Rmu_ssc0000480.1_g000028

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000480.1
OG0031025
Physical Location & Seq
Forward (+)
142759 .. 143088
330 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 330 bp
atgtcacccacccttacagaccaatccaaatcccaaatcctctccacccgcgaatccctcctctcccgccaaatcaccgccgtcgagcttgctgagtccttccttgccctcctctgccgcaccgagccccacctccgctccatcttccacgtctccgacgccgtcctcgcccaggcccgcgaccttgacgacaagcttcgcctgaatgaggaggtgggacccttggcctgggtccttgtggcggtgaaggataacatatgtacggcggagatgccgtccacggcagggtccagggtccaaggttttggagggctatacgccgccgtatga

Protein Analysis

109

Amino Acids

11.84

Weight (kDa)

5.25

Isoelectric Point (pI)

61.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 29 - 99 5.3e-10 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0031025)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8457111.1_g000001 Rmu_ssc0000480.1_g000028

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 138
AccII CGCG 2 cut(s) 51, 180
AciI CCGC 9 cut(s) 49, 67, 78, 118, 136, 178, 242, 266, 321
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 1 cut(s) 262
AfiI CCNNNNNNNGG 5 cut(s) 172, 208, 228, 241, 285
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 3 cut(s) 171, 227, 290
AluBI AGCT 2 cut(s) 88, 196
AluI AGCT 2 cut(s) 88, 196
Alw26I GTCTC 1 cut(s) 157
AoxI GGCC 2 cut(s) 174, 225
AspS9I GGNCC 5 cut(s) 175, 218, 232, 288, 295
AsuHPI GGTGA 2 cut(s) 67, 256
AvaII GGWCC 4 cut(s) 218, 232, 288, 295
BanII GRGCYC 1 cut(s) 129
BccI CCATC 1 cut(s) 149
BceAI ACGGC 6 cut(s) 65, 146, 259, 279, 297, 308
BciT130I CCWGG 3 cut(s) 173, 229, 292
BcoDI GTCTC 1 cut(s) 157
BisI GCNGC 2 cut(s) 118, 321
BlsI GCNGC 2 cut(s) 119, 322
Bme1390I CCNGG 3 cut(s) 173, 229, 292
Bme18I GGWCC 4 cut(s) 218, 232, 288, 295
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 5 cut(s) 175, 218, 232, 288, 295
BmiI GGNNCC 5 cut(s) 219, 220, 233, 289, 296
BmrFI CCNGG 3 cut(s) 173, 229, 292
BmsI GCATC 1 cut(s) 261
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 6 cut(s) 171, 222, 228, 279, 291, 298
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 260, 290
Bsc4I CCNNNNNNNGG 5 cut(s) 172, 208, 228, 241, 285
BseBI CCWGG 3 cut(s) 173, 229, 292
BseDI CCNNGG 6 cut(s) 171, 222, 228, 279, 291, 298
BseLI CCNNNNNNNGG 5 cut(s) 172, 208, 228, 241, 285
BseMII CTCAG 1 cut(s) 84
BseRI GAGGAG 3 cut(s) 50, 101, 224
Bsh1236I CGCG 2 cut(s) 51, 180
BshFI GGCC 2 cut(s) 176, 227
BslFI GGGAC 1 cut(s) 231
BslI CCNNNNNNNGG 5 cut(s) 172, 208, 228, 241, 285
BsmAI GTCTC 1 cut(s) 157
BsmBI CGTCTC 1 cut(s) 157
BsmFI GGGAC 1 cut(s) 231
BsnI GGCC 2 cut(s) 176, 227
Bsp1286I GDGCHC 1 cut(s) 129
BspACI CCGC 9 cut(s) 49, 67, 78, 118, 136, 178, 242, 266, 321
BspANI GGCC 2 cut(s) 176, 227
BspCNI CTCAG 1 cut(s) 85
BspFNI CGCG 2 cut(s) 51, 180
BspLI GGNNCC 5 cut(s) 219, 220, 233, 289, 296
BsrBI CCGCTC 1 cut(s) 138
BssECI CCNNGG 6 cut(s) 171, 222, 228, 279, 291, 298
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 2 cut(s) 222, 298
Bst2UI CCWGG 3 cut(s) 173, 229, 292
BstACI GRCGYC 1 cut(s) 159
BstC8I GCNNGC 2 cut(s) 90, 178
BstDEI CTNAG 1 cut(s) 93
BstDSI CCRYGG 1 cut(s) 279
BstENI CCTNNNNNAGG 2 cut(s) 170, 206
BstFNI CGCG 2 cut(s) 51, 180
BstMAI GTCTC 1 cut(s) 157
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstNI CCWGG 3 cut(s) 173, 229, 292
BstSCI CCNGG 3 cut(s) 171, 227, 290
