Rmu_ssc0000467.1_g000046

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000467.1
N/A
Physical Location & Seq
Reverse (-)
164125 .. 168144
4020 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
atgcctgcaatgatggacgtccctacgagaggacgtagcgtcaccctacacggaggtaggcttgatgccaacgccaaagagagtgacactctgagagacaatggattcggacccatcacacaccaggcacgccgccaacactggcctgcgtttactatcctcatcccaaaacgacatgtgttttggaaggttttactgatgttgggtatttctttgacccacacaaaggtttcaaacgatgatcctggtcttcagttgtggaaagatgacaattgggttgcagtgaattacctaccaaatgcaatctttgttaacattggtgatcagatggaggtcttgagcaatggtaagtacaagagtattcttcacaggagcttggtgaacaagaagcagttacacatgtcgtgggctgtgttcattgtggcgccacatgaagctgtgattgggcctctgcgggagcttgtggacgagcaaaatcctgccaaatactcaaccaaaacatatggtgaataccgacaccacaaattcaataagatcccacagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01100 ko01110 map00941 map01100 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

181

Amino Acids

20.73

Weight (kDa)

9.69

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 78 - 140 1.1e-15 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 21
AccB1I GGYRCC 1 cut(s) 424
AciI CCGC 2 cut(s) 133, 454
AclWI GGATC 2 cut(s) 236, 529
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 1 cut(s) 236
AcyI GRCGYC 2 cut(s) 18, 425
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 2 cut(s) 29, 226
AflIII ACRYGT 2 cut(s) 175, 399
AgsI TTSAA 2 cut(s) 234, 529
AhdI GACNNNNNGTC 1 cut(s) 38
AjnI CCWGG 2 cut(s) 123, 244
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
AluBI AGCT 3 cut(s) 375, 437, 460
AluI AGCT 3 cut(s) 375, 437, 460
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 2 cut(s) 236, 529
AoxI GGCC 2 cut(s) 143, 446
ApoI RAATTY 1 cut(s) 524
ArsI GACNNNNNNTTYG 4 cut(s) 89, 121, 165, 197
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 2 cut(s) 110, 446
AsuHPI GGTGA 4 cut(s) 34, 332, 391, 518
AvaII GGWCC 1 cut(s) 110
BanI GGYRCC 1 cut(s) 424
BbsI GAAGAC 1 cut(s) 242
BccI CCATC 3 cut(s) 7, 122, 322
BciT130I CCWGG 2 cut(s) 125, 246
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 90
BfoI RGCGCY 1 cut(s) 428
BisI GCNGC 1 cut(s) 133
BlsI GCNGC 1 cut(s) 134
Bme1390I CCNGG 2 cut(s) 125, 246
Bme18I GGWCC 1 cut(s) 110
BmeRI GACNNNNNGTC 1 cut(s) 38
BmgT120I GGNCC 2 cut(s) 110, 446
BmiI GGNNCC 2 cut(s) 112, 426
BmrFI CCNGG 2 cut(s) 125, 246
BmsI GCATC 1 cut(s) 55
BpiI GAAGAC 1 cut(s) 242
BplI GAGNNNNNCTC 2 cut(s) 73, 105
BpuEI CTTGAG 1 cut(s) 358
BsaHI GRCGYC 2 cut(s) 18, 425
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 226
Bse1I ACTGG 1 cut(s) 146
Bse3DI GCAATG 2 cut(s) 15, 349
BseBI CCWGG 2 cut(s) 125, 246
BseGI GGATG 1 cut(s) 162
