Rroxscaffold_1G00035920

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
N/A
Physical Location & Seq
Reverse (-)
52376756 .. 52377127
372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00035920.1

Sequence Viewer

Length: 372 bp
ATGTCACCGCCGTGTACTCGGAAACGGGATTCGAAGCCTGATTTCCTGGCCACGGTGGACATGGATCCAAATTCTCCAACTTATTCCAAAGTTTTCCACACTGGCTTAGCTAACTTGGCAAAGCTGCCACAATATGGTCAATGGGTAGTCTCTCATGGCTTCCATGGTGATCCAAACCTCCTCAACTTCTACTCCTCCTCCTCCACCTTGAACCATCCAATAAAATATGATGTTTTTCTCAGTTTTAGAGGTGCGGACACCCGCACAAAGTTTACAGACCATTTATACAAGGAATTACATGTTCGGGAAGGCATCCCCACCTTTAGGGATTCGAAGAGCTCGAGAAGGAGAAGATCTTTCACAACTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

123

Amino Acids

14.14

Weight (kDa)

9.81

Isoelectric Point (pI)

48.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 75 - 114 4.4e-08 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 134
AciI CCGC 3 cut(s) 8, 254, 262
AclWI GGATC 3 cut(s) 59, 72, 164
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 1 cut(s) 70
AfaI GTAC 1 cut(s) 16
AfiI CCNNNNNNNGG 3 cut(s) 52, 134, 324
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 1 cut(s) 211
AjnI CCWGG 1 cut(s) 45
AleI CACNNNNGTG 1 cut(s) 10
AluBI AGCT 3 cut(s) 110, 124, 339
AluI AGCT 3 cut(s) 110, 124, 339
Alw21I GWGCWC 1 cut(s) 341
Alw26I GTCTC 1 cut(s) 154
AlwI GGATC 3 cut(s) 59, 72, 164
Ama87I CYCGRG 1 cut(s) 340
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 124
ApoI RAATTY 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 179
AsuII TTCGAA 2 cut(s) 32, 332
AvaI CYCGRG 1 cut(s) 340
BalI TGGCCA 1 cut(s) 50
BamHI GGATCC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 341
BbvI GCAGC 1 cut(s) 111
BccI CCATC 1 cut(s) 222
BciT130I CCWGG 1 cut(s) 47
BcoDI GTCTC 1 cut(s) 154
BglII AGATCT 1 cut(s) 353
BisI GCNGC 1 cut(s) 125
BlpI GCTNAGC 1 cut(s) 106
BlsI GCNGC 1 cut(s) 126
Bme1390I CCNGG 1 cut(s) 47
BmeT110I CYCGRG 1 cut(s) 340
BmiI GGNNCC 1 cut(s) 66
BmrFI CCNGG 1 cut(s) 47
BmsI GCATC 1 cut(s) 321
Bpu1102I GCTNAGC 1 cut(s) 106
Bpu14I TTCGAA 2 cut(s) 32, 332
BsaJI CCNNGG 2 cut(s) 51, 163
BsaXI ACNNNNNCTCC 4 cut(s) 176, 182, 206, 212
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 134, 324
Bse1I ACTGG 1 cut(s) 106
BseBI CCWGG 1 cut(s) 47
BseDI CCNNGG 2 cut(s) 51, 163
BseGI GGATG 2 cut(s) 214, 312
BseLI CCNNNNNNNGG 3 cut(s) 52, 134, 324
BseMII CTCAG 1 cut(s) 253
BseNI ACTGG 1 cut(s) 106
BseRI GAGGAG 4 cut(s) 170, 184, 187, 190
BseXI GCAGC 1 cut(s) 111
BshFI GGCC 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 341