BstUI CGCG 2 cut(s) 51, 180
BstXI CCANNNNNNTGG 1 cut(s) 305
BsuRI GGCC 2 cut(s) 176, 227
BtgI CCRYGG 1 cut(s) 279
BtrI CACGTC 1 cut(s) 151
Cac8I GCNNGC 2 cut(s) 90, 178
Cfr13I GGNCC 5 cut(s) 175, 218, 232, 288, 295
CseI GACGC 1 cut(s) 167
Csp6I GTAC 1 cut(s) 261
CviJI RGCY 6 cut(s) 88, 127, 176, 196, 227, 313
CviKI_1 RGCY 6 cut(s) 88, 127, 176, 196, 227, 313
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 1 cut(s) 93
EciI GGCGGA 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 222, 298
Eco24I GRGCYC 1 cut(s) 129
Eco47I GGWCC 4 cut(s) 218, 232, 288, 295
EcoNI CCTNNNNNAGG 2 cut(s) 170, 206
EcoO109I RGGNCCY 2 cut(s) 218, 232
EcoRII CCWGG 3 cut(s) 171, 227, 290
EcoT14I CCWWGG 2 cut(s) 222, 298
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 2 cut(s) 222, 298
Esp3I CGTCTC 1 cut(s) 157
FaiI YATR 4 cut(s) 257, 259, 316, 328
FaqI GGGAC 1 cut(s) 231
FauI CCCGC 3 cut(s) 56, 74, 185
FauNDI CATATG 1 cut(s) 257
Fnu4HI GCNGC 2 cut(s) 118, 321
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 2 cut(s) 118, 321
GluI GCNGC 2 cut(s) 118, 321
HaeIII GGCC 2 cut(s) 176, 227
HgaI GACGC 1 cut(s) 167
Hin1I GRCGYC 1 cut(s) 159
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 2 cut(s) 53, 95
HphI GGTGA 2 cut(s) 67, 256
Hpy166II GTNNAC 1 cut(s) 279
Hpy188I TCNGA 1 cut(s) 157
Hpy8I GTNNAC 1 cut(s) 279
Hpy99I CGWCG 2 cut(s) 86, 161
HpyAV CCTTC 2 cut(s) 109, 241
HpyCH4IV ACGT 1 cut(s) 150
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 1 cut(s) 93
HpySE526I ACGT 1 cut(s) 150
Hsp92I GRCGYC 1 cut(s) 159
KflI GGGWCCC 1 cut(s) 218
LmnI GCTCC 1 cut(s) 143
LpnPI CCDG 8 cut(s) 158, 185, 214, 215, 241, 270, 277, 304
LweI GCATC 1 cut(s) 261
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 3
MbiI CCGCTC 1 cut(s) 138
MboII GAAGA 1 cut(s) 136
MhlI GDGCHC 1 cut(s) 129
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 180
MspR9I CCNGG 3 cut(s) 173, 229, 292
MvaI CCWGG 3 cut(s) 173, 229, 292
MvnI CGCG 2 cut(s) 51, 180
MwoI GCNNNNNNNGC 1 cut(s) 167
NdeI CATATG 1 cut(s) 257
NlaIV GGNNCC 5 cut(s) 219, 220, 233, 289, 296
NmuCI GTSAC 1 cut(s) 3
PfeI GAWTC 1 cut(s) 53
PflFI GACNNNGTC 1 cut(s) 161
PkrI GCNGC 2 cut(s) 119, 322
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
PpuMI RGGWCCY 2 cut(s) 218, 232
Psp5II RGGWCCY 2 cut(s) 218, 232
Psp6I CCWGG 3 cut(s) 171, 227, 290
PspGI CCWGG 3 cut(s) 171, 227, 290
PspN4I GGNNCC 5 cut(s) 219, 220, 233, 289, 296
PspPI GGNCC 5 cut(s) 175, 218, 232, 288, 295
PspPPI RGGWCCY 2 cut(s) 218, 232
PsyI GACNNNGTC 1 cut(s) 161
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
SatI GCNGC 2 cut(s) 118, 321
Sau96I GGNCC 5 cut(s) 175, 218, 232, 288, 295
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 3 cut(s) 173, 229, 292
SduI GDGCHC 1 cut(s) 129
SetI ASST 7 cut(s) 90, 135, 153, 186, 198, 216, 304
SfaNI GCATC 1 cut(s) 261
SinI GGWCC 4 cut(s) 218, 232, 288, 295
SsiI CCGC 9 cut(s) 49, 67, 78, 118, 136, 178, 242, 266, 321
StyD4I CCNGG 3 cut(s) 171, 227, 290
StyI CCWWGG 2 cut(s) 222, 298
TaiI ACGT 1 cut(s) 153
TaqI TCGA 1 cut(s) 84
TauI GCSGC 2 cut(s) 120, 323
TfiI GAWTC 1 cut(s) 53
TseFI GTSAC 1 cut(s) 3
Tsp45I GTSAC 1 cut(s) 3
Tth111I GACNNNGTC 1 cut(s) 161
VpaK11BI GGWCC 4 cut(s) 218, 232, 288, 295
XagI CCTNNNNNAGG 2 cut(s) 170, 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.