Rmu_ssc0000465.1_g000013

Short-chain dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000465.1
N/A
Physical Location & Seq
Forward (+)
45276 .. 48401
3126 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1011 bp
atggggatattcagcaagaaaggagcatcagggttctcggcctcttccacagcagaacaagttactcaagggattgatggaactggtcttacttcaattgttacaggagcctcaagtggtattggcgtagagacggcgcgtgttcttgcattgcgcggtgttcatgtaataatggcagtaaggaatatagatgctggtaccaatgttaaagaagcaatccttaaagaaatccccagtgctaaaattgatgtcatggagttagatctcagctcattggcatccgtaagaaaatttgcagcagagtataattccaaaggccttcccttgaatatccttattaacaatgcaggggtcatggcagataaattcaagctatctcaagacaacatagaactcggttttgcaactaaccatttaggtctgttaccttcaaattttgcaggctgctttaataaatctatacatttgttacttaaaccattaaattcaaagtcttggatggccacttggcagcatcaagatggctttgctgccgttatcaagactcatcttagcaacgcaatcaacaccaatgcctttgaatatgactccatggccactgaactcttgttccaggtagtccccgttgatggatacaacaaatatgttgcttacatagaatccaagctcgccaacatattgcatgctaatgagcttacaaggcgcctcaaggaagaaagggttgagataactgctaattctcttcatcctggaacaattcgcaccaatattatgcataatgatatcagtgtaaagtgtcttctgaaggtgctcaggatcctttttttgagcaaaactattcagcagggagcagcaaccacatgctttttggcactgaatccacaagttaagggagtaagcggtgaatactatgcggattgtaacattgccaaaccgatatctcaggcaaatgatgcagatttggctaagagactctgggattttagcttgagtttgaccaatctcaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
adh_short

Protein Analysis

336

Amino Acids

36.44

Weight (kDa)

8.75

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KR PF08659 32 - 123 1.5e-07 KR domain
adh_short PF00106 33 - 141 1.1e-16 short chain dehydrogenase
adh_short_C2 PF13561 36 - 131 2.9e-09 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 197
AccB1I GGYRCC 2 cut(s) 197, 702
AccII CGCG 2 cut(s) 139, 156
AciI CCGC 3 cut(s) 156, 900, 914
AclWI GGATC 2 cut(s) 811, 824
AcoI YGGCCR 2 cut(s) 501, 594
AcsI RAATTY 4 cut(s) 290, 365, 433, 484
AcuI CTGAAG 1 cut(s) 824
AcyI GRCGYC 1 cut(s) 703
AfaI GTAC 1 cut(s) 199
AfiI CCNNNNNNNGG 1 cut(s) 629
AgsI TTSAA 6 cut(s) 96, 328, 370, 432, 489, 581
AjnI CCWGG 2 cut(s) 612, 748
AloI GAACNNNNNNTCC 2 cut(s) 594, 626
AluBI AGCT 5 cut(s) 270, 373, 667, 694, 987
AluI AGCT 5 cut(s) 270, 373, 667, 694, 987
Alw21I GWGCWC 1 cut(s) 813
Alw26I GTCTC 2 cut(s) 125, 964
AlwI GGATC 2 cut(s) 811, 824
AoxI GGCC 4 cut(s) 39, 316, 501, 594
ApeKI GCWGC 5 cut(s) 296, 444, 511, 530, 851
ApoI RAATTY 4 cut(s) 290, 365, 433, 484
Asp718I GGTACC 1 cut(s) 197
AspLEI GCGC 3 cut(s) 139, 156, 705
AsuHPI GGTGA 1 cut(s) 914
BaeI ACNNNNGTAYC 2 cut(s) 189, 222
BalI TGGCCA 2 cut(s) 503, 596
BamHI GGATCC 1 cut(s) 816
BanI GGYRCC 2 cut(s) 197, 702
BarI GAAGNNNNNNTAC 2 cut(s) 783, 815
BbsI GAAGAC 1 cut(s) 791
Bbv12I GWGCWC 1 cut(s) 813
BbvI GCAGC 5 cut(s) 308, 431, 517, 523, 863
BccI CCATC 4 cut(s) 71, 493, 515, 623
BceAI ACGGC 2 cut(s) 150, 518
BciT130I CCWGG 2 cut(s) 614, 750
BciVI GTATCC 1 cut(s) 626
BcoDI GTCTC 2 cut(s) 125, 964
BfoI RGCGCY 1 cut(s) 706
BfuI GTATCC 1 cut(s) 626
BglII AGATCT 1 cut(s) 262
BisI GCNGC 5 cut(s) 297, 445, 512, 531, 852
BlsI GCNGC 5 cut(s) 298, 446, 513, 532, 853
Bme1390I CCNGG 2 cut(s) 614, 750
BmiI GGNNCC 4 cut(s) 109, 199, 704, 818
BmrFI CCNGG 2 cut(s) 614, 750
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 5 cut(s) 35, 181, 287, 523, 943
BmuI ACTGGG 1 cut(s) 228
BpiI GAAGAC 1 cut(s) 791
Bpu10I CCTNAGC 1 cut(s) 812
BpuEI CTTGAG 6 cut(s) 51, 97, 363, 692, 989, 1009
BsaHI GRCGYC 1 cut(s) 703
BsaJI CCNNGG 1 cut(s) 591
Bsc4I CCNNNNNNNGG 1 cut(s) 629
Bse1I ACTGG 2 cut(s) 88, 234
Bse3DI GCAATG 2 cut(s) 149, 924
BseBI CCWGG 2 cut(s) 614, 750
BseDI CCNNGG 1 cut(s) 591
BseGI GGATG 3 cut(s) 278, 504, 745
BseLI CCNNNNNNNGG 1 cut(s) 629
BseMI GCAATG 2 cut(s) 149, 924
BseMII CTCAG 3 cut(s) 280, 826, 956
BseNI ACTGG 2 cut(s) 88, 234
BseXI GCAGC 5 cut(s) 308, 431, 517, 523, 863
Bsh1236I CGCG 2 cut(s) 139, 156
BshFI GGCC 4 cut(s) 41, 318, 503, 596
BshNI GGYRCC 2 cut(s) 197, 702
BsiHKAI GWGCWC 1 cut(s) 813
BslFI GGGAC 1 cut(s) 605
BslI CCNNNNNNNGG 1 cut(s) 629
BsmAI GTCTC 2 cut(s) 125, 964
BsmBI CGTCTC 1 cut(s) 125
BsmFI GGGAC 1 cut(s) 605
BsnI GGCC 4 cut(s) 41, 318, 503, 596
Bsp1286I GDGCHC 1 cut(s) 813
Bsp143I GATC 2 cut(s) 262, 816
Bsp19I CCATGG 1 cut(s) 591
BspACI CCGC 3 cut(s) 156, 900, 914
BspANI GGCC 4 cut(s) 41, 318, 503, 596
BspCNI CTCAG 3 cut(s) 279, 825, 955
BspFNI CGCG 2 cut(s) 139, 156
BspLI GGNNCC 4 cut(s) 109, 199, 704, 818
BspPI GGATC 2 cut(s) 811, 824
BspT107I GGYRCC 2 cut(s) 197, 702
BsrDI GCAATG 2 cut(s) 149, 924
BsrI ACTGG 2 cut(s) 88, 234
BssECI CCNNGG 1 cut(s) 591
BssMI GATC 2 cut(s) 262, 816
BssNI GRCGYC 1 cut(s) 703
BssT1I CCWWGG 1 cut(s) 591
Bst2UI CCWGG 2 cut(s) 614, 750
Bst6I CTCTTC 2 cut(s) 49, 747
BstACI GRCGYC 1 cut(s) 703
BstAPI GCANNNNNTGC 1 cut(s) 953
BstC8I GCNNGC 3 cut(s) 442, 669, 684
BstDEI CTNAG 5 cut(s) 266, 551, 812, 942, 966
BstDSI CCRYGG 1 cut(s) 591
BstF5I GGATG 3 cut(s) 278, 504, 745
BstFNI CGCG 2 cut(s) 139, 156
BstH2I RGCGCY 1 cut(s) 706
BstHHI GCGC 3 cut(s) 139, 156, 705
BstKTI GATC 2 cut(s) 265, 819
BstMAI GTCTC 2 cut(s) 125, 964
BstMBI GATC 2 cut(s) 262, 816
BstMWI GCNNNNNNNGC 3 cut(s) 700, 953, 962
BstNI CCWGG 2 cut(s) 614, 750
BstNSI RCATGY 2 cut(s) 686, 864
BstSCI CCNGG 2 cut(s) 612, 748
BstUI CGCG 2 cut(s) 139, 156
BstV1I GCAGC 5 cut(s) 308, 431, 517, 523, 863
BstV2I GAAGAC 1 cut(s) 791
BstX2I RGATCY 2 cut(s) 262, 816
BstYI RGATCY 2 cut(s) 262, 816
BsuI GTATCC 1 cut(s) 626
BsuRI GGCC 4 cut(s) 41, 318, 503, 596
BtgI CCRYGG 1 cut(s) 591
BtsCI GGATG 3 cut(s) 278, 504, 745
BtsIMutI CAGTG 4 cut(s) 241, 597, 793, 872
Cac8I GCNNGC 3 cut(s) 442, 669, 684
CfoI GCGC 3 cut(s) 139, 156, 705
Csp6I GTAC 1 cut(s) 198
CviAII CATG 6 cut(s) 164, 253, 355, 592, 683, 861
CviQI GTAC 1 cut(s) 198
DdeI CTNAG 5 cut(s) 266, 551, 812, 942, 966
DinI GGCGCC 1 cut(s) 704
DpnI GATC 2 cut(s) 264, 818
DpnII GATC 2 cut(s) 262, 816
EaeI YGGCCR 2 cut(s) 501, 594
Eam1104I CTCTTC 2 cut(s) 49, 747
EarI CTCTTC 2 cut(s) 49, 747
Eco130I CCWWGG 1 cut(s) 591
Eco147I AGGCCT 1 cut(s) 318
Eco32I GATATC 2 cut(s) 784, 939
Eco57I CTGAAG 1 cut(s) 824
EcoRII CCWGG 2 cut(s) 612, 748
EcoRV GATATC 2 cut(s) 784, 939
EcoT14I CCWWGG 1 cut(s) 591
EcoT22I ATGCAT 1 cut(s) 777
EgeI GGCGCC 1 cut(s) 704
EheI GGCGCC 1 cut(s) 704
ErhI CCWWGG 1 cut(s) 591
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 6 cut(s) 167, 256, 358, 595, 686, 864
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 1 cut(s) 605
FatI CATG 6 cut(s) 163, 252, 354, 591, 682, 860
Fnu4HI GCNGC 5 cut(s) 297, 445, 512, 531, 852
FokI GGATG 3 cut(s) 265, 511, 732
Fsp4HI GCNGC 5 cut(s) 297, 445, 512, 531, 852
GlaI GCGC 3 cut(s) 138, 155, 704
GluI GCNGC 5 cut(s) 297, 445, 512, 531, 852
HaeII RGCGCY 1 cut(s) 706
HaeIII GGCC 4 cut(s) 41, 318, 503, 596
HhaI GCGC 3 cut(s) 139, 156, 705
Hin1I GRCGYC 1 cut(s) 703
Hin1II CATG 6 cut(s) 167, 256, 358, 595, 686, 864
Hin6I GCGC 3 cut(s) 137, 154, 703
HinP1I GCGC 3 cut(s) 137, 154, 703
HinfI GANTC 5 cut(s) 544, 587, 659, 877, 972
HphI GGTGA 1 cut(s) 914
Hpy188I TCNGA 1 cut(s) 804
Hpy188III TCNNGA 4 cut(s) 380, 518, 541, 814
HpyAV CCTTC 3 cut(s) 329, 438, 799
HpyCH4V TGCA 8 cut(s) 149, 296, 347, 404, 440, 682, 775, 956
HpyF10VI GCNNNNNNNGC 3 cut(s) 700, 953, 962
HpyF3I CTNAG 5 cut(s) 266, 551, 812, 942, 966
Hsp92I GRCGYC 1 cut(s) 703
Hsp92II CATG 6 cut(s) 167, 256, 358, 595, 686, 864
HspAI GCGC 3 cut(s) 137, 154, 703
KasI GGCGCC 1 cut(s) 702
KpnI GGTACC 1 cut(s) 201
Kzo9I GATC 2 cut(s) 262, 816
LmnI GCTCC 3 cut(s) 23, 107, 848
Lsp1109I GCAGC 5 cut(s) 308, 431, 517, 523, 863
LweI GCATC 5 cut(s) 35, 181, 287, 523, 943
MaeIII GTNAC 5 cut(s) 61, 100, 423, 468, 920
MalI GATC 2 cut(s) 264, 818
MboI GATC 2 cut(s) 262, 816
MboII GAAGA 4 cut(s) 36, 725, 734, 791
MfeI CAATTG 1 cut(s) 96
MflI RGATCY 2 cut(s) 262, 816
MhlI GDGCHC 1 cut(s) 813
MlsI TGGCCA 2 cut(s) 503, 596
MluCI AATT 9 cut(s) 96, 243, 290, 307, 365, 433, 484, 736, 756
MluNI TGGCCA 2 cut(s) 503, 596
Mly113I GGCGCC 1 cut(s) 703
MlyI GAGTC 3 cut(s) 538, 581, 966
MnlI CCTC 3 cut(s) 52, 121, 716
Mox20I TGGCCA 2 cut(s) 503, 596
Mph1103I ATGCAT 1 cut(s) 777
MscI TGGCCA 2 cut(s) 503, 596
MseI TTAA 7 cut(s) 207, 222, 339, 450, 474, 482, 888
MslI CAYNNNNRTG 2 cut(s) 519, 687
Msp20I TGGCCA 2 cut(s) 503, 596
MspR9I CCNGG 2 cut(s) 614, 750
MunI CAATTG 1 cut(s) 96
MvaI CCWGG 2 cut(s) 614, 750
MvnI CGCG 2 cut(s) 139, 156
MwoI GCNNNNNNNGC 3 cut(s) 700, 953, 962
NarI GGCGCC 1 cut(s) 703
NcoI CCATGG 1 cut(s) 591
NdeII GATC 2 cut(s) 262, 816
NlaIII CATG 6 cut(s) 167, 256, 358, 595, 686, 864
NlaIV GGNNCC 4 cut(s) 109, 199, 704, 818
NmeAIII GCCGAG 1 cut(s) 17
NsiI ATGCAT 1 cut(s) 777
NspI RCATGY 2 cut(s) 686, 864
PaeI GCATGC 1 cut(s) 686
PceI AGGCCT 1 cut(s) 318
PfeI GAWTC 2 cut(s) 659, 877
PfoI TCCNGGA 1 cut(s) 748
PkrI GCNGC 5 cut(s) 298, 446, 513, 532, 853
PleI GAGTC 3 cut(s) 538, 581, 966
PluTI GGCGCC 1 cut(s) 706
PpsI GAGTC 3 cut(s) 538, 581, 966
Psp6I CCWGG 2 cut(s) 612, 748
PspGI CCWGG 2 cut(s) 612, 748
PspN4I GGNNCC 4 cut(s) 109, 199, 704, 818
PsrI GAACNNNNNNTAC 2 cut(s) 73, 105
PsuI RGATCY 2 cut(s) 262, 816
RsaI GTAC 1 cut(s) 199
RsaNI GTAC 1 cut(s) 198
RseI CAYNNNNRTG 2 cut(s) 519, 687
SaqAI TTAA 7 cut(s) 207, 222, 339, 450, 474, 482, 888
SatI GCNGC 5 cut(s) 297, 445, 512, 531, 852
Sau3AI GATC 2 cut(s) 262, 816
SchI GAGTC 3 cut(s) 538, 581, 966
ScrFI CCNGG 2 cut(s) 614, 750
SduI GDGCHC 1 cut(s) 813
SetI ASST 9 cut(s) 272, 375, 421, 430, 618, 669, 696, 810, 989
SfaNI GCATC 5 cut(s) 35, 181, 287, 523, 943
SfoI GGCGCC 1 cut(s) 704
SmiMI CAYNNNNRTG 2 cut(s) 519, 687
SmlI CTYRAG 6 cut(s) 66, 112, 378, 707, 988, 1004
SmoI CTYRAG 6 cut(s) 66, 112, 378, 707, 988, 1004
SphI GCATGC 1 cut(s) 686
Sse9I AATT 9 cut(s) 96, 243, 290, 307, 365, 433, 484, 736, 756
SseBI AGGCCT 1 cut(s) 318
SsiI CCGC 3 cut(s) 156, 900, 914
SspDI GGCGCC 1 cut(s) 702
SspI AATATT 1 cut(s) 769
StuI AGGCCT 1 cut(s) 318
StyD4I CCNGG 2 cut(s) 612, 748
StyI CCWWGG 1 cut(s) 591
TasI AATT 9 cut(s) 96, 243, 290, 307, 365, 433, 484, 736, 756
TfiI GAWTC 2 cut(s) 659, 877
Tru1I TTAA 7 cut(s) 207, 222, 339, 450, 474, 482, 888
Tru9I TTAA 7 cut(s) 207, 222, 339, 450, 474, 482, 888
TscAI CASTG 4 cut(s) 241, 604, 793, 879
TseI GCWGC 5 cut(s) 296, 444, 511, 530, 851
TspDTI ATGAA 2 cut(s) 152, 734
TspGWI ACGGA 1 cut(s) 271
TspRI CASTG 4 cut(s) 241, 604, 793, 879
XapI RAATTY 4 cut(s) 290, 365, 433, 484
XceI RCATGY 2 cut(s) 686, 864
XcmI CCANNNNNNNNNTGG 1 cut(s) 865
Zsp2I ATGCAT 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.