Rroxscaffold_164G00436410

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000164
N/A
Physical Location & Seq
Forward (+)
313965 .. 320930
6966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_164G00436410.1

Sequence Viewer

Length: 696 bp
ATGGGATCGCTAAACACGGCGATCCGGATCGCAAATGCCCCGCGATTGGGTTCGTTCGATCCGGAACGCGAGTCGCCGGGGAGGCGGCGTTTCGGTGACTTTGGATATGACACCGTCTTCGGTGTCGAGCGACCATTTGCCACCGAAGTTGGAAGACATGGGAGCTCGGCAAAGATTGAAGGCGCTTGCGATTACTTGTATGTTCAAAACATAACGATAATATATGCACTAGTATATGCGCATTGCAGCCATATTGCTAACTCTCGTGGCCATAACGAATTGCAGGTTAAACTATTGATGACAGCTCATCATCGAAACTTGGTTTCTCTCATTGGTTACTGCGATGAAGGTGACACTATGGCACTTGTTTTTGAGTATGTAGCCAATGGAAATTTGCAGCAACATTTATCAGCCAAAGCACTTTTGCCTTGCAACTTACCAAACACCTATAAATTGCTTTTGGAGCTCACAGCACTTTTAAAAACAGTTTACCAAACACCTCAGCTGCTTCTCCTCACAGCAAGTTCGGAAGTGCTTCCTCTCATAGCAAGTTCGGAAGTGCTTCCTCTCACAGCACAGCAATCCCAAACTAAGCCTAAGTCTTCCCGGCCAAACTCATACAGAGGTGATAGTGACGCTTCAATGCGTCCCGGAATGTATGAAACATCACATGCTTACGAATTACCTATTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

231

Amino Acids

25.49

Weight (kDa)