BsiHKCI CYCGRG 1 cut(s) 340
BslI CCNNNNNNNGG 3 cut(s) 52, 134, 324
BsmAI GTCTC 1 cut(s) 154
BsnI GGCC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 340
Bsp119I TTCGAA 2 cut(s) 32, 332
Bsp1286I GDGCHC 1 cut(s) 341
Bsp143I GATC 3 cut(s) 64, 169, 353
Bsp1720I GCTNAGC 1 cut(s) 106
Bsp19I CCATGG 1 cut(s) 163
BspACI CCGC 3 cut(s) 8, 254, 262
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 252
BspLI GGNNCC 1 cut(s) 66
BspPI GGATC 3 cut(s) 59, 72, 164
BspQI GCTCTTC 1 cut(s) 329
BspT104I TTCGAA 2 cut(s) 32, 332
BsrI ACTGG 1 cut(s) 106
BssECI CCNNGG 2 cut(s) 51, 163
BssMI GATC 3 cut(s) 64, 169, 353
BssT1I CCWWGG 1 cut(s) 163
Bst2UI CCWGG 1 cut(s) 47
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 1 cut(s) 329
BstBI TTCGAA 2 cut(s) 32, 332
BstDEI CTNAG 2 cut(s) 106, 239
BstDSI CCRYGG 2 cut(s) 51, 163
BstF5I GGATG 2 cut(s) 214, 312
BstKTI GATC 3 cut(s) 67, 172, 356
BstMAI GTCTC 1 cut(s) 154
BstMBI GATC 3 cut(s) 64, 169, 353
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNI CCWGG 1 cut(s) 47
BstNSI RCATGY 1 cut(s) 302
BstSCI CCNGG 1 cut(s) 45
BstV1I GCAGC 1 cut(s) 111
BstX2I RGATCY 2 cut(s) 64, 353
BstYI RGATCY 2 cut(s) 64, 353
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 2 cut(s) 51, 163
BtsCI GGATG 2 cut(s) 214, 312
BtsIMutI CAGTG 1 cut(s) 99
Csp6I GTAC 1 cut(s) 15
CviAII CATG 4 cut(s) 61, 155, 164, 299
CviJI RGCY 7 cut(s) 37, 50, 105, 110, 124, 159, 339
CviKI_1 RGCY 7 cut(s) 37, 50, 105, 110, 124, 159, 339
CviQI GTAC 1 cut(s) 15
DdeI CTNAG 2 cut(s) 106, 239
DpnI GATC 3 cut(s) 66, 171, 355
DpnII GATC 3 cut(s) 64, 169, 353
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
Ecl136II GAGCTC 1 cut(s) 339
Eco130I CCWWGG 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 341
Eco53kI GAGCTC 1 cut(s) 339
Eco88I CYCGRG 1 cut(s) 340
EcoICRI GAGCTC 1 cut(s) 339
EcoRII CCWGG 1 cut(s) 45
EcoT14I CCWWGG 1 cut(s) 163
EcoT38I GRGCYC 1 cut(s) 341
ErhI CCWWGG 1 cut(s) 163
FaeI CATG 4 cut(s) 64, 158, 167, 302
FaiI YATR 7 cut(s) 62, 135, 156, 165, 228, 286, 300
FatI CATG 4 cut(s) 60, 154, 163, 298
FauI CCCGC 1 cut(s) 269
Fnu4HI GCNGC 1 cut(s) 125
FokI GGATG 2 cut(s) 201, 299
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 1 cut(s) 125
GluI GCNGC 1 cut(s) 125
HaeIII GGCC 1 cut(s) 50
Hin1II CATG 4 cut(s) 64, 158, 167, 302
HinfI GANTC 2 cut(s) 29, 329
HphI GGTGA 1 cut(s) 179
Hpy166II GTNNAC 3 cut(s) 15, 58, 273
Hpy188I TCNGA 1 cut(s) 21
Hpy188III TCNNGA 2 cut(s) 305, 342
Hpy8I GTNNAC 3 cut(s) 15, 58, 273
HpyAV CCTTC 2 cut(s) 302, 339
HpyCH4III ACNGT 1 cut(s) 55
