Rmu_ssc0000474.1_g000001

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000474.1
N/A
Physical Location & Seq
Reverse (-)
2569 .. 4573
2005 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 552 bp
atgaatcgaagaatcaagatcctccatccggagagtttccaaatcggagctgatgtcaccaccattcttcacatgtttcctttgcatcgtcgtggaatcaaggcctccaccactttcaggagaaagggcggagaacacgaaagccgagcaatcttcaccgctttctttaaggaggaatcaagggccctcgcatctctctttgctgagatggttgttcctccaccagtcaggccacctactattacaaagtttctaaagccttatgtcctgaaaatgcactttacaaataaatatgtaagtgcccaggtagtccactcaccaactgccactgtagcctcttctgcaagctcgcaggaaaaagccttgagagagagcctggagtcgcatagggatgttgctgctgctgcaaagattgggaagatattaggggagcgcctgctgctcaagaatatccctgctgtatcagtccacttgaagagagaacagaaatatcatggtaaggttaaagctgtcattgataatgtggtagaagctggtgtcaaactactctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

183

Amino Acids

20.41

Weight (kDa)

10.34

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 89 - 182 1.1e-12 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 28
AciI CCGC 2 cut(s) 129, 159
AclWI GGATC 1 cut(s) 13
AfiI CCNNNNNNNGG 2 cut(s) 28, 117
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 1 cut(s) 475
AjnI CCWGG 2 cut(s) 303, 375
AluBI AGCT 4 cut(s) 50, 348, 509, 533
AluI AGCT 4 cut(s) 50, 348, 509, 533
AlwI GGATC 1 cut(s) 13
Aor13HI TCCGGA 1 cut(s) 28
AoxI GGCC 3 cut(s) 102, 183, 230
ApaI GGGCCC 1 cut(s) 187
ApeKI GCWGC 4 cut(s) 398, 401, 404, 439
AspLEI GCGC 1 cut(s) 435
AspS9I GGNCC 2 cut(s) 183, 184
AsuHPI GGTGA 3 cut(s) 49, 148, 309
BaeGI GKGCMC 2 cut(s) 187, 304
BanII GRGCYC 1 cut(s) 187
BbvI GCAGC 4 cut(s) 385, 388, 391, 426
BccI CCATC 2 cut(s) 33, 202
BciT130I CCWGG 2 cut(s) 305, 377
BfmI CTRYAG 1 cut(s) 330
BfoI RGCGCY 1 cut(s) 436
BisI GCNGC 4 cut(s) 399, 402, 405, 440
BlsI GCNGC 4 cut(s) 400, 403, 406, 441
Bme1390I CCNGG 2 cut(s) 305, 377
BmgT120I GGNCC 2 cut(s) 183, 184
BmiI GGNNCC 1 cut(s) 185
BmrFI CCNGG 2 cut(s) 305, 377
BmsI GCATC 2 cut(s) 94, 200
BpmI CTGGAG 1 cut(s) 398
BpuEI CTTGAG 2 cut(s) 385, 428
BsaJI CCNNGG 1 cut(s) 303
BsaWI WCCGGW 1 cut(s) 28
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 117
Bse1I ACTGG 1 cut(s) 224
BseAI TCCGGA 1 cut(s) 28
BseBI CCWGG 2 cut(s) 305, 377
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 2 cut(s) 25, 397
BseLI CCNNNNNNNGG 2 cut(s) 28, 117
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 1 cut(s) 224
BseSI GKGCMC 2 cut(s) 187, 304
BseXI GCAGC 4 cut(s) 385, 388, 391, 426
BshFI GGCC 3 cut(s) 104, 185, 232
BsiSI CCGG 1 cut(s) 29
BslI CCNNNNNNNGG 2 cut(s) 28, 117
BsnI GGCC 3 cut(s) 104, 185, 232
Bsp120I GGGCCC 1 cut(s) 183
Bsp1286I GDGCHC 2 cut(s) 187, 304
Bsp13I TCCGGA 1 cut(s) 28
Bsp143I GATC 1 cut(s) 18
BspACI CCGC 2 cut(s) 129, 159
BspANI GGCC 3 cut(s) 104, 185, 232
BspCNI CTCAG 1 cut(s) 196
BspEI TCCGGA 1 cut(s) 28
BspLI GGNNCC 1 cut(s) 185
BspPI GGATC 1 cut(s) 13
BsrI ACTGG 1 cut(s) 224
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 305, 377
Bst4CI ACNGT 1 cut(s) 331
Bst6I CTCTTC 2 cut(s) 343, 470
BstC8I GCNNGC 3 cut(s) 346, 350, 437
BstDEI CTNAG 1 cut(s) 204
BstF5I GGATG 2 cut(s) 25, 397
BstH2I RGCGCY 1 cut(s) 436
BstHHI GCGC 1 cut(s) 435
BstKTI GATC 1 cut(s) 21
BstMBI GATC 1 cut(s) 18
BstMWI GCNNNNNNNGC 4 cut(s) 332, 341, 404, 439
BstNI CCWGG 2 cut(s) 305, 377
BstNSI RCATGY 1 cut(s) 76
BstSCI CCNGG 2 cut(s) 303, 375
BstSFI CTRYAG 1 cut(s) 330
BstSLI GKGCMC 2 cut(s) 187, 304
BstV1I GCAGC 4 cut(s) 385, 388, 391, 426
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
BsuRI GGCC 3 cut(s) 104, 185, 232
BtsCI GGATG 2 cut(s) 25, 397
