 cut(s) 47, 51, 83
BshFI GGCC 3 cut(s) 87, 168, 370
BsiEI CGRYCG 3 cut(s) 47, 51, 83
BsiSI CCGG 7 cut(s) 43, 64, 88, 95, 148, 165, 172
BslI CCNNNNNNNGG 3 cut(s) 94, 171, 366
BsnI GGCC 3 cut(s) 87, 168, 370
Bsp143I GATC 1 cut(s) 353
BspACI CCGC 3 cut(s) 74, 79, 116
BspANI GGCC 3 cut(s) 87, 168, 370
BspCNI CTCAG 3 cut(s) 22, 232, 235
BspLI GGNNCC 1 cut(s) 355
BspPI GGATC 2 cut(s) 348, 361
BsrBI CCGCTC 1 cut(s) 79
BsrFI RCCGGY 2 cut(s) 164, 171
BssAI RCCGGY 2 cut(s) 164, 171
BssECI CCNNGG 1 cut(s) 371
BssMI GATC 1 cut(s) 353
BssNI GRCGYC 1 cut(s) 162
BssT1I CCWWGG 1 cut(s) 371
Bst2UI CCWGG 1 cut(s) 206
BstACI GRCGYC 1 cut(s) 162
BstC8I GCNNGC 3 cut(s) 69, 166, 318
BstDEI CTNAG 4 cut(s) 9, 219, 231, 243
BstF5I GGATG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 1 cut(s) 356
BstMBI GATC 1 cut(s) 353
BstMCI CGRYCG 3 cut(s) 47, 51, 83
BstMWI GCNNNNNNNGC 2 cut(s) 11, 35
BstNI CCWGG 1 cut(s) 206
BstNSI RCATGY 1 cut(s) 301
BstSCI CCNGG 2 cut(s) 94, 204
BstV1I GCAGC 2 cut(s) 58, 345
BstV2I GAAGAC 1 cut(s) 131
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 3 cut(s) 87, 168, 370
BtsCI GGATG 1 cut(s) 144
Cac8I GCNNGC 3 cut(s) 69, 166, 318
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 2 cut(s) 164, 171
Cfr13I GGNCC 2 cut(s) 85, 368
CseI GACGC 1 cut(s) 170
CspCI CAANNNNNGTGG 2 cut(s) 346, 381
CviAII CATG 1 cut(s) 298
DdeI CTNAG 4 cut(s) 9, 219, 231, 243
DpnI GATC 1 cut(s) 355
DpnII GATC 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 371
EcoRII CCWGG 1 cut(s) 204
EcoT14I CCWWGG 1 cut(s) 371
ErhI CCWWGG 1 cut(s) 371
FaeI CATG 1 cut(s) 301
FaiI YATR 3 cut(s) 250, 266, 299
FatI CATG 1 cut(s) 297
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 3 cut(s) 6, 72, 334
FokI GGATG 1 cut(s) 131
Fsp4HI GCNGC 3 cut(s) 6, 72, 334
GlaI GCGC 1 cut(s) 39
GluI GCNGC 3 cut(s) 6, 72, 334
GsuI CTGGAG 1 cut(s) 227
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 3 cut(s) 87, 168, 370
HapII CCGG 7 cut(s) 43, 64, 88, 95, 148, 165, 172
HgaI GACGC 1 cut(s) 170
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 1 cut(s) 301
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HinfI GANTC 2 cut(s) 60, 91
HpaII CCGG 7 cut(s) 43, 64, 88, 95, 148, 165, 172
HphI GGTGA 2 cut(s) 115, 317
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 3 cut(s) 59, 222, 347
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 2 cut(s) 29, 164
HpyCH4IV ACGT 2 cut(s) 24, 192
HpyCH4V TGCA 1 cut(s) 252
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 35
HpyF3I CTNAG 4 cut(s) 9, 219, 231, 243
HpySE526I ACGT 2 cut(s) 24, 192
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 1 cut(s) 301
HspAI GCGC 1 cut(s) 38
KroI GCCGGC 1 cut(s) 164
KroNI GCCGGC 1 cut(s) 166
Kzo9I GATC 1 cut(s) 353
LmnI GCTCC 4 cut(s) 45, 76, 220, 256
Lsp1109I GCAGC 2 cut(s) 58, 345
LweI GCATC 1 cut(s) 213
MaeII ACGT 2 cut(s) 24, 192
MalI GATC 1 cut(s) 355
MbiI CCGCTC 1 cut(s) 79
MboI GATC 1 cut(s) 353
MboII GAAGA 1 cut(s) 136
MflI RGATCY 1 cut(s) 353
MluCI AATT 2 cut(s) 187, 397
MlyI GAGTC 1 cut(s) 69
MmeI TCCRAC 1 cut(s) 331
MnlI CCTC 4 cut(s) 99, 228, 238, 265
MroNI GCCGGC 1 cut(s) 164
MseI TTAA 1 cut(s) 400
MspI CCGG 7 cut(s) 43, 64, 88, 95, 148, 165, 172
MspR9I CCNGG 2 cut(s) 96, 206
MvaI CCWGG 1 cut(s) 206
MwoI GCNNNNNNNGC 2 cut(s) 11, 35
NaeI GCCGGC 1 cut(s) 166
NciI CCSGG 1 cut(s) 96
NdeII GATC 1 cut(s) 353
NgoMIV GCCGGC 1 cut(s) 164
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 1 cut(s) 355
NspI RCATGY 1 cut(s) 301
PdiI GCCGGC 1 cut(s) 166
PfeI GAWTC 1 cut(s) 91
PfoI TCCNGGA 1 cut(s) 204
PkrI GCNGC 3 cut(s) 7, 73, 335
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
Psp6I CCWGG 1 cut(s) 204
PspGI CCWGG 1 cut(s) 204
PspN4I GGNNCC 1 cut(s) 355
PspPI GGNCC 2 cut(s) 85, 368
PsuI RGATCY 1 cut(s) 353
SaqAI TTAA 1 cut(s) 400
SatI GCNGC 3 cut(s) 6, 72, 334
Sau3AI GATC 1 cut(s) 353
Sau96I GGNCC 2 cut(s) 85, 368
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 2 cut(s) 96, 206
SetI ASST 7 cut(s) 27, 105, 195, 217, 249, 261, 288
SfaNI GCATC 1 cut(s) 213
SgrAI CRCCGGYG 1 cut(s) 171
Sse9I AATT 2 cut(s) 187, 397
SsiI CCGC 3 cut(s) 74, 79, 116
StyD4I CCNGG 2 cut(s) 94, 204
StyI CCWWGG 1 cut(s) 371
TaiI ACGT 2 cut(s) 27, 195
TaqI TCGA 2 cut(s) 18, 122
TaqII GACCGA 1 cut(s) 37
TasI AATT 2 cut(s) 187, 397
TfiI GAWTC 1 cut(s) 91
Tru1I TTAA 1 cut(s) 400
Tru9I TTAA 1 cut(s) 400
TseI GCWGC 3 cut(s) 5, 71, 333
XceI RCATGY 1 cut(s) 301
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.