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 2 cut(s) 106, 239
Hsp92II CATG 4 cut(s) 64, 158, 167, 302
Kzo9I GATC 3 cut(s) 64, 169, 353
LguI GCTCTTC 1 cut(s) 329
LpnPI CCDG 4 cut(s) 32, 51, 59, 87
Lsp1109I GCAGC 1 cut(s) 111
LweI GCATC 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 3
MalI GATC 3 cut(s) 66, 171, 355
MboI GATC 3 cut(s) 64, 169, 353
MboII GAAGA 2 cut(s) 346, 363
MflI RGATCY 2 cut(s) 64, 353
MhlI GDGCHC 1 cut(s) 341
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 2 cut(s) 70, 293
MluNI TGGCCA 1 cut(s) 50
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 6 cut(s) 188, 191, 205, 208, 211, 242
Mox20I TGGCCA 1 cut(s) 50
MscI TGGCCA 1 cut(s) 50
MslI CAYNNNNRTG 1 cut(s) 10
Msp20I TGGCCA 1 cut(s) 50
MspR9I CCNGG 1 cut(s) 47
MvaI CCWGG 1 cut(s) 47
MwoI GCNNNNNNNGC 1 cut(s) 116
NcoI CCATGG 1 cut(s) 163
NdeII GATC 3 cut(s) 64, 169, 353
NlaIII CATG 4 cut(s) 64, 158, 167, 302
NlaIV GGNNCC 1 cut(s) 66
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 302
NspV TTCGAA 2 cut(s) 32, 332
OliI CACNNNNGTG 1 cut(s) 10
PaeR7I CTCGAG 1 cut(s) 340
PciI ACATGT 1 cut(s) 298
PciSI GCTCTTC 1 cut(s) 329
PcsI WCGNNNNNNNCGW 1 cut(s) 338
PfeI GAWTC 2 cut(s) 29, 329
PflMI CCANNNNNTGG 1 cut(s) 134
PkrI GCNGC 1 cut(s) 126
PscI ACATGT 1 cut(s) 298
Psp124BI GAGCTC 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 45
PspGI CCWGG 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 66
PsuI RGATCY 2 cut(s) 64, 353
RsaI GTAC 1 cut(s) 16
RsaNI GTAC 1 cut(s) 15
RseI CAYNNNNRTG 1 cut(s) 10
SacI GAGCTC 1 cut(s) 341
SapI GCTCTTC 1 cut(s) 329
SatI GCNGC 1 cut(s) 125
Sau3AI GATC 3 cut(s) 64, 169, 353
ScrFI CCNGG 1 cut(s) 47
SduI GDGCHC 1 cut(s) 341
SetI ASST 7 cut(s) 112, 126, 180, 209, 253, 323, 341
SfaNI GCATC 1 cut(s) 321
Sfr274I CTCGAG 1 cut(s) 340
SfuI TTCGAA 2 cut(s) 32, 332
SlaI CTCGAG 1 cut(s) 340
SmiMI CAYNNNNRTG 1 cut(s) 10
SmlI CTYRAG 1 cut(s) 340
SmoI CTYRAG 1 cut(s) 340
Sse9I AATT 2 cut(s) 70, 293
SsiI CCGC 3 cut(s) 8, 254, 262
SstI GAGCTC 1 cut(s) 341
StyD4I CCNGG 1 cut(s) 45
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 1 cut(s) 55
TaqI TCGA 3 cut(s) 32, 332, 341
TasI AATT 2 cut(s) 70, 293
TatI WGTACW 1 cut(s) 14
TfiI GAWTC 2 cut(s) 29, 329
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 124
Tsp45I GTSAC 1 cut(s) 3
TspRI CASTG 1 cut(s) 106
Van91I CCANNNNNTGG 1 cut(s) 134
XapI RAATTY 1 cut(s) 70
XceI RCATGY 1 cut(s) 302
XcmI CCANNNNNNNNNTGG 1 cut(s) 58
XhoI CTCGAG 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.