BtsIMutI CAGTG 1 cut(s) 327
Cac8I GCNNGC 3 cut(s) 346, 350, 437
CfoI GCGC 1 cut(s) 435
Cfr13I GGNCC 2 cut(s) 183, 184
CviAII CATG 2 cut(s) 73, 494
DdeI CTNAG 1 cut(s) 204
DpnI GATC 1 cut(s) 20
DpnII GATC 1 cut(s) 18
Eam1104I CTCTTC 2 cut(s) 343, 470
EarI CTCTTC 2 cut(s) 343, 470
EciI GGCGGA 1 cut(s) 144
Eco147I AGGCCT 1 cut(s) 104
Eco24I GRGCYC 1 cut(s) 187
EcoO109I RGGNCCY 2 cut(s) 183, 184
EcoRII CCWGG 2 cut(s) 303, 375
EcoT38I GRGCYC 1 cut(s) 187
FaeI CATG 2 cut(s) 76, 497
FaiI YATR 5 cut(s) 74, 264, 294, 387, 495
FatI CATG 2 cut(s) 72, 493
Fnu4HI GCNGC 4 cut(s) 399, 402, 405, 440
FokI GGATG 2 cut(s) 12, 404
FriOI GRGCYC 1 cut(s) 187
Fsp4HI GCNGC 4 cut(s) 399, 402, 405, 440
GlaI GCGC 1 cut(s) 434
GluI GCNGC 4 cut(s) 399, 402, 405, 440
GsuI CTGGAG 1 cut(s) 398
HaeII RGCGCY 1 cut(s) 436
HaeIII GGCC 3 cut(s) 104, 185, 232
HapII CCGG 1 cut(s) 29
HhaI GCGC 1 cut(s) 435
Hin1II CATG 2 cut(s) 76, 497
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HinfI GANTC 5 cut(s) 4, 12, 96, 176, 380
HpaII CCGG 1 cut(s) 29
HphI GGTGA 3 cut(s) 49, 148, 309
Hpy166II GTNNAC 2 cut(s) 313, 469
Hpy188I TCNGA 2 cut(s) 47, 551
Hpy188III TCNNGA 5 cut(s) 16, 29, 118, 268, 445
Hpy8I GTNNAC 2 cut(s) 313, 469
Hpy99I CGWCG 1 cut(s) 93
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4V TGCA 4 cut(s) 85, 277, 344, 407
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 341, 404, 439
HpyF3I CTNAG 1 cut(s) 204
Hsp92II CATG 2 cut(s) 76, 497
HspAI GCGC 1 cut(s) 433
Kpn2I TCCGGA 1 cut(s) 28
Kzo9I GATC 1 cut(s) 18
LmnI GCTCC 2 cut(s) 47, 430
Lsp1109I GCAGC 4 cut(s) 385, 388, 391, 426
LweI GCATC 2 cut(s) 94, 200
MaeIII GTNAC 1 cut(s) 55
MalI GATC 1 cut(s) 20
MboI GATC 1 cut(s) 18
MboII GAAGA 6 cut(s) 21, 59, 145, 330, 430, 487
MflI RGATCY 1 cut(s) 18
MhlI GDGCHC 2 cut(s) 187, 304
MlyI GAGTC 1 cut(s) 389
MnlI CCTC 6 cut(s) 32, 115, 166, 197, 228, 346
MroI TCCGGA 1 cut(s) 28
MseI TTAA 2 cut(s) 168, 504
MslI CAYNNNNRTG 2 cut(s) 90, 390
MspI CCGG 1 cut(s) 29
MspR9I CCNGG 2 cut(s) 305, 377
MvaI CCWGG 2 cut(s) 305, 377
MwoI GCNNNNNNNGC 4 cut(s) 332, 341, 404, 439
NdeII GATC 1 cut(s) 18
NlaIII CATG 2 cut(s) 76, 497
NlaIV GGNNCC 1 cut(s) 185
NmeAIII GCCGAG 1 cut(s) 170
NmuCI GTSAC 1 cut(s) 55
NspI RCATGY 1 cut(s) 76
PceI AGGCCT 1 cut(s) 104
PciI ACATGT 1 cut(s) 72
PfeI GAWTC 4 cut(s) 4, 12, 96, 176
PkrI GCNGC 4 cut(s) 400, 403, 406, 441
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
PscI ACATGT 1 cut(s) 72
Psp6I CCWGG 2 cut(s) 303, 375
PspGI CCWGG 2 cut(s) 303, 375
PspN4I GGNNCC 1 cut(s) 185
PspOMI GGGCCC 1 cut(s) 183
PspPI GGNCC 2 cut(s) 183, 184
PsuI RGATCY 1 cut(s) 18
RseI CAYNNNNRTG 2 cut(s) 90, 390
SaqAI TTAA 2 cut(s) 168, 504
SatI GCNGC 4 cut(s) 399, 402, 405, 440
Sau3AI GATC 1 cut(s) 18
Sau96I GGNCC 2 cut(s) 183, 184
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 305, 377
SduI GDGCHC 2 cut(s) 187, 304
SetI ASST 7 cut(s) 52, 238, 309, 350, 504, 511, 535
SfaNI GCATC 2 cut(s) 94, 200
SfcI CTRYAG 1 cut(s) 330
SmiMI CAYNNNNRTG 2 cut(s) 90, 390
SmlI CTYRAG 2 cut(s) 364, 443
SmoI CTYRAG 2 cut(s) 364, 443
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 2 cut(s) 129, 159
StuI AGGCCT 1 cut(s) 104
StyD4I CCNGG 2 cut(s) 303, 375
TaaI ACNGT 1 cut(s) 331
TaqI TCGA 1 cut(s) 7
TfiI GAWTC 4 cut(s) 4, 12, 96, 176
Tru1I TTAA 2 cut(s) 168, 504
Tru9I TTAA 2 cut(s) 168, 504
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 4 cut(s) 398, 401, 404, 439
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 334
XceI RCATGY 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.