BseLI CCNNNNNNNGG 2 cut(s) 29, 226
BseMI GCAATG 2 cut(s) 15, 349
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 1 cut(s) 146
BshFI GGCC 2 cut(s) 145, 448
BshNI GGYRCC 1 cut(s) 424
BslFI GGGAC 1 cut(s) 5
BslI CCNNNNNNNGG 2 cut(s) 29, 226
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 5
BsnI GGCC 2 cut(s) 145, 448
Bsp143I GATC 3 cut(s) 241, 322, 534
BspACI CCGC 2 cut(s) 133, 454
BspANI GGCC 2 cut(s) 145, 448
BspCNI CTCAG 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 112, 426
BspPI GGATC 2 cut(s) 236, 529
BspT107I GGYRCC 1 cut(s) 424
BsrDI GCAATG 2 cut(s) 15, 349
BsrI ACTGG 1 cut(s) 146
BssMI GATC 3 cut(s) 241, 322, 534
BssNI GRCGYC 2 cut(s) 18, 425
Bst2UI CCWGG 2 cut(s) 125, 246
Bst4CI ACNGT 1 cut(s) 543
BstACI GRCGYC 2 cut(s) 18, 425
BstC8I GCNNGC 3 cut(s) 6, 130, 147
BstDEI CTNAG 1 cut(s) 92
BstENI CCTNNNNNAGG 1 cut(s) 27
BstF5I GGATG 1 cut(s) 162
BstH2I RGCGCY 1 cut(s) 428
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 3 cut(s) 244, 325, 537
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 3 cut(s) 241, 322, 534
BstNI CCWGG 2 cut(s) 125, 246
BstNSI RCATGY 2 cut(s) 179, 403
BstSCI CCNGG 2 cut(s) 123, 244
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 534
BstYI RGATCY 1 cut(s) 534
BsuRI GGCC 2 cut(s) 145, 448
BtsCI GGATG 1 cut(s) 162
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 2 cut(s) 139, 288
Cac8I GCNNGC 3 cut(s) 6, 130, 147
CfoI GCGC 1 cut(s) 427
Cfr13I GGNCC 2 cut(s) 110, 446
CseI GACGC 1 cut(s) 28
Csp6I GTAC 1 cut(s) 352
CviAII CATG 3 cut(s) 176, 400, 431
CviJI RGCY 7 cut(s) 61, 145, 375, 410, 437, 448, 460
CviKI_1 RGCY 7 cut(s) 61, 145, 375, 410, 437, 448, 460
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 1 cut(s) 92
DinI GGCGCC 1 cut(s) 426
DpnI GATC 3 cut(s) 243, 324, 536
DpnII GATC 3 cut(s) 241, 322, 534
DriI GACNNNNNGTC 1 cut(s) 38
Eam1105I GACNNNNNGTC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 110
Eco57I CTGAAG 1 cut(s) 236
EcoNI CCTNNNNNAGG 1 cut(s) 27
EcoRII CCWGG 2 cut(s) 123, 244
EgeI GGCGCC 1 cut(s) 426
EheI GGCGCC 1 cut(s) 426
FaeI CATG 3 cut(s) 179, 403, 434
FaiI YATR 5 cut(s) 177, 401, 432, 502, 504
FaqI GGGAC 1 cut(s) 5
FatI CATG 3 cut(s) 175, 399, 430
FauI CCCGC 1 cut(s) 447
FauNDI CATATG 1 cut(s) 502
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 133
FokI GGATG 1 cut(s) 149
Fsp4HI GCNGC 1 cut(s) 133
GlaI GCGC 1 cut(s) 426
GluI GCNGC 1 cut(s) 133
HaeII RGCGCY 1 cut(s) 428
HaeIII GGCC 2 cut(s) 145, 448
HgaI GACGC 1 cut(s) 28
HhaI GCGC 1 cut(s) 427
Hin1I GRCGYC 2 cut(s) 18, 425
Hin1II CATG 3 cut(s) 179, 403, 434
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 1 cut(s) 105
HpaI GTTAAC 1 cut(s) 313
HphI GGTGA 4 cut(s) 34, 332, 391, 518
Hpy166II GTNNAC 4 cut(s) 153, 313, 382, 466
Hpy188I TCNGA 3 cut(s) 93, 110, 327
Hpy188III TCNNGA 1 cut(s) 337
Hpy8I GTNNAC 4 cut(s) 153, 313, 382, 466
HpyAV CCTTC 1 cut(s) 181
HpyCH4III ACNGT 1 cut(s) 543
HpyCH4IV ACGT 2 cut(s) 18, 34
HpyCH4V TGCA 3 cut(s) 8, 281, 302
HpyF3I CTNAG 1 cut(s) 92
HpySE526I ACGT 2 cut(s) 18, 34
Hsp92I GRCGYC 2 cut(s) 18, 425
Hsp92II CATG 3 cut(s) 179, 403, 434
HspAI GCGC 1 cut(s) 425
KasI GGCGCC 1 cut(s) 424
Ksp22I TGATCA 1 cut(s) 322
KspAI GTTAAC 1 cut(s) 313
Kzo9I GATC 3 cut(s) 241, 322, 534
LmnI GCTCC 2 cut(s) 372, 457
LpnPI CCDG 9 cut(s) 18, 110, 127, 137, 159, 231, 258, 355, 492
LweI GCATC 1 cut(s) 55
MaeII ACGT 2 cut(s) 18, 34
MaeIII GTNAC 3 cut(s) 40, 83, 393
MalI GATC 3 cut(s) 243, 324, 536
MboI GATC 3 cut(s) 241, 322, 534
MboII GAAGA 2 cut(s) 242, 356
MfeI CAATTG 1 cut(s) 271
MflI RGATCY 1 cut(s) 534
MluCI AATT 3 cut(s) 271, 286, 524
Mly113I GGCGCC 1 cut(s) 425
MnlI CCTC 5 cut(s) 23, 47, 170, 325, 459
MseI TTAA 1 cut(s) 312
MspR9I CCNGG 2 cut(s) 125, 246
MunI CAATTG 1 cut(s) 271
MvaI CCWGG 2 cut(s) 125, 246
NarI GGCGCC 1 cut(s) 425
NdeI CATATG 1 cut(s) 502
NdeII GATC 3 cut(s) 241, 322, 534
NlaIII CATG 3 cut(s) 179, 403, 434
NlaIV GGNNCC 2 cut(s) 112, 426
NmuCI GTSAC 2 cut(s) 40, 83
NspI RCATGY 2 cut(s) 179, 403
PciI ACATGT 2 cut(s) 175, 399
PfeI GAWTC 1 cut(s) 105
PkrI GCNGC 1 cut(s) 134
PluTI GGCGCC 1 cut(s) 428
PscI ACATGT 2 cut(s) 175, 399
Psp6I CCWGG 2 cut(s) 123, 244
PspGI CCWGG 2 cut(s) 123, 244
PspN4I GGNNCC 2 cut(s) 112, 426
PspPI GGNCC 2 cut(s) 110, 446
PsuI RGATCY 1 cut(s) 534
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 1 cut(s) 133
Sau3AI GATC 3 cut(s) 241, 322, 534
Sau96I GGNCC 2 cut(s) 110, 446
ScrFI CCNGG 2 cut(s) 125, 246
SfaNI GCATC 1 cut(s) 55
SfoI GGCGCC 1 cut(s) 426
SinI GGWCC 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 337
SmoI CTYRAG 1 cut(s) 337
Sse9I AATT 3 cut(s) 271, 286, 524
SsiI CCGC 2 cut(s) 133, 454
SspDI GGCGCC 1 cut(s) 424
StyD4I CCNGG 2 cut(s) 123, 244
TaaI ACNGT 1 cut(s) 543
TaiI ACGT 2 cut(s) 21, 37
TasI AATT 3 cut(s) 271, 286, 524
TatI WGTACW 1 cut(s) 351
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 1 cut(s) 105
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TscAI CASTG 2 cut(s) 146, 288
TseFI GTSAC 2 cut(s) 40, 83
Tsp45I GTSAC 2 cut(s) 40, 83
TspDTI ATGAA 2 cut(s) 406, 447
TspGWI ACGGA 1 cut(s) 66
TspRI CASTG 2 cut(s) 146, 288
VpaK11BI GGWCC 1 cut(s) 110
XagI CCTNNNNNAGG 1 cut(s) 27
XapI RAATTY 1 cut(s) 524
XceI RCATGY 2 cut(s) 179, 403
ZraI GACGTC 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.