Rmu_ssc0000432.1_g000025

EVI5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000432.1
N/A
Physical Location & Seq
Reverse (-)
102311 .. 107120
4810 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1566 bp
atgagaaatcacataaagatgtggaatttgataagagaaaagaaaagaaaaacccagaatgagggaatggaacctggaactgaagagttttctccaacacttggaccagactatgtagcatttggatgggcatggtttgggtcactttgggccaacacctctttagaagcgggttgggaaggagggttcgagcttactttgttattgaatcttattccgaccgagactcatgcacaaaacggcgccgttctccccctcctcttctgttcgttgattcccatcaccctgaggacgaagcactttaacatcttaccacgcgtaaaacctccatttggtttccgtgaacgattccaactaccattcgtaggccctcctctgtctcaatctaattcaggattgcagaatttccagagcaatcttatctcacattatcgaatcccaggaccaaaacggacaacaacccatcattcgtcgactcaaattctgcaaaacccagatttagctgtcttaagtgatcgtggagatcgtgagctgggtccagttcctccactgagattggacagatttgggtttttgaagcaggaaccatctcctaacggcttacccaagggcaggtcagcctttgaattcgagagggaggaaaggagggttaggaaatggaggaagatgattggagttggaggcagtgattggaaatattatcttaggagaaaacctcatgttgtcaaaaggagaataagaaaaggaattcctgattgtttaagaggtctggtatggcagttgatctctggaagtcgggatcttttgttgatgaaccctggtgtttatgagcaattggtaatttatgagacatcagcttcagagctggatataattcgagatatatcgcgtaccttcccttcacatgttttcttccagcagagacatggtcctggtcagaggtctctctacaatgttttgaaggcatactctgtctttgacagagatgttggatatgttcagggaatggggtttctggctggtctattgcttctttatatgagcgaggaggatgcattttggttattggtcgcattactcaaaggagcagtccatgcaccaatggaaggattatatcaggtaggtctaccactggtacaacaataccttttccagtttgaccagttggtaagggagcagttgccaaagctcggagagcattttacccaagaaatgataaatcccagcatgtatgcaagccagtggttcattacagttttttcttattcttttcctttccatttggctcttcgaatttgggatgtctttctctatgagggtgttaaaatcgtttttaaagttggtttggccctattaaaatattgccacgacgacttggtaaaattacccttcgagaaacttatccatgctttgcgtaacttccccgaggatgcaatggaccctgacacgttacttccaatggcttactctatcaaggtatccaagtgcttggaggaattgaaacagcagtatgataagaagaatgggaagaaccttaagcctacagaggatgagaaaccggcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RabGAP-TBC

Protein Analysis

521

Amino Acids

60.07

Weight (kDa)

9.25

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RabGAP-TBC PF00566 252 - 459 4.7e-60 Rab-GTPase-TBC domain
RabGap-TBC_2 PF23436 314 - 472 3.4e-45 RabGAP TBC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 603
AccB1I GGYRCC 1 cut(s) 242
AccB7I CCANNNNNTGG 1 cut(s) 101
AccI GTMKAC 2 cut(s) 473, 1126
AccII CGCG 2 cut(s) 318, 889
AciI CCGC 1 cut(s) 170
AclWI GGATC 1 cut(s) 807
AcsI RAATTY 6 cut(s) 25, 403, 480, 626, 747, 1293
AcuI CTGAAG 2 cut(s) 102, 843
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 2 cut(s) 892, 1137
AfiI CCNNNNNNNGG 7 cut(s) 61, 101, 332, 365, 612, 1106, 1190
AflII CTTAAG 2 cut(s) 508, 1535
AflIII ACRYGT 3 cut(s) 316, 904, 1446
AgsI TTSAA 5 cut(s) 208, 577, 626, 961, 1501
AjnI CCWGG 4 cut(s) 73, 439, 817, 931
AloI GAACNNNNNNTCC 2 cut(s) 170, 202
AluBI AGCT 6 cut(s) 193, 503, 532, 857, 865, 1189
AluI AGCT 6 cut(s) 193, 503, 532, 857, 865, 1189
Alw26I GTCTC 5 cut(s) 218, 384, 842, 916, 948
AlwI GGATC 1 cut(s) 807
Ama87I CYCGRG 1 cut(s) 1424
AoxI GGCC 3 cut(s) 150, 367, 1347
ApoI RAATTY 6 cut(s) 25, 403, 480, 626, 747, 1293
ArsI GACNNNNNNTTYG 2 cut(s) 1074, 1106
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 8 cut(s) 104, 150, 368, 443, 536, 929, 1348, 1438
AsuHPI GGTGA 1 cut(s) 274
AsuII TTCGAA 1 cut(s) 1291
AvaI CYCGRG 1 cut(s) 1424
AvaII GGWCC 5 cut(s) 104, 443, 536, 929, 1438
AxyI CCTNAGG 1 cut(s) 287
BanI GGYRCC 1 cut(s) 242
BarI GAAGNNNNNNTAC 2 cut(s) 883, 915
BccI CCATC 4 cut(s) 120, 287, 471, 595
BceAI ACGGC 3 cut(s) 230, 256, 613
BcgI CGANNNNNNTGC 4 cut(s) 212, 246, 1034, 1068
BciT130I CCWGG 4 cut(s) 75, 441, 819, 933
BciVI GTATCC 1 cut(s) 1489
BcoDI GTCTC 5 cut(s) 218, 384, 842, 916, 948
BfmI CTRYAG 1 cut(s) 1542
BfoI RGCGCY 1 cut(s) 246
BfrI CTTAAG 2 cut(s) 508, 1535
BfuAI ACCTGC 1 cut(s) 603
BfuI GTATCC 1 cut(s) 1489
Bme1390I CCNGG 4 cut(s) 75, 441, 819, 933
Bme18I GGWCC 5 cut(s) 104, 443, 536, 929, 1438
BmeT110I CYCGRG 1 cut(s) 1424
BmgT120I GGNCC 8 cut(s) 104, 150, 368, 443, 536, 929, 1348, 1438
BmiI GGNNCC 5 cut(s) 72, 244, 537, 585, 1440
BmrFI CCNGG 4 cut(s) 75, 441, 819, 933
BmsI GCATC 2 cut(s) 1042, 1420
BplI GAGNNNNNCTC 2 cut(s) 700, 732
Bpu14I TTCGAA 1 cut(s) 1291
BsaBI GATNNNNATC 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 948
BsaJI CCNNGG 4 cut(s) 439, 606, 817, 1425
Bsc4I CCNNNNNNNGG 7 cut(s) 61, 101, 332, 365, 612, 1106, 1190
Bse118I RCCGGY 1 cut(s) 1558
Bse1I ACTGG 5 cut(s) 539, 1137, 1153, 1162, 1240
Bse21I CCTNAGG 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 1440
Bse8I GATNNNNATC 1 cut(s) 278
BseBI CCWGG 4 cut(s) 75, 441, 819, 933
BseDI CCNNGG 4 cut(s) 439, 606, 817, 1425
BseGI GGATG 5 cut(s) 131, 1057, 1306, 1435, 1555
BseJI GATNNNNATC 1 cut(s) 278
BseLI CCNNNNNNNGG 7 cut(s) 61, 101, 332, 365, 612, 1106, 1190
BseMI GCAATG 1 cut(s) 1440
BseMII CTCAG 2 cut(s) 278, 542
BseNI ACTGG 5 cut(s) 539, 1137, 1153, 1162, 1240
BseRI GAGGAG 3 cut(s) 248, 363, 1061
BseYI CCCAGC 2 cut(s) 532, 1223
Bsh1236I CGCG 2 cut(s) 318, 889
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 3 cut(s) 152, 369, 1349
BshNI GGYRCC 1 cut(s) 242
BsiEI CGRYCG 1 cut(s) 222
BsiHKCI CYCGRG 1 cut(s) 1424
BsiSI CCGG 1 cut(s) 1559
BslI CCNNNNNNNGG 7 cut(s) 61, 101, 332, 365, 612, 1106, 1190
BsmAI GTCTC 5 cut(s) 218, 384, 842, 916, 948
BsnI GGCC 3 cut(s) 152, 369, 1349
Bso31I GGTCTC 1 cut(s) 948
BsoBI CYCGRG 1 cut(s) 1424
Bsp119I TTCGAA 1 cut(s) 1291
Bsp143I GATC 4 cut(s) 514, 523, 783, 799
BspACI CCGC 1 cut(s) 170
BspANI GGCC 3 cut(s) 152, 369, 1349
BspCNI CTCAG 2 cut(s) 279, 543
BspFNI CGCG 2 cut(s) 318, 889
BspLI GGNNCC 5 cut(s) 72, 244, 537, 585, 1440
BspMI ACCTGC 1 cut(s) 603
BspPI GGATC 1 cut(s) 807
BspQI GCTCTTC 1 cut(s) 1293
BspT104I TTCGAA 1 cut(s) 1291
BspT107I GGYRCC 1 cut(s) 242
BspTI CTTAAG 2 cut(s) 508, 1535
BspTNI GGTCTC 1 cut(s) 948
BsrDI GCAATG 1 cut(s) 1440
BsrFI RCCGGY 1 cut(s) 1558
BsrI ACTGG 5 cut(s) 539, 1137, 1153, 1162, 1240
BssAI RCCGGY 1 cut(s) 1558
BssECI CCNNGG 4 cut(s) 439, 606, 817, 1425
BssMI GATC 4 cut(s) 514, 523, 783, 799
BssNI GRCGYC 1 cut(s) 243
BssT1I CCWWGG 1 cut(s) 606
Bst2UI CCWGG 4 cut(s) 75, 441, 819, 933
Bst4CI ACNGT 1 cut(s) 1255
Bst6I CTCTTC 3 cut(s) 78, 266, 1293
BstACI GRCGYC 1 cut(s) 243
BstAFI CTTAAG 2 cut(s) 508, 1535
BstAPI GCANNNNNTGC 1 cut(s) 1094
BstBI TTCGAA 1 cut(s) 1291
BstC8I GCNNGC 1 cut(s) 1237
BstDEI CTNAG 3 cut(s) 287, 551, 704
BstF5I GGATG 5 cut(s) 131, 1057, 1306, 1435, 1555
BstFNI CGCG 2 cut(s) 318, 889
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 4 cut(s) 517, 526, 786, 802
BstMAI GTCTC 5 cut(s) 218, 384, 842, 916, 948
BstMBI GATC 4 cut(s) 514, 523, 783, 799
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 2 cut(s) 1094, 1195
BstNI CCWGG 4 cut(s) 75, 441, 819, 933
BstNSI RCATGY 2 cut(s) 908, 1231
BstSCI CCNGG 4 cut(s) 73, 439, 817, 931
BstSFI CTRYAG 1 cut(s) 1542
BstUI CGCG 2 cut(s) 318, 889
BstX2I RGATCY 1 cut(s) 799
BstXI CCANNNNNNTGG 1 cut(s) 1489
BstYI RGATCY 1 cut(s) 799
Bsu36I CCTNAGG 1 cut(s) 287
BsuI GTATCC 1 cut(s) 1489
BsuRI GGCC 3 cut(s) 152, 369, 1349
BtsCI GGATG 5 cut(s) 131, 1057, 1306, 1435, 1555
BtsI GCAGTG 1 cut(s) 691
BtsIMutI CAGTG 4 cut(s) 548, 691, 1130, 1247
BveI ACCTGC 1 cut(s) 603
Cac8I GCNNGC 1 cut(s) 1237
CfoI GCGC 1 cut(s) 245
Cfr10I RCCGGY 1 cut(s) 1558
Cfr13I GGNCC 8 cut(s) 104, 150, 368, 443, 536, 929, 1348, 1438
Csp6I GTAC 2 cut(s) 891, 1136
CspCI CAANNNNNGTGG 2 cut(s) 537, 572
CviAII CATG 8 cut(s) 132, 230, 719, 905, 926, 1094, 1228, 1406
CviQI GTAC 2 cut(s) 891, 1136
DdeI CTNAG 3 cut(s) 287, 551, 704
DinI GGCGCC 1 cut(s) 244
DpnI GATC 4 cut(s) 516, 525, 785, 801
DpnII GATC 4 cut(s) 514, 523, 783, 799
DraI TTTAAA 1 cut(s) 1336
Eam1104I CTCTTC 3 cut(s) 78, 266, 1293
EarI CTCTTC 3 cut(s) 78, 266, 1293
Eco130I CCWWGG 1 cut(s) 606
Eco31I GGTCTC 1 cut(s) 948
Eco47I GGWCC 5 cut(s) 104, 443, 536, 929, 1438
Eco57I CTGAAG 2 cut(s) 102, 843
Eco81I CCTNAGG 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 1424
EcoO109I RGGNCCY 1 cut(s) 368
EcoRI GAATTC 2 cut(s) 626, 747
EcoRII CCWGG 4 cut(s) 73, 439, 817, 931
EcoT14I CCWWGG 1 cut(s) 606
EcoT22I ATGCAT 1 cut(s) 1057
EgeI GGCGCC 1 cut(s) 244
EheI GGCGCC 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 8 cut(s) 135, 233, 722, 908, 929, 1097, 1231, 1409
FatI CATG 8 cut(s) 131, 229, 718, 904, 925, 1093, 1227, 1405
FauI CCCGC 1 cut(s) 163
FblI GTMKAC 2 cut(s) 473, 1126
FokI GGATG 5 cut(s) 138, 1064, 1313, 1442, 1562
GlaI GCGC 1 cut(s) 244
GsaI CCCAGC 2 cut(s) 536, 1227
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 3 cut(s) 152, 369, 1349
HapII CCGG 1 cut(s) 1559
HhaI GCGC 1 cut(s) 245
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 8 cut(s) 135, 233, 722, 908, 929, 1097, 1231, 1409
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HincII GTYRAC 1 cut(s) 474
HindII GTYRAC 1 cut(s) 474
HinfI GANTC 6 cut(s) 208, 226, 274, 348, 435, 475
HpaII CCGG 1 cut(s) 1559
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 3 cut(s) 344, 474, 1127
Hpy188I TCNGA 4 cut(s) 219, 862, 939, 1193
Hpy188III TCNNGA 9 cut(s) 393, 409, 527, 631, 752, 789, 797, 878, 1393
Hpy8I GTNNAC 3 cut(s) 344, 474, 1127
Hpy99I CGWCG 2 cut(s) 475, 1373
HpyAV CCTTC 6 cut(s) 173, 904, 909, 955, 1100, 1399
HpyCH4III ACNGT 1 cut(s) 1255
HpyCH4IV ACGT 1 cut(s) 1448
HpyCH4V TGCA 7 cut(s) 233, 400, 487, 1055, 1097, 1235, 1433
HpyF10VI GCNNNNNNNGC 2 cut(s) 1094, 1195
HpyF3I CTNAG 3 cut(s) 287, 551, 704
HpySE526I ACGT 1 cut(s) 1448
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 8 cut(s) 135, 233, 722, 908, 929, 1097, 1231, 1409
HspAI GCGC 1 cut(s) 243
KasI GGCGCC 1 cut(s) 242
Kzo9I GATC 4 cut(s) 514, 523, 783, 799
LguI GCTCTTC 1 cut(s) 1293
LmnI GCTCC 2 cut(s) 1085, 1174
LweI GCATC 2 cut(s) 1042, 1420
MaeII ACGT 1 cut(s) 1448
MaeIII GTNAC 3 cut(s) 141, 1415, 1449
MalI GATC 4 cut(s) 516, 525, 785, 801
MboI GATC 4 cut(s) 514, 523, 783, 799
MboII GAAGA 7 cut(s) 95, 253, 676, 904, 1280, 1531, 1540
MfeI CAATTG 1 cut(s) 833
MflI RGATCY 1 cut(s) 799
MluI ACGCGT 1 cut(s) 316
Mly113I GGCGCC 1 cut(s) 243
MlyI GAGTC 2 cut(s) 220, 469
MmeI TCCRAC 5 cut(s) 119, 242, 376, 658, 970
Mph1103I ATGCAT 1 cut(s) 1057
MseI TTAA 8 cut(s) 303, 509, 761, 1323, 1335, 1355, 1536, 1564
MslI CAYNNNNRTG 2 cut(s) 17, 124
MspCI CTTAAG 2 cut(s) 508, 1535
MspI CCGG 1 cut(s) 1559
MspR9I CCNGG 4 cut(s) 75, 441, 819, 933
MunI CAATTG 1 cut(s) 833
MvaI CCWGG 4 cut(s) 75, 441, 819, 933
MvnI CGCG 2 cut(s) 318, 889
MwoI GCNNNNNNNGC 2 cut(s) 1094, 1195
NarI GGCGCC 1 cut(s) 243
NdeII GATC 4 cut(s) 514, 523, 783, 799
NlaIII CATG 8 cut(s) 135, 233, 722, 908, 929, 1097, 1231, 1409
NlaIV GGNNCC 5 cut(s) 72, 244, 537, 585, 1440
NmuCI GTSAC 1 cut(s) 141
NsiI ATGCAT 1 cut(s) 1057
NspI RCATGY 2 cut(s) 908, 1231
NspV TTCGAA 1 cut(s) 1291
PciI ACATGT 1 cut(s) 904
PciSI GCTCTTC 1 cut(s) 1293
PcsI WCGNNNNNNNCGW 1 cut(s) 523
PfeI GAWTC 4 cut(s) 208, 274, 348, 435
PflFI GACNNNGTC 1 cut(s) 927
PflMI CCANNNNNTGG 1 cut(s) 101
PleI GAGTC 2 cut(s) 220, 469
PluTI GGCGCC 1 cut(s) 246
PpsI GAGTC 2 cut(s) 220, 469
PscI ACATGT 1 cut(s) 904
Psp6I CCWGG 4 cut(s) 73, 439, 817, 931
PspFI CCCAGC 2 cut(s) 532, 1223
PspGI CCWGG 4 cut(s) 73, 439, 817, 931
PspN4I GGNNCC 5 cut(s) 72, 244, 537, 585, 1440
PspPI GGNCC 8 cut(s) 104, 150, 368, 443, 536, 929, 1348, 1438
PsuI RGATCY 1 cut(s) 799
PsyI GACNNNGTC 1 cut(s) 927
RsaI GTAC 2 cut(s) 892, 1137
RsaNI GTAC 2 cut(s) 891, 1136
RseI CAYNNNNRTG 2 cut(s) 17, 124
SalI GTCGAC 1 cut(s) 472
SapI GCTCTTC 1 cut(s) 1293
SaqAI TTAA 8 cut(s) 303, 509, 761, 1323, 1335, 1355, 1536, 1564
Sau3AI GATC 4 cut(s) 514, 523, 783, 799
Sau96I GGNCC 8 cut(s) 104, 150, 368, 443, 536, 929, 1348, 1438
SchI GAGTC 2 cut(s) 220, 469
ScrFI CCNGG 4 cut(s) 75, 441, 819, 933
SfaNI GCATC 2 cut(s) 1042, 1420
SfcI CTRYAG 1 cut(s) 1542
SfoI GGCGCC 1 cut(s) 244
SfuI TTCGAA 1 cut(s) 1291
SinI GGWCC 5 cut(s) 104, 443, 536, 929, 1438
SmiMI CAYNNNNRTG 2 cut(s) 17, 124
SmlI CTYRAG 2 cut(s) 508, 1535
SmoI CTYRAG 2 cut(s) 508, 1535
SsiI CCGC 1 cut(s) 170
SspDI GGCGCC 1 cut(s) 242
SspI AATATT 2 cut(s) 698, 1361
StyD4I CCNGG 4 cut(s) 73, 439, 817, 931
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 1 cut(s) 1255
TaiI ACGT 1 cut(s) 1451
TaqI TCGA 7 cut(s) 189, 433, 473, 630, 877, 1291, 1392
TaqII GACCGA 1 cut(s) 236
TfiI GAWTC 4 cut(s) 208, 274, 348, 435
Tru1I TTAA 8 cut(s) 303, 509, 761, 1323, 1335, 1355, 1536, 1564
Tru9I TTAA 8 cut(s) 303, 509, 761, 1323, 1335, 1355, 1536, 1564
TscAI CASTG 4 cut(s) 555, 691, 1137, 1247
TseFI GTSAC 1 cut(s) 141
Tsp45I GTSAC 1 cut(s) 141
TspDTI ATGAA 2 cut(s) 827, 1237
TspGWI ACGGA 2 cut(s) 329, 466
TspRI CASTG 4 cut(s) 555, 691, 1137, 1247
Tth111I GACNNNGTC 1 cut(s) 927
Van91I CCANNNNNTGG 1 cut(s) 101
Vha464I CTTAAG 2 cut(s) 508, 1535
VpaK11BI GGWCC 5 cut(s) 104, 443, 536, 929, 1438
XapI RAATTY 6 cut(s) 25, 403, 480, 626, 747, 1293
XceI RCATGY 2 cut(s) 908, 1231
XcmI CCANNNNNNNNNTGG 1 cut(s) 923
XmiI GTMKAC 2 cut(s) 473, 1126
Zsp2I ATGCAT 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.