6.44

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 95 - 161 1.3e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 240
Acc36I ACCTGC 1 cut(s) 274
AccII CGCG 2 cut(s) 43, 69
AccIII TCCGGA 2 cut(s) 24, 61
AciI CCGC 2 cut(s) 41, 85
AclWI GGATC 4 cut(s) 13, 16, 35, 53
AcoI YGGCCR 2 cut(s) 268, 608
AcsI RAATTY 1 cut(s) 391
AfiI CCNNNNNNNGG 2 cut(s) 46, 47
AgsI TTSAA 3 cut(s) 179, 206, 642
AhlI ACTAGT 1 cut(s) 229
AluBI AGCT 4 cut(s) 165, 305, 466, 505
AluI AGCT 4 cut(s) 165, 305, 466, 505
Alw21I GWGCWC 2 cut(s) 167, 468
AlwI GGATC 4 cut(s) 13, 16, 35, 53
Aor13HI TCCGGA 2 cut(s) 24, 61
AoxI GGCC 2 cut(s) 268, 608
ApeKI GCWGC 3 cut(s) 246, 397, 505
ApoI RAATTY 1 cut(s) 391
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
Asp700I GAANNNNTTC 2 cut(s) 534, 561
AspLEI GCGC 2 cut(s) 185, 241
AsuC2I CCSGG 3 cut(s) 78, 607, 651
AsuHPI GGTGA 3 cut(s) 107, 362, 638
BalI TGGCCA 1 cut(s) 270
BanII GRGCYC 2 cut(s) 167, 468
BauI CACGAG 1 cut(s) 264
BbsI GAAGAC 3 cut(s) 109, 160, 594
Bbv12I GWGCWC 2 cut(s) 167, 468
BbvCI CCTCAGC 1 cut(s) 501
BbvI GCAGC 3 cut(s) 258, 409, 492
BceAI ACGGC 1 cut(s) 33
BcnI CCSGG 3 cut(s) 78, 607, 651
BcuI ACTAGT 1 cut(s) 229
BfaI CTAG 1 cut(s) 230
BfoI RGCGCY 1 cut(s) 186
BfuAI ACCTGC 1 cut(s) 274
BglI GCCNNNNNGGC 1 cut(s) 82
BisI GCNGC 4 cut(s) 86, 247, 398, 506
BlsI GCNGC 4 cut(s) 87, 248, 399, 507
Bme1390I CCNGG 3 cut(s) 78, 607, 651
BmrFI CCNGG 3 cut(s) 78, 607, 651
BpiI GAAGAC 3 cut(s) 109, 160, 594
Bpu10I CCTNAGC 1 cut(s) 501
BpuMI CCSGG 3 cut(s) 78, 607, 651
BsaBI GATNNNNATC 1 cut(s) 26
BsaJI CCNNGG 1 cut(s) 77
BsaWI WCCGGW 2 cut(s) 24, 61
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 47
Bse3DI GCAATG 1 cut(s) 241
Bse8I GATNNNNATC 1 cut(s) 26
BseAI TCCGGA 2 cut(s) 24, 61
BseDI CCNNGG 1 cut(s) 77
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 2 cut(s) 46, 47
BseMI GCAATG 1 cut(s) 241
BseMII CTCAG 1 cut(s) 515
BseRI GAGGAG 1 cut(s) 503
BseXI GCAGC 3 cut(s) 258, 409, 492
Bsh1236I CGCG 2 cut(s) 43, 69
BshFI GGCC 2 cut(s) 270, 610
BsiHKAI GWGCWC 2 cut(s) 167, 468
BsiSI CCGG 5 cut(s) 25, 62, 77, 607, 651
BslFI GGGAC 1 cut(s) 633
BslI CCNNNNNNNGG 2 cut(s) 46, 47
BsmFI GGGAC 1 cut(s) 633
BsnI GGCC 2 cut(s) 270, 610
Bsp1286I GDGCHC 2 cut(s) 167, 468
Bsp13I TCCGGA 2 cut(s) 24, 61
Bsp143I GATC 4 cut(s) 5, 21, 27, 58
BspACI CCGC 2 cut(s) 41, 85
BspANI GGCC 2 cut(s) 270, 610
BspCNI CTCAG 1 cut(s) 514
BspEI TCCGGA 2 cut(s) 24, 61
BspFNI CGCG 2 cut(s) 43, 69
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 4 cut(s) 13, 16, 35, 53
BsrDI GCAATG 1 cut(s) 241
BssECI CCNNGG 1 cut(s) 77
BssMI GATC 4 cut(s) 5, 21, 27, 58
BssSI CACGAG 1 cut(s) 264
Bst2BI CACGAG 1 cut(s) 264
Bst4CI ACNGT 2 cut(s) 115, 487
BstC8I GCNNGC 1 cut(s) 187
BstDEI CTNAG 3 cut(s) 501, 591, 597
BstFNI CGCG 2 cut(s) 43, 69
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 2 cut(s) 185, 241
BstKTI GATC 4 cut(s) 8, 24, 30, 61
BstMBI GATC 4 cut(s) 5, 21, 27, 58
BstMWI GCNNNNNNNGC 2 cut(s) 82, 463
BstNSI RCATGY 1 cut(s) 674
BstSCI CCNGG 3 cut(s) 76, 605, 649
BstUI CGCG 2 cut(s) 43, 69
BstV1I GCAGC 3 cut(s) 258, 409, 492
BstV2I GAAGAC 3 cut(s) 109, 160, 594
BsuRI GGCC 2 cut(s) 270, 610
BtgZI GCGATG 1 cut(s) 357
BveI ACCTGC 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 187
CfoI GCGC 2 cut(s) 185, 241
CseI GACGC 2 cut(s) 635, 644
CspCI CAANNNNNGTGG 2 cut(s) 130, 165
CviAII CATG 2 cut(s) 158, 671
DdeI CTNAG 3 cut(s) 501, 591, 597
DpnI GATC 4 cut(s) 7, 23, 29, 60
DpnII GATC 4 cut(s) 5, 21, 27, 58
DraI TTTAAA 1 cut(s) 480
EaeI YGGCCR 2 cut(s) 268, 608
Ecl136II GAGCTC 2 cut(s) 165, 466
Eco24I GRGCYC 2 cut(s) 167, 468
Eco53kI GAGCTC 2 cut(s) 165, 466
EcoICRI GAGCTC 2 cut(s) 165, 466
EcoT38I GRGCYC 2 cut(s) 167, 468
FaeI CATG 2 cut(s) 161, 674
FaqI GGGAC 1 cut(s) 633
FatI CATG 2 cut(s) 157, 670
FauI CCCGC 1 cut(s) 48
Fnu4HI GCNGC 4 cut(s) 86, 247, 398, 506
FriOI GRGCYC 2 cut(s) 167, 468
Fsp4HI GCNGC 4 cut(s) 86, 247, 398, 506
FspAI RTGCGCAY 1 cut(s) 240
FspBI CTAG 1 cut(s) 230
FspI TGCGCA 1 cut(s) 240
GlaI GCGC 2 cut(s) 184, 240
GluI GCNGC 4 cut(s) 86, 247, 398, 506
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 2 cut(s) 270, 610
HapII CCGG 5 cut(s) 25, 62, 77, 607, 651
HgaI GACGC 2 cut(s) 635, 644
HhaI GCGC 2 cut(s) 185, 241
Hin1II CATG 2 cut(s) 161, 674
Hin6I GCGC 2 cut(s) 183, 239
HinP1I GCGC 2 cut(s) 183, 239
HinfI GANTC 1 cut(s) 71
HpaII CCGG 5 cut(s) 25, 62, 77, 607, 651
HphI GGTGA 3 cut(s) 107, 362, 638
Hpy166II GTNNAC 1 cut(s) 490
Hpy188I TCNGA 2 cut(s) 529, 556
Hpy188III TCNNGA 2 cut(s) 25, 62
Hpy8I GTNNAC 1 cut(s) 490
HpyAV CCTTC 2 cut(s) 173, 341
HpyCH4III ACNGT 2 cut(s) 115, 487
HpyCH4V TGCA 5 cut(s) 227, 246, 283, 397, 432
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 463
HpyF3I CTNAG 3 cut(s) 501, 591, 597
Hsp92II CATG 2 cut(s) 161, 674
HspAI GCGC 2 cut(s) 183, 239
Kpn2I TCCGGA 2 cut(s) 24, 61
Kzo9I GATC 4 cut(s) 5, 21, 27, 58
LmnI GCTCC 2 cut(s) 162, 463
LpnPI CCDG 6 cut(s) 38, 75, 90, 269, 620, 664
Lsp1109I GCAGC 3 cut(s) 258, 409, 492
MaeI CTAG 1 cut(s) 230
MaeIII GTNAC 4 cut(s) 95, 335, 350, 632
MalI GATC 4 cut(s) 7, 23, 29, 60
MboI GATC 4 cut(s) 5, 21, 27, 58
MboII GAAGA 3 cut(s) 109, 165, 594
MhlI GDGCHC 2 cut(s) 167, 468
MlsI TGGCCA 1 cut(s) 270
MluCI AATT 4 cut(s) 278, 391, 452, 680
MluNI TGGCCA 1 cut(s) 270
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 1 cut(s) 130
MnlI CCTC 6 cut(s) 75, 510, 524, 549, 576, 617
Mox20I TGGCCA 1 cut(s) 270
MroI TCCGGA 2 cut(s) 24, 61
MroXI GAANNNNTTC 2 cut(s) 534, 561
MscI TGGCCA 1 cut(s) 270
MseI TTAA 2 cut(s) 288, 479
Msp20I TGGCCA 1 cut(s) 270
MspA1I CMGCKG 1 cut(s) 505
MspI CCGG 5 cut(s) 25, 62, 77, 607, 651
MspR9I CCNGG 3 cut(s) 78, 607, 651
MvnI CGCG 2 cut(s) 43, 69
MwoI GCNNNNNNNGC 2 cut(s) 82, 463
NciI CCSGG 3 cut(s) 78, 607, 651
NdeII GATC 4 cut(s) 5, 21, 27, 58
NlaIII CATG 2 cut(s) 161, 674
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 3 cut(s) 95, 350, 632
NsbI TGCGCA 1 cut(s) 240
NspI RCATGY 1 cut(s) 674
PdmI GAANNNNTTC 2 cut(s) 534, 561
PflFI GACNNNGTC 1 cut(s) 113
PfoI TCCNGGA 1 cut(s) 649
PkrI GCNGC 4 cut(s) 87, 248, 399, 507
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
Psp124BI GAGCTC 2 cut(s) 167, 468
PsyI GACNNNGTC 1 cut(s) 113
PvuII CAGCTG 1 cut(s) 505
SacI GAGCTC 2 cut(s) 167, 468
SaqAI TTAA 2 cut(s) 288, 479
SatI GCNGC 4 cut(s) 86, 247, 398, 506
Sau3AI GATC 4 cut(s) 5, 21, 27, 58
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 3 cut(s) 78, 607, 651
SduI GDGCHC 2 cut(s) 167, 468
SpeI ACTAGT 1 cut(s) 229
Sse9I AATT 4 cut(s) 278, 391, 452, 680
SsiI CCGC 2 cut(s) 41, 85
SspMI CTAG 1 cut(s) 230
SstI GAGCTC 2 cut(s) 167, 468
StyD4I CCNGG 3 cut(s) 76, 605, 649
TaaI ACNGT 2 cut(s) 115, 487
TaqI TCGA 3 cut(s) 57, 126, 313
TasI AATT 4 cut(s) 278, 391, 452, 680
TauI GCSGC 1 cut(s) 88
Tru1I TTAA 2 cut(s) 288, 479
Tru9I TTAA 2 cut(s) 288, 479
TseFI GTSAC 3 cut(s) 95, 350, 632
TseI GCWGC 3 cut(s) 246, 397, 505
Tsp45I GTSAC 3 cut(s) 95, 350, 632
TspDTI ATGAA 2 cut(s) 360, 675
Tth111I GACNNNGTC 1 cut(s) 113
XapI RAATTY 1 cut(s) 391
XceI RCATGY 1 cut(s) 674
XmnI GAANNNNTTC 2 cut(s) 534, 